Journal of molecular endocrinology最新文献_第10页

20-Hydroxyecdysone activates the protective arm of the RAAS via the MAS receptor. 羟基蜕皮激素通过MAS受体激活RAAS的保护臂。

IF 3.5 4区医学

Journal of molecular endocrinology Pub Date : 2021-12-23 DOI: 10.1530/JME-21-0033

René Lafont, Maria Serova, Blaise Didry-Barca, Sophie Raynal, Louis Guibout, Laurence Dinan, Stanislas Veillet, Mathilde Latil, Waly Dioh, Pierre J Dilda

{"title":"20-Hydroxyecdysone activates the protective arm of the RAAS via the MAS receptor.","authors":"René Lafont, Maria Serova, Blaise Didry-Barca, Sophie Raynal, Louis Guibout, Laurence Dinan, Stanislas Veillet, Mathilde Latil, Waly Dioh, Pierre J Dilda","doi":"10.1530/JME-21-0033","DOIUrl":"https://doi.org/10.1530/JME-21-0033","url":null,"abstract":"20-Hydroxyecdysone (20E) is a steroid hormone that plays a key role in insect development through nuclear ecdysteroid receptors (EcR/RXR complex) and at least one membrane GPCR receptor (DopEcR). It also displays numerous pharmacological effects in mammals, where its mechanism of action is still debated, involving either an unidentified GPCR or the estrogen ERβ receptor. The goal of this study was to better understand 20E mechanism of action in mammals. A mouse myoblast cell line (C2C12) and the gene expression of myostatin (a negative regulator of muscle growth) were used as a reporter system of anabolic activity. Experiments using protein-bound 20E established the involvement of a membrane receptor. 20E-like effects were also observed with angiotensin(1-7), the endogenous ligand of MAS. Additionally, the effect on myostatin gene expression was abolished by Mas receptor knock-down using siRNA or pharmacological inhibitors. 17β-Estradiol (E2) also inhibited myostatin gene expression, but protein-bound E2 was inactive, and E2 activity was not abolished by angiotensin(1-7) antagonists. A mechanism involving cooperation between the MAS receptor and a membrane-bound palmitoylated estrogen receptor is proposed. The possibility to activate the MAS receptor with a safe steroid molecule is consistent with the pleiotropic pharmacological effects of ecdysteroids in mammals and, indeed, the proposed mechanism may explain the close similarity between the effects of angiotensin(1-7) and 20E. Our findings open up many possible therapeutic developments involving stimulation of the protective arm of the renin-angiotensin-aldosterone system (RAAS) with 20E.","PeriodicalId":16570,"journal":{"name":"Journal of molecular endocrinology","volume":"68 2","pages":"77-87"},"PeriodicalIF":3.5,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39660513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

GCgx: transcriptome-wide exploration of the response to glucocorticoids. GCgx:糖皮质激素应答的转录组范围探索。

IF 3.5 4区医学

Journal of molecular endocrinology Pub Date : 2021-12-10 DOI: 10.1530/JME-21-0107

Qilin Cao, Yamil Boo Irizarry, Svetlana Yazhuk, Thai Tran, Manasi Gadkari, Luis Miguel Franco

引用次数: 1

Non-cell autonomous mechanisms control mitochondrial gene dysregulation in polycystic ovary syndrome. 非细胞自主机制控制多囊卵巢综合征线粒体基因失调。

IF 3.5 4区医学

Journal of molecular endocrinology Pub Date : 2021-12-03 DOI: 10.1530/JME-21-0212

Alba Moreno-Asso, Ali Altıntaş, Luke C McIlvenna, Rhiannon K Patten, Javier Botella, Andrew J McAinch, Raymond J Rodgers, Romain Barrès, Nigel K Stepto

{"title":"Non-cell autonomous mechanisms control mitochondrial gene dysregulation in polycystic ovary syndrome.","authors":"Alba Moreno-Asso, Ali Altıntaş, Luke C McIlvenna, Rhiannon K Patten, Javier Botella, Andrew J McAinch, Raymond J Rodgers, Romain Barrès, Nigel K Stepto","doi":"10.1530/JME-21-0212","DOIUrl":"https://doi.org/10.1530/JME-21-0212","url":null,"abstract":"Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with insulin resistance and impaired energy metabolism in skeletal muscle, the aetiology of which is currently unclear. Here, we mapped the gene expression profile of skeletal muscle from women with PCOS and determined if cultured primary myotubes retain the gene expression signature of PCOS in vivo. Transcriptomic analysis of vastus lateralis biopsies collected from PCOS women showed lower expression of genes associated with mitochondrial function, while the expression of genes associated with the extracellular matrix was higher compared to controls. Altered skeletal muscle mRNA expression of mitochondrial-associated genes in PCOS was associated with lower protein expression of mitochondrial complex II-V, but not complex I, with no difference in mitochondrial DNA content. Transcriptomic analysis of primary myotube cultures established from biopsies did not display any differentially expressed genes between controls and PCOS. Comparison of gene expression profiles in skeletal muscle biopsies and primary myotube cultures showed lower expression of mitochondrial and energy metabolism-related genes in vitro, irrespective of the group. Together, our results show that the altered mitochondrial-associated gene expression in skeletal muscle in PCOS is not preserved in cultured myotubes, indicating that the in vivo extracellular milieu, rather than genetic or epigenetic factors, may drive this alteration. Dysregulation of mitochondrial-associated genes in skeletal muscle by extracellular factors may contribute to the impaired energy metabolism associated with PCOS.","PeriodicalId":16570,"journal":{"name":"Journal of molecular endocrinology","volume":"68 1","pages":"63-76"},"PeriodicalIF":3.5,"publicationDate":"2021-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8679849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39603154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

miR-23a/b-3p promotes hepatic lipid accumulation by regulating Srebp-1c and Fas. miR-23a/b-3p通过调节Srebp-1c和Fas促进肝脏脂质积累。

IF 3.5 4区医学

Journal of molecular endocrinology Pub Date : 2021-11-26 DOI: 10.1530/JME-20-0324

Linfang Li, Xiaoyi Zhang, Hangjiang Ren, Xiuqing Huang, Tao Shen, Weiqing Tang, Lin Dou, Jian Li

引用次数: 11

Downregulation of miR-216a-5p and miR-652-3p is associated with growth and invasion by targeting JAK2 and PRRX1 in GH-producing pituitary tumours. miR-216a-5p和miR-652-3p的下调通过靶向JAK2和PRRX1在gh产生的垂体肿瘤中与生长和侵袭相关。

IF 3.5 4区医学

Journal of molecular endocrinology Pub Date : 2021-11-26 DOI: 10.1530/JME-21-0070

Yang Jong Lee, Chan Woo Kang, Ju Hun Oh, Jean Kim, Jong-Pil Park, Ju Hyung Moon, Eui Hyun Kim, Soohyun Lee, Se Hoon Kim, Cheol Ryong Ku, Eun Jig Lee

{"title":"Downregulation of miR-216a-5p and miR-652-3p is associated with growth and invasion by targeting JAK2 and PRRX1 in GH-producing pituitary tumours.","authors":"Yang Jong Lee, Chan Woo Kang, Ju Hun Oh, Jean Kim, Jong-Pil Park, Ju Hyung Moon, Eui Hyun Kim, Soohyun Lee, Se Hoon Kim, Cheol Ryong Ku, Eun Jig Lee","doi":"10.1530/JME-21-0070","DOIUrl":"https://doi.org/10.1530/JME-21-0070","url":null,"abstract":"Expression of aberrant microRNA (miRNA) is associated with tumour formation, migration, and invasion. However, there is limited information about the epigenetics of pituitary tumorigenesis. This study investigated the role of miRNA expression during the tumorigenesis of growth hormone (GH)-secreting pituitary tumours. miRNA profiling and real-time PCR were used to analyse the mRNA expression profile in sequential pituitary tissues of a unique animal model with a GH-producing pituitary tumour. Selected miRNAs were further validated in GH-producing cell lines and human pituitary tumour samples. The expression of significantly altered miRNAs and their predicted targets, as detected by microarray, was evaluated by real-time PCR, Western blotting, and immunohistochemistry using samples from mouse models and human pituitary tumours. The effect of miRNAs on tumour proliferation and invasion was examined in GH3 cells using the MTS and Matrigel invasion assays. Among the 14 miRNAs whose expression was significantly changed, miR-216a-5p (fold change = -5.638, P -value = 0.014) and miR-652-3p (fold change = -3.482, P -value = 0.010) were constantly and significantly downregulated. Transfection with mimics of miR-216a-5p and miR-652-3p inhibited GH3 proliferation and invasion, whereas inhibitors promoted them. The direct target genes of miR-216a-5p and miR-652-3p were Jak2 and Prrx1, respectively, which were downregulated in GH3 cells transfected with mimics and in serial pituitary gland tissues, including hyperplasic tissues and tumours of acromegalic animal models and pituitary tumour tissues of acromegalic patients. Downregulated miR-216a-5p and miR-652-3p expression may contribute to tumour progression by targeting JAK2 and PRRX1 on GH-producing pituitary tumours.","PeriodicalId":16570,"journal":{"name":"Journal of molecular endocrinology","volume":"68 1","pages":"51-62"},"PeriodicalIF":3.5,"publicationDate":"2021-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39698098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

MicroRNA-21 enhances estradiol production by inhibiting WT1 expression in granulosa cells. MicroRNA-21通过抑制颗粒细胞中WT1的表达来促进雌二醇的产生。

IF 3.5 4区医学

Journal of molecular endocrinology Pub Date : 2021-11-12 DOI: 10.1530/JME-21-0162

Renée Emily Hilker, Bo Pan, Xiaoshu Zhan, Julang Li

{"title":"MicroRNA-21 enhances estradiol production by inhibiting WT1 expression in granulosa cells.","authors":"Renée Emily Hilker, Bo Pan, Xiaoshu Zhan, Julang Li","doi":"10.1530/JME-21-0162","DOIUrl":"https://doi.org/10.1530/JME-21-0162","url":null,"abstract":"In antral follicles, the transition of proliferative granulosa cells to estradiol-producing is critical for proper oocyte maturation. MicroRNAs are noncoding RNAs that play important roles in ovarian follicular development; however, this has yet to be fully characterized. MicroRNA-21 is significantly higher in granulosa cells isolated from large antral follicles compared to those from small antral follicles. To investigate the function of miR-21, porcine granulosa cells were transfected with miR-21 mimic or miR-21 targeted siRNA. Cells with the miR-21 mimic had higher aromatase expression and estradiol production but decreased WT1 expression. Conversely, cells with the miR-21 siRNA secreted less estradiol and had higher WT1 expression. We hypothesized that miR-21 promotes estradiol production by inhibiting WT1 protein synthesis. We found a potential miR-21 binding site in the 3'UTR of the WT1 transcript and performed a dual-luciferase reporter assay using the WT and mutated 3'UTR. Compared to the negative control, the miR-21 mimic induced a significant decrease in luciferase activity in the WT 3'UTR. This decrease was reversed when the 3'UTR was mutated, suggesting miR-21 targets this site to inhibit WT1 expression. We next transfected porcine granulosa cells with WT1 targeted siRNA and observed a significant increase in aromatase expression and estradiol secretion. We propose that miR-21 represses WT1 expression in granulosa cells to potentially promote aromatase expression and estradiol production. This study offers the first report of a microRNA regulating WT1 expression in granulosa cells and reveals the role of miR-21 in WT1's regulation of estradiol production.","PeriodicalId":16570,"journal":{"name":"Journal of molecular endocrinology","volume":"68 1","pages":"11-22"},"PeriodicalIF":3.5,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39531109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

GPER mediates the IL6/JAK2/STAT3 pathway involved in VEGF expression in swine ovary GCs. GPER介导参与猪卵巢GCs中VEGF表达的IL6/JAK2/STAT3通路。

IF 3.5 4区医学

Journal of molecular endocrinology Pub Date : 2021-11-12 DOI: 10.1530/JME-21-0125

Longfei Xiao, Zihui Wang, Ning Lu, Yanan He, Limin Qiao, Xihui Sheng, Xiaolong Qi, Kai Xing, Yong Guo, Di Chang, Xiangguo Wang, Junjin Zhao, Xiaobin Deng, Hemin Ni, Jian Kang

{"title":"GPER mediates the IL6/JAK2/STAT3 pathway involved in VEGF expression in swine ovary GCs.","authors":"Longfei Xiao, Zihui Wang, Ning Lu, Yanan He, Limin Qiao, Xihui Sheng, Xiaolong Qi, Kai Xing, Yong Guo, Di Chang, Xiangguo Wang, Junjin Zhao, Xiaobin Deng, Hemin Ni, Jian Kang","doi":"10.1530/JME-21-0125","DOIUrl":"https://doi.org/10.1530/JME-21-0125","url":null,"abstract":"Vascular endothelial growth factor (VEGF) plays a pivotal role in angiogenesis in ovaries, particularly during follicular development and ovulation. Interleukin-6 (IL-6) is one of the major pro-inflammatory factors that are involved in the angiogenesis process physiologically and pathologically. Previous studies have shown that 17β-estradiol (E2) stimulates VEGF expression by upregulating hypoxia-inducible factor 1α (HIF-1α) in many cell types, and the high level of E2 causes an inflammatory-like microenvironment before ovulation. However, whether IL-6 signaling is involved in E2-regulating VEGF expression in swine granulosa cells (GCs) is still unknown. In this study, we found the estrogen membrane receptor, G-protein-coupled estrogen receptor 1 (GPER), was expressed in swine GCs, and the expression level of GPER, HIF-1α, and VEGF increased with follicular development. In vitro study showed that E2, ICI182780, and GPER agonist (G1) promoted the expressions of HIF-1α and VEGF in swine GCs, while GPER antagonist (G15) inhibited the stimulating effect of E2 and G1. Meanwhile, G15 inhibited the stimulating effect of E2 and G1 on IL-6 mRNA expression and secretion. Furthermore, IL-6 antibody and AG490 (JAK2/STAT3 inhibitor) attenuated G1-induced HIF-1α and VEGF expression. In conclusion, this study revealed how estrogen-induced HIF-1α and VEGF expressions in swine GCs are mediated through GPER-derived IL-6 secretion leading to JAK2/STAT3 activation.","PeriodicalId":16570,"journal":{"name":"Journal of molecular endocrinology","volume":"68 1","pages":"23-33"},"PeriodicalIF":3.5,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39566936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Bone morphogenetic protein 2 controls steroid-induced osteonecrosis of the femoral head via directly inhibiting interleukin-34 expression. 骨形态发生蛋白2通过直接抑制白细胞介素-34的表达来控制类固醇诱导的股骨头骨坏死。

IF 3.5 4区医学

Journal of molecular endocrinology Pub Date : 2021-10-21 DOI: 10.1530/JME-21-0163

Meng Wang, Hong Sung Min, Haojie Shan, Yiwei Lin, Wenyang Xia, Fuli Yin, Chaolai Jiang, Xiaowei Yu

{"title":"Bone morphogenetic protein 2 controls steroid-induced osteonecrosis of the femoral head via directly inhibiting interleukin-34 expression.","authors":"Meng Wang, Hong Sung Min, Haojie Shan, Yiwei Lin, Wenyang Xia, Fuli Yin, Chaolai Jiang, Xiaowei Yu","doi":"10.1530/JME-21-0163","DOIUrl":"https://doi.org/10.1530/JME-21-0163","url":null,"abstract":"Increased inflammatory response is one of the major characteristics of osteonecrosis of the femoral head (ONFH). We aimed to investigate the function of bone morphogenetic protein 2 (BMP-2)/interleukin (IL)-34 axis in the inflammatory responses of ONFH. The systemic and local expression of BMPs in ONFH patients was detected by qRT-PCR and ELISA. In vitro osteoclast differentiation and ONFH mouse models, induced by 20 mg/kg methylprednisolone through i.m. injection, were established using WT and BMP-2-/- mice to explore the regulatory role of BMP-2 in pro-inflammatory responses and bone defects of ONFH. IL-34 expression and function were examined in vitro and in vivo through qRT-PCR, tartrate-resistant acid phosphatase (TRAP) staining, and gene knockout. The systemic and local expression of BMPs was elevated in ONFH patients. BMP-2 reduced the production of pro-inflammatory cytokines and inhibited the differentiation of osteoclasts. Mechanistically, BMP-2 inhibited osteoclasts formation through suppressing IL-34 expression and then promoted bone repair and alleviated ONFH. In conclusion, our study reveals that BMP-2 inhibits inflammatory responses and osteoclast formation through downregulating IL-34.","PeriodicalId":16570,"journal":{"name":"Journal of molecular endocrinology","volume":"68 1","pages":"1-9"},"PeriodicalIF":3.5,"publicationDate":"2021-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39464978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

25 years of ERβ: a personal journey. 25年的ERβ:一个人的旅程。

IF 3.5 4区医学

Journal of molecular endocrinology Pub Date : 2021-10-15 DOI: 10.1530/JME-21-0121

Margaret Warner, Xiaotang Fan, Anders Strom, Wanfu Wu, Jan-Åke Gustafsson

{"title":"25 years of ERβ: a personal journey.","authors":"Margaret Warner, Xiaotang Fan, Anders Strom, Wanfu Wu, Jan-Åke Gustafsson","doi":"10.1530/JME-21-0121","DOIUrl":"https://doi.org/10.1530/JME-21-0121","url":null,"abstract":"Summary: After the discovery of ERβ, a novel role for dihydrotestosterone (DHT) in estrogen signaling was revealed. Instead of just being a better androgen, DHT was found to be a precursor of the ERβ agonist 5α-androstane-3β, 17β-diol (3βAdiol), an estrogen which does not require aromatase for its synthesis. ERβ was found to oppose androgen signaling and thus is a potential target for treatment of prostate cancer. ERβ was also found to have effects that were independent of androgen signaling, particularly in the CNS. Although in rodent models of neurodegenerative diseases (Parkinson's disease, multiple sclerosis, and Alzheimer's disease), ERβ agonists are very effective in relieving symptoms and improving pathologies, this has not proven to be the case in humans. In this review we will focus on the main differences in ERβ signaling between rodents and humans and will make the point that a very important difference between the two species is in the splice variants which are expressed in humans and not rodents. The main conclusion at this point is that before we think of using ERβ agonists clinically, much more work on ERβ signaling in the human or in primates needs to be done.","PeriodicalId":16570,"journal":{"name":"Journal of molecular endocrinology","volume":"68 1","pages":"R1-R9"},"PeriodicalIF":3.5,"publicationDate":"2021-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39435920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Characterization of a mutated KCNJ5 gene, G387R, in unilateral primary aldosteronism. KCNJ5基因G387R在单侧原发性醛固酮增多症中的突变特征

IF 3.5 4区医学

Journal of molecular endocrinology Pub Date : 2021-09-28 DOI: 10.1530/JME-20-0282

Jeff S Chueh, Kang-Yung Peng, Vin-Cent Wu, Shuo-Meng Wang, Chieh-Kai Chan, Yung-Ming Chen, Yi-Yu Ke, Chien-Yuan Pan, Hung-Wei Liao

{"title":"Characterization of a mutated KCNJ5 gene, G387R, in unilateral primary aldosteronism.","authors":"Jeff S Chueh, Kang-Yung Peng, Vin-Cent Wu, Shuo-Meng Wang, Chieh-Kai Chan, Yung-Ming Chen, Yi-Yu Ke, Chien-Yuan Pan, Hung-Wei Liao","doi":"10.1530/JME-20-0282","DOIUrl":"https://doi.org/10.1530/JME-20-0282","url":null,"abstract":"Somatic mutation in the KCNJ5 gene is a common driver of autonomous aldosterone overproduction in aldosterone-producing adenomas (APA). KCNJ5 mutations contribute to a loss of potassium selectivity, and an inward Na+ current could be detected in cells transfected with mutated KCNJ5. Among 223 unilateral primary aldosteronism (uPA) individuals with a KCNJ5 mutation, we identified 6 adenomas with a KCNJ5 p.Gly387Arg (G387R) mutation, previously unreported in uPA patients. The six uPA patients harboring mutant KCNJ5-G387R were older, had a longer hypertensive history, and had milder elevated preoperative plasma aldosterone levels than those APA patients with more frequently detected KCNJ5 mutations. CYP11B2 immunohistochemical staining was only positive in three adenomas, while the other three had co-existing multiple aldosterone-producing micronodules. The bioinformatics analysis predicted that function of the KCNJ5-G387R mutant channel could be pathological. However, the electrophysiological experiment demonstrated that transfected G387R mutant cells did not have an aberrantly stimulated ion current, with lower CYP11B2 synthesis and aldosterone production, when compared to that of the more frequently detected mutant KCNJ5-L168R transfected cells. In conclusion, mutant KCNJ5-G387R is not a functional KCNJ5 mutation in unilateral PA. Compared with other KCNJ5 mutations, the observed mildly elevated aldosterone expression actually hindered the clinical identification of clinical unilateral PA. The KCNJ5-G387R mutation needs to be distinguished from functional KCNJ5 mutations during genomic analysis in APA evaluation because of its functional silence.","PeriodicalId":16570,"journal":{"name":"Journal of molecular endocrinology","volume":"67 4","pages":"203-215"},"PeriodicalIF":3.5,"publicationDate":"2021-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39385844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1