Journal of Pharmacology & Pharmacotherapeutics最新文献

{"title":"Allelic Variants in the Warfarin-related Genes VKORC1 and CYP2C9 in a Western Saudi Population","authors":"A. Bagher, Wedyan S. Alharbi, Lamees S. Gadi, Lenah S. Binmahfouz, Rawan H Hareeri","doi":"10.1177/0976500x231189351","DOIUrl":"https://doi.org/10.1177/0976500x231189351","url":null,"abstract":"To investigate the allele and genotype frequencies of the warfarin-related genes VKORC1 (-1639G>A), CYP2C9*2, and CYP2C9*3 among healthy Saudis. This cross-sectional study involved 125 unrelated healthy Saudis ages 18–60 years visiting the King Abdulaziz University Hospital (KAUH) in Jeddah, Western Saudi Arabia. The Oragene™ DNA saliva collection kits were used to collect and extract DNA from saliva samples. A polymerase chain reaction-restriction fragment length polymorphism analysis was used to detect the mutant alleles. Over 51.4% of the Saudi participants carried one or more mutant alleles. The frequency of the VKORC1 (-1639G>A) allele in Saudi was relatively high at 54.8%. The frequencies of the CYP2C9 allele were 19.6% and 54% for the CYP2C9*2 and CYP2C9*3 alleles, respectively, which are substantially more abundant than in other populations. The observed high frequencies of VKORC1 (-1639G>A) and CYP2C9*2 and CYP2C9*3 polymorphisms suggest that genetic testing should be considered before initiating warfarin therapy to predict the optimal initial dose of warfarin and minimize warfarin-related side effects.","PeriodicalId":16761,"journal":{"name":"Journal of Pharmacology & Pharmacotherapeutics","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45070715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-Acetyl-l-Cysteine Ameliorations RenalFunction Early After Renal Ischemia and Reperfusion; it is not Protective over a LongTerm under a High-Sodium Diet in Rats","authors":"R. C. Pereira, C. Romão, Beatriz Santos Geoffroy Corrêa, W. Dominguez, L. Furukawa","doi":"10.1177/0976500x231189327","DOIUrl":"https://doi.org/10.1177/0976500x231189327","url":null,"abstract":"To evaluate the early and late effects of N-acetyl-l-cysteine (NAC) treatment on renal ischemia and reperfusion (I/R) insult in adult Wistar rats influenced by chronic high sodium (HS) intake. Adult male Wistar rats (8 weeks of age) received an HS (8.0% NaCl) or normal sodium (NS; 1.3% NaCl) diet and NAC (600 mg/L) in drinking water or normal water. At 11 weeks of age, the rats underwent a renal I/R procedure. They followed for 10 weeks after I/R, at the 1st, 2nd, 4th, and 10th weeks, in which tail blood pressure (tBP) and renal function were evaluated. And renal renin gene expression was evaluated in the 10th week after I/R. During the study, it was observed that the tBP remained consistently higher in the HS-I/R+water group compared to the NS-I/R+water group. However, in the early weeks following I/R (1st, 2nd, and 4th weeks), the tBP was lower in the HS-I/R+NAC group than in the HS-I/R+water group. In the 10th week after I/R, the serum creatinine levels were higher in both the HS-I/R+NAC and NS-I/R+NAC groups compared to the HS-I/R+water and NS-I/R+water groups. Conversely, the creatinine clearance was higher in the HS-I/R+NAC group than in the HS-I/R+ group in the 2nd week following I/R. Additionally, the urinary protein levels were higher in the HS-I/R+NAC group than in the NS-I/R+NAC group in the 10th week after I/R. It was also observed that NAC treatment resulted in increased renal renin gene expression in the 10th week following I/R. After renal I/R in animals given HS, NAC treatment was initially effective in lowering blood pressure or increasing creatinine clearance. However, these positive effects did not persist over the long term, resulting in decreased kidney function and increased blood pressure. Furthermore, the renin-angiotensin-aldosterone system was increased by HS intake, and the benefits of the NS diet were less effective than those of the HS diet. Thus, NAC provides temporary protection only in the early stages following an insult.","PeriodicalId":16761,"journal":{"name":"Journal of Pharmacology & Pharmacotherapeutics","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65432638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rationalization of Antibiotic Prescription: Modulation of the Gut Microbiome and Possibilities of Minimizing the Risks for the Development of Antibiotic Resistance—A Narrative Review","authors":"Ashok Kumar, Aman Shukla, P. Shinu, ,. B. Mathew, Shashank Kailkhura, Pranjal Pratap Singh, Anroop B Nair","doi":"10.1177/0976500x231189341","DOIUrl":"https://doi.org/10.1177/0976500x231189341","url":null,"abstract":"Antimicrobial resistance (AMR) is a serious global health issue, and it is greatly influenced by the gut flora. The rationalization of antimicrobial doses in clinical studies is crucial for preventing AMR. This review analyzes how rationalization tactics affect AMR and gut microbiota in clinical studies. Studies that provided data on the use of antibiotics, AMR, or gastrointestinal microbiota were taken into account for the current review. The AMR rate was found to be low when healthy gut flora was maintained using various antibiotic rationalization techniques, such as limited use of antibiotics or targeted treatments. However, the effectiveness of these strategies differed based on the particular intervention, the research population, and the length of the course of therapy. The rationalization of antibiotic prescriptions in clinical research is one potential method for reducing the prevalence of AMR by maintaining the gut flora. Rationalization techniques may help lower AMR rates and foster the development of good intestinal flora. This review describes various antibiotic rationalization techniques and the importance of maintaining healthy microbial flora to minimize AMR-associated health issues.","PeriodicalId":16761,"journal":{"name":"Journal of Pharmacology & Pharmacotherapeutics","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47226366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Science of Antioxidants: Balancing thePros and Cons for Our Health","authors":"Mueen Ahmed K. K.","doi":"10.1177/0976500x231182792","DOIUrl":"https://doi.org/10.1177/0976500x231182792","url":null,"abstract":"","PeriodicalId":16761,"journal":{"name":"Journal of Pharmacology & Pharmacotherapeutics","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47246585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Bezafibrate, a PPARα Activator, on Acute and Subacute Inflammation in Male Wistar Rats","authors":"Serah Johny, S. Torgal","doi":"10.1177/0976500X231175218","DOIUrl":"https://doi.org/10.1177/0976500X231175218","url":null,"abstract":"Aim To investigate the anti-inflammatory effect of bezafibrate on acute and subacute inflammation in adult male Wistar rats. Methods The study was carried out in adult male Wistar rats and they were allocated into three groups, that is, control, aspirin and bezafibrate, after obtaining clearance from Institutional Animal Ethics Committee. Acute inflammation was studied using carrageenan-induced rat paw oedema, and the volume displacement due to paw oedema using the plethysmograph. Subacute inflammation was studied using foreign body induced granuloma models. Analysis was performed using one-way ANOVA followed by post hoc tests of Dunnett’s. The value p < 0.05 was considered statistically significant. Results Bezafibrate showed a significant anti-inflammatory effect in acute as well as subacute models of inflammation when compared to control in the present study. Conclusions In patients receiving bezafibrate for hyperlipidemia, its anti-inflammatory potential may have an additional benefit in preventing complication of atherosclerosis.","PeriodicalId":16761,"journal":{"name":"Journal of Pharmacology & Pharmacotherapeutics","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43041448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective Partial Agonism of Vasopressin 1a Receptors In Vitro by OCE-205","authors":"G. Croston, E. Cable, Jeannine Toy, Hiroe Tariga, R. Laporte, Geoffrey Harris, S. Bukofzer","doi":"10.1177/0976500X231175220","DOIUrl":"https://doi.org/10.1177/0976500X231175220","url":null,"abstract":"Objective To test the selectivity and degree of functional agonism of Ocelot Bio’s dual agonist/antagonist molecule, OCE-205, at the vasopressin 1a receptor (V1aR). Methods Cells expressing human (h) or rat V1a, V1b, V2, or oxytocin receptors (OTR) were incubated with varying concentrations of OCE-205 or with arginine vasopressin (AVP), and responses were measured with fluorescence or reporter gene assays. In addition, human resistance arteries were exposed to increasing concentrations of OCE-205, and the resulting contractility was measured. Results The mean efficacy of OCE-205 at hV1aR was 39% of the maximal possible effect (MPE), with a mean EC50 of 0.71 nM. Above 1 nM OCE-205, the percent maximal possible effect (%MPE) plateaued. The EC50 was much higher at hV1bR (134 nM), hV2R (420 nM), and OTR (6.9 nM), indicating selectivity for hV1aR. Results at rat receptors were similar. OCE-205 produced 40.0% of maximal depolarization-induced contraction, demonstrating functional partial agonism. Conclusion The dual agonist/antagonist structure of OCE-205 thus allows it to act as a highly selective partial agonist at vasopressin V1aR at therapeutically relevant concentrations.","PeriodicalId":16761,"journal":{"name":"Journal of Pharmacology & Pharmacotherapeutics","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42769858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence, Patterns, and Severity of Potential Drug Interactions Among Cancer Patients on Chemotherapy in a Tertiary Care Hospital","authors":"Anuradha H.V., V. Maka, Prakash Patil, A. C.","doi":"10.1177/0976500X231162712","DOIUrl":"https://doi.org/10.1177/0976500X231162712","url":null,"abstract":"Objective To study the incidence of potential drug−drug interactions (DDIs) and evaluate their pattern and severity in cancer inpatients. Materials and Methods A detailed clinical data and prescriptions of 150 inpatients with different malignancies were subjected to DDI screening using Micromedex software. The frequency of potential DDIs and their types, patterns, and severity were investigated. Results A total of 360 potential DDIs were present in 111 (74%) of 150 inpatients, dominated by female (67.33%) and breast cancer (30%) patients. The incidence of severe interactions was 63.88%, moderate interactions 35.83%, and mild interactions 0.27%. The potential mechanisms of DDIs were 38.33% pharmacodynamic, 48.33% pharmacokinetic, and 13.33% unspecified. The drug interactions were found to be positively correlated (p < 0.01) with the 6–10 number of prescribed medicines. Conclusion According to this study, the number of medicines prescribed to cancer inpatients increased the chance of DDIs. As a result, the drug surveillance program could save a sizable number of patients from the potentially hazardous clinical effects of DDIs.","PeriodicalId":16761,"journal":{"name":"Journal of Pharmacology & Pharmacotherapeutics","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49166303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Observational Study on the Intraoperative Awareness Following General Anesthetic Drugs","authors":"S. Priyadharsini K., Nikita Haldar, K. Prasad T, Mali Kalpana Ramanna, Maduram Annamalai","doi":"10.1177/0976500X231172579","DOIUrl":"https://doi.org/10.1177/0976500X231172579","url":null,"abstract":"Objective To determine intraoperative awareness and estimate the factors associated with intraoperative awareness. Methods An observational cross-sectional study was carried out after approval from the institutional ethical committee. The duration of the study is 2 months. After completion of the surgical procedure, anesthesia was reversed, and there was an adequate return of consciousness. The patients were interviewed about their surgery using the modified form of the Brice questionnaire. After the questionnaire was completed, it was analyzed, and patients were categorized into either having definite awareness, possible awareness, or no awareness. Results It was observed that out of 90 patients operated on under general anesthesia, eight reported having remembered something under general anesthesia. Out of eight patients, two were found to have definite awareness, and the occurrence of definite awareness was calculated to be 2.22%. Six patients were categorized under possible awareness, and the occurrence of possible awareness was estimated to be 6.6% in our study. Conclusion The occurrence of intraoperative awareness was estimated to be 8.8%, including definite and possible awareness under general anesthesia. Finally, we conclude that intraoperative awareness might be due to the inadequate depth of general anesthesia given to the patients. The dose of general anesthetic drugs was not maintained based on its minimum alveolar concentration (MAC).","PeriodicalId":16761,"journal":{"name":"Journal of Pharmacology & Pharmacotherapeutics","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49184796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotics: A Review on Microbiome That Helps for Better Health – A Dermatologist’s Perspective","authors":"S. Renuka, N. A. Kumar, D. Manoharan, Dama Kondaiah Naidu","doi":"10.1177/0976500X231175225","DOIUrl":"https://doi.org/10.1177/0976500X231175225","url":null,"abstract":"Commensal microorganisms heavily influence human health and disease pathogenesis, and the impact of the skin microbiome on numerous skin illnesses has recently piqued researchers’ interest, in addition to the gut microbiome. Probiotics are live microbial organisms that are good for the host’s health when given in sufficient proportions. The concept of probiotics has sparked much curiosity and scientific investigation since its inception. Probiotics alter the intestinal microbiota and are employed as a treatment technique for a variety of diseases. Despite several studies, the significance of probiotics in dermatological illnesses such as acne, vaginal infections, and atopic dermatitis has yet to be demonstrated. Evidence pointing to the “gut-skin axis” link has increased, and intestinal microbiota regulation may play a role in dermatological diseases. There is, however, no consensus on the species or the dosage to be utilized for such therapies. This article makes an effort to review recent evidence from the literature.","PeriodicalId":16761,"journal":{"name":"Journal of Pharmacology & Pharmacotherapeutics","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45959822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Virtual Reality High-Fidelity Adult Mannequin-based Simulation of Real-Life Clinical Scenarios in Teaching Clinical Pharmacology to Medical Students","authors":"Anupama M. Gudadappanavar, P. Hombal, Jyoti M. Benni, Sachin Patel, B. Tubaki","doi":"10.1177/0976500X231159456","DOIUrl":"https://doi.org/10.1177/0976500X231159456","url":null,"abstract":"Background Presently, competency-based medical (CBME) curriculum challenges the medical educators to provide continuing education with new approaches to make learning more stimulating, motivating, and entertaining, fostering excellence in clinical practice. To address these issues, educators have advocated the use of virtual reality and mannequins to teach clinical pharmacology. Objectives To study the effect, perception, and feedback of virtual reality high-fidelity adult mannequin-based (VHFM) simulation of real-life clinical scenarios over conventional tutorials in teaching clinical pharmacology to medical students. Material and Methods An interventional study was designed for 2nd year MBBS students for a period of 6 months. The enrolled students were randomly assigned to the test group (VHFM) or control group (tutorials). The CAE Healthcare Ltd. maestro, high-fidelity prehospital mannequin (APOLLO) was used in the test group. Three sessions consisting of six different cases were discussed, and multiple-choice questions (MCQs)-based pre-test, post–test, and retest after a month were conducted in both groups and compared. The perception and feedback of faculty and students were obtained by using a modified and revalidated questionnaire. Results The test group outperformed the control group (p <.001) in all sessions based on pre-test, post-test, and retest scores, and within-group comparisons revealed significant improvements in both groups. Students’ perceptions and feedback regarding VHFM were more enthusiastic and promising than in the tutorial group. Conclusions VHFM is student-centered, provides an active learning environment, and aids in skill development. We strongly suggest VHFM-based learning as a complement to traditional teaching strategies in pharmacology, especially for teaching clinical reasoning to medical students.","PeriodicalId":16761,"journal":{"name":"Journal of Pharmacology & Pharmacotherapeutics","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42213238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}