Facta Universitatis, Series: Medicine and Biology最新文献

FERMENTATION ENHANCES THE ANTIPROLIFERATIVE ACTIVITIES OF ANNONA SQUAMOSA SEED POLYSACCHARIDES ON HUMAN MCF-7 AND RHABDOMYOSARCOMA 发酵增强胭脂虫种子多糖对人类 mcf-7 和横纹肌肉瘤的抗增殖活性

Facta Universitatis, Series: Medicine and Biology Pub Date : 2024-07-27 DOI: 10.22190/fumb240126002d

C. Dare, B. Oluremi, T. E. Akinleye, J. Adeniji, M. Adewumi, O. Oyedapo

{"title":"FERMENTATION ENHANCES THE ANTIPROLIFERATIVE ACTIVITIES OF ANNONA SQUAMOSA SEED POLYSACCHARIDES ON HUMAN MCF-7 AND RHABDOMYOSARCOMA","authors":"C. Dare, B. Oluremi, T. E. Akinleye, J. Adeniji, M. Adewumi, O. Oyedapo","doi":"10.22190/fumb240126002d","DOIUrl":"https://doi.org/10.22190/fumb240126002d","url":null,"abstract":"Cancer is a primary cause of death globally and a significant public health concern. Polysaccharides are complex carbohydrates found abundantly in natural sources, including plants, and have attracted interest due to their possible health advantages, including anticancer activities. Annona squamosa, also known as the sugar apple, has long been prized for its healing properties, thus an intriguing candidate for investigating its potential effects on cancer cells. Through boosting bioavailability, producing active metabolites, enriching the nutrient profile, altering the composition of bioactive substances, and influencing the gut microbiota, fermentation plays a crucial role in promoting the antiproliferative effects of food. Polysaccharide was extracted from the fermented and unfermented cotyledon and coat of A. squamosa seed, characterised by HPLC, and antiproliferative activity was investigated using the MTT assay on human breast adenocarcinoma (MCF-7) and rhabdomyosarcoma (RD) cell lines. The Vero cell line obtained from the kidney of a green monkey of African descent was used for selectivity. The polysaccharides displayed antiproliferative activity against the cancerous cell lines MCF-7 (breast cancer) and RD (rhabdomyosarcoma-soft tissue sarcoma), with IC50 values ranging from 27.10 ± 0.61 to 57.01 ± 0.06 µg/ml and a good selectivity for the cancer cells over normal body cells (Vero), with fermented better than unfermented. In conclusion, A. squamosa seed polysaccharides exhibited antiproliferative properties on MCF-7 and RD, which could be explored in developing a novel drug in cancer prevention and treatment.","PeriodicalId":167216,"journal":{"name":"Facta Universitatis, Series: Medicine and Biology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141798069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

POPULATION PHARMACOKINETICS OF PANTOPRAZOLE IN PATIENTS ON DUAL ANTIPLATELET THERAPY FOLLOWING ACUTE CORONARY SYNDROME 急性冠状动脉综合征后接受双重抗血小板疗法的患者体内泮托拉唑的群体药代动力学

Facta Universitatis, Series: Medicine and Biology Pub Date : 2024-04-13 DOI: 10.22190/fumb240216001n

V. Nikolić, D. Stokanović, Slobodan M Janković, Sandra S. Konstatinović, J. Zvezdanović, Jelena Lilić, Nikola Stefanović, S. Apostolovic, J. Milovanović

{"title":"POPULATION PHARMACOKINETICS OF PANTOPRAZOLE IN PATIENTS ON DUAL ANTIPLATELET THERAPY FOLLOWING ACUTE CORONARY SYNDROME","authors":"V. Nikolić, D. Stokanović, Slobodan M Janković, Sandra S. Konstatinović, J. Zvezdanović, Jelena Lilić, Nikola Stefanović, S. Apostolovic, J. Milovanović","doi":"10.22190/fumb240216001n","DOIUrl":"https://doi.org/10.22190/fumb240216001n","url":null,"abstract":"This study investigates factors influencing pantoprazole pharmacokinetics (PK) in patients with acute coronary syndrome (ACS) undergoing dual antiplatelet therapy (DAPT) comprising acetylsalicylic acid and clopidogrel, alongside concomitant pantoprazole to mitigate gastrointestinal risks. We conducted a prospective analysis on 93 ACS patients, assessing pantoprazole PK parameters and their correlation with C-reactive protein (CRP) levels, indicative of inflammation. Blood samples for pantoprazole concentration and CRP levels were collected according to a predefined schedule, post-oral pantoprazole administration at steady state. The study highlights a notable influence of CRP levels on pantoprazole clearance, underscoring inflammation’s impact on drug metabolism. Elevated CRP was associated with altered pantoprazole pharmacokinetics, suggesting that inflammatory status significantly contributes to metabolic variability in this patient population. Our findings suggest the need for personalized pantoprazole dosing in ACS patients on DAPT, considering the inflammatory status as reflected by CRP levels. This approach could optimize therapeutic efficacy and minimize adverse effects, advancing personalized treatment strategies in the management of ACS.","PeriodicalId":167216,"journal":{"name":"Facta Universitatis, Series: Medicine and Biology","volume":"80 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140707863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE INFLUENCE OF THE APOEε4 ALLELE ON PRO-INFLAMMATORY CYTOKINE LEVELS IN THE CEREBROSPINAL FLUID OF PATIENTS WITH ALZHEIMER’S DISEASE APOEε4 基因对阿尔茨海默病患者髓鞘液中促炎症细胞激素水平的影响

Facta Universitatis, Series: Medicine and Biology Pub Date : 2024-01-17 DOI: 10.22190/fumb231012006b

Jelena Bašić, V. Milošević, B. Đorđević, Nikola Stefanović, Vladana Stojiljković, T. Jevtović Stoimenov, Ivana Stojanović

{"title":"THE INFLUENCE OF THE APOEε4 ALLELE ON PRO-INFLAMMATORY CYTOKINE LEVELS IN THE CEREBROSPINAL FLUID OF PATIENTS WITH ALZHEIMER’S DISEASE","authors":"Jelena Bašić, V. Milošević, B. Đorđević, Nikola Stefanović, Vladana Stojiljković, T. Jevtović Stoimenov, Ivana Stojanović","doi":"10.22190/fumb231012006b","DOIUrl":"https://doi.org/10.22190/fumb231012006b","url":null,"abstract":"Single nucleotide polymorphisms (SNPs) rs429358 and rs7412, the most commonly investigated variants in the apolipoprotein E gene (APOE) are crucial for APOEε4 carrier status determination. However, their association with inflammatory cytokine levels in patients with dementia due to Alzheimer’s disease (AD) remains unclear. This study aimed to investigate the influence of the APOEε4 allele on pro-inflammatory cytokine levels in the cerebrospinal fluid of patients with AD dementia. The research was conducted on 36 patients with probable dementia due to AD. APOE rs429358 and rs7412 were analyzed using the Real-Time PCR method with allele-specific TaqMan assays, followed by the analysis of APOEε4 allele carrier status. Patients carrying at least one APOEε4 allele were assigned as APOEε4+. Core biomarkers (Aβ42/40 ratio, t-Tau, p-Tau levels), as well as pro-inflammatory cytokine (Tumor necrosis factor-a (TNF-a) and interleukin-1b (IL-1b)) levels, were determined in the patient’s cerebrospinal fluid (CSF) using Enzyme-linked Immunosorbent Assay (ELISA). Seventeen patients (47.22%) were assigned as APOEε4+. CSF TNF-α levels were significantly higher in APOEε4+ AD dementia patients in comparison to APOEε4- patients (p<0.001), while no significant differences in IL-1b levels between these two groups were obtained. Correlation analysis showed that TNF-α negatively correlated with the Aβ42/40 ratio (p=0.033), while positive correlation with t-Tau and p-Tau was observed (p=0.001, p=0.015, respectively). These findings highlight the potential significance of TNF-α in the context of APOEε4 positivity and its implications in AD pathology.","PeriodicalId":167216,"journal":{"name":"Facta Universitatis, Series: Medicine and Biology","volume":"55 28","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139527827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ANTIOXIDANTS SUPPLEMENTATION BASED ON NATURAL PRODUCTS IN THE TREATMENT OF THE THERMAL SKIN INJURY 在治疗皮肤热损伤时补充基于天然产品的抗氧化剂

Facta Universitatis, Series: Medicine and Biology Pub Date : 2023-12-29 DOI: 10.22190/fumb230919007k

Miloš Krstić, Bojana Krstić

{"title":"ANTIOXIDANTS SUPPLEMENTATION BASED ON NATURAL PRODUCTS IN THE TREATMENT OF THE THERMAL SKIN INJURY","authors":"Miloš Krstić, Bojana Krstić","doi":"10.22190/fumb230919007k","DOIUrl":"https://doi.org/10.22190/fumb230919007k","url":null,"abstract":"Thermal injuries are a major medical, economic and social problem, whose severity, morbidity, mortality, prognosis and treatment outcome directly depend on the surface and depth of the burn, but also on the patient's age, health status and accompanying complications. The treatment of thermal injury is long and complex, and the outcome is often uncertain with a very complex rehabilitation process, because the injury damages both physical and psychological health. Constant innovations in the protocol of medical treatment and the search for new mechanisms involved in the pathogenesis of burns can be helpful in successful treatment. Therefore, there is a constant search for adjuvant therapies that will improve results like HBO, AO, platelet- rich plasma, negative pressure wound therapy and others. In recent years, the application of antioxidants as adjuvant therapy for the care and treatment of wounds has been the subject of various researche. Antioxidants are biologically active molecules that prevent the creation of free radicals in the body and at the same time neutralize the existing ones, this way participating in maintaining the oxidative status at the desired level. Antioxidant -rich natural products may provide a solution to improve wound healing. The application of antioxidant therapy has shown numerous benefits in improving the outcome of burns. However, more information is needed from the clinical trials on the use of natural products, especially in combination with other therapies. Keywords: thermal skin injury, hyperbaric oxygen therapy, antioxidant rich natural products","PeriodicalId":167216,"journal":{"name":"Facta Universitatis, Series: Medicine and Biology","volume":" 37","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139144884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CYTOKINE PROFILE IN RENAL TRANSPLANT PATIENTS IN A LONG-TERM PERIOD AFTER SURGERY 肾移植术后长期内细胞因子的变化

Facta Universitatis, Series: Medicine and Biology Pub Date : 2023-06-21 DOI: 10.22190/fumb230406003s

Vladana Stojiljkovic, N. Stefanović, Katarina Danković, B. Đorđević, M. Cvetković, N. Stevic, Stevan Vujic, B. Mitić, T. Cvetković

{"title":"CYTOKINE PROFILE IN RENAL TRANSPLANT PATIENTS IN A LONG-TERM PERIOD AFTER SURGERY","authors":"Vladana Stojiljkovic, N. Stefanović, Katarina Danković, B. Đorđević, M. Cvetković, N. Stevic, Stevan Vujic, B. Mitić, T. Cvetković","doi":"10.22190/fumb230406003s","DOIUrl":"https://doi.org/10.22190/fumb230406003s","url":null,"abstract":"In the long-term period after kidney transplantation, a certain level of tissue inflammation and therefore the production of proinflammatory cytokines, including TNF-α, IL-1β, IL-18 and IL-2 can be found. The aim of our study was to determine the concentrations of TNF-α, IL-1β, IL-18, IL-2 and its soluble receptor (IL-2R) in renal transplant patients, regarding the length of the postoperative period. The study involved 65 patients, transplanted at least 12 months prior to our investigation, divided into three groups, regarding the time passed since the transplantation (12-24, 24-48, and >48 months consecutively). Concentrations of the cytokines in the plasma of the subjects were measured using ELISA method. Group I showed significantly higher concentrations of IL-1b compared to the III (p<0.05), IL-18 compared to the II and III (p<0.05) and TNF-a compared to the II (p<0.05). Cytokine concentrations correlated with the time passed since the transplantation (p<0.05), except for TNF-a. Interleukin-2 correlated negatively with IL-18 and immunosuppressant dosage (p<0.05). Interleukin-1b, IL-18 and TNF-α measurements should be considered for monitoring and detecting potentially subclinical allograft damage in the second year after surgery. However, the dynamics of the change of cytokine concentration may also have been altered by the components of the immunosuppressive protocols used, such as tacrolimus, which is a link that is yet to be examined.","PeriodicalId":167216,"journal":{"name":"Facta Universitatis, Series: Medicine and Biology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125229128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COULD IT BE BEHÇET'S DISEASE? PHYSICIANS IN PRIMARY HEALTHCARE ARE THE FIRST TO RESPOND 会不会是behÇet的病?初级卫生保健的医生是第一个做出反应的人

Facta Universitatis, Series: Medicine and Biology Pub Date : 2023-06-21 DOI: 10.22190/fumb221205001k

S. Knežević, Jelena Vulović, Marijana Jandrić-Kočić

{"title":"COULD IT BE BEHÇET'S DISEASE? PHYSICIANS IN PRIMARY HEALTHCARE ARE THE FIRST TO RESPOND","authors":"S. Knežević, Jelena Vulović, Marijana Jandrić-Kočić","doi":"10.22190/fumb221205001k","DOIUrl":"https://doi.org/10.22190/fumb221205001k","url":null,"abstract":"Behçet's disease is a chronic relapsing vasculitis with complex immunopathogenesis that can affect multiple organs, although mucocutaneous lesions are predominantly observed. Diagnosis of Behçet's disease is challenging and relies on clinical manifestations as no specific laboratory test is available. A positive Pathergy test should raise suspicion of Behçet's disease. The authors present a clinical case of a 22-year-old woman who presented with intermittent fever, weight loss, odynophagia, dysphagia, left knee arthritis, and multiple painful oral-genital aphthosis. The patient received treatment with benzydamine hydrochloride mouthwash, lidocaine on ulcers, antibiotics, and non-steroidal anti-inflammatory drugs, but the healing process was slow, and a new disease attack occurred two months later. The Pathergy test was performed and found to be positive, prompting consultation with an immunologist and gynecologist. The patient was administered Colchicine (1.5 mg/day), which resulted in decreased symptoms, albeit with a slow healing process. During the 6-month follow-up period, the disease attack was followed by a period of remission. The evidence base for the treatment of Behçet's disease is limited, and current options include symptomatic, non-biological, and biological immunosuppressive drugs to reduce clinical manifestations. A thorough evaluation and education in primary healthcare settings are crucial to facilitate timely multidisciplinary referrals for effective management of Behçet's disease.","PeriodicalId":167216,"journal":{"name":"Facta Universitatis, Series: Medicine and Biology","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130083989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SECONDARY HOST PLANTS OF WATER LILY APHID, RHOPALOSIPHUM NYMPHAEAE (HEMIPTERA: APHIDIDAE) IN SERBIA 塞尔维亚睡莲蚜(半翅目:蚜科)的次生寄主植物

Facta Universitatis, Series: Medicine and Biology Pub Date : 2023-02-21 DOI: 10.22190/fumb221117010m

Darija Milenković, V. Žikić, Saša S Stanković, Maja Lazarević, O. Petrović-Obradović, Marijana Ilić Milošević

引用次数: 0

ASSESSMENT OF THE INSTRUMENTAL ACTIVITIES OF DAILY LIVING IN MILD COGNITIVE IMPAIRMENT AND DEMENTIA DUE TO ALZHEIMER'S DISEASE: DIAGNOSTIC ACCURACY OF THE SERBIAN VERSION OF THE AMSTERDAM IADL QUESTIONNAIRE 对阿尔茨海默病引起的轻度认知障碍和痴呆患者日常生活工具性活动的评估:塞尔维亚版阿姆斯特丹iadl问卷的诊断准确性

Facta Universitatis, Series: Medicine and Biology Pub Date : 2023-01-06 DOI: 10.22190/fumb221215009m

V. Milošević, M. Malobabic, Eva Antić, Aleksandra Aracki Trenkić, D. Stojanov, J. Bašić

{"title":"ASSESSMENT OF THE INSTRUMENTAL ACTIVITIES OF DAILY LIVING IN MILD COGNITIVE IMPAIRMENT AND DEMENTIA DUE TO ALZHEIMER'S DISEASE: DIAGNOSTIC ACCURACY OF THE SERBIAN VERSION OF THE AMSTERDAM IADL QUESTIONNAIRE","authors":"V. Milošević, M. Malobabic, Eva Antić, Aleksandra Aracki Trenkić, D. Stojanov, J. Bašić","doi":"10.22190/fumb221215009m","DOIUrl":"https://doi.org/10.22190/fumb221215009m","url":null,"abstract":"To establish a diagnosis of dementia, it is necessary, in addition to cognitive impairment, to prove the existence of a disorder of instrumental activities of daily living (IADL). The Amsterdam IADL Questionnaire is a reliable instrument translated into different languages. This study aims to assess the diagnostic accuracy of the Serbian version of the Amsterdam IADL Questionnaire. The study included 75 patients with mild cognitive impairment (MCI) and dementia due to Alzheimer's disease. The questionnaire was scored using the weighted average (WA) and item response theory (IRT) scoring method. Diagnostic accuracy was examined using receiver–operating characteristic (ROC) curves. The area under the curves (AUC) was calculated with 95% confidence intervals (CI). The correlation between IRT and WA scores was strong and significant (r=-0.980, p<0.001). The AUC for the IRT scores of A-IADL-Q was 0.832 (95% CI: 0.729 to 0.909), while the AUC for the WA scores of A-IADL-Q was 0.848 (95% CI: 0.746 to 0.920). Both were significantly different from the AUC of 0.5 (p<0.001). There was no significant difference between the AUCs of IRT and WA scoring (z=1.157; p=0.247). Cutoffs and the highest combination of sensitivity and specificity for the IRT (sensitivity 0.767; specificity 0. 844) and WA (sensitivity 0.744; specificity 0. 844) scores of A-IADL-Q were calculated. We have shown that A-IADL-Q has moderate diagnostic accuracy in differentiating dementia and MCI. This instrument can be used in combination with cognitive measures to diagnose dementia in its early stages.","PeriodicalId":167216,"journal":{"name":"Facta Universitatis, Series: Medicine and Biology","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131647885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EWING SARCOMA FAMILY OF TUMORS OF SCAPULA IN CHILDREN 儿童肩胛骨肿瘤尤因肉瘤家族

Facta Universitatis, Series: Medicine and Biology Pub Date : 2022-11-24 DOI: 10.22190/fumb220819006z

D. Živanović, Z. Marjanovic

引用次数: 0

AGRYPON POLYXENAE (SZÉPLIGETI, 1899) (HYMENOPTERA: ICHNEUMONIDAE: ANOMALONINAE); NEWLY RECORDED PARASITOID OF ZERYNTHIA POLYXENA (DENIS & SCHIFFERMÜLLER, 1775) (LEPIDOPTERA: PAPILIONIDAE: PARNASSIINAE) IN THE FAUNA OF SERBIA

Facta Universitatis, Series: Medicine and Biology Pub Date : 2022-10-01 DOI: 10.22190/fumb220303004z

V. Žikić, Saša S Stanković, M. Popović, Aleksandra Husarik, Marijana Ilić Milošević, Maja Lazarević, Aleksandra Trajković

引用次数: 0