Journal of Parasitology最新文献

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A NEW PSEUDAPATEMON (DIGENEA: DIPLOSTOMOIDEA) FROM THE AMERICAN WOODCOCK AND REDESCRIPTION OF PSEUDAPATEMON ALDOUSI. 美洲伍德考克一种新假蝇目(diagenia: diplostomo总科)及aldousi假蝇目再描述。
IF 1.1 4区 医学
Journal of Parasitology Pub Date : 2026-03-31 DOI: 10.1645/25-103
Roxanne M Gasperetti, Sarah A Orlofske, Vasyl V Tkach, Tyler J Achatz
{"title":"A NEW PSEUDAPATEMON (DIGENEA: DIPLOSTOMOIDEA) FROM THE AMERICAN WOODCOCK AND REDESCRIPTION OF PSEUDAPATEMON ALDOUSI.","authors":"Roxanne M Gasperetti, Sarah A Orlofske, Vasyl V Tkach, Tyler J Achatz","doi":"10.1645/25-103","DOIUrl":"10.1645/25-103","url":null,"abstract":"<p><p>Pseudapatemonis a genus of diplostomoidean digeneans in which adults parasitize the intestines of scolopacid shorebirds. In this study, we describe a new species of Pseudapatemon collected from American woodcock (Scolopax minor) in Wisconsin and Nebraska. The new Pseudapatemon species is characterized by the distribution of vitellarium, which extends into the prosoma and holdfast organ. This is the fifth species of this genus and the third described from North America. We also provide a supplemental description of Pseudapatemon aldousi based on specimens from S. minor in Wisconsin. Our description provides an improved understanding of the holdfast organ and other major structures of P. aldousi, as our specimens were not as strongly curled as those described in previous studies.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"112 2","pages":"183-188"},"PeriodicalIF":1.1,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DIVERSITY, HOST RANGE, AND SPECIFICITY OF ECHINOSTOMES (FAMILY ECHINOSTOMATIDAE; LOOSS, 1899) IN THE MIDWESTERN UNITED STATES. 美国中西部棘孔虫(棘孔虫科;looss, 1899)的多样性、寄主范围和特异性。
IF 1.1 4区 医学
Journal of Parasitology Pub Date : 2026-03-26 DOI: 10.1645/24-138
Grace A Schumacher, Dennis J Minchella
{"title":"DIVERSITY, HOST RANGE, AND SPECIFICITY OF ECHINOSTOMES (FAMILY ECHINOSTOMATIDAE; LOOSS, 1899) IN THE MIDWESTERN UNITED STATES.","authors":"Grace A Schumacher, Dennis J Minchella","doi":"10.1645/24-138","DOIUrl":"10.1645/24-138","url":null,"abstract":"<p><p>Trematodes in the family Echinostomatidae (Looss, 1899) are globally distributed parasites of both humans and wildlife. In North America, echinostomes are nearly ubiquitous in freshwater systems, where they commonly parasitize snails as their first intermediate hosts, aquatic hosts such as snails or tadpoles as second intermediate hosts, and waterfowl and semi-aquatic mammals as their definitive hosts. Our study expands on sampling efforts in the midwestern United States and contributes novel ND1, ITS2, and 28S sequences from 12 lineages of echinostomes in the genera Echinostoma, Echinoparyphium, and Hypoderaeum. Additionally, COI barcoding and phylogenetic analysis of snail first intermediate hosts elucidated novel host-parasite combinations. Finally, we demonstrate that echinostomes are often not specific to 1 species of snail first intermediate host but rather to a family of aquatic snails, and we suggest that first intermediate hosts may drive echinostome speciation.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"112 2","pages":"170-182"},"PeriodicalIF":1.1,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
FLEAS AND FLEA-BORNE DISEASES IN SINGAPORE: DIVERSITY, TRENDS, AND FUTURE DIRECTIONS. 新加坡跳蚤和跳蚤传播的疾病:多样性、趋势和未来方向。
IF 1.1 4区 医学
Journal of Parasitology Pub Date : 2026-03-25 DOI: 10.1645/25-54
Mackenzie L Kwak, Michael W Hastriter, Ryo Nakao
{"title":"FLEAS AND FLEA-BORNE DISEASES IN SINGAPORE: DIVERSITY, TRENDS, AND FUTURE DIRECTIONS.","authors":"Mackenzie L Kwak, Michael W Hastriter, Ryo Nakao","doi":"10.1645/25-54","DOIUrl":"10.1645/25-54","url":null,"abstract":"<p><p>Understanding and controlling vectors and vector-borne diseases is critical for maintaining public health in Singapore. Although mosquitoes, ticks, and the diseases they cause have attracted strong attention from researchers in recent years, fleas and flea-borne diseases remain largely neglected in Singapore. To stimulate research on this neglected topic, we examine trends in flea-borne rickettsiosis in Singapore from 2016-2022. Trends in these data indicate that the majority of cases are locally acquired and are more common in males, migrant workers, and teens/young adults from ages 15-34. In addition, the bat flea Thaumapsylla breviceps is recorded from Singapore for the first time along with the first records of Ctenocephalides felis from Paradoxurus hermaphroditus and Canis familiaris in Singapore. A checklist of the fleas of Singapore with a host index is presented and the present state of knowledge on the diversity of fleas and flea-borne diseases in Singapore is discussed. Finally, a roadmap is presented to provide a direction for future research and public policy to more effectively address the challenges associated with fleas and flea-borne diseases in Singapore.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"112 2","pages":"162-169"},"PeriodicalIF":1.1,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ON THE IDENTITY OF ESTHIOPTERUM TATAUPA CARRIKER, 1936 (PSOCODEA: PHTHIRAPTERA: ISCHNOCERA): A FURNARIID LOUSE SPECIES. 论大灰鼠携带者的身份,1936(蜱总目:蝇翅目:蝇总目:蝇总目):一种家蝇。
IF 1.1 4区 医学
Journal of Parasitology Pub Date : 2026-03-25 DOI: 10.1645/25-75
Kamila M D Kuabara, Michel P Valim, Jason D Weckstein
{"title":"ON THE IDENTITY OF ESTHIOPTERUM TATAUPA CARRIKER, 1936 (PSOCODEA: PHTHIRAPTERA: ISCHNOCERA): A FURNARIID LOUSE SPECIES.","authors":"Kamila M D Kuabara, Michel P Valim, Jason D Weckstein","doi":"10.1645/25-75","DOIUrl":"10.1645/25-75","url":null,"abstract":"<p><p>Reexamination of the holotype of the louse species Esthiopterum tataupa Carriker, 1936 demonstrated that the species belongs to the genus Furnariphilus Price and Clayton, 1995 and is conspecific with Furnariphilus parkeri Price and Clayton, 1995. The Tataupa tinamou Crypturellus t. tataupa (Temminck, 1815), originally listed as the type host of this species, is not the true host. This association likely resulted from contamination during Carriker's 1934 Bolivian collections, when a furnariid (Sclerurus albigularis albicollis Carriker, 1935) was obtained at the same locality. The present study refines both the taxonomy and host association of this louse species and underscores the importance of continued critical evaluation of historical type specimens and refined morphological analysis. It also underscores the value of well-documented collections of hosts and parasites with associated field notes for assessing taxonomy and documenting ecological and evolutionary relationships of hosts and their parasites.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"112 2","pages":"158-161"},"PeriodicalIF":1.1,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147512978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CONVERGENT EVOLUTION BETWEEN ENDOSYMBIOTIC BACTERIA IN FEATHER-FEEDING LICE. 食羽虱内共生细菌的趋同进化。
IF 1.1 4区 医学
Journal of Parasitology Pub Date : 2026-03-23 DOI: 10.1645/25-63
Mariam Topchyan, Mohammad Mikail Bala, Kevin P Johnson, Kimberly K O Walden, Bret M Boyd
{"title":"CONVERGENT EVOLUTION BETWEEN ENDOSYMBIOTIC BACTERIA IN FEATHER-FEEDING LICE.","authors":"Mariam Topchyan, Mohammad Mikail Bala, Kevin P Johnson, Kimberly K O Walden, Bret M Boyd","doi":"10.1645/25-63","DOIUrl":"10.1645/25-63","url":null,"abstract":"<p><p>Parasitic feather-feeding lice have diets rich in amino acids but deficient in essential B vitamins. Around 22% of feather-feeding louse species are host to vertically inherited endosymbiotic bacteria belonging to the genus Sodalis. These endosymbiotic Sodalis species have undergone genome degeneration, with genes supporting amino acid synthesis being lost, while genes required for B vitamin synthesis are largely retained. However, several species of lice are host to endosymbionts belonging to proteobacteria genera other than Sodalis. It is unknown if these other endosymbionts have undergone a similar degenerative process. Here, we examine the genome of an endosymbiotic Enterobacter species, identified in a feather-feeding wing louse, asking if this endosymbiont arrived at a similar evolutionary outcome as Sodalis species. We find that this endosymbiotic Enterobacter species possesses a reduced genome and, similar to endosymbiotic Sodalis species, retains the genes required for the synthesis of B vitamins, complementing deficiencies in the louse's diet. This endosymbiont also lacks the ability to synthesize amino acids, which are abundant in the louse's diet. The data suggest that both Sodalis and Enterobacter species have independently converged on a common evolutionary solution in response to symbioses with feather-feeding lice. Thus, bacterial endosymbionts in these two different genera appear to be ecological replicates, providing lice with the same metabolic services in support of parasitism.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"112 2","pages":"150-157"},"PeriodicalIF":1.1,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147498835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
HOST-SHARING DYNAMICS AT PLAY: FIRST RECORD OF SPIROXYS CONTORTUS IN KINOSTERNON SCORPIOIDES FROM BRAZILIAN AMAZON. 宿主共享动态的作用:巴西亚马逊河流域kinosternon scorpioides中扭曲螺虫的首次记录。
IF 1.1 4区 医学
Journal of Parasitology Pub Date : 2026-03-20 DOI: 10.1645/25-59
Leandro Mauricio Oliveira Silva, Bianca Nandyara, Marlon Leal, Jeannie Nascimento Santos, Francisco Tiago Vasconcelos Melo
{"title":"HOST-SHARING DYNAMICS AT PLAY: FIRST RECORD OF SPIROXYS CONTORTUS IN KINOSTERNON SCORPIOIDES FROM BRAZILIAN AMAZON.","authors":"Leandro Mauricio Oliveira Silva, Bianca Nandyara, Marlon Leal, Jeannie Nascimento Santos, Francisco Tiago Vasconcelos Melo","doi":"10.1645/25-59","DOIUrl":"10.1645/25-59","url":null,"abstract":"<p><p>The genus Spiroxys Schneider, 1866 comprises species that commonly parasitize chelonians of the suborder Cryptodira found in the Holarctic and Asian regions. Spiroxys contortus (Rudolphi, 1819) was described as parasitizing a freshwater turtle of the suborder Cryptodira in the Palearctic region and is 1 of the most widely recorded species of the genus worldwide, with records even extending to Brazil. Thus, this study aims to report the first record of S. contortus in Kinosternon scorpioides (Linnaeus, 1766), a cryptodiran freshwater turtle of the family Kinosternidae, from 3 municipalities in the Brazilian Amazon. Nematodes were collected from the turtles' stomachs and identified with light microscopy. We identified several intraspecific variations and provide additional morphological and morphometric data for the species. This is the first record of S. contortus in K. scorpioides and the first record of the species in the Brazilian Amazon. Our findings expand the known host distribution of S. contortus, highlight host-sharing patterns among parasites of freshwater turtles, and contribute to understanding parasite diversity in K. scorpioides.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"112 2","pages":"140-149"},"PeriodicalIF":1.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147486494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CORONOEPIMERITUS BEASLEYARUM N. SP. (EUGREGARINIDA: ACTINOCEPHALOIDEA) FROM THE DIFFERENTIAL GRASSHOPPER, MELANOPLUS DIFFERENTIALIS (THOMAS), IN KANSAS. 来自美国堪萨斯州的微分蚱蜢,melanoplus differentialis (thomas)。
IF 1.1 4区 医学
Journal of Parasitology Pub Date : 2026-03-16 DOI: 10.1645/25-18
S K Vaughan, J J Cielocha
{"title":"CORONOEPIMERITUS BEASLEYARUM N. SP. (EUGREGARINIDA: ACTINOCEPHALOIDEA) FROM THE DIFFERENTIAL GRASSHOPPER, MELANOPLUS DIFFERENTIALIS (THOMAS), IN KANSAS.","authors":"S K Vaughan, J J Cielocha","doi":"10.1645/25-18","DOIUrl":"10.1645/25-18","url":null,"abstract":"<p><p>A description and differential diagnosis of Coronoepimeritus beasleyarum n. sp. are presented. Dissections of the differential grasshopper, Melanoplus differentialis (Thomas), collected in Johnson County, Kansas, were found to be parasitized by a previously undescribed species of parasite. Gregarines were located at the cecal junction between the foregut and midgut and in the anterior portion of the midgut. This new species is consistent with the generic diagnosis of Coronoepimeritus (Hoshide, 1959) Clopton & Clopton, 2022 in possessing oocysts with long, polar filament-like spines and a crown-like epimerite bearing a globular tumidus with unbranched digitiform processes. This species differs from other described Coronoepimeritus species in mixed-aged trophozoite and gamont total lengths (TL), oocyst length (OL), and deutomerite maximum width (DWM). It is the third species of Coronoepimeritus described from North American grasshoppers.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"112 2","pages":"133-139"},"PeriodicalIF":1.1,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147463484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
THE SALMONID ONCORHYNCHUS MYKISS AND BRYOZOAN FREDERICELLA BOREALIS SERVE AS ACTIVE HOSTS FOR THE NORTH AMERICAN STRAIN OF TETRACAPSULOIDES BRYOSALMONAE, THE CAUSE OF PROLIFERATIVE KIDNEY DISEASE. 鲑科oncorhynchus mykiss和苔藓虫fredericella borealis是北美四类苔藓菌的活跃宿主,这是导致增生性肾脏疾病的原因。
IF 1.1 4区 医学
Journal of Parasitology Pub Date : 2026-03-09 DOI: 10.1645/25-47
N Das, S D Atkinson, S L Hallett
{"title":"THE SALMONID ONCORHYNCHUS MYKISS AND BRYOZOAN FREDERICELLA BOREALIS SERVE AS ACTIVE HOSTS FOR THE NORTH AMERICAN STRAIN OF TETRACAPSULOIDES BRYOSALMONAE, THE CAUSE OF PROLIFERATIVE KIDNEY DISEASE.","authors":"N Das, S D Atkinson, S L Hallett","doi":"10.1645/25-47","DOIUrl":"10.1645/25-47","url":null,"abstract":"<p><p>The myxozoan parasite responsible for proliferative kidney disease (PKD), Tetracapsuloides bryosalmonae, alternates between salmonid fishes and freshwater bryozoans to complete its life cycle. However, no active hosts (species that shed viable, infectious spores) for the North American (NA) strain have been identified. We used fish-to-bryozoan and bryozoan-to-fish transmission experiments to assess the active host status of steelhead trout [Oncorhynchus mykiss] and the bryozoan Fredericella borealis. Specific pathogen-free F. borealis colonies were first exposed to effluent from naturally infected O. mykiss and monitored weekly using stereomicroscopy for overt T. bryosalmonae infections. Malacospores from infected colonies were then introduced to naïve O. mykiss, which were held at 16 C and sampled for polymerase chain reaction (PCR) and histology 2 and 8 wk post-exposure. Additionally, statoblasts and hatched zooids from overtly infected F. borealis colonies were screened with stereomicroscopy and quantitative real-time PCR (qPCR) to investigate transmission of T. bryosalmonae from zooids to statoblasts. Our results demonstrate that subclinically-infected O. mykiss shed viable fish malacospores of the NA strain of T. bryosalmonae, as evidenced by the development of overt infections in exposed F. borealis. Malacospores shed from the bryozoan subsequently induced clinical PKD in naïve O. mykiss, which exhibited kidney swelling upon necropsy, extrasporogonic stages of the parasite in histology, and PCR detections of T. bryosalmonae DNA. We found no evidence of vertical transmission within F. borealis. Given the widespread rearing and stocking of O. mykiss across North America, these findings highlight the importance of pre-release surveillance in susceptible stocks to prevent dissemination of T. bryosalmonae in habitats where suitable host populations may overlap.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"112 2","pages":"122-132"},"PeriodicalIF":1.1,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147390416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
HAEMOSPORIDIANS AS POTENTIAL SOURCES OF GEOGRAPHIC MARKERS FOR IRRUPTIVE BLACK-CAPPED CHICKADEES. 血孢子虫作为入侵黑冠山雀地理标记的潜在来源。
IF 1.1 4区 医学
Journal of Parasitology Pub Date : 2026-02-26 DOI: 10.1645/25-5
Ari A Rice, Robert L Curry, Jason D Weckstein
{"title":"HAEMOSPORIDIANS AS POTENTIAL SOURCES OF GEOGRAPHIC MARKERS FOR IRRUPTIVE BLACK-CAPPED CHICKADEES.","authors":"Ari A Rice, Robert L Curry, Jason D Weckstein","doi":"10.1645/25-5","DOIUrl":"10.1645/25-5","url":null,"abstract":"<p><p>Migratory birds can carry parasites thousands of miles, and therefore their parasite communities may provide an informative means of ascertaining the breeding and wintering locations of their hosts. However, avian parasites (particularly haemosporidians) are rarely used for this purpose because their usefulness is conditional upon knowing where and when birds acquired them during their annual cycles. In this study, we evaluated haemosporidian assemblages among non-resident black-capped chickadees (Poecile atricapillus), which occasionally \"irrupt\" south from parts of their northern distributional range to spend winters in the mid-Atlantic and Midwest regions of the United States. Specifically, we compared haemosporidian prevalence and lineage diversity from irruptive black-capped chickadees to those of resident Carolina chickadees (Poecile carolinensis) and black-capped × Carolina hybrids at 4 southeastern Pennsylvania locations, where most irruptive black-capped chickadees could be genetically and phenotypically identified among local birds. We hoped to (1) gauge whether certain haemosporidian lineages could help identify irruptive individuals among populations of otherwise sedentary black-capped chickadees and (2) roughly estimate where these irruptive birds hatched or bred. Irruptive birds had higher rates of haemosporidian infection than residents, and irruptive birds exclusively harbored a single Plasmodium lineage (BT7). Literature review suggests that lineage BT7 is widespread across the Northern hemisphere but is unlikely to be transmitted between chickadees in our study location. Thus, the presence of lineages such as BT7 may be useful for assessing the composition of seasonally sympatric bird populations with different migratory strategies or at least for identifying non-resident individuals within these populations.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"112 1","pages":"112-121"},"PeriodicalIF":1.1,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147317287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
HOOKSNIP: A BROWSER TOOL FOR DESIGNING ALLELE-SPECIFIC PCR PRIMERS IN HOOKWORM. hookclip:用于设计钩虫等位基因特异性PCR引物的浏览器工具。
IF 1.1 4区 医学
Journal of Parasitology Pub Date : 2026-02-26 DOI: 10.1645/25-88
Catherine A Jackson, John M Hawdon, Damien M O'Halloran
{"title":"HOOKSNIP: A BROWSER TOOL FOR DESIGNING ALLELE-SPECIFIC PCR PRIMERS IN HOOKWORM.","authors":"Catherine A Jackson, John M Hawdon, Damien M O'Halloran","doi":"10.1645/25-88","DOIUrl":"10.1645/25-88","url":null,"abstract":"<p><p>Surveillance for anthelmintic resistance in hookworms requires low-cost, field-deployable genotyping strategies. Allele-specific PCR (AS-PCR) is attractive because it discriminates single-nucleotide polymorphisms (SNPs) at the 3' end of a primer without sequencing infrastructure, thereby enabling rapid detection of drug-resistance markers directly from eggs or larvae. Here, we present hookSNiP (https://ohalloranlab.net/hooksnip), a browser-based application that designs allele-specific primer pairs for Ancylostoma species. The tool provides curated reference genomes, validation of variant call format (VCFs) and genome sequence compatibility, strand-aware primer orientation using gene annotations, and tiered rescue modes when strict primer design constraints cannot be met. By combining these features with AS-PCR, hookSNiP reduces practical barriers to SNP validation and expands the feasibility of large-scale resistance monitoring.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"112 1","pages":"109-111"},"PeriodicalIF":1.1,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12999167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147317252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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