Journal of Orthopaedic Translation最新文献

{"title":"An innovative self-stabilised 3D-printed artificial vertebral body designed for clinical application and comparison with the conventional implants","authors":"Teng Zhang , Jian Li , Xinyu Li , Xin Pan , Xianlei Gao , Xiaojie Yang , Xiaolin Ma , Hao Li , Shiqing Feng , Zhongjun Liu","doi":"10.1016/j.jot.2025.04.010","DOIUrl":"10.1016/j.jot.2025.04.010","url":null,"abstract":"<div><h3>Background</h3><div>Corpectomy and bone defects reconstruction is a key surgical technique in spinal diseases treatment. Popular bone defect reconstruction methods include titanium mesh cage (TMC) fixation plate systems and traditional 3D-printed artificial vertebral body (3D-AVB). In our previous study, we conceptualised and created a self-stabilised 3D-printed artificial vertebral body (3D-SAVB) system and tested its clinical safety and efficacy, but have not compared with the conventional implants. This study was designed to compare our innovative 3D-SAVB system with a conventional 3D-AVB system, both mechanically and clinically.</div></div><div><h3>Methods</h3><div>This study included 33 patients with cervical spondylotic myelopathy who underwent single-level ACCF using the TMC, 3D-AVB, and 3D-SAVB systems. The operation time, intraoperative blood loss, neurological function recovery rate, average subsidence length, and cervical lordosis correction (C2-7 Cobb angle change) rates of the TMC, 3D-AVB, and 3D-SAVB groups were tested to compare their performance, and we selected four representative clinical cases with various diseases who underwent 3D-SAVB surgery for follow-up studies to demonstrate the clinical effect. In addition, finite element analysis was used to compare the stability, stress distribution, and artificial vertebral body stress of the 3D-SAVB, 3D-AVB, and TMC systems.</div></div><div><h3>Results</h3><div>The neurological function recovery rates of the TMC (84.8 ± 10.7 %), 3D-AVB (74.3 ± 7.9 %), and SAVB (85.99 ± 13.2 %) groups showed no significant difference (p > 0.05). The mean operation time of the TMC group (119.3 ± 21.5 min) is significant more than the 3D-SAVB (76.1 ± 23.1 mm) and 3D-AVB (82.6 ± 21.3 mm) groups (p < 0.05). The intraoperative blood loss of the TMC group (218.2 ± 51.5 ml) was significantly greater than that of the 3D-SAVB (187.6 ± 43.2 ml) and 3D-AVB groups (195.6 ± 31.3 ml) (p < 0.05). The mean subsidence length of the TMC group (3.5 ± 0.6 mm) was significantly greater than the 3D-AVB (1.3 ± 0.5 mm, p < 0.001) and 3D-SAVB (1.2 ± 1.1 mm, p = 0.002). The lordosis correction (C2-7 Cobb angle change) rate of the 3D-SAVB [(60.38 ± 6.2)%] and 3D-AVB [(61.4 ± 7.9)%] groups was significantly higher than that of the TMC group [(32.35 ± 3.7)%] (p < 0.05). Patients treated with the 3D-SAVB system achieved satisfactory treatment results with no postoperative complications during the follow-up period. The failed TMC fixation plate system underwent revision surgery using 3D-SAVB and demonstrated a superior prognosis. The biomechanical test showed that the 3D-SAVB system had greater longitudinal stability (p < 0.01), better stress distribution (p < 0.01), and less vertebral stress (p < 0.01) than the 3D-AVB and TMC systems.</div></div><div><h3>Conclusion</h3><div>These results demonstrate the mechanical advantages and great clinical application potential of our innovative 3D-SAVB system ","PeriodicalId":16636,"journal":{"name":"Journal of Orthopaedic Translation","volume":"53 ","pages":"Pages 52-62"},"PeriodicalIF":5.9,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144222925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Chk2 deletion rescues bone loss and cellular senescence induced by Bmi1 deficiency via regulation of Cyp1a1” [J Orthop Translat, 52 (2025) 360–375 /doi.org/10.1016/j.jot.2025.04.014]","authors":"Yining Liu , Xiaolei Ji , Jinge Zhang , Jinhong Lu , Boyang Liu , Haijian Sun , Dengshun Miao","doi":"10.1016/j.jot.2025.05.009","DOIUrl":"10.1016/j.jot.2025.05.009","url":null,"abstract":"","PeriodicalId":16636,"journal":{"name":"Journal of Orthopaedic Translation","volume":"53 ","pages":"Page 37"},"PeriodicalIF":5.9,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144211907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination therapy using intestinal organoids and their extracellular vesicles for inflammatory bowel disease complicated with osteoporosis","authors":"Mingkai Wang ,&nbsp;Ruiyang Li ,&nbsp;Shihao Sheng ,&nbsp;Zhenglin Dong ,&nbsp;Long Bai ,&nbsp;Xiuhui Wang ,&nbsp;Jianhua Wang ,&nbsp;Yuxiao Lai ,&nbsp;Xiao Chen ,&nbsp;Jie Gao ,&nbsp;Chongru He ,&nbsp;Han Liu ,&nbsp;Jiacan Su","doi":"10.1016/j.jot.2025.05.008","DOIUrl":"10.1016/j.jot.2025.05.008","url":null,"abstract":"<div><h3>Background</h3><div>Inflammatory bowel disease (IBD) with osteoporosis (OP) exhibits a clinically significant comorbidity, for which no effective treatment is currently available. Intestinal organoids (IOs), engineered through three-dimensional (3D) coculture systems, demonstrated intrinsic regenerative potentials. Additionally, extracellular vesicles derived from IOs (IOEVs) have been identified as potent nanoscale mediators capable of modulating intestinal inflammation.</div></div><div><h3>Methods</h3><div>In this study, we successfully established IOs and isolated IOEVs. miRNA sequencing in IOEVs revealed IBD-associated miRNAs, which may alleviate inflammatory response and have osteogenic effects. An in vitro model of IBD was established using lipopolysaccharide (LPS) to induce inflammation. Additionally, the dextran sulfate sodium (DSS)-induced IBD mouse model was employed to evaluate in vivo effects.</div></div><div><h3>Results</h3><div>In the LPS-induced in vitro model, treatment with IOs and IOEVs resulted in reduced cell necrosis and apoptosis. In DSS-induced IBD mouse models, treatment led to restoration of body weight and colon morphology. Histological assessment revealed an increase in intestinal crypts and normalization of tissue architecture. Immunological analyses showed upregulation of ZO-1 and Ki67 and downregulation of Caspase-3, suggesting enhanced mucosal barrier integrity and cellular proliferation with decreased apoptosis. Cytokine profiling showed downregulation of pro-inflammatory cytokines TNF-α, IL-1β, IL-6 and upregulation of anti-inflammatory cytokine IL-10. Importantly, the combination of IOs and IOEVs reversed osteoporosis progression in IBD, improving bone mass and quality.</div></div><div><h3>Conclusion</h3><div>Collectively, these multimodal findings establish a novel paradigm for gut–bone axis modulation through organoid-derived biologics, offering a promising therapeutic strategy for managing IBD-associated osteoporosis.</div></div><div><h3>The translational potential of this article</h3><div>This study highlights the translational potential of intestinal organoids and their extracellular vesicles as a dual-action biologic therapy that alleviates intestinal inflammation and reverses bone loss in IBD-associated osteoporosis. The identification of functional miRNAs within IOEVs supports their development as minimally invasive, cell-free therapeutics for systemic complications in inflammatory disease.</div></div>","PeriodicalId":16636,"journal":{"name":"Journal of Orthopaedic Translation","volume":"53 ","pages":"Pages 26-36"},"PeriodicalIF":5.9,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144196329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activation of LAMP1-mediated lipophagy by sulforaphane inhibits cellular senescence and intervertebral disc degeneration","authors":"Tianyu Qin ,&nbsp;Ming Shi ,&nbsp;Yongheng Xie ,&nbsp;Naibo Feng ,&nbsp;Chungeng Liu ,&nbsp;Ke Chen ,&nbsp;Yining Chen ,&nbsp;Wanli Zheng ,&nbsp;Mingxi Zhu ,&nbsp;Songlin Peng ,&nbsp;Guozhi Xiao ,&nbsp;Houqing Long","doi":"10.1016/j.jot.2025.05.010","DOIUrl":"10.1016/j.jot.2025.05.010","url":null,"abstract":"<div><h3>Background</h3><div>Intervertebral disc degeneration (IDD) is a major cause of chronic low back pain, involving lipid dysregulation and cellular senescence in nucleus pulposus (NP) cells. However, the relationship between lipid accumulation and cellular senescence in IDD remain unclear. This study aims to investigate whether lipid accumulation promotes NP cell senescence and explore the role of LAMP1-mediated lipophagy in mitigating these effects.</div></div><div><h3>Methods</h3><div>Human and rat NP tissue samples were analyzed for lipid levels and senescence markers, including p16, p21 and p53. NP cells were treated with palmitic acid (PA) to induce lipid accumulation. Multi-omics analysis and machine learning were used to identify LAMP1 as a key regulator of lipid metabolism in NP cells. The effects of LAMP1 overexpression on lipid clearance and cellular senescence were evaluated in vitro. The natural compound sulforaphane (SFN) was applied to stimulate LAMP1-mediated lipophagy. LAMP1 knockdown was used to assess the role of LAMP1 in SFN-induced lipophagy and its impact on lipid accumulation and senescence. In vivo, SFN treatment was administered to rats with IDD induced by needle puncture. MRI, X-ray, and histological analysis were performed to evaluate the effects of SFN on disc degeneration, lipid accumulation, and senescence in NP tissue.</div></div><div><h3>Results</h3><div>Excessive lipid accumulation in degenerated NP tissues was observed, along with increased expression of senescence markers. Further experiments demonstrated that LAMP1 overexpression reduced lipid accumulation and senescence in NP cells. Notably, the natural compound sulforaphane enhanced LAMP1-mediated lipophagy, promoting lipid clearance and reducing senescence. In vivo, sulforaphane treatment in a rat IDD model reduced lipid accumulation and delayed IDD.</div></div><div><h3>Conclusion</h3><div>Our findings suggest that LAMP1-mediated lipophagy plays a crucial role in inhibiting NP cell senescence and that sulforaphane can slow the progression of IDD by activating LAMP1.</div></div><div><h3>The translational potential of this article</h3><div>This study indicates that the therapeutic effects of sulforaphane in mitigating lipid accumulation and senescence can provide an effective treatment strategy for delaying the progression of IDD in the future.</div></div>","PeriodicalId":16636,"journal":{"name":"Journal of Orthopaedic Translation","volume":"53 ","pages":"Pages 12-25"},"PeriodicalIF":5.9,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144196328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From cells to clinic: Single-cell transcriptomics shaping the future of orthopedics","authors":"Qiuyuan Wang ,&nbsp;Moli Huang ,&nbsp;Jiong Jiong Guo","doi":"10.1016/j.jot.2025.05.001","DOIUrl":"10.1016/j.jot.2025.05.001","url":null,"abstract":"<div><div>Single-cell RNA sequencing (scRNA-seq) technology hold significant potential for advancing orthopedic research. This review examines the impact of ScRNA-seq on the future development of orthopedic research and practice. In the study of osteoarthritis, scRNA-seq can finely characterize the changes in the subsets of chondrocytes and their role in disease progression. In rheumatoid arthritis, this technique reveals the complex heterogeneity and cell-to-cell interactions between fibroblasts and immune cells. ScRNA-seq offers insights into the heterogeneity of nucleus pulposus, annulus fibrosus, and endplate cells, providing a novel perspective on the pathological mechanisms of intervertebral disc degeneration. Single-cell analysis in osteosarcoma research has uncovered the complexity of the tumor microenvironment and mechanisms of immunosuppression. Through these studies, scRNA-seq enhances insights into disease pathogenesis and offers innoviate approaches for precision medicine and personalized treatment strategies.</div></div><div><h3>The Translational Potential of this Article</h3><div>This article systematically reviews the cellular heterogeneity, molecular mechanisms and immune microenvironment of orthopedic diseases (such as osteoarthritis, rheumatoid arthritis, intervertebral disc degeneration, osteosarcoma) by single-cell RNA sequencing (scRNA-seq), which provides a theoretical basis for accurate diagnosis, new therapeutic target discovery (such as TRPV1, CXCR4) and individualized treatment strategies. The combination of multi-omics and spatial transcriptome technology is expected to accelerate clinical translation and optimize the diagnosis and treatment system of orthopedic diseases.</div></div>","PeriodicalId":16636,"journal":{"name":"Journal of Orthopaedic Translation","volume":"53 ","pages":"Pages 1-11"},"PeriodicalIF":5.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“Multidisciplinary synergy driving innovation in orthopaedic translational medicine”","authors":"Qiuli QI,&nbsp;Wing Hoi Cheung (Prof),&nbsp;Peter Hegyi (Prof)","doi":"10.1016/j.jot.2025.06.001","DOIUrl":"10.1016/j.jot.2025.06.001","url":null,"abstract":"","PeriodicalId":16636,"journal":{"name":"Journal of Orthopaedic Translation","volume":"52 ","pages":"Pages A1-A3"},"PeriodicalIF":5.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144632098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to ‘Guideline for adolescent scoliosis screening in China (Public Version 2024)’ [J Orthop Translation, Volume 50, January 2025, Pages 364-372]","authors":"Chinese Orthopedic Association,&nbsp;Yu Zhao ,&nbsp;Yan Zhao ,&nbsp;Sheng Lu ,&nbsp;Qiang Yang ,&nbsp;Liang Chen ,&nbsp;Xinlong Ma ,&nbsp;Guixing Qiu","doi":"10.1016/j.jot.2025.03.008","DOIUrl":"10.1016/j.jot.2025.03.008","url":null,"abstract":"","PeriodicalId":16636,"journal":{"name":"Journal of Orthopaedic Translation","volume":"52 ","pages":"Page 492"},"PeriodicalIF":5.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144632104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical effects in aging of the musculoskeletal system: Molecular signaling and spatial scale alterations","authors":"Zeyuan Zhang ,&nbsp;Fuming Cao ,&nbsp;Dingfa Liang ,&nbsp;Meng Pan ,&nbsp;William W. Lu ,&nbsp;Houchen Lyu ,&nbsp;Yong Xie ,&nbsp;Licheng Zhang ,&nbsp;Peifu Tang","doi":"10.1016/j.jot.2025.04.018","DOIUrl":"10.1016/j.jot.2025.04.018","url":null,"abstract":"<div><div>The musculoskeletal system, the primary load-bearing structure of the human body, plays a crucial role in mechanotransduction, a process comprising mechanosensation, mechanotransduction, and mechanical effect. Aging leads to loss of ability of mechanosensitive cells to sense mechanical stimuli, disruption of transduction pathways, SASP and adiposity accumulation. At the mesoscopic level, bone, cartilage, and muscle differentiation decline, while adipogenesis increases, leading to extracellular matrix and structural aging, ultimately manifesting as macroscopic musculoskeletal degeneration. This review explores intercellular crosstalk and mechanotransduction alterations in aging from a mechanobiological perspective, providing insights into potential therapeutic targets for bone aging and osteoporosis. It also introduces the mesoscopic scale definition and trans mesoscopic transplantation therapy as novel strategies for fracture treatment, postoperative rehabilitation, and bone regeneration, offering innovative directions for future musculoskeletal research.</div></div><div><h3>The translational potential of this article</h3><div>This article systematically reviews the effects of aging on the musculoskeletal system from a mechanobiological viewpoint, covering from microscopic molecular signaling to macroscopic spatial structural alterations, and proposes new strategies to complement the principles of AO therapy, optimization of braking, new insights into tumor metastasis and weight-bearing, and a new strategy for trans mesoscopic transplantation therapy. These insights will contribute to optimizing the management of geriatric fragility fractures in the elderly, exploring innovative therapies for the treatment of diseases of the aging musculoskeletal system, and facilitating the development of integrative therapies and precision medicine in the field of orthopaedics.</div></div>","PeriodicalId":16636,"journal":{"name":"Journal of Orthopaedic Translation","volume":"52 ","pages":"Pages 464-477"},"PeriodicalIF":5.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144632102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and characterization of an inflammatory cartilaginous organoids model for organoid transplantation study","authors":"Yanchao Zhang ,&nbsp;Qing Fang ,&nbsp;Yue Peng ,&nbsp;Honglin Liu ,&nbsp;Jiancheng Tang ,&nbsp;Ruichen Ma ,&nbsp;Weiguo Wang","doi":"10.1016/j.jot.2025.05.002","DOIUrl":"10.1016/j.jot.2025.05.002","url":null,"abstract":"<div><h3>Background</h3><div>Transplantation of cartilaginous organoids for repairing cartilage defects in osteoarthritis represents a novel treatment approach. However, A controversial argument remains about whether cartilaginous organoids derived from the differentiation of bone marrow mesenchymal stem cells (BMSCs) in the three-dimensional (3D) environment are strictly organoids and whether the inflammatory microenvironment would affect the success rate of organoid transplantation. This study characterized 3D BMSC-derived cartilaginous organoids and developed an inflammatory organoid model to better understand the transcriptomic changes in the organoids induced by the microenvironment when transplanted into the knee with osteoarthritis.</div></div><div><h3>Methods</h3><div>Spatial growth BMSCs were generated and cultured in the cartilage differentiation medium to establish cartilaginous organoids. The model was characterized in both morphology and biology aspects. Subsequently, IL-1β induced inflammatory cartilaginous organoids were established and the transcriptomic sequencing was performed to investigate gene expression changes.</div></div><div><h3>Results</h3><div>BMSC-derived cartilaginous organoids were characterized by histology and immunofluorescence. Both Alcian blue and Safranin O staining revealed abundant articular cartilage extracellular matrix (ECM) in the organoids. The expression of cartilage specific ACAN and Col2A1 was confirmed by immunofluorescence. The organoids had the biological ability to repair cartilage defects. IL-1β induced inflammatory cartilaginous organoids were established and mRNA sequencing revealed downregulation of pathways related to cell adhesion and extracellular matrix organization. Upregulation of IL-6, TNF-α, CCL2 and CXCL1 was confirmed.</div></div><div><h3>Conclusion</h3><div>We thoroughly validated and characterized BMSC-derived cartilaginous organoids and established the inflammatory cartilaginous organoid models. This study revealed that the attenuation in cell adhesion and ECM formation of organoids induced by inflammatory chemokines may decrease the success rate and effectiveness of organoids auto-transplantation for fixing cartilage defects in the inflammatory microenvironment of the OA joint.</div></div><div><h3>The translational potential of this article</h3><div>By establishing and validating an in vitro inflammatory cartilaginous organoid model, this study provides a robust platform to examine how inflammatory mediators influence cartilage-like constructs. These findings enable the identification of targeted interventions to enhance the organoids’ resilience against the inflammatory environment commonly found in osteoarthritic joints. Ultimately, this strategy offers a novel avenue for improving transplant success and promoting cartilage defect repair in patients with OA, thereby contributing valuable insights and potential clinical applications in regenerative medicine.</div></div>","PeriodicalId":16636,"journal":{"name":"Journal of Orthopaedic Translation","volume":"52 ","pages":"Pages 376-386"},"PeriodicalIF":5.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial Transplantation/Transfer: Promising Therapeutic Strategies for Spinal Cord Injury","authors":"Xiaochun Xiong ,&nbsp;Chao Zhou ,&nbsp;Yijun Yu ,&nbsp;Qiong Xie ,&nbsp;Linying Xia ,&nbsp;Qingping Li ,&nbsp;Hongming Lin ,&nbsp;Songou Zhang ,&nbsp;Wenqing Liang","doi":"10.1016/j.jot.2025.04.017","DOIUrl":"10.1016/j.jot.2025.04.017","url":null,"abstract":"<div><div>Spinal cord injury (SCI) remains an unresolved and complex medical challenge. In SCI, mitochondrial dysfunction leads to calcium overload and an increase in reactive oxygen species (ROS). Intercellular mitochondrial transfer has the potential to rescue surviving neurons, while exogenous mitochondrial transplantation can be performed through direct injection or cell-assisted methods. This review explored the current state of research on mitochondrial transplantation and transfer as potential treatments for SCI. It also analyzed the therapeutic implications, influencing factors, and advanced delivery methods for both endogenous mitochondrial transfer and exogenous mitochondrial transplantation. Furthermore, future research directions, including optimizing mitochondrial delivery methods, determining optimal dosages for different delivery approaches, were discussed based on larger animal models and clinical trials. The goal of this review was to introduce novel concepts and prospects for SCI therapy and to contribute to the advancement of medical research in this field.</div></div><div><h3>The Translational Potential of This Article</h3><div>At present, SCI lacks effective therapies, with mitochondrial dysfunction playing a central role in neuronal damage. Mitochondrial transplantation holds promise for restoring bioenergetic function. However, key challenges remain, including optimizing delivery methods, determining appropriate dosages, scalability, donor mitochondrial sourcing, regulatory hurdles and ensuring successful integration. Addressing these issues requires non-invasive platforms, validation in large-animal models, and clinical trials. This approach may bridge mitochondrial biology with translational engineering, thereby advancing the development of regenerative therapies for SCI.</div></div>","PeriodicalId":16636,"journal":{"name":"Journal of Orthopaedic Translation","volume":"52 ","pages":"Pages 441-450"},"PeriodicalIF":5.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}