Ryotaro Sakakibara, Masaru Takeuchi, A. Ichikawa, K. Ohara, M. Nakajima, Y. Hasegawa, T. Fukuda
{"title":"On-chip fabrication of bio-degradable microstructures","authors":"Ryotaro Sakakibara, Masaru Takeuchi, A. Ichikawa, K. Ohara, M. Nakajima, Y. Hasegawa, T. Fukuda","doi":"10.1109/MHS.2015.7438341","DOIUrl":"https://doi.org/10.1109/MHS.2015.7438341","url":null,"abstract":"In this paper, we propose a method of cell assembly on microstructures of a biodegradable material for the regeneration of living tissue. The adhesion of fabricated biodegradable structures on the substrate was one of the important challenges. The material was tested trypsin EDTA-dipped Dialysis membrane. The results show that one side of the microstructure after 2 minutes was peeled off, but I did not peel off the other side.","PeriodicalId":165544,"journal":{"name":"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"206 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122465421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multiple-reagent layer laden high-speed micro-bubbles in extended two-dimensional microchip","authors":"Y. Hamano, T. Kambayashi, Y. Yamanishi","doi":"10.1109/MHS.2015.7438236","DOIUrl":"https://doi.org/10.1109/MHS.2015.7438236","url":null,"abstract":"In conventional chemical analysis methods, cohesion and ununiformed concentration within the mixed solution tend to be occurred during the reagent mixing process, which causes the increase of reagent consumption and process time. In the past, the authors have invented a device called “bubble injection knife”. In this device, high-speed micro-bubbles are ejected piercing through a reagent interface by discharging from a Cu wire, transporting a thin reagent membrane formed on the surface of the bubble due to its adsorption force. In addition, it is known that micro-bubbles contract and collapse. In this study, the authors designed a device using the properties of micro-bubbles and the bubble injection knife, which purpose is to reduce reagent consumption and enable uniform mixing in chemical analysis. This device is a micro-chip which is designed to produce micro-bubbles by electric discharge from a “bubble kife” embedded in the micro-channel and make them penetrate through reagent laminar flows in order to form reagent layers on the bubble's surface. Moreover, the mixing of these layers using the contraction force of the bubbles is expected. In the experiments that were conducted, micro-bubbles were produced in the micro-channel from the embedded bubble knife. This technology is expected to improve chemical analysis methods to overcome ununiformed concentration within the mixed solution, and also it is expected to contribute to the bio-medical reagent injection with control of reaction time.","PeriodicalId":165544,"journal":{"name":"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122504490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sakiko Enomoto, Yuya Yajima, Yuki Watabe, M. Yamada, K. Furusawa, M. Seki
{"title":"One-step microfluidic spinning of collagen microfibers and their application to cell cultivation","authors":"Sakiko Enomoto, Yuya Yajima, Yuki Watabe, M. Yamada, K. Furusawa, M. Seki","doi":"10.1109/MHS.2015.7438257","DOIUrl":"https://doi.org/10.1109/MHS.2015.7438257","url":null,"abstract":"Here we propose a simple but efficient method to produce collagen hydrogel microfibers using a microfluidic spinning system and a formation technique of collagen gels in a phosphate buffer. An aqueous solution of type I collagen was introduced into the microfluidic devices together with a phosphate buffer and distilled water. Parallel laminar flow was stably formed in the microchannel, and the collagen solution was transformed into microfibers with a uniform diameter. We examined factors affecting the fiber diameter and successfully obtained microfibers with a diameter from ~8 to ~30 μm. In addition, we confirmed that cells adhered on the surface of the microfibers and proliferated after culturing for several days. The presented method is advantageous because it enables the production of collagen microfibers under a wet condition without using complicated operations, organic solvents, or crosslinking reagents.","PeriodicalId":165544,"journal":{"name":"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"103 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123023025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. C. Purbarani, Qurrotin A'yunina Moa, G. Jati, M. A. Ma'sum, H. A. Wisesa, W. Jatmiko
{"title":"Implementation of grid mapped robot planning algorithm in a continuous map for fire fighting robot","authors":"S. C. Purbarani, Qurrotin A'yunina Moa, G. Jati, M. A. Ma'sum, H. A. Wisesa, W. Jatmiko","doi":"10.1109/MHS.2015.7438292","DOIUrl":"https://doi.org/10.1109/MHS.2015.7438292","url":null,"abstract":"Fire-fighting robot is still one of the fields in robotic competitions held these days. This paper is aimed to see the implementation of the Markov Decision Planning (MDP) problem in a fire-fighting robot's navigation. The MDP algorithm evolves planning of the actions the robot should take according to the policy. This planning is mapped into a grid map. Yet in the implementation, this planning is applied in a continuous map. Using a fire-fighting robot the succession of this planning implementation is undertaken. The result shows that the implementation of grid mapped in a continuous map yields significant impacts that lead the MDP to be able to solve the limitation of wall following algorithm. This algorithm is also applied in the real autonomous mobile robot.","PeriodicalId":165544,"journal":{"name":"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123138842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Gaze estimation driven solution for interacting children with ASD","authors":"Haibin Cai, Xiaolong Zhou, Hui Yu, Honghai Liu","doi":"10.1109/MHS.2015.7438336","DOIUrl":"https://doi.org/10.1109/MHS.2015.7438336","url":null,"abstract":"This paper investigates gaze estimation solutions for interacting children with Autism Spectrum Disorders (ASD). Previous research shows that satisfactory accuracy of gaze estimation can be achieved in constrained settings. However, most of the existing methods can not deal with large head movement (LHM) that frequently happens when interacting with children with ASD scenarios. We propose a gaze estimation method aiming at dealing with large head movement and achieving real time performance. An intervention table equipped with multiple sensors is designed to capture images with LHM. Firstly, reliable facial features and head poses are tracked using supervised decent method. Secondly, a convolution based integer-differential eye localization approach is used to locate the eye center efficiently and accurately. Thirdly, a rotation invariant gaze estimation model is built based on the located facial features, eye center, head pose and the depth data captured from Kinect. Finally, a multi-sensor fusion strategy is proposed to adaptively select the optimal camera to estimate the gaze as well as to fuse the depth information of the Kinect with the web camera. Experimental results showed that the gaze estimation method can achieve acceptable accuracy even in LHM situation and could potentially be applied in therapy for children with ASD.","PeriodicalId":165544,"journal":{"name":"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121652453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Japanese syllabary identification using myoelectric potential of neck muscles","authors":"Kentaro Suzuki, Y. Hasegawa","doi":"10.1109/MHS.2015.7438312","DOIUrl":"https://doi.org/10.1109/MHS.2015.7438312","url":null,"abstract":"The purpose of this paper is to achieve Japanese syllabary identification without using speech signals. For this purpose, I put an array electrode on the anterior surface of neck and measure BEP signals and I propose a method for classifying Japanese syllabary using BEP signals with SVM. As a first step, we conducted an experiment to identify 10 Japanese syllabary (46 kinds) and rest state. As a result, the average of identification accuracy is 96.6% and least 80%.","PeriodicalId":165544,"journal":{"name":"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132545372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ryosaku Sakamoto, Yuya Matsuse, M. Nagai, T. Shibata
{"title":"Development and characterization of dielectrophoretic probe array for non-invasive massively parallel manipulation of single cells","authors":"Ryosaku Sakamoto, Yuya Matsuse, M. Nagai, T. Shibata","doi":"10.1109/MHS.2015.7438340","DOIUrl":"https://doi.org/10.1109/MHS.2015.7438340","url":null,"abstract":"Cell manipulation system is an essential tool for reconstruction of cellular microenvironment that leads to high-throughput function analysis. Our aim is the development of dielectrophoretic probe array for non-invasive and massively parallel manipulation of single cells. In this paper, we report fabrication of pairs of polymer pillars and formation of a sacrifice layer for three-dimensional electrodes. On dielectric-covered electrodes, dielectrophoretic forces were generated by the application of AC voltage. Cells were captured on the electrodes in a parallel manner. Dielectrophoretic manipulation with coated-electrodes provides a way to manipulate cells non-invasively.","PeriodicalId":165544,"journal":{"name":"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132381242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Farahat, G. Sathi, Hiroaki Taketa, M. Oshima, T. Kuboki, T. Matsumoto
{"title":"Highly dynamic self-organizing SMG morphogenesis in 3D culture","authors":"M. Farahat, G. Sathi, Hiroaki Taketa, M. Oshima, T. Kuboki, T. Matsumoto","doi":"10.1109/MHS.2015.7438259","DOIUrl":"https://doi.org/10.1109/MHS.2015.7438259","url":null,"abstract":"Biological tissues have specific cell patterns and well-organized structures which result from various organogenesis processes. Among which is branching morphogenesis which is the key developmental processes for many glandular tissues including lungs, kidney, salivary gland and liver. Many approaches were suggested to regenerate salivary gland tissue in vitro. Here, we developed a 3D culture technique to study and recapitulate the dynamic forces generated during SMG organogenesis by utilizing time lapse live imaging to monitor the cellular migration and reorganization process of both epithelium and mesenchyme cells.","PeriodicalId":165544,"journal":{"name":"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"78 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132382978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Melo, P. Alsina, C. Dórea, Kassio J. S. Eugenio
{"title":"Influence of different gait trajectories in an lower limb active orthosis performance based on user metabolic cost and motors usage","authors":"N. Melo, P. Alsina, C. Dórea, Kassio J. S. Eugenio","doi":"10.1109/MHS.2015.7438253","DOIUrl":"https://doi.org/10.1109/MHS.2015.7438253","url":null,"abstract":"In active orthoses for paraplegic users, it is essential that the devices replicate the user's leg movements. This is usually performed through joint position control, where each orthosis joint follows a specific angle trajectory as a reference. The most commonly used approach for generating this required references is to create a set of average joint trajectories from a gait database, and use them as a set of default references. Although recent approaches shown improvement, the influence of a user oriented gait trajectory to the orthosis general performance is still unknown. Nevertheless, due to the complexity of human gait system and individual body reaction when wearing an active lower limb orthosis, the efficacy and performance of such devices are still in current analysis. Taking the metabolic effort realized during gait and the electric power used by the motors in consideration, this work presents a performance comparison between different types of trajectories followed by the orthosis. The metabolic effort realized by the user will be obtained through the measurement of the heart beat rate. The experiments were realized in Ortholeg orthosis with a healthy subject. The main contribution of this work is to provide information regarding the influence of different gait types an active lower limb orthosis performance, allowing a better gait trajectory planning that can be used for daily walk or for rehabilitations programs.","PeriodicalId":165544,"journal":{"name":"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134206966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Automation of an on-chip cell mechanical characterization system for stiffness evaluation","authors":"Bilal Turan, S. Sakuma, Lin Feng, F. Arai","doi":"10.1109/MHS.2015.7438327","DOIUrl":"https://doi.org/10.1109/MHS.2015.7438327","url":null,"abstract":"We present an automation system for on-chip cell mechanical characterization system for evaluating the stiffness of oocytes. A high frequency visual feedback control system is proposed. Using simple yet effective image analysis algorithms, the high frequency visual feedback is able to improve the response of the cell manipulation, the throughput of the system is improved consequently. To measure the stiffness of the oocytes, a microfluidic chip with magnetically driven on-chip probe and a thin beam based force sensor is used [1].","PeriodicalId":165544,"journal":{"name":"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"91 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120982427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}