Journal of Nucleic Acids最新文献_第10页

Unwinding and rewinding: double faces of helicase? 解卷和复卷:解旋酶的双面?

IF 2.3

Journal of Nucleic Acids Pub Date : 2012-01-01 Epub Date: 2012-07-19 DOI: 10.1155/2012/140601

Yuliang Wu

引用次数: 82

Using chemical approaches to understand RNA structure and function in biology. 用化学方法了解RNA的结构和功能。

IF 2.3

Journal of Nucleic Acids Pub Date : 2012-01-01 Epub Date: 2012-02-16 DOI: 10.1155/2012/972575

Dmitry A Stetsenko, Arthur van Aerschot

{"title":"Using chemical approaches to understand RNA structure and function in biology.","authors":"Dmitry A Stetsenko, Arthur van Aerschot","doi":"10.1155/2012/972575","DOIUrl":"https://doi.org/10.1155/2012/972575","url":null,"abstract":"Interest of the research community in the aspects of chemical biology of RNA has increased vastly over the last twenty years, primarily due to the discovery of RNAi, our deepened understanding of the role of miRNA in the subtle regulation of vital cellular processes, and the realization of the fact that the RNA world—the realm where RNA plays the key parties as a self-replicating molecule and a universal catalyst—is still pretty much with us today as the ribosomal RNA performs its catalytic solo in the formation of the peptide bond in the ribosome. To answer the needs of Biology, Chemistry had, in turn, multiplied and perfected its approaches to study the molecular mechanisms underlying RNA functions in living systems. So, the idea behind this special issue is to show our readership a screenshot of what could be, and has been, achieved recently by applying chemical methods to solve the problems of RNA biology. Ten articles have been carefully selected out of the bunch of those submitted to provide, we believe, a balanced view of different facets of RNA structure and function and, also, of the array of chemical tools to enable us to peek into them.","PeriodicalId":16575,"journal":{"name":"Journal of Nucleic Acids","volume":"2012 ","pages":"972575"},"PeriodicalIF":2.3,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/972575","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30551706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Concept for Selection of Codon-Suppressor tRNAs Based on Read-Through Ribosome Display in an In Vitro Compartmentalized Cell-Free Translation System. 体外区隔化无细胞翻译系统中基于可读核糖体展示的密码子抑制trna选择概念

IF 2.3

Journal of Nucleic Acids Pub Date : 2012-01-01 Epub Date: 2012-07-18 DOI: 10.1155/2012/538129

Atsushi Ogawa, Masayoshi Hayami, Shinsuke Sando, Yasuhiro Aoyama

引用次数: 6

A two-piece derivative of a group I intron RNA as a platform for designing self-assembling RNA templates to promote Peptide ligation. 一组内含子RNA的两段衍生物，作为设计自组装RNA模板以促进肽连接的平台。

IF 2.3

Journal of Nucleic Acids Pub Date : 2012-01-01 Epub Date: 2012-08-22 DOI: 10.1155/2012/305867

Takahiro Tanaka, Hiroyuki Furuta, Yoshiya Ikawa

引用次数: 2

Recent advances in chemical modification of Peptide nucleic acids. 肽核酸化学修饰研究进展。

IF 2.3

Journal of Nucleic Acids Pub Date : 2012-01-01 Epub Date: 2012-09-06 DOI: 10.1155/2012/518162

Eriks Rozners

引用次数: 44

Alternative splicing in oncogenic kinases: from physiological functions to cancer. 致癌激酶的选择性剪接:从生理功能到癌症。

IF 2.3

Journal of Nucleic Acids Pub Date : 2012-01-01 Epub Date: 2011-10-05 DOI: 10.1155/2012/639062

Sabine Druillennec, Coralie Dorard, Alain Eychène

引用次数: 25

In vitro selection of fab fragments by mRNA display and gene-linking emulsion PCR. 用mRNA展示和基因连锁乳剂聚合酶链反应筛选fab片段。

IF 2.3

Journal of Nucleic Acids Pub Date : 2012-01-01 Epub Date: 2012-09-23 DOI: 10.1155/2012/371379

Takeshi Sumida, Hiroshi Yanagawa, Nobuhide Doi

{"title":"In vitro selection of fab fragments by mRNA display and gene-linking emulsion PCR.","authors":"Takeshi Sumida, Hiroshi Yanagawa, Nobuhide Doi","doi":"10.1155/2012/371379","DOIUrl":"https://doi.org/10.1155/2012/371379","url":null,"abstract":"In vitro selection by display methods has been an effective tool for engineering recombinant antibodies. mRNA display based on a cell-free translation system has the advantages of larger library sizes and quicker selection procedures compared with cell-based display methods such as phage display. However, mRNA display has been limited to select single-chain polypeptides such as scFvs due to its characteristic of linking a nascent polypeptide with its encoding mRNA on the ribosome. Here we demonstrated a new way of selecting heterodimeric Fab fragments by using mRNA display combined with emulsion PCR. We designed a pair of complementary 5' UTR sequences that can link the Fab heavy and light chain genes together by overlap-extension PCR in water-in-oil emulsions. We confirmed that two mRNA-displayed polypeptides for heavy and light chain of a model Fab fragment were associated into the active form and that a specific Fab fragment gene was enriched over 100-fold per round of a model affinity selection followed by the gene-linking emulsion PCR. We further performed directed evolution of Fab fragments with higher binding activity from a randomized Fab fragment library.","PeriodicalId":16575,"journal":{"name":"Journal of Nucleic Acids","volume":"2012 ","pages":"371379"},"PeriodicalIF":2.3,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/371379","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30965408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Genetic predisposition for dermal problems in hexavalent chromium exposed population. 六价铬暴露人群皮肤问题的遗传易感性。

IF 2.3

Journal of Nucleic Acids Pub Date : 2012-01-01 Epub Date: 2012-07-26 DOI: 10.1155/2012/968641

Priti Sharma, Vipin Bihari, Sudhir K Agarwal, Sudhir K Goel

{"title":"Genetic predisposition for dermal problems in hexavalent chromium exposed population.","authors":"Priti Sharma, Vipin Bihari, Sudhir K Agarwal, Sudhir K Goel","doi":"10.1155/2012/968641","DOIUrl":"https://doi.org/10.1155/2012/968641","url":null,"abstract":"We studied the effect of genetic susceptibility on hexavalent chromium induced dermal adversities. The health status of population was examined from the areas of Kanpur (India) having the elevated hexavalent chromium levels in groundwater. Blood samples were collected for DNA isolation to conduct polymorphic determination of genes, namely: NQO1 (C609T), hOGG1 (C1245G), GSTT1, and GSTM1 (deletion). Symptomatic exposed subjects (n = 38) were compared with asymptomatic exposed subjects (n = 108) along with asymptomatic controls (n = 148) from a non contaminated reference community. Exposed symptomatic group consisted of 36.8% subjects who were GSTM1 null genotyped as compared to asymptomatic where only 19.4% subjects were null. The exposed subjects with GSTM1 null genotype were more susceptible to dermal adversities in comparison with wild genotyped subjects (OR = 2.42; 95% CI = 1.071-5.451). Age, smoking, gender or duration of residence were not found to have any confounding effect towards this association. Association with other genes was not statistically significant, nonetheless, possible contribution by these genes cannot be ruled out. In conclusion, variation in the polymorphic status of GSTM1 gene may influence dermal outcomes among residents from Cr(VI) contaminated areas. Further studies are therefore, needed to examine these observations among different population groups.","PeriodicalId":16575,"journal":{"name":"Journal of Nucleic Acids","volume":"2012 ","pages":"968641"},"PeriodicalIF":2.3,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/968641","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30856615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Splicing programs and cancer. 剪接程序和癌症。

IF 2.3

Journal of Nucleic Acids Pub Date : 2012-01-01 Epub Date: 2011-10-24 DOI: 10.1155/2012/269570

Sophie Germann, Lise Gratadou, Martin Dutertre, Didier Auboeuf

引用次数: 46

Specific Roles of MicroRNAs in Their Interactions with Environmental Factors. microrna在与环境因子相互作用中的特殊作用。

IF 2.3

Journal of Nucleic Acids Pub Date : 2012-01-01 Epub Date: 2012-10-31 DOI: 10.1155/2012/978384

Juan Wang, Qinghua Cui

引用次数: 34