Journal of plant physiology最新文献

{"title":"Sucrose supplements modulate the Pseudomonas chlororaphis-Arabidopsis thaliana interaction via decreasing the production of phenazines and enhancing the root auxin response","authors":"Hugo Alejandro Tinoco-Tafolla ,&nbsp;José López-Hernández ,&nbsp;Randy Ortiz-Castro ,&nbsp;José López-Bucio ,&nbsp;Homero Reyes de la Cruz ,&nbsp;Jesús Campos-García ,&nbsp;Jesús Salvador López-Bucio","doi":"10.1016/j.jplph.2024.154259","DOIUrl":"https://doi.org/10.1016/j.jplph.2024.154259","url":null,"abstract":"<div><p>Management of the plant microbiome may help support food needs for the human population. Bacteria influence plants through enhancing nutrient uptake, metabolism, photosynthesis, biomass production and/or reinforcing immunity. However, information into how these microbes behave under different growth conditions is missing. In this work, we tested how carbon supplements modulate the interaction of <em>Pseudomonas chlororaphis</em> with <em>Arabidopsis thaliana. P. chlororaphis</em> streaks strongly repressed primary root growth, lateral root formation and ultimately, biomass production. Noteworthy, increasing sucrose availability into the media from 0 to 2.4% restored plant growth and promoted lateral root formation in bacterized seedlings. This effect could not be observed by supplementing sucrose to leaves only, indicating that the interaction was strongly modulated by bacterial access to sugar. Total phenazine content decreased in the bacteria grown in high (2.4%) sucrose medium, and conversely, the expression of <em>phzH</em> and <em>pslA</em> genes were diminished by sugar supply. Pyocyanin antagonized the promoting effects of sucrose in lateral root formation and biomass production in inoculated seedlings, indicating that this virulence factor accounts for growth repression during the plant-bacterial interaction. Defence reporter transgenes <em>PR-1::GUS</em> and <em>LOX2::GUS</em> were induced in leaves, while the expression of the auxin-inducible, synthetic reporter gene <em>DR5::GUS</em> was enhanced in the roots of bacterized seedlings at low and high sucrose treatments, which suggests that growth/defence trade-offs in plants are critically modulated by <em>P. chlororaphis</em>. Collectively, our data suggest that bacterial carbon nutrition controls the outcome of the relation with plants.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"297 ","pages":"Article 154259"},"PeriodicalIF":4.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140822009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide characterization, transcriptome profiling, and functional analysis of the ALMT gene family in Medicago for aluminum resistance","authors":"Dehui Jin,&nbsp;Jinlong Chen,&nbsp;Yumeng Kang,&nbsp;Fang Yang,&nbsp;Dongwen Yu,&nbsp;Xiaoqing Liu,&nbsp;Chengcheng Yan,&nbsp;Zhenfei Guo,&nbsp;Yang Zhang","doi":"10.1016/j.jplph.2024.154262","DOIUrl":"https://doi.org/10.1016/j.jplph.2024.154262","url":null,"abstract":"<div><p>Aluminum (Al) is the major limiting factor affecting plant productivity in acidic soils. Al³⁺ ions exhibit increased solubility at a pH below 5, leading to plant root tip toxicity. Alternatively, plants can perceive very low concentrations of Al³⁺, and Al triggers downstream signaling even at pH 5.7 without causing Al toxicity. The ALUMINUM-ACTIVATED-MALATE-TRANSPORTER (ALMT) family members act as anion channels, with some regulating the secretion of malate from root apices to chelate Al, which is a crucial mechanism for plant Al resistance. To date, the role of the ALMT gene family within the legume <em>Medicago</em> species has not been fully characterized. In this study, we investigated the ALMT gene family in <em>M</em>. <em>sativa</em> and <em>M</em>. <em>truncatula</em> and identified 68 <em>MsALMTs</em> and 18 <em>MtALMTs</em>, respectively. Phylogenetic analysis classified these genes into five clades, and synteny analysis uncovered genuine paralogs and orthologs. The real-time quantitative reverse transcription PCR (qRT-PCR) analysis revealed that <em>MtALMT8</em>, <em>MtALMT9</em>, and <em>MtALMT15</em> in clade 2-2b are expressed in both roots and root nodules, and <em>MtALMT8</em> and <em>MtALMT9</em> are significantly upregulated by Al in root tips. We also observed that <em>MtALMT8</em> and <em>MtALMT9</em> can partially restore the Al sensitivity of <em>Atalmt1</em> in <em>Arabidopsis</em>. Moreover, transcriptome analysis examined the expression patterns of these genes in <em>M</em>. <em>sativa</em> in response to Al at both pH 5.7 and pH 4.6, as well as to protons, and found that Al and protons can independently induce some Al-resistance genes. Overall, our findings indicate that <em>MtALMT8</em> and <em>MtALMT9</em> may play a role in Al resistance, and highlight the resemblance between the ALMT genes in <em>Medicago</em> species and those in <em>Arabidopsis</em>.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"297 ","pages":"Article 154262"},"PeriodicalIF":4.3,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140823038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dehydration-responsive protein PpFAS1.3 in moss Physcomitrium patens plays a regulatory role in lipid metabolism","authors":"Zhenyu Qi ,&nbsp;Chen Liu ,&nbsp;Ning Wang ,&nbsp;Jipeng Cui ,&nbsp;Jia Hu ,&nbsp;Ruoqing Gu ,&nbsp;Le Meng ,&nbsp;Pan Wang ,&nbsp;Jianan Zhai ,&nbsp;Guanghou Shui ,&nbsp;Suxia Cui","doi":"10.1016/j.jplph.2024.154253","DOIUrl":"https://doi.org/10.1016/j.jplph.2024.154253","url":null,"abstract":"<div><p>Moss plants appear in the early stages of land colonization and possess varying degrees of dehydration tolerance. In this study, a protein called PpFAS1.3 was identified, which contains a fasciclin 1-like domain and is essential for the moss <em>Physcomitrium patens</em>' response to short-term rapid dehydration. When the FAS1.3 protein was knocked out, leafyshoots showed a significant decrease in tolerance to rapid dehydration, resulting in accelerated water loss and increased membrane leakage. Phylogenetic analysis suggests that PpFAS1.3 and its homologous proteins may have originated from bacteria and are specifically found in non-vascular plants like mosses and liverworts. As a dehydration-related protein, FAS1.3 plays a significant role in regulating lipid metabolism, particularly in the synthesis of free fatty acids (FFA) and the metabolism of two phospholipids, PC and PA. This discovery highlights the close connection between PpFAS1.3 and lipid metabolism, providing new insights into the molecular mechanisms underlying plant adaptation to stresses.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"297 ","pages":"Article 154253"},"PeriodicalIF":4.3,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140822398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systemic long-distance sulfur transport and its role in symbiotic root nodule protein turnover","authors":"Alina Siegl ,&nbsp;Leila Afjehi-Sadat ,&nbsp;Stefanie Wienkoop","doi":"10.1016/j.jplph.2024.154260","DOIUrl":"https://doi.org/10.1016/j.jplph.2024.154260","url":null,"abstract":"<div><p>Sulfur is an essential nutrient for all plants, but also crucial for the nitrogen fixing symbiosis between legumes and rhizobia. Sulfur limitation can hamper nodule development and functioning. Until now, it remained unclear whether sulfate uptake into nodules is local or mainly systemic via the roots, and if long-distance transport from shoots to roots and into nodules occurs. Therefore, this work investigates the systemic regulation of sulfur transportation in the model legume <em>Lotus japonicus</em> by applying stable isotope labeling to a split-root system. Metabolite and protein extraction together with mass spectrometry analyses were conducted to determine the plants molecular phenotype and relative isotope protein abundances. Data show that treatments of varying sulfate concentrations including the absence of sulfate on one side of a nodulated root was not affecting nodule development as long as the other side of the root system was provided with sufficient sulfate. Concentrations of shoot metabolites did not indicate a significant stress response caused by a lack of sulfur. Further, we did not observe any quantitative changes in proteins involved in biological nitrogen fixation in response to the different sulfate treatments. Relative isotope abundance of ³⁴S confirmed a long-distance transport of sulfur from one side of the roots to the other side and into the nodules. Altogether, these results provide evidence for a systemic long-distance transport of sulfur via the upper part of the plant to the nodules suggesting a demand driven sulfur distribution for the maintenance of symbiotic N-fixation.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"297 ","pages":"Article 154260"},"PeriodicalIF":4.3,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0176161724000919/pdfft?md5=904df9849ad2d324eb8380b299e3dd56&pid=1-s2.0-S0176161724000919-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140822561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A method for separating tonoplast from wheat","authors":"Hui Zhou,&nbsp;Mancang Zhang,&nbsp;Ying Chang,&nbsp;Cuizhu Feng,&nbsp;Yu Long","doi":"10.1016/j.jplph.2024.154258","DOIUrl":"10.1016/j.jplph.2024.154258","url":null,"abstract":"<div><p>Vacuoles account for 90% of plant cell volume and play important roles in maintaining osmotic pressure, storing metabolites and lysosomes, compartmentalizing harmful ions, and storing and reusing minerals. These functions closely relay on the ion channels and transporters located on the tonoplast. The separation of intact vacuoles from plant cells is the key technology utilized in the study of tonoplast-located ion channels and transporters. However, the current vacuole separation methods are available for <em>Arabidopsis</em> and some other dicotyledons but are lacking for monocot crops. In this study, we established a new method for the vacuole separation from wheat mesophyll cells and investigated the transmembrane proton flux of tonoplasts with non-invasive micro-test technology (NMT). Moreover, our study provides a technology for the study of vacuole functions in monocot crops.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"299 ","pages":"Article 154258"},"PeriodicalIF":4.3,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140796918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AabHLH48, a novel basic helix-loop-helix transcription factor from Adonis amurensis, promotes early flowering in Arabidopsis by activating FRUITFULL expression","authors":"Shuang Feng ,&nbsp;Lulu Ren ,&nbsp;Shengyue Dai ,&nbsp;Haoyun Wang ,&nbsp;Fan Zhang ,&nbsp;Aimin Zhou ,&nbsp;Bin Zhou ,&nbsp;Jingang Wang","doi":"10.1016/j.jplph.2024.154256","DOIUrl":"https://doi.org/10.1016/j.jplph.2024.154256","url":null,"abstract":"<div><p>Basic helix–loop–helix (bHLH) transcription factors play various important roles in plant growth and development. In this study, a <em>AabHLH48</em> was identified in the floral organ of <em>Adonis amurensis</em>, a perennial herb that can naturally complete flowering at extreme low temperatures. <em>AabHLH48</em> was widely expressed in various tissues or organs of <em>A</em>. <em>amurensis</em> and was localized in the nucleus. Overexpression of <em>AabHLH48</em> promotes early flowering in Arabidopsis under both photoperiod (12 h light/12 h dark and 16 h light/8 h dark) and temperature (22 and 18 °C) conditions. Transcriptome sequencing combined with quantitative real-time PCR analysis showed that overexpression of <em>AabHLH48</em> caused a general upregulation of genes involved in floral development in Arabidopsis, especially for <em>AtAGAMOUS-LIKE 8</em>/<em>FRUITFULL</em> (<em>AtAGL8/FUL</em>). The yeast one-hybrid assay revealed that AabHLH48 has transcriptional activating activity and can directly bind to the promoter region of <em>AtAGL8/FUL</em>. These results suggest that the overexpression of <em>AabHLH48</em> promoting early flowering in Arabidopsis is associated with the upregulated expression of <em>AtAGL8/FUL</em> activated by AabHLH48. This indicates that <em>AabHLH48</em> can serve as an important genetic resource for improving flowering-time control in other ornamental plants or crops.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"297 ","pages":"Article 154256"},"PeriodicalIF":4.3,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140639201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and molecular mechanisms of plant-root responses to iron toxicity","authors":"Guangjie Li ,&nbsp;Jinlin Wu ,&nbsp;Herbert J. Kronzucker ,&nbsp;Baohai Li ,&nbsp;Weiming Shi","doi":"10.1016/j.jplph.2024.154257","DOIUrl":"10.1016/j.jplph.2024.154257","url":null,"abstract":"<div><p>The chemical form and physiological activity of iron (Fe) in soil are dependent on soil pH and redox potential (Eh), and Fe levels in soils are frequently elevated to the point of causing Fe toxicity in plants, with inhibition of normal physiological activities and of growth and development. In this review, we describe how iron toxicity triggers important physiological changes, including nitric-oxide (NO)-mediated potassium (K⁺) efflux at the tips of roots and accumulation of reactive oxygen species (ROS) and reactive nitrogen (RNS) in roots, resulting in physiological stress. We focus on the root system, as the first point of contact with Fe in soil, and describe the key processes engaged in Fe transport, distribution, binding, and other mechanisms that are drawn upon to defend against high-Fe stress. We describe the root-system regulation of key physiological processes and of morphological development through signaling substances such as ethylene, auxin, reactive oxygen species, and nitric oxide, and discuss gene-expression responses under high Fe. We especially focus on studies on the physiological and molecular mechanisms in rice and Arabidopsis under high Fe, hoping to provide a valuable theoretical basis for improving the ability of crop roots to adapt to soil Fe toxicity.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"297 ","pages":"Article 154257"},"PeriodicalIF":4.3,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0176161724000889/pdfft?md5=f27877fd12578f311b7942066d94c22f&pid=1-s2.0-S0176161724000889-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140783913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vesicle formation-related protein CaSec16 and its ankyrin protein partner CaANK2B jointly enhance salt tolerance in pepper","authors":"Bentao Yan,&nbsp;Linyang Zhang,&nbsp;Kexin Jiao,&nbsp;Zhenze Wang,&nbsp;Kang Yong,&nbsp;Minghui Lu","doi":"10.1016/j.jplph.2024.154240","DOIUrl":"https://doi.org/10.1016/j.jplph.2024.154240","url":null,"abstract":"<div><p>Vesicle transport plays important roles in plant tolerance against abiotic stresses. However, the contribution of a vesicle formation related protein CaSec16 (COPII coat assembly protein Sec16-like) in pepper tolerance to salt stress remains unclear. In this study, we report that the expression of <em>CaSec16</em> was upregulated by salt stress. Compared to the control, the salt tolerance of pepper with <em>CaSec16</em>-silenced was compromised, which was shown by the corresponding phenotypes and physiological indexes, such as the death of growing point, the aggravated leaf wilting, the higher increment of relative electric leakage (REL), the lower content of total chlorophyll, the higher accumulation of dead cells, H₂O₂, malonaldehyde (MDA), and proline (Pro), and the inhibited induction of marker genes for salt-tolerance and vesicle transport. In contrast, the salt tolerance of pepper was enhanced by the transient overexpression of <em>CaSec16</em>. In addition, heterogeneously induced CaSec16 protein did not enhance the salt tolerance of <em>Escherichia coli</em>, an organism lacking the vesicle transport system. By yeast two-hybrid method, an ankyrin protein, CaANK2B, was identified as the interacting protein of CaSec16. The expression of <em>CaANK2B</em> showed a downward trend during the process of salt stress. Compared with the control, pepper plants with transient-overexpression of <em>CaANK2B</em> displayed increased salt tolerance, whereas those with <em>CaANK2B</em>-silenced exhibited reduced salt tolerance. Taken together, both the vesicle formation related protein CaSec16 and its interaction partner CaANK2B can improve the pepper tolerance to salt stress.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"296 ","pages":"Article 154240"},"PeriodicalIF":4.3,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140543024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-hormonal analysis and aquaporins regulation reveal new insights on drought tolerance in grapevine","authors":"Riccardo Braidotti ,&nbsp;Rachele Falchi ,&nbsp;Alberto Calderan ,&nbsp;Alessandro Pichierri ,&nbsp;Radomira Vankova ,&nbsp;Petre I. Dobrev ,&nbsp;Michaela Griesser ,&nbsp;Paolo Sivilotti","doi":"10.1016/j.jplph.2024.154243","DOIUrl":"https://doi.org/10.1016/j.jplph.2024.154243","url":null,"abstract":"<div><p>Disentangling the factors that foster the tolerance to water stress in plants could provide great benefits to crop productions. In a two-year experiment, two new PIWI (fungus resistant) grapevine varieties, namely Merlot Kanthus and Sauvignon Kretos (<em>Vitis</em> hybrids), grown in the field, were subjected to two different water regimes: weekly irrigated (IR) or not irrigated (NIR) for two months during the summer. The two varieties exhibited large differences in terms of performance under water-limiting conditions. In particular, Merlot Kanthus strongly decreased stem water potential (Ψ_s) under water shortage and Sauvignon Kretos maintained higher Ψ_s values accompanied by generally high stomatal conductance and net carbon assimilation, regardless of the treatment. We hypothesized differences in the hormonal profile that mediate most of the plant responses to stresses or in the regulation of the aquaporins that control the water transport in the leaves. In general, substantial differences were found in the abundance of different hormonal classes, with Merlot Kanthus reporting higher concentrations of cytokinins while Sauvignon Kretos higher concentrations of auxins, jasmonate and salicylic acid. Interestingly, under water stress conditions ABA modulation appeared similar between the two cultivars, while other hormones were differently modulated between the two varieties. Regarding the expression of aquaporin encoding genes, Merlot Kanthus showed a significant downregulation of <em>VvPIP2;1</em> and <em>VvTIP2;1</em> in leaves exposed to water stress. Both genes have probably a role in influencing leaf conductance, and <em>VvTIP2;1</em> has been correlated with stomatal conductance values. This evidence suggests that the two PIWI varieties are characterized by different behaviour in response to drought. Furthermore, the findings of the study may be generalized, suggesting the involvement of a complex hormonal cross-talk and aquaporins in effectively influencing plant performance under water shortage.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"296 ","pages":"Article 154243"},"PeriodicalIF":4.3,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140535877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AtHD2D is involved in regulating lateral root development and participates in abiotic stress response in Arabidopsis","authors":"Yueyang Chu ,&nbsp;Ruochen Duan ,&nbsp;Haoran Song ,&nbsp;Wenshuo Zhang ,&nbsp;Yuxuan Zhou ,&nbsp;Yutong Ma ,&nbsp;Xiaotong Yin ,&nbsp;Lining Tian ,&nbsp;Israel Ausin ,&nbsp;Zhaofen Han","doi":"10.1016/j.jplph.2024.154242","DOIUrl":"https://doi.org/10.1016/j.jplph.2024.154242","url":null,"abstract":"<div><p>Roots are essential to terrestrial plants, as their growth and morphology are crucial for plant development. The growth of the roots is affected and regulated by several internal and external environmental signals and metabolic pathways. Among them, chromatin modification plays an important regulatory role. In this study, we explore the potential roles of the histone deacetylase AtHD2D in root development and lay the foundation for further research on the biological processes and molecular mechanisms of AtHD2D in the future. Our study indicates that AtHD2D affects the root tip microenvironment homeostasis by affecting the gene transcription levels required to maintain the root tip microenvironment. In addition, we confirmed that AtHD2D is involved in regulating <em>Arabidopsis</em> lateral root development and further explained the possible role of AtHD2D in auxin-mediated lateral root development. AtHD2D can effectively enhance the resistance of <em>Arabidopsis thaliana</em> to abiotic stress. We believe that AtHD2D is involved in coping with abiotic stress by promoting the development of lateral roots. Overexpression of AtHD2D promotes the accumulation of reactive oxygen species (ROS) in roots, indicating that AtHD2D is also involved in developing lateral roots mediated by ROS. Previous studies have shown that the overexpression of AtHD2D can effectively enhance the resistance of <em>Arabidopsis thaliana</em> to abiotic stress. Based on our data, we believe that AtHD2D participates in the response to abiotic stress by promoting the development of lateral roots. AtHD2D-mediated lateral root development provides new ideas for studying the mechanism of HDAC protein in regulating root development.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"297 ","pages":"Article 154242"},"PeriodicalIF":4.3,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140549356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}