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Testing the efficacy of phytohormones of microbial origin in promoting plant growth 微生物来源的植物激素促进植物生长的功效试验

Journal of Microbiology and Biotechnology Research Pub Date : 2017-03-29 DOI: 10.24896/JMBR.2017711

V. Mahalakshmi, Ditisha Bhagawati

{"title":"Testing the efficacy of phytohormones of microbial origin in promoting plant growth","authors":"V. Mahalakshmi, Ditisha Bhagawati","doi":"10.24896/JMBR.2017711","DOIUrl":"https://doi.org/10.24896/JMBR.2017711","url":null,"abstract":"Mustard Is an Important Oilseed Which Is Used For many Purposes Like Culinary, Religious And Cultural Purposes By Humanity Since Time Immemorial. To Increase The Production of Mustard, Phytohormones Can Be Used Which Are Pro1duced With The Help Of Microorganisms. Phytohormones are the signaling or organic compound which helps in the development and growth of plant. Rhizobium and Bacillus sp were used to produce phytohormones which are isolated from different sources and investigated for their ability to produced auxin and cytokinin in PM2 and Czapek medium respectively. Thin layer chromatography and High performance liquid chromatography were performed to detect and quantify the amount of hormones in the production medium. Rhizobium produced the highest auxin (0.087mg/l) and cytokinin (0.047mg/ml) in the production medium. Then these microbiologically synthesized auxin and cytokinin were treated with mustard seeds to check their ability in promoting growth development. Auxin produced from microorganisms comparatively showed the fastest shoot development (7.9cm length) and leaf (length-1.8cm and width-1.9cm) than cytokinin and control.","PeriodicalId":16482,"journal":{"name":"Journal of Microbiology and Biotechnology Research","volume":"5 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2017-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87848456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Production of Pullulan from a high yielding strain of Aureobasidium pullulans in non-stirred flask-type fermentation system 一株高产普鲁兰小孢子菌在非搅拌烧瓶式发酵系统中生产普鲁兰

Journal of Microbiology and Biotechnology Research Pub Date : 2017-03-29 DOI: 10.24896/JMBR.2017715

R. Singh, R. Gaur, S. Bansal, Farrukh Jamal, P. Pandey, S. Tiwari, S. Sarsaiya, Saket Mishra, N. Chaturvedi, D. P. Singh, M. K. Gaur, Father G. Vazhan Arasu

引用次数: 8

Comparative Evaluation of Computational and Experimental Analysis of Polyphenol Oxidase from Cocoa (Theobroma cacao L.) 可可多酚氧化酶的计算分析与实验分析比较

Journal of Microbiology and Biotechnology Research Pub Date : 2017-03-29 DOI: 10.24896/JMBR.2017714

D. M. Sanni, T. Fatoki, O. Omotoyinbo

{"title":"Comparative Evaluation of Computational and Experimental Analysis of Polyphenol Oxidase from Cocoa (Theobroma cacao L.)","authors":"D. M. Sanni, T. Fatoki, O. Omotoyinbo","doi":"10.24896/JMBR.2017714","DOIUrl":"https://doi.org/10.24896/JMBR.2017714","url":null,"abstract":"Cocoa (Theobroma cacao L.) is a cash crop of huge economic significance in the world and the key raw material for chocolate manufacturing. Polyphenol oxidases (PPOs) are copper containing oxidoreductases that catalyze the hydroxylation and oxidation of phenolic compounds in the presence of molecular oxygen. The PPO presence and activity during fermentation and drying of cocoa beans is responsible for the development of flavor precursors. In this study, bioinformatics analysisof PPO from cocoa was done using standard bioinformatics tools such as Blastp, Hmmer, ClustalO, OMABrowser, EMBOSS and Swissmodel. The result showed that cocoa PPO homologs include Citrus sinensis, Populus euphratica, Gossypium raimondii, Litchi chinensis, Dimocarpuslongan, Canarium album, while the orthologsobtainedinclude Sorghum bicolor, Zea may, Manihot esculenta and Vitis vinifera among others.PPO of Solanum lycopersicum (tomato) was found to be a distant homolog of cocoa PPO, and that this evolutionary relationship implicated Witches’ Broom Disease (WBD). The computed physicochemical properties were in alignment with the experimental results. Keywords: Polyphenol Oxidase, Cocoa, Theobroma cacao L., Computational Analysis, Experimental Analysis.","PeriodicalId":16482,"journal":{"name":"Journal of Microbiology and Biotechnology Research","volume":"126 1","pages":"18-25"},"PeriodicalIF":0.0,"publicationDate":"2017-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88137975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

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