{"title":"葡萄灰比诺病毒（GPGV）中国分离株的全基因组测序和感染性 cDNA 克隆构建","authors":"Qingyun Yuan, Ying Zhang, Fang Ren, Guojun Hu, Xudong Fan, Yafeng Dong","doi":"10.1007/s42161-024-01737-6","DOIUrl":null,"url":null,"abstract":"<p>The complete nucleotide sequence of a Chinese isolate C12B1 of grapevine Pinot gris virus (GPGV) from a ‘Beta’ grapevine was determined by small RNA sequencing. The size of the C12B1 genome was 7259 nucleotides (nt) without the poly (A) tail. Sequence identities of C12B1 with other GPGV isolates ranged from 79.13 to 97.52%. Phylogenetic analysis based on complete genome sequences of the global GPGV isolates indicated GPGV isolates are divided into three major groups and five subgroups within group 3, with the C12B1 isolate felled within subgroup 3a of group 3. Moreover, an infectious cDNA clone of the GPGV isolate C12B1 was generated using the seamless assembly approach. The infectious clone was then inoculated to <i>Nicotiana occidentalis</i> 37B and grapevine plants. Inoculated Nicotiana plants showed leaf chlorotic mottling symptoms and RT-PCR and transmission electron microscopy analysis confirmed successful infection. Additionally, the inoculated Beta grapevine plants also exhibited mild chlorotic mottling and ring spot symptoms. This study represents the first complete genome sequence of GPGV in China and the successful production of an infectious cDNA clone of a divergent GPGV isolate. These findings are a foundation for further research on the interaction between GPGV and its plant hosts.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"9 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Complete genome sequencing and infectious cDNA clone construction of a Chinese isolate of grapevine Pinot gris virus (GPGV)\",\"authors\":\"Qingyun Yuan, Ying Zhang, Fang Ren, Guojun Hu, Xudong Fan, Yafeng Dong\",\"doi\":\"10.1007/s42161-024-01737-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The complete nucleotide sequence of a Chinese isolate C12B1 of grapevine Pinot gris virus (GPGV) from a ‘Beta’ grapevine was determined by small RNA sequencing. The size of the C12B1 genome was 7259 nucleotides (nt) without the poly (A) tail. Sequence identities of C12B1 with other GPGV isolates ranged from 79.13 to 97.52%. Phylogenetic analysis based on complete genome sequences of the global GPGV isolates indicated GPGV isolates are divided into three major groups and five subgroups within group 3, with the C12B1 isolate felled within subgroup 3a of group 3. Moreover, an infectious cDNA clone of the GPGV isolate C12B1 was generated using the seamless assembly approach. The infectious clone was then inoculated to <i>Nicotiana occidentalis</i> 37B and grapevine plants. Inoculated Nicotiana plants showed leaf chlorotic mottling symptoms and RT-PCR and transmission electron microscopy analysis confirmed successful infection. Additionally, the inoculated Beta grapevine plants also exhibited mild chlorotic mottling and ring spot symptoms. This study represents the first complete genome sequence of GPGV in China and the successful production of an infectious cDNA clone of a divergent GPGV isolate. These findings are a foundation for further research on the interaction between GPGV and its plant hosts.</p>\",\"PeriodicalId\":16837,\"journal\":{\"name\":\"Journal of Plant Pathology\",\"volume\":\"9 1\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-08-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Plant Pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s42161-024-01737-6\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s42161-024-01737-6","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Complete genome sequencing and infectious cDNA clone construction of a Chinese isolate of grapevine Pinot gris virus (GPGV)
The complete nucleotide sequence of a Chinese isolate C12B1 of grapevine Pinot gris virus (GPGV) from a ‘Beta’ grapevine was determined by small RNA sequencing. The size of the C12B1 genome was 7259 nucleotides (nt) without the poly (A) tail. Sequence identities of C12B1 with other GPGV isolates ranged from 79.13 to 97.52%. Phylogenetic analysis based on complete genome sequences of the global GPGV isolates indicated GPGV isolates are divided into three major groups and five subgroups within group 3, with the C12B1 isolate felled within subgroup 3a of group 3. Moreover, an infectious cDNA clone of the GPGV isolate C12B1 was generated using the seamless assembly approach. The infectious clone was then inoculated to Nicotiana occidentalis 37B and grapevine plants. Inoculated Nicotiana plants showed leaf chlorotic mottling symptoms and RT-PCR and transmission electron microscopy analysis confirmed successful infection. Additionally, the inoculated Beta grapevine plants also exhibited mild chlorotic mottling and ring spot symptoms. This study represents the first complete genome sequence of GPGV in China and the successful production of an infectious cDNA clone of a divergent GPGV isolate. These findings are a foundation for further research on the interaction between GPGV and its plant hosts.
期刊介绍：
The Journal of Plant Pathology (JPP or JPPY) is the main publication of the Italian Society of Plant Pathology (SiPAV), and publishes original contributions in the form of full-length papers, short communications, disease notes, and review articles on mycology, bacteriology, virology, phytoplasmatology, physiological plant pathology, plant-pathogeninteractions, post-harvest diseases, non-infectious diseases, and plant protection. In vivo results are required for plant protection submissions. Varietal trials for disease resistance and gene mapping are not published in the journal unless such findings are already employed in the context of strategic approaches for disease management. However, studies identifying actual genes involved in virulence are pertinent to thescope of the Journal and may be submitted. The journal highlights particularly timely or novel contributions in its Editors’ choice section, to appear at the beginning of each volume. Surveys for diseases or pathogens should be submitted as "Short communications".
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