Journal of Pharmaceutical Research最新文献_第6页

Phytochemical Evaluation of Ficus Carica Seeds and their Cold Pressed Oil 无花果种子及其冷榨油的植物化学评价

Journal of Pharmaceutical Research Pub Date : 2021-12-15 DOI: 10.18579/jopcr/v20i4.2

Zeliha Ustun-Argon, Z. Sarı, A. Gokyer, Sidika Buyukhelvacigil-Ozturk

{"title":"Phytochemical Evaluation of Ficus Carica Seeds and their Cold Pressed Oil","authors":"Zeliha Ustun-Argon, Z. Sarı, A. Gokyer, Sidika Buyukhelvacigil-Ozturk","doi":"10.18579/jopcr/v20i4.2","DOIUrl":"https://doi.org/10.18579/jopcr/v20i4.2","url":null,"abstract":"The use of plant materials as the source of oil and functional foods is becoming more important with the new trends and scientific research. In this study, fig (Ficus carica) seed, which is a rarely found, seed and its oil, have been studied to determine the phytochemical properties for possible applications. Fig seeds were obtained from Aydın, Turkey. The oil was extracted by the cold press method. The seeds were analyzed for oil yield, protein, ash, ash insoluble in hydrochloric acid and trace elements. The oil was evaluated for fatty acid, sterol compositions by GC and the volatiles were analyzed with GCMS, tocopherols analyzed by HPLC, total phenolic content and antioxidant activity were determined spectrophotometrically. The oil was also evaluated for free fatty acid (FFA), refractive index (RI), peroxide (PV) and p-anisidine value. The results showed that F. carica has 22.7% oil yield and was characterized by linolenic (41.27%), linoleic (30.06%), and oleic acids (18.10%). Total phenolics were 79.5 mg GAE/100 g, and nine different sterol components were analyzed and β-sitosterol was at the highest rate. Theα-β-γ-δ tocopherol content was 6.088, 0.18, 634, 18 mgkg-1, respectively. Antioxidant capacity was 52.54%. Fenchone, cymene, styrene d-limonene, linalool, camphor were found as important volatiles. Quality parameters were FFA (% oleic acid) 0.90, RI (40 oC) 1.4721, PV (meq O2/kg oil) 2.2, p-anisidine 2.22. As a result, F. carica oil can be an alternative oil source and a good option for functional foods with their rich contentof phytochemicals. For the future, more studies need to be done for both the seeds and the oil for further applications. Keywords Ficus carica seed, cold-pressed oil, fig seed, phytochemical properties","PeriodicalId":16706,"journal":{"name":"Journal of Pharmaceutical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46800422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Validation of Novel RP-HPLC Method for the Estimation of Naloxegol in Pharmaceutical Dosage Forms 新型反相高效液相色谱法测定药物剂型中纳洛格尔含量的验证

Journal of Pharmaceutical Research Pub Date : 2021-12-15 DOI: 10.18579/jopcr/v20i4.suneetha

A. Suneetha, G. Priyadarshini, V. Mounika, A. Ameen, K. Jyothi, A. Babitha

{"title":"Validation of Novel RP-HPLC Method for the Estimation of Naloxegol in Pharmaceutical Dosage Forms","authors":"A. Suneetha, G. Priyadarshini, V. Mounika, A. Ameen, K. Jyothi, A. Babitha","doi":"10.18579/jopcr/v20i4.suneetha","DOIUrl":"https://doi.org/10.18579/jopcr/v20i4.suneetha","url":null,"abstract":"Background: Naloxegol is a peripherally acting µ-opioid antagonist. Purpose: The aim of the present research was to develop and validate a Reverse Phase High-Performance Liquid Chromatography for quantitative determination of Naloxegol in pharmaceutical dosage forms. Methodology: HPLC system used was Shimadzu coupled to a Photodiode Array Detector and was operated in an isocratic mode. Separation was achieved using Inertsil-C18 ODS column having dimensions 250 mm × 4.6 mm, 5 μm and the mobile phase composed of 90 volumes of methanol and 10 volumes of acetonitrile mixture. The flow rate of the mobile phase was 1 mL min−1. Detection wavelength was 250 nm and temperature was 25°C. Findings: The method was validated with regard to linearity, accuracy, precision, selectivity, and robustness in accordance with ICH guidelines. Conclusion: From this study it was concluded that the proposed method is accurate, reproducible and precise. Application: The method was applied successfully for the estimation of Naloxegol in marketed tablet dosage form. Keywords High-Performance Liquid Chromatography, Naloxegol","PeriodicalId":16706,"journal":{"name":"Journal of Pharmaceutical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43624707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LC–MS/MS Determination of Prednisone, A Drug in Phase 4 of Multiple Sclerosis Therapy along with Teriflunomide and Pioglitazone in Rat Plasma LC–MS/MS法测定大鼠血浆中多发性硬化症4期药物泼尼松与特立氟胺和吡格列酮的浓度

Journal of Pharmaceutical Research Pub Date : 2021-12-15 DOI: 10.18579/jopcr/v20i4.ms21076

R. Rajeswari, A. Suneetha

{"title":"LC–MS/MS Determination of Prednisone, A Drug in Phase 4 of Multiple Sclerosis Therapy along with Teriflunomide and Pioglitazone in Rat Plasma","authors":"R. Rajeswari, A. Suneetha","doi":"10.18579/jopcr/v20i4.ms21076","DOIUrl":"https://doi.org/10.18579/jopcr/v20i4.ms21076","url":null,"abstract":"Combination therapy or polytherapy is that uses more than one medication to treat a single disease and associated diseases. Pharmaceutical combination therapy may be achieved by prescribing separate drugs, or, where available, dosage forms that contain more than one active ingredient. A randomized efficacy phase 4 study is going on for prednisone in the treatment of multiple sclerosis, which is a neurodegenerative disease associated with many other disorders. A novel and accurate liquid chromatography tandem mass spectrometry method using electrospray ionization mode has been developed and validated for the simultaneous determination of prednisone (PDN), pioglitazone (PGZ) and teriflunomide (TFM) using imipramine (IMP) and ibuprofen (IBP) as internal standards (IS). The separation was carried on XTerra MS C18 (100 mm x 3.9 mm, 5 µm) reversed phase column using acetonitrile and 0.01M ammonium formate as the mobile phase in gradient mode at 1.0 mL/min. The method was validated in terms of specificity, linearity, accuracy and precision over the concentration range of 1–500 ng/mL. The intra and inter-day precision and accuracy, stability and extraction recoveries of all the analytes were found in the range of 97.2-102.2%. The lower limit of quantitation was 1.0 ng/mL for all the 3 analytes and the extraction recovery values were more than 65%. The method proved highly reproducible and sensitive and was successfully applied to pharmacokinetic study after single dose oral administration of PDN, PGZ and TFM to the rats. Keywords Polytherapy, Teriflunomide, Prednisone, Pioglitazone, Phase 4, Multiple Sclerosis, Pharmacokinetics","PeriodicalId":16706,"journal":{"name":"Journal of Pharmaceutical Research","volume":" 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41252661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solvent Extraction and Phytochemical Screening of Seeds, Coats, Pods and Leaves of Moringa Plant 辣木种子、皮、荚和叶的溶剂提取及植物化学筛选

Journal of Pharmaceutical Research Pub Date : 2021-11-10 DOI: 10.33140/jpr.06.02.06

{"title":"Solvent Extraction and Phytochemical Screening of Seeds, Coats, Pods and Leaves of Moringa Plant","authors":"","doi":"10.33140/jpr.06.02.06","DOIUrl":"https://doi.org/10.33140/jpr.06.02.06","url":null,"abstract":"The potency of six different solvents in extracting phytochemicals from the seeds, coats, pods and leaves of moringa plant was investigated. The seeds, coats, pods and leaves of the plant were cut into smaller pieces, air-dried, ground into powdery sample, sieved with 40 mm mesh size and properly labelled. Each sample was individually extracted using six different solvents (methanol, ethanol, chloroform, ethyl acetate, water and acetone) at ratio 1: 10 for 72 h. Each solvent extract was screened for twelve phytochemicals (alkaloid, flavonoid, saponin, cardiac glycoside, reducing sugar, tannin, quinone, volatile oil, phenol, terpenoid, phlobatannin and steroid). It was observed that the seeds and leaves of moringa plant were richest in phytochemicals followed by moringa pods and the least was in moringa coat. In all the six solvents used, thirty-four bioactive ingredients were detected in seeds and leaves of moringa plant while twenty-eight phytochemicals were obtained in moringa pods and twenty-one bioactive ingredients were gotten from moringa coats. In all the plant samples, twenty-three bioactive ingredient were detected in ethanol extract; twenty-one were obtained in each of acetone, ethyl acetate and methanol extracts; water extract had sixteen phytochemicals and chloroform extract had fifteen bioactive ingredients. Among the solvents used for extraction for all the plant samples, ethanol ranked first while acetone, ethyl acetate and methanol ranked second, water ranked third and chloroform was the least in ranking.","PeriodicalId":16706,"journal":{"name":"Journal of Pharmaceutical Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69508758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Relationship of Amniotic Fluid Index (AFI) Between Maternal and Perinatal in Patients 羊水指数（AFI）与产妇及围产期的关系

Journal of Pharmaceutical Research Pub Date : 2021-09-20 DOI: 10.9734/JPRI/2021/V33I44A32638

Neranjana Manimekalai

引用次数: 0

Simultaneous Estimation of Protein and Nucleic Acid Using Derivative Spectrophotometric Method 导数分光光度法同时测定蛋白质和核酸

Journal of Pharmaceutical Research Pub Date : 2021-09-15 DOI: 10.18579/jopcr/v20i3.ms21041

A. Agrawal, K. Kaur, Ra Sharma, Amit Goyal, A. Thakkar

{"title":"Simultaneous Estimation of Protein and Nucleic Acid Using Derivative Spectrophotometric Method","authors":"A. Agrawal, K. Kaur, Ra Sharma, Amit Goyal, A. Thakkar","doi":"10.18579/jopcr/v20i3.ms21041","DOIUrl":"https://doi.org/10.18579/jopcr/v20i3.ms21041","url":null,"abstract":"Purpose: Simple and precise first derivative zero crossing spectrophotometric method has been developed for the simultaneous estimation of protein and nucleic acid without prior separation. Approach: The concentration ranges for protein and nucleic acid were taken in the range of 60-140 m g mL -1 and 20-100 m g mL -1 respectively in 0.1 M NaOH. Absorption spectra of the samples were recorded between 200 nm to 400 nm against a reagent blank. Zero-order spectra of protein and nucleic acid were stored individually within the above concentration ranges and were derivatized in first order using scaling factor 5 for both the substances. First derivative amplitudes were recorded at 261 nm and 289 nm for estimation of protein and RNA respectively for pure substances and binary mixture. Finding: Limit of detection was found to be 2.90 m g mL -1 and 0.36 m g mL -1 and limit of quantitation was 8.80 m g mL -1 and 1.02 m g mL -1 respectively for protein and nucleic acid respectively. Precision was found to be 1.38 % for protein and 1.25 % for RNA. Reproducibility was found to be 1.54 % for protein and 1.69 % for RNA. Conclusions: Thus, proposed method can be adapted for simultaneous determination of protein and nucleic acid and better alternate technique for immunoassays and electrophoretic methods.","PeriodicalId":16706,"journal":{"name":"Journal of Pharmaceutical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47603542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development and Validation of RP-HPLC Method for the Estimation of Dolutegravir and Rilpivirine in Bulk and its Tablet Dosage form RP-HPLC法测定多卢替拉韦和利匹韦林的含量及片剂剂型的建立与验证

Journal of Pharmaceutical Research Pub Date : 2021-09-15 DOI: 10.18579/jopcr/v20i3.ms21040c

P. Pravalika, G. T. Rani, P. T. Sree, Y. Saritha

引用次数: 0

Comparative In Vitro Quality Evaluation of Brands of Propranolol Tablet Marketed in Okada, Edo State, Nigeria. 尼日利亚江户州Okada市售品牌心得安片体外质量比较评价

Journal of Pharmaceutical Research Pub Date : 2021-09-15 DOI: 10.18579/jopcr/v20i3.ms21055

Oluwatobi O Olakojo, Precious Okpara, Stephanie Ikebiagbo

{"title":"Comparative In Vitro Quality Evaluation of Brands of Propranolol Tablet Marketed in Okada, Edo State, Nigeria.","authors":"Oluwatobi O Olakojo, Precious Okpara, Stephanie Ikebiagbo","doi":"10.18579/jopcr/v20i3.ms21055","DOIUrl":"https://doi.org/10.18579/jopcr/v20i3.ms21055","url":null,"abstract":"Propranolol, a beta-blocker is used in the management of cardiovascular conditions such as irregular heart rate and high blood pressure.The study was carried out to examine the in vitro quality control tests for seven brands of propranolol hydrochloride 40mg tablet formulation, sold in retail pharmacies in Okada, EdoState, Nigeria. The parameters determined were identification, weight variation, friability, hardness, disintegration, dissolution rate, and assay of the tablets. The tablets were evaluated for conformity with British Pharmacopoeia (BP) standards. Results obtained showed tablet weight in the range of 155.6±3.2mg to 348.2±2.0mg, hardness ranged from 1.03±0.17to 10.70±0.90 kg/cm2, friability of < 1 % except for one brand, disintegration time of 1.37±0.15 to 18.05±2.88 min whereby two brands are uncoated tablets and assay of 90.07 ±1.15 to 102±1.62% with one brand deviating from the specified limit. The seven batches also released more than 80% of their drug content within 30min. Analysis of similarity factor revealed that all brands but PN-7 can be interchangeable with PN-1 in terms of dissolution profile. The study showed that propranolol samples examined passed all the Pharmacopoeial tests for satisfactory quality exceptPN-6 which did not comply with most of the Pharmacopoeial specifications. Thus, not all brands can be used interchangeably in clinical practice.","PeriodicalId":16706,"journal":{"name":"Journal of Pharmaceutical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48232633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Profiling and Antihyperglycaemic Study on Butanol Fraction of Chlorophytum alismifolium Baker (Liliaceae) 百合科吊兰丁醇组分的化学谱分析及降糖作用研究

Journal of Pharmaceutical Research Pub Date : 2021-09-15 DOI: 10.18579/jopcr/v20i3.cp

Abubakar Abdulhakim, Omogbai E K Inanemo, Nazifi A Balarabe, Sania Bashir

{"title":"Chemical Profiling and Antihyperglycaemic Study on Butanol Fraction of Chlorophytum alismifolium Baker (Liliaceae)","authors":"Abubakar Abdulhakim, Omogbai E K Inanemo, Nazifi A Balarabe, Sania Bashir","doi":"10.18579/jopcr/v20i3.cp","DOIUrl":"https://doi.org/10.18579/jopcr/v20i3.cp","url":null,"abstract":"Purpose: Diabetes mellitus is a disorder associated with debilitating complications. This study was aimed at evaluating the chemical profile and antihyperglycaemic effect of butanol fraction of Chlorophytum alismifolium. Methodology: The powdered plant was extracted sequentially using soxhlet apparatus with solvents of varying polarities until butanol fraction was obtained. GC-MS analysis, phytochemical screening and acute toxicity studies were carried out. Antihyperglycaemic study was carried out using alloxaninduced hyperglycaemia in rats. Male Wistar rats were injected with 120 mg/kg of alloxan intraperitoneally, the rats with fasting blood glucose levels between 200 and 350 mg/dL were considered hyperglycaemic. Experimental groups were set up using normal rats in group I and hyperglycaemic rats in five groups of six rats each. Group II was the hyperglycaemic control while groups III, IV and V received the butanol fraction of C. alismifolium at 250, 500 and 1000 mg/kg respectively. Group VI received glimepiride 1 mg/kg. Blood glucose levels were monitored before treatment at 0 hour and 1, 2, 3 and 5 hours after treatment. Findings: Phytochemical screening revealed the presence of alkaloids, saponins, flavonoids, glycosides and triterpenes while GC-MS analysis revealed the presence of thirteen compounds some of which include; isoxazolidine, isothiazole and acetamide. Oral median lethal dose of the extract in rats was estimated to be >5,000 mg/kg. The butanol fraction of C. alismifolium at all the doses tested showed significant (p<0.05) blood glucose lowering effect when compared over time.Conclusion: Thefindings from this research showed that butanol fraction of Chlorophytum alismifolium possesses important compounds with antihyperglycaemic activity.","PeriodicalId":16706,"journal":{"name":"Journal of Pharmaceutical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47243519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and Characterization of Buccal Films of Benzocaine for Mouth Ulcer 苯佐卡因口腔溃疡膜的设计与表征

Journal of Pharmaceutical Research Pub Date : 2021-09-15 DOI: 10.18579/jopcr/v20i3.ms21062

J. Jose, M. V. Sisira, M. Prasanth, C. Prasanth, P. Pradeep

{"title":"Design and Characterization of Buccal Films of Benzocaine for Mouth Ulcer","authors":"J. Jose, M. V. Sisira, M. Prasanth, C. Prasanth, P. Pradeep","doi":"10.18579/jopcr/v20i3.ms21062","DOIUrl":"https://doi.org/10.18579/jopcr/v20i3.ms21062","url":null,"abstract":"Mouth ulcer is very common in recent years, which occurs due to the damage of epithelial tissue and/or lamina propria that finally leads to tissue necrosis. Benzocaine has been used to treat the mouth ulcers due to its excellent local anaesthetic effect that relieves the pain associated with mouth ulcer. Hence an attempt was made to develop and characterize the buccal films of Benzocaine to treat mouth ulcers with an aim of prolonging the drug release and improving the patient convenience. The films were fabricated using the mucoadhesivepolymerblendofchitosanandHPMCbysolventcastingmethodandthephysico-mechanical, in vitro drug release and ex vivo buccal mucosal permeation characteristics of the films were studied. All fabricated film formulations prepared were smooth and almost opaque, with good flexibility. The weight and thickness of all the formulations were found to be uniform. Drug content in the films ranged from 97–99%, indicating favorable drug loading and uniformity. The inclusion of HPMC, significantly reduced the bioadhesive strength and in vitro mucoadhesion time of chitosan films, although the degree of swelling increased. In vitro drug release and permeation studies in simulated saliva showed a prolonged release for a period of 6 h for all formulations. The formulation with Chitosan: HPMC ratio 1:1, 10% w/w polysorbate 80 and 10% w/w propylene glycol as plasticizers showed the best results which exhibited the cumulative percentagedrugreleaseof87.9%andthecumulativeamountofdrugpermeationof7.62mg/cm 2 acrossgoat buccal mucosa in 6 h. Drug-excipient interaction studies were carried out using DSC and FT-IR technique; films indicated no chemical interaction between drug and polymers used.","PeriodicalId":16706,"journal":{"name":"Journal of Pharmaceutical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46654900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0