Journal of pharmaceutical and biomedical analysis最新文献

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Identification of TRAF2, CAMK2G, and TIMM17A as biomarkers distinguishing mechanical asphyxia from sudden cardiac death base on 4D-DIA Proteomics: A pilot study
IF 3.1 3区 医学
Journal of pharmaceutical and biomedical analysis Pub Date : 2025-02-03 DOI: 10.1016/j.jpba.2025.116730
Yuebing Huang, Hai Qiu, Wen Chen, Zilin Meng, Yu Cai, Dongfang Qiao, Xia Yue
{"title":"Identification of TRAF2, CAMK2G, and TIMM17A as biomarkers distinguishing mechanical asphyxia from sudden cardiac death base on 4D-DIA Proteomics: A pilot study","authors":"Yuebing Huang,&nbsp;Hai Qiu,&nbsp;Wen Chen,&nbsp;Zilin Meng,&nbsp;Yu Cai,&nbsp;Dongfang Qiao,&nbsp;Xia Yue","doi":"10.1016/j.jpba.2025.116730","DOIUrl":"10.1016/j.jpba.2025.116730","url":null,"abstract":"<div><div>In the context of forensic medicine, the differential diagnosis between mechanical asphyxia and sudden cardiac death is very important regarding the establishment of the cause of death. Traditional autopsy findings have generally been very nonspecific; accordingly, highlighting the need for more specific molecular biomarkers. This study employed four-dimensional data-independent acquisition (4D-DIA) proteomics technology, in combination with both animal models and human samples, to conduct a comprehensive protein expression analysis of cardiac tissues, identifying 7557 proteins, among which 142 shared differentially expressed proteins (DEPS) were screened out. Based on the protein interaction network and through rigorous screening, this study identified three proteins, namely TNF receptor-associated factor 2 (TRAF2), Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G), and translocase of inner mitochondrial membrane 17 homolog A (TIMM17A), as biomarkers for differentiating mechanical asphyxia from sudden cardiac death. Further verification using Western Blot (WB) and immunohistochemistry (IHC) proved the differential expression of these biomarkers in both animal and human samples. These findings, besides deepening the molecular understanding of the pathophysiological differences between sudden cardiac death and mechanical asphyxia, also provided new biomarkers for forensic applications that could enable the improvement of accuracy and reliability in the determination of the cause of death.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"258 ","pages":"Article 116730"},"PeriodicalIF":3.1,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143289645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nanomaterial-based electrochemical sensors for anti-HIV drug monitoring: Innovations, challenges, and prospects
IF 3.1 3区 医学
Journal of pharmaceutical and biomedical analysis Pub Date : 2025-02-03 DOI: 10.1016/j.jpba.2025.116727
Abdellatif Ait Lahcen, Gymama Slaughter
{"title":"Nanomaterial-based electrochemical sensors for anti-HIV drug monitoring: Innovations, challenges, and prospects","authors":"Abdellatif Ait Lahcen,&nbsp;Gymama Slaughter","doi":"10.1016/j.jpba.2025.116727","DOIUrl":"10.1016/j.jpba.2025.116727","url":null,"abstract":"<div><div>Monitoring human immunodeficiency virus (HIV) and anti-HIV drugs is critical for optimizing treatment outcomes and preventing drug resistance. Accurate detection and quantification of anti-HIV drugs are essential to ensure appropriate dosing, enhancing patient care and therapeutic efficacy. Electrochemical biosensors have emerged as a pivotal tool in this context, offering high sensitivity, specificity, and rapid response times. Leveraging advancements in nanomaterials, these sensors provide reliable and efficient solutions for point-of-care (POC) applications in clinical and environmental settings. This review presents a comprehensive analysis of recent innovations in electrochemical sensor technologies for anti-HIV drug detection and quantification, focusing on nanomaterial-based platforms. It addresses the challenges of developing and implementing these technologies, including matrix effects, stability, and scalability. Furthermore, the review explores future directions, emphasizing the integration of sensors into POC systems and their potential to revolutionize personalized HIV treatment and pharmaceutical monitoring.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"258 ","pages":"Article 116727"},"PeriodicalIF":3.1,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inter-individual variability in the metabolism of psychotropic drugs by the enzyme activities from the human gut microbiome
IF 3.1 3区 医学
Journal of pharmaceutical and biomedical analysis Pub Date : 2025-02-01 DOI: 10.1016/j.jpba.2025.116717
Xuan Wang , Yuanfeng Lyu , Sau Wan Cheng , Tsz Fung Tsang , Ka Chun Chong , Siaw Shi Boon , Xiao Yang , Christopher KC Lai , Paul KS Chan , Zhong Zuo
{"title":"Inter-individual variability in the metabolism of psychotropic drugs by the enzyme activities from the human gut microbiome","authors":"Xuan Wang ,&nbsp;Yuanfeng Lyu ,&nbsp;Sau Wan Cheng ,&nbsp;Tsz Fung Tsang ,&nbsp;Ka Chun Chong ,&nbsp;Siaw Shi Boon ,&nbsp;Xiao Yang ,&nbsp;Christopher KC Lai ,&nbsp;Paul KS Chan ,&nbsp;Zhong Zuo","doi":"10.1016/j.jpba.2025.116717","DOIUrl":"10.1016/j.jpba.2025.116717","url":null,"abstract":"<div><div>The interaction between marketed drugs and the gut microbiome is increasingly being recognized, and it is now known that enzymes produced by the bacteria in the human gut can degrade psychotropic drugs. However, the degree of inter-individual variation in their metabolism remains largely unknown. Here, we present a simple model for detecting individual drug-microbiome interaction using fecalase. We incubated fecalase prepared from freshly collected stool samples from healthy volunteers (n = 18) and incubated with nine selected psychotropic medications. We proved fecalase retained enzymatic activities and showed the degree of drug degradation differed significantly for different psychotropic drugs, and there was significant inter-individual variation in the metabolism of phenytoin, amitriptyline, and chlorpromazine with the inter-individual point difference between the strongest and weakest metabolizer of 85 %, 39 %, and 30 % respectively. These findings highlight the potential of fecalase as a model for studying personalized drug metabolism and underscore the importance of considering gut microbiome variability in pharmacological research.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"258 ","pages":"Article 116717"},"PeriodicalIF":3.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143352318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
UHPLC-MS/MS-based analysis of 17 nitazenes in human hair for practical forensic casework with simultaneous separation of 6 groups of isomers
IF 3.1 3区 医学
Journal of pharmaceutical and biomedical analysis Pub Date : 2025-01-31 DOI: 10.1016/j.jpba.2025.116707
Guoqing Gao , Shuo Yang , Xin Wang , Ping Xiang , Liting Ma , Fang Yan , Yan Shi
{"title":"UHPLC-MS/MS-based analysis of 17 nitazenes in human hair for practical forensic casework with simultaneous separation of 6 groups of isomers","authors":"Guoqing Gao ,&nbsp;Shuo Yang ,&nbsp;Xin Wang ,&nbsp;Ping Xiang ,&nbsp;Liting Ma ,&nbsp;Fang Yan ,&nbsp;Yan Shi","doi":"10.1016/j.jpba.2025.116707","DOIUrl":"10.1016/j.jpba.2025.116707","url":null,"abstract":"<div><div>Nitazenes, a new class of potent synthetic opioids characterized by a 2-benzyl-benzimidazole structural core, have emerged on the illicit drug market following fentanyl. Compared to traditional opioids, nitazenes pose heightened risks of respiratory depression and central nervous system suppression. Due to the ease of modification of its parent nucleus, abusers and dealers can rapidly synthesize new structural analogs that fall outside the drug control catalog. Consequently, multiple isomers of nitazenes have emerged, causing significant interference in forensic practices. As an increasing number of poisoning and fatality cases were associated with nitazenes, there is an urgent need for developing an analytical method that can effectively address the challenges currently encountered in their identification in forensic practices. In this study, we established and validated a simple and high-efficiency ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for detecting 17 nitazenes in human hair. All analytes were separated in 6.5 min on a biphenyl column with simultaneous separation of six groups of isomers. Method validation demonstrated linear calibration over the range of 5–1000 pg/mg (r &gt; 0.995), with limits of detection (LODs) ranging from 1 to 15 pg/mg and limits of quantification (LOQs) ranging from 5 to 20 pg/mg. The method also exhibited precision (&lt;15 %) and accuracy ( ± 15 %), along with excellent recovery, acceptable matrix effects, and good stability at 4 ℃ for at least 72 h. When applied to authentic cases, this method successfully identified two positive hair samples and N,N-dimethylamino etonitazene was detected at concentrations of 1528.5 pg/mg and 463.9 pg/mg<sup>,</sup> respectively. This method provides technical support for the rapid and accurate detection of nitazenes, better meeting the needs of anti-drug operations and forensic practice.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"257 ","pages":"Article 116707"},"PeriodicalIF":3.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143161411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Salting-out assisted liquid-liquid extraction (SALLE): Principle, optimization, and applications in blood sample analysis
IF 3.1 3区 医学
Journal of pharmaceutical and biomedical analysis Pub Date : 2025-01-31 DOI: 10.1016/j.jpba.2025.116720
Jean Simon Thodhekes Yao , Yoann Ladner , N.’Cho Christophe Amin , Catherine Perrin
{"title":"Salting-out assisted liquid-liquid extraction (SALLE): Principle, optimization, and applications in blood sample analysis","authors":"Jean Simon Thodhekes Yao ,&nbsp;Yoann Ladner ,&nbsp;N.’Cho Christophe Amin ,&nbsp;Catherine Perrin","doi":"10.1016/j.jpba.2025.116720","DOIUrl":"10.1016/j.jpba.2025.116720","url":null,"abstract":"<div><div>This review focuses on the use of Salting-Out Assisted Liquid-Liquid Extraction (SALLE) for blood sample processing prior analysis, exploring its principles, optimization parameters, and coupling with analytical techniques. SALLE is favored for its simplicity, cost-effectiveness, and rapid execution using the salting-out effect to induce phase separation in mixtures of aqueous samples and water-miscible organic solvents. The review categorizes and discusses the different types of blood samples, the main salts and solvents used, and the parameters affecting the extraction efficiency, such as solvent and salt type as well as ionic strength, pH, vortex mixing and centrifugation time. Advantages of SALLE over traditional methods like solid-phase extraction (SPE) and protein precipitation (PP) are highlighted, emphasizing its environmental friendliness, high extraction efficiency, and ease of automation. By examining recent literature and scientific publications (2014–2024), this review provides a comprehensive understanding of the factors influencing SALLE performance and its application in bioanalysis, particularly when coupled with separative techniques such as liquid chromatography (LC), capillary electrophoresis (CE), and mass spectrometry (MS).</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"257 ","pages":"Article 116720"},"PeriodicalIF":3.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MALDI MSI-based spatial amine metabolomics revealing the protective effect of combination therapy against cerebral ischemia/reperfusion-induced brain injury in rats
IF 3.1 3区 医学
Journal of pharmaceutical and biomedical analysis Pub Date : 2025-01-31 DOI: 10.1016/j.jpba.2025.116715
Kening Li , Siyu Wang , Weiwei Tang , Yanwen Chen , Bin Li
{"title":"MALDI MSI-based spatial amine metabolomics revealing the protective effect of combination therapy against cerebral ischemia/reperfusion-induced brain injury in rats","authors":"Kening Li ,&nbsp;Siyu Wang ,&nbsp;Weiwei Tang ,&nbsp;Yanwen Chen ,&nbsp;Bin Li","doi":"10.1016/j.jpba.2025.116715","DOIUrl":"10.1016/j.jpba.2025.116715","url":null,"abstract":"<div><div>Complex amine metabolic disorders are implicated in ischemic stroke and can further exacerbate brain damage. Therefore, ameliorating their metabolic disorder would be an effective way to improve recovery from brain damage after ischemia/reperfusion (I/R) injury. In this work, the protective effects of Edaravone (Eda), Ginaton injection (Gin), and their combination (Eda+Gin) against cerebral I/R injury in a middle cerebral artery occlusion/reperfusion (MCAO/R) rat model were preliminarily investigated from the perspective of spatial metabolomics. Compared to single-drug treatment, the optimized combination treatment with Eda and Gin significantly decreased infarct volumes, improved neurological function, and inhibited neuronal damage and glial cell activation in MCAO/R rats. Also, combination treatment could prolong the blood circulation time of quercetin, ginkgolide C, and eight flavonoid glycosides compared to Gin treatment alone. More importantly, the spatial metabolic alterations of amine metabolites in MCAO/R rats before and after drug treatment were comprehensively interrogated using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) coupled with laser-assisted chemical transfer (LACT)-based on tissue chemical derivatization, such as amino acids, dipeptides, tripeptides, neurotransmitters, and the other amine metabolites. MALDI MSI results showed that the drastic metabolic disorders occurred in the cerebral cortex (CTX) and striatum (STR) and combination treatment exerted a better regulatory effect on the perturbed spatial amine metabolism. Therefore, combination treatment with Eda and Gin could significantly reduce ischemic brain damage and correct spatial metabolic disorders of amine metabolites, providing a potential treatment strategy for cerebral I/R injury.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"257 ","pages":"Article 116715"},"PeriodicalIF":3.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143162178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Simultaneous quantification of eighteen therapeutic oral anticoagulants, rodenticides, and antiplatelet agents by LC-MS/MS and its application in post-mortem forensic cases
IF 3.1 3区 医学
Journal of pharmaceutical and biomedical analysis Pub Date : 2025-01-31 DOI: 10.1016/j.jpba.2025.116709
Angélique Drague , Jean Escal , Carolyne Bidat , Sandrine Dellinger , Sophie Hodin , Sébastien Duband , Catherine Feliu , Xavier Delavenne
{"title":"Simultaneous quantification of eighteen therapeutic oral anticoagulants, rodenticides, and antiplatelet agents by LC-MS/MS and its application in post-mortem forensic cases","authors":"Angélique Drague ,&nbsp;Jean Escal ,&nbsp;Carolyne Bidat ,&nbsp;Sandrine Dellinger ,&nbsp;Sophie Hodin ,&nbsp;Sébastien Duband ,&nbsp;Catherine Feliu ,&nbsp;Xavier Delavenne","doi":"10.1016/j.jpba.2025.116709","DOIUrl":"10.1016/j.jpba.2025.116709","url":null,"abstract":"<div><div>Anticoagulants and antiplatelet agents, which interfere with blood coagulation, can lead to fatal bleeding. Moreover, their widespread use as drugs or rodenticides poses a high risk of accidental or intentional poisoning. Therefore, quantifying these substances is essential in forensic investigations. This research aimed to develop and validate a liquid chromatography-tandem mass spectrometry method capable of quantifying eighteen anticoagulant or antiplatelet compounds (apixaban, rivaroxaban, dabigatran, warfarin, acenocoumarol, fluindione, brodifacoum, bromadiolone, difenacoum, difethialone, chlorophacinone, coumatetralyl, flocoumafen, acetylsalicylic acid, clopidogrel, dipyridamole, ticagrelor, and ticlopidine) in a single run with simple sample preparation. The method was validated according to the FDA recommendations for all compounds, with an eight-min run time in human whole blood, the gold standard in toxicological forensic investigation. The method was also validated for therapeutic compounds and most rodenticides in bile and vitreous humor. Following validation, the method was applied to seven forensic cases with a known history of anticoagulant or antiplatelet agent use to prove the validity of the method. This method provides a valuable tool for legal contexts where precise determination of anticoagulant compound presence and concentration is required.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"257 ","pages":"Article 116709"},"PeriodicalIF":3.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A preliminary study on estimation of postmortem submersion interval of rat cadavers in freshwater through polyamine analysis in tissues
IF 3.1 3区 医学
Journal of pharmaceutical and biomedical analysis Pub Date : 2025-01-31 DOI: 10.1016/j.jpba.2025.116706
Pei Zhang , Wei Xia , Yang Bai , Fuyuan Zhang , Yan Zhang , Wei Jiang , Huiya Yuan
{"title":"A preliminary study on estimation of postmortem submersion interval of rat cadavers in freshwater through polyamine analysis in tissues","authors":"Pei Zhang ,&nbsp;Wei Xia ,&nbsp;Yang Bai ,&nbsp;Fuyuan Zhang ,&nbsp;Yan Zhang ,&nbsp;Wei Jiang ,&nbsp;Huiya Yuan","doi":"10.1016/j.jpba.2025.116706","DOIUrl":"10.1016/j.jpba.2025.116706","url":null,"abstract":"<div><div>The estimation of postmortem submersion interval (PMSI) has always been an important scientific issue to be addressed in drowning cases. Traditional methods, such as corpse temperature analysis and the assessment of corpse surface corruption, have limitations and cannot meet the need for accurate estimation of the time of death in the mid to late stages. Biogenic amines, as small molecules produced by protein degradation after death, have a certain regularity in relation to PMSI. To further explore the possibility of utilizing polyamines to estimate PMSI, this experiment constructed a rat cadaver model in both laboratory constant-temperature water and natural water bodies. Furthermore, cadaverine and putrescine in the liver and skeletal muscle were detected at different PMSI using gas chromatography-mass spectrometry (GC-MS). Through statistical analysis, we have constructed eight sets of mathematical models for polyamines-PMSI estimation, and determined the applicable time range through derivative analysis. After evaluation the models, the error rate in inferring PMSI using the fitted equations was less than 30 % within 242 h. The models established in this study could accurately infer PMSI in the mid to late stages of the postmortem period, providing a feasible approach for the drowning forensic issue.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"257 ","pages":"Article 116706"},"PeriodicalIF":3.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143161412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
LC-MS/MS method for the simultaneous quantification of thiafentanil and naltrexone in bovine muscle, liver and kidney
IF 3.1 3区 医学
Journal of pharmaceutical and biomedical analysis Pub Date : 2025-01-30 DOI: 10.1016/j.jpba.2025.116711
Judith T. Christie , Mieghan Bruce , Silke Pfitzer , Liesel Laubscher , Jacobus P. Raath , Michael Laurence , Tracy Kellermann
{"title":"LC-MS/MS method for the simultaneous quantification of thiafentanil and naltrexone in bovine muscle, liver and kidney","authors":"Judith T. Christie ,&nbsp;Mieghan Bruce ,&nbsp;Silke Pfitzer ,&nbsp;Liesel Laubscher ,&nbsp;Jacobus P. Raath ,&nbsp;Michael Laurence ,&nbsp;Tracy Kellermann","doi":"10.1016/j.jpba.2025.116711","DOIUrl":"10.1016/j.jpba.2025.116711","url":null,"abstract":"<div><div>Thiafentanil is a µ-opioid agonist used for the chemical immobilisation of a variety of ungulate species and is antagonised by the administration of naltrexone. The potential for these ungulates to be hunted for consumption by humans or predators raises concerns of drug residues in animal tissues. No analytical method to quantify tissue residue concentrations of thiafentanil has been previously reported. This research developed an LC-MS/MS method to quantify thiafentanil in bovine muscle, and both thiafentanil and naltrexone in bovine liver and kidney matrices. The analytical method was applied to quantify tissue residues in samples collected from goats 1, 2, 3, and 6 days post thiafentanil administration. The assay was validated over the calibration range 6.25–200 ng/mg for thiafentanil in muscle, and 3.13–400 ng/mg for thiafentanil and 57.8–7400 ng/mg for naltrexone in liver and kidney. No residues above the lowest limit of quantification were detected in the injection site, <em>longissimus dorsi</em> muscle, liver or kidney samples collected from the goats. The reported analytical method and residue depletion data provide a foundation for future thiafentanil and naltrexone residue depletion studies in wildlife species.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"257 ","pages":"Article 116711"},"PeriodicalIF":3.1,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143161410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The nonanol/surfactant directed self-assembly supramoleculars heat-shrinkable tubing liquid phase microextraction for determination of flavonoids in natural products combined with high performance liquid chromatography
IF 3.1 3区 医学
Journal of pharmaceutical and biomedical analysis Pub Date : 2025-01-30 DOI: 10.1016/j.jpba.2025.116704
Jie Li , Weizheng Fu , Yuzhen Zhang , Shuang Hu , Xuan Chen
{"title":"The nonanol/surfactant directed self-assembly supramoleculars heat-shrinkable tubing liquid phase microextraction for determination of flavonoids in natural products combined with high performance liquid chromatography","authors":"Jie Li ,&nbsp;Weizheng Fu ,&nbsp;Yuzhen Zhang ,&nbsp;Shuang Hu ,&nbsp;Xuan Chen","doi":"10.1016/j.jpba.2025.116704","DOIUrl":"10.1016/j.jpba.2025.116704","url":null,"abstract":"<div><div>In this paper, a supramolecular heat-shrinkable tubing liquid phase microextraction was developed for the enrichment and determination of flavonoids. The nonanol adsorbed by the heat-shrinkable tubing could induce the surfactant in aqueous solution to self-assembly on its surface, and formed nonanol/CTAB supramolecules in a directional arrangement. The supramolecules structure enhanced the enrichment efficiency of five flavonoids. The parameters affecting the extraction efficiency were optimized, such as the type of organic solvent, concentration of surfactant, pH and volume of sample phase, salt concentration of sample solution, stirring speed and extraction time. The methodological investigation was carried out under the optimal experimental conditions. The enrichment factors of the five analytes were between 72.3 and 93.3. The concentrations of the target analytes showed good linearity between 0.2 and 5 µg/mL, 0.01–1 µg/mL, 0.01–5 µg/mL, respectively. The limit of detection was no more than 25 ng/mL. The procedure was successfully applied in extraction of five flavonoids from the natural products according to its average recoveries of 98.3–101.7 %.</div></div>","PeriodicalId":16685,"journal":{"name":"Journal of pharmaceutical and biomedical analysis","volume":"258 ","pages":"Article 116704"},"PeriodicalIF":3.1,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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