Testing the efficacy of phytohormones of microbial origin in promoting plant growth

Mustard Is an Important Oilseed Which Is Used For many Purposes Like Culinary, Religious And Cultural Purposes By Humanity Since Time Immemorial. To Increase The Production of Mustard, Phytohormones Can Be Used Which Are Pro1duced With The Help Of Microorganisms. Phytohormones are the signaling or organic compound which helps in the development and growth of plant. Rhizobium and Bacillus sp were used to produce phytohormones which are isolated from different sources and investigated for their ability to produced auxin and cytokinin in PM2 and Czapek medium respectively. Thin layer chromatography and High performance liquid chromatography were performed to detect and quantify the amount of hormones in the production medium. Rhizobium produced the highest auxin (0.087mg/l) and cytokinin (0.047mg/ml) in the production medium. Then these microbiologically synthesized auxin and cytokinin were treated with mustard seeds to check their ability in promoting growth development. Auxin produced from microorganisms comparatively showed the fastest shoot development (7.9cm length) and leaf (length-1.8cm and width-1.9cm) than cytokinin and control.