Journal of Parasitology最新文献

{"title":"A RANGE-WIDE ECTOPARASITE SURVEY FOR ALLEGHENY WOODRATS (NEOTOMA MAGISTER)","authors":"Karen E. Powers, Ralph P. Eckerlin, Robert R. Sheehy, Richard J. Reynolds","doi":"10.1645/23-118","DOIUrl":"https://doi.org/10.1645/23-118","url":null,"abstract":"<p>Allegheny woodrats (<em>Neotoma magister</em>) are karst-specializing rodents that are rare or in conservation need in many states within their current range. Parasitism and habitat fragmentation have been suggested as primary reasons for declining populations. The presence, prevalence, and impact of ectoparasites, including fleas, ticks, and bots, is not fully understood rangewide. We collected Allegheny woodrat ectoparasites across 8 states in their range, identifying parasites via morphological and genetic means. Across contributions from 8 states, we discovered 2 woodrat-specific fleas parasitizing Allegheny woodrats: <em>Orchopeas pennsylvanicus</em> (all contributing states, n = 228) and <em>Epitedia cavernicola</em> (Indiana only, n = 9). The former was a new state record in New Jersey and Ohio. Woodrat specialists <em>Ixodes woodi</em> were morphologically identified as the dominant tick species (n = 38), and our contributions to genetic databases may ease confusion in future efforts. Three generalist species of ticks representing 8 individuals were identified as <em>Dermacentor variabilis</em>, <em>Amblyomma americanum</em>, and <em>Ixodes scapularis</em>. Only 2 bot fly species were recognized in Allegheny woodrats: 1 squirrel bot (<em>Cuterebra emasculator</em>) and 10 individuals of <em>Cuterebra</em> sp. not genetically conspecific to any known eastern U.S. rodent bot. The host specificity for fleas is not surprising, given that previous small-scale surveys and ticks primarily appear to be a mix of genus-specific (<em>Ixodes woodi</em>) and generalist species. There remains uncertainty with bots via morphological and genetic analyses. Our survey presents a wide-ranging baseline survey for Allegheny woodrats across their range, emphasizing the diversity (or specificity) of parasite groups for this species. An understanding of Allegheny woodrats and the health impact of ectoparasites is imperative because they face myriad challenges rangewide, especially considering the bot-driven demise of 1 woodrat in our study. Ectoparasites can have a marked impact on already-declining woodrat populations across their range and should not be overlooked in future surveys.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"28 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140636845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DESCRIPTION OF FEMALE DENDRITOBILHARZIA PULVERULENTA () FROM TWO NEW AVIAN HOSTS IN NAMIBIA WITH PHYLOGENETIC ANALYSES AND COMMENTS ON SEVERAL TAXONOMICALLY UNCERTAIN AVIAN SCHISTOSOME SEQUENCES","authors":"Haley R. Dutton, Louis H. DuPreez, Edward C. Netherlands, Bernard J. Jordaan, Stephen A. Bullard","doi":"10.1645/23-44","DOIUrl":"https://doi.org/10.1645/23-44","url":null,"abstract":"<p>During a 2021 parasitological survey of birds in the Nyae Nyae-Khaudum Dispersal Area (Kavango-Zambezi Transfrontier Conservation Area, Namibia), we collected 9 specimens of <em>Dendritobilharzia pulverulenta</em> (Braun, 1901) Skrjabin, 1924 infecting the blood (heart lumen) of a white-backed duck, <em>Thalassornis leuconotus</em> (Eyton, 1838) (Anseriformes: Anatidae), and a fulvous whistling duck, <em>Dendrocygna bicolor</em> (Vieillot, 1816) (Anatidae). These flukes were fixed for morphology and preserved for DNA extraction. We assigned our specimens to <em>Dendritobilharzia</em>Skrjabin and Zakharow, 1920 because they were strongly dorso-ventrally flattened in both sexes and had an intestinal cyclocoel with a zig-zag common cecum with lateral dendritic ramifications, numerous testes posterior to the cyclocoel and flanking the dendritic ramifications, and a tightly compacted convoluted ovary as well as lacking an oral sucker, ventral sucker, and gynaecophoric canal. Further, our specimens were morphologically identical to previously published descriptions of <em>D. pulverulenta</em>. Sequences of the <em>28S</em> from our specimens were nearly identical to those identified as <em>D. pulverulenta</em> from North America (New Mexico), and our <em>28S</em> phylogenetic analysis recovered <em>D. pulverulenta</em> within a polytomy of other Gigantobilharziinae spp. The <em>CO1</em> phylogenetic analysis recovered a monophyletic <em>Dendritobilharzia</em> and, with low taxon sampling, a monophyletic <em>Gigantobilharzia</em>. This is the first record of a species of <em>Dendritobilharzia</em> infecting these ducks as well as the first record of an adult <em>Dendritobilharzia</em> from sub-Saharan Africa. The original description of adult <em>D. pulverulenta</em> (type locality: northern Sudan) was based on 2 males only, and hence the present study is the first description of female <em>D. pulverulenta</em> from Africa (the continent of the type locality). We reassign 2 Gigantobilharziinae spp. based on morphology and nucleotide evidence: <em>Gigantobilharzia ensenadense</em> (Lorenti, Brant, Gilardoni, Diaz, and Cremonte, 2022) Dutton and Bullard, n. comb., and <em>Gigantobilharzia patagonense</em> (Lorenti, Brant, Gilardoni, Diaz, and Cremonte, 2022) Dutton and Bullard, n. comb. We also comment on several avian schistosome sequences whose identities need confirmation or that likely have been misidentified.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"17 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140609596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF AMBLYOMMA SCUTATUM (ACARI: IXODIDAE) ACCIDENTALLY INTRODUCED IN ITALY","authors":"Annalisa Grisendi, Mattia Calzolari, Francesco Defilippo, Deborah Torri, Katia Marzani, Michele Dottori, Paolo Bonilauri, Giulia Maioli","doi":"10.1645/20-69","DOIUrl":"https://doi.org/10.1645/20-69","url":null,"abstract":"<p>Eight ticks were found in Comacchio (FE), Italy parasitizing a young black iguana (<em>Ctenosaura similis</em>) that had been accidentally transported in a commercial plant container from Costa Rica. Specimens were identified morphologically as <em>Amblyomma scutatum</em> and then confirmed by the barcoding of the mitochondrial cytochrome c oxidase subunit 1 gene. <em>Amblyomma scutatum</em> is a common tick known to infest reptiles in Central America, Mexico, and Venezuela, but not in Europe. In Italy, the possibility for this tick to become endemic is unlikely because of the absence of its principal hosts. Nevertheless, this finding confirms the high risk of introducing exotic species that is linked with global commerce and therefore the need for veterinary control of shipments.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"100 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140609831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HOST SWITCHING IN DICYEMIDS (PHYLUM DICYEMIDA)","authors":"Hiroaki Nakajima, Ayako Fukui, Kazutaka Suzuki, R. Yusrifar Kharisma Tirta, Hidetaka Furuya","doi":"10.1645/23-52","DOIUrl":"https://doi.org/10.1645/23-52","url":null,"abstract":"<p>Dicyemids (phylum Dicyemida) are the most common and most characteristic endosymbionts in the renal sacs of benthic cephalopod molluscs: octopuses and cuttlefishes. Typically, 2 or 3 dicyemid species are found in a single specimen of the host, and most dicyemids have high host specificity. Host-specific parasites are restricted to a limited range of host species by ecological barriers that impede dispersal and successful establishment; therefore, phylogenies of interacting groups are often congruent due to repeated co-speciation. Most frequently, however, host and parasite phylogenies are not congruent, which can be explained by processes such as host switching and other macro-evolutionary events. Here, the history of dicyemids and their host cephalopod associations were studied by comparing their phylogenies. Dicyemid species were collected from 8 decapodiform species and 12 octopodiform species in Japanese waters. Using whole mitochondrial cytochrome <em>c</em> oxidase subunit 1 (<em>COI</em>) sequences, a phylogeny of 37 dicyemid species, including 4 genera representing the family Dicyemidae, was reconstructed. Phylogenetic trees derived from analyses of <em>COI</em> genes consistently suggested that dicyemid species should be separated into 3 major clades and that the most common genera, <em>Dicyema</em> and <em>Dicyemennea,</em> are not monophyletic. Thus, morphological classification does not reflect the phylogenetic relationships of these 2 genera. Divergence (speciation) of dicyemid species seems to have occurred within a single host species. Possible host-switching events may have occurred between the Octopodiformes and Decapodiformes or within the Octopodiformes or the Decapodiformes. Therefore, the mechanism of dicyemid speciation may be a mixture of host switching and intra-host speciation. This is the first study in which the process of dicyemid diversification involving cephalopod hosts has been evaluated with a large number of dicyemid species and genera.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"6 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140609598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE COCCIDIA SPECIES PFEIFFERINELLA ELLIPSOIDES EXHIBITS SPATIAL AND SPECIES-SPECIFIC VARIATION IN PREVALENCE AMONG FRESHWATER SNAILS","authors":"Maria Reid Bollinger, Taylor Michelle Fiedor, Kyle Douglas Gustafson","doi":"10.1645/23-17","DOIUrl":"https://doi.org/10.1645/23-17","url":null,"abstract":"<p>Freshwater snails are commonly studied within the context of their role as intermediate hosts for digenetic trematodes. However, there are fundamental data deficiencies related to our understanding of directly transmitted parasites, such as coccidia, for freshwater snails. Because variation in coccidia pathogenicity and transmission among snail species likely has major impacts on snail community structure, we aimed to investigate the spatial distribution and prevalence of coccidia in several freshwater snail species throughout the Ozark and Ouachita Mountains ecoregions in Arkansas. We opportunistically collected 220 freshwater snails from 24 Ozark sites in summer 2022 and scanned fecal slides for the presence of coccidia. In summer 2023, we surveyed an additional 146 snails from 19 Ouachita sites. To test for apparent interactions among coccidia and trematodes, we scanned feces from a subset of snails (<em>Physa</em> and <em>Planorbella</em> in the Ozarks) that did not have concurrent trematode infections and from those that did. We observed oocysts that morphologically conformed to <em>Pfeifferinella ellipsoides</em> in 2 of the 9 snail taxa from 7 of the 43 sites. <em>Planorbella trivolvis</em> was infected at 2 of 6 sites in the Ozarks and 0 of 5 sites in the Ouachitas. <em>Physa</em> species were infected at 6 of 14 sites in the Ozarks and 0 of 12 sites in the Ouachitas. In the Ozarks, <em>Pl. trivolvis</em> had an overall prevalence of 0.13 (6 of 47), whereas individuals in the genus <em>Physa</em> had an overall prevalence of 0.08 (8 of 97). Our chi-square and Fisher exact tests revealed no significant evidence for trematode–coccidia competition or synergism within the two snail species. There were no other species infected, and we did not observe any coccidia in the snails from the Ouachitas. Our survey of 366 snails among 9 taxa and 43 sites represents the largest survey for freshwater snail coccidia to date and indicates that both <em>Pl. trivolvis</em> and <em>Physa</em> spp. may be primary hosts and/or reservoir hosts for <em>Pf. ellipsoides</em> in freshwater snail communities. The highly aggregated distribution of <em>Pf. ellipsoides</em> in northwestern Arkansas requires further investigation. Our results led to proposal of several hypotheses for additional research, including questions regarding the variation of coccidia host specificity and virulence.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"177 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140804342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INFLUENCE OF GROOMING ON PERMANENT ARTHROPOD ASSOCIATES OF BIRDS: CATTLE EGRETS, LICE, AND MITES.","authors":"Matthew M Waller, Hannah M Warr, Graham B Goodman, Sarah E Bush, Dale H Clayton","doi":"10.1645/23-85","DOIUrl":"10.1645/23-85","url":null,"abstract":"<p><p>Birds have a diverse community of \"permanent\" arthropods that complete their entire life cycle on the body of the host. Because some of these arthropods are parasites that reduce host fitness, birds control them by grooming, which consists of preening with the beak and scratching with the feet. Although preening is the primary component of grooming, scratching is essential for controlling arthropods on the head and neck, which cannot be preened. Several unrelated groups of birds have evolved comb-like pectinate claws on the middle toenail of each foot. We tested the role of these claws in the control of arthropods by experimentally removing teeth from the claws of captive western cattle egrets (Bubulcus ibis) infested with chewing lice (Insecta: Phthiraptera), feather mites (Acari: Sarcoptiformes), and nasal mites (Acari: Mesostigmata). After a period of 4 mo, we compared the abundance of arthropods on experimental birds to that of control birds with intact teeth. We used video to quantify the grooming rates of the captive birds, which groomed twice as much as wild birds. Experimental and control birds did not differ significantly in grooming time. Both groups virtually eradicated the chewing lice, but not feather mites or nasal mites. We found no support for the hypothesis that pectinate claws increase the efficiency of arthropod control by grooming. Experiments with wild birds are needed to test the hypothesis further under conditions in which birds devote less time to grooming.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"110 2","pages":"143-149"},"PeriodicalIF":1.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DULCITRANSVERSOTREMA N. GEN. (DIGENEA: TRANSVERSOTREMATIDAE) AND FIRST MORPHOLOGICAL AND NUCLEOTIDE-BASED CONFIRMATION OF THE LIFE CYCLE OF A TRANSVERSOTREMATID IN NORTH AMERICA.","authors":"Triet N Truong, Stephen A Bullard","doi":"10.1645/23-112","DOIUrl":"10.1645/23-112","url":null,"abstract":"<p><p>We propose Dulcitransversotrema n. gen. for the freshwater transversotrematids Dulcitransversotrema patialense (Soparkar, 1924) n. comb. and Dulcitransversotrema chauhani (Agrawal and Singh, 1960) n. comb. Dulcitransversotrema is unique by the combination of lacking an oral sucker and an extensively coiled uterus as well as having testes that are not deeply lobed and that abut or nearly abut the inner posterolateral margins of the digestive tract (vs. deeply lobed testes that abut the cyclocoel region of the digestive tract only or that do not abut the ceca), a pre-oral genital pore (vs. post-oral genital pore), an ovary that is anterior to and abuts or nearly abuts the sinistral testis (vs. an ovary that is separated from the sinistral testis by some distance or having an ovary that is median and immediately pre-testicular), non-embryonated eggs (vs. embryonated eggs), typically having none or sparse vitelline follicles between the testes and ceca (vs. having a wholly inter-cecal vitellarium comprising a pair of highly compacted clusters of vitelline follicles anterolateral to the testes or having numerous vitelline follicles between the testes and ceca), an oblong, median, and primarily inter-testicular vitelline reservoir (vs. an extensively elongate, pre-testicular vitelline reservoir that arches anteriorly around the sinistral testis), and a subterminal excretory pore opening on the dorsal body surface between the level of the cyclocoel and posterior body end (vs. a terminal excretory pore). We describe the redia and cercaria of Dulcitransversotrema cf. patialense from the red-rimmed melania snail, Melanoides tuberculata (Müller, 1774) (Cerithioidea: Thiaridae), and their corresponding adult from beneath the scales of the zebrafish, Danio rerio (Hamilton, 1822) (Cypriniformes: Danionidae). All hosts were sampled from a spring-fed earthen pond at a private aquaculture facility near Ruskin, Florida. No transversotrematid life cycle was known from North America previously. The large subunit ribosomal DNA and ribosomal internal transcribed spacer 2 sequences of D. cf. patialense from Florida were most similar to those from D. patialense infecting red-rimmed melania from Mayagüez, Puerto Rico and differed by 134 and 69 nucleotides, respectively. Both phylogenetic analyses recovered Dulcitransversotrema as monophyletic and sister to a clade comprising Transversotrema spp. plus Crusziella formosa Cribb, Bray, and Barker, 1992.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"110 2","pages":"127-140"},"PeriodicalIF":1.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140288393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ralph Peter Eckerlin, Ph.D. 10 February 1938-9 February 2024.","authors":"Lance A Durden","doi":"10.1645/24-25","DOIUrl":"https://doi.org/10.1645/24-25","url":null,"abstract":"","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"110 2","pages":"141-142"},"PeriodicalIF":1.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140856796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NEW RECORDS OF HELMINTHS OF THE JAGUAR IN MEXICO, WITH AN UPDATED LIST OF SPECIES IN THE AMERICAS","authors":"Jesús Alonso Panti-May, Oscar Retana-Guiascón, Wilson Isaias Moguel-Chin, David I. Hernández-Mena, Luis García-Prieto","doi":"10.1645/23-56","DOIUrl":"https://doi.org/10.1645/23-56","url":null,"abstract":"<p>An inventory of parasites infecting the jaguar (<em>Panthera onca</em>) across its distribution range is relevant for the conservation of this threatened big cat. In this study, we report the occurrence of helminths in a jaguar from Mexico using morphological techniques (cleared and stained mounts and scanning electron microscopy) and partial sequences of the 28S ribosomal RNA (<em>28S</em> rRNA) gene and the cytochrome c oxidase 1 mitochondrial (<em>COI</em>) gene. We also provide an updated list of helminth species reported in jaguars in the Americas. Three helminth taxa are identified in the jaguar examined from Mexico: <em>Toxocara cati</em>, <em>Physaloptera</em> sp., and <em>Taenia</em> sp. The new <em>28S</em> rRNA sequences of <em>To</em>. <em>cati</em>, <em>Physaloptera</em> sp., and <em>Taenia</em> sp. and the <em>COI</em> sequence of <em>Taenia</em> sp. corroborate the identity of the helminths isolated from this host. One hundred and twenty-nine records of helminths parasitizing jaguars from 49 studies up to May 2023 were identified in the Americas. In most of these studies (73.6%), helminths were identified using coproparasitological techniques. Sixteen helminths (7 nematodes, 5 cestodes, 3 acanthocephalans, and 1 trematode) were identified at the species level in free-ranging and captive jaguars. The study demonstrates the value of an integrative taxonomy approach to increase the accuracy of parasite identification in wildlife, especially when helminth specimens are scarce or poorly fixed.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"41 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140165700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"METARHIZIUM ANISOPLIAE SENSU LATO NATIVE TO LIVESTOCK SOILS CAUSES HIGH MORTALITY ON RHIPICEPHALUS MICROPLUS LARVAE, ADULTS AND AFFECTS THEIR REPRODUCTION","authors":"F. Velázquez-Sarmiento, R. I. Rodríguez-Vivas, M. A. Alonso-Díaz, A. Fernández-Salas, D. Romero-Salas","doi":"10.1645/23-27","DOIUrl":"https://doi.org/10.1645/23-27","url":null,"abstract":"<p>The acaricidal effect of 14 strains of <em>Metarhizium anisopliae</em> sensu lato isolated from soil of livestock farms in the Mexican tropics was evaluated against larvae and engorged females, and during the laying and hatching of eggs of <em>Rhipicephalus microplus</em> (Ixodida: Ixodidae). For each fungal strain, the larvae mortality percentage was evaluated through a larval immersion test, while the reproductive efficiency indices in engorged females were measured using adult immersion tests at a dose of 1 × 10⁸ conidia/ml. All strains of <em>M. anisopliae</em> (s.l.) proved to be highly effective against <em>R. microplus</em> larvae (66–100%) and engorged females (100%). The strains also showed a good effect in inhibiting egg laying (16.45–56.38%) and a moderate effect in decreasing egg hatching (5.24–32.68%). Two strains demonstrated to be effective against all development phases of <em>R. microplus</em> in an integrated manner.</p>","PeriodicalId":16659,"journal":{"name":"Journal of Parasitology","volume":"11 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140165733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}