{"title":"Direct and Sunlight-Induced Photosensitizing Antibacterial Activities of Curcuma longa Extract","authors":"Kayonika Bhadra, Ananya Basu, Bhaskar Narayan Choudhuri, Partha Guchhait, Arup Kumar Dawn, Satadal Das","doi":"10.36349/easjbg.2024.v06i03.002","DOIUrl":"https://doi.org/10.36349/easjbg.2024.v06i03.002","url":null,"abstract":"Curcuma longa is a widely used plant in folk medicine. Here, we assessed the antimicrobial activities of the ethanolic extract of Curcuma longa with particular emphasis on its combined effect with sunlight, as the extract can produce fluorescence in the presence of UV rays. Curcumin is one of the most active ingredients of Curcuma longa. The ethanolic extract of Curcuma longa when added to lawn cultures of gram-positive and gram-negative bacteria and exposed to sunlight the growth of bacteria was remarkably reduced. Micro broth dilution assay also showed decreased MIC values after exposure to sunlight with MSSA. Our results encourage the potential use of Curcuma longa as an antibacterial product.","PeriodicalId":164482,"journal":{"name":"EAS Journal of Biotechnology and Genetics","volume":"9 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140974860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Perturbation of Biological Markers in COVID-19 Positive Type-2 Diabetic Subjects (Delta and Omicron Variant Cases) in Pointe-Noire","authors":"Freddy Saturnin Pouki, Luc Magloire Anicet Boumba, Parode Ragive Takale, Charley Elenga- Bongo, Aladin Atandi Batchy, Rebecca Frédérique Dussaud, Colombe Sagesse Luzayisu","doi":"10.36349/easjbg.2024.v06i03.001","DOIUrl":"https://doi.org/10.36349/easjbg.2024.v06i03.001","url":null,"abstract":"Introduction: The SARS-CoV-19 pandemic affected the whole world, with a particularly high and severe incidence in patients with co-morbidities such as type 2 diabetes (T2DM). The SARS-CoV-2 variants, Delta and Omicron, posed an additional challenge in the management of these patients because of their virulence and high transmissibility. Objective: To assess the disturbance of certain biomarkers in COVID-19-positive T2DM patients in Pointe-Noire. Methods: We performed a descriptive cross-sectional study on a cohort of 206 type 2 diabetic patients affected by COVID-19. Blood samples were taken for analysis of biomarkers (CBC, ESR, CRP, GLY, and HbA1c, lipid profile, urea and creatinine). RNA was extracted from nasopharyngeal samples and PCR was performed to determine the presence of Delta and Omicron variants. Results: The mean age of our patients was 56.33 ± 12 years. The Delta variant was the most common 132 (64.08%), followed by the other variants 57 (27.67%) and Omicron 17 (8.25%). We observed significant disturbances (p<0.05) in biomarkers as a function of Delta and Omicron variants concerning: TC, TG, HDL, LDL, DDI, VS, CRP, GLY, and HbA1c. Conclusion: We identified the Delta variant as the most frequent in the diabetic population studied. Almost all biological markers studied were disrupted during COVID-19 infection in the type 2 diabetic population.","PeriodicalId":164482,"journal":{"name":"EAS Journal of Biotechnology and Genetics","volume":"52 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141008315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nikita Parui, Arijit Halder, P. Guchhait, Bhaskar Narayan Chaudhuri, Satadal Das
{"title":"Candida spp. are Largely Alien in Beach Mycosands","authors":"Nikita Parui, Arijit Halder, P. Guchhait, Bhaskar Narayan Chaudhuri, Satadal Das","doi":"10.36349/easjbg.2024.v06i01.003","DOIUrl":"https://doi.org/10.36349/easjbg.2024.v06i01.003","url":null,"abstract":"Only recently microbial impurity of beach sand has become an essential issue, particularly during the COVID-19 and in the post-Covid period when a large number of beachgoers are infected not only by the virus but also with other microbes. Besides the bacterial and viral microbes, fungal contamination of beach sand has also been studied in detail in the recent past. Although few Candida spp. have been isolated from the beach sand, the growths of common pathogenic Candida spp. like Candida albicans has not been studied so far. In this study, we used two species of pathogenic Candida – Candida albicans and Candida parapsilosis to observe their growth patterns in sea sand. Growths of both species were inhibited in sea sand. It eliminates the possibility of long survival of these species in sea sand and thus their existence mainly appears as surface contamination.","PeriodicalId":164482,"journal":{"name":"EAS Journal of Biotechnology and Genetics","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140492316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rana R. El Sadda, Heba O. El Sharawy, Omnia H. Gohar, Elhossein A. Moawed, Wael S. El– Tohamy
{"title":"Geographical Variations in Liver Cancer Epidemiology and Diagnosis in the Damietta Governorate, Egypt: An Observational Study","authors":"Rana R. El Sadda, Heba O. El Sharawy, Omnia H. Gohar, Elhossein A. Moawed, Wael S. El– Tohamy","doi":"10.36349/easjbg.2023.v05i05.001","DOIUrl":"https://doi.org/10.36349/easjbg.2023.v05i05.001","url":null,"abstract":"Background: Liver cancer is a major health problem in Egypt. Understanding its epidemiology can help identify high-risk groups and inform prevention strategies. Objective: To investigate regional and occupational variations in liver cancer characteristics and diagnoses in Damietta governorate, Egypt. Methods: An observational study was conducted using data from the Damietta Cancer Institute in 2021. 128 case diagnosed as Liver cancer were analyzed based on age, location, occupation, cancer stage, provisional diagnosis, and sex. Results: Primary liver cancer was more prevalent in younger individuals while metastatic cancer dominated in older age groups. Certain areas exhibited higher primary cancer rates than others. Provisional diagnoses varied by occupation and location. Male patients presented with more advanced stages and distant metastasis compared to females. Conclusions: Geographical and occupational disparities exist in liver cancer profiles. Sex differences were observed in disease presentation. Robust studies are needed to establish causal relationships and explore underlying disease mechanisms. Understanding regional cancer patterns can help prioritize preventive efforts and develop targeted screening programs.","PeriodicalId":164482,"journal":{"name":"EAS Journal of Biotechnology and Genetics","volume":"252 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139161121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sulagna Naskar, D. Chatterjee, Banhishikha Singh, Krishnendu Paira, Satadal Das
{"title":"A Study on IFN-γ and IL-10 Gene Expression Changes in Gallus gallus Embryo Infected with Pseudomonas aeruginosa and Its Possible Restoration by Bakreshwar Hot Spring Water","authors":"Sulagna Naskar, D. Chatterjee, Banhishikha Singh, Krishnendu Paira, Satadal Das","doi":"10.36349/easjbg.2023.v05i03.004","DOIUrl":"https://doi.org/10.36349/easjbg.2023.v05i03.004","url":null,"abstract":"Bakreswar hot spring is a naturally occurring hot spring located at Birbhum district in West Bengal, India. The surface temperatures of the hot springs varies between 35°C to 66°C. The water of hot spring is alkaline with pH 9 and has curative properties. Pseudomonas aeruginosa is an important pathogen causing hospital acquired infections and in this study we used multi-drug resistance (MDR) isolate. The motive of the study is to compare the IL-10 and IFN-γ gene expression changes in Gallus gallus domesticus embryo infected with Pseudomonas aeruginosa by using the Bakreswar hot spring water.The curative properties of the hot spring water collected from Agnikund (65°C) was studied on 14thdays old chick eggs infected with Pseudomonas aeruginosa. After harvesting and allantoic fluid collection, RNA extraction was done and cDNA synthesis and RT-PCR were performed. After infection of the chick embryo with MDR Pseudomonas aeruginosa, both IL-10 and IFN-Ƴ gene expressions were increased, but in the curative sets those were challenged with Bakreswar hot spring water IL-10 and IFN- γ both gene expressions were decreased. From this study we may conclude that hot spring water of Bakreswar has antimicrobial activity against Pseudomonas aeruginosa due to which it decreses the cytokine gene expressions.","PeriodicalId":164482,"journal":{"name":"EAS Journal of Biotechnology and Genetics","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125350232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Moukouma Constantin, Poaty Henriette, Zohoncon Theodora M, Mounkassa Juanat Vitek, Silou Massamba Jacques François, Poaty Guy Emergence, Odzebé Anani Severin Wencesl
{"title":"Male Infertility in Brazzaville: Prevalence and Sperm Abnormalities Patterns","authors":"Moukouma Constantin, Poaty Henriette, Zohoncon Theodora M, Mounkassa Juanat Vitek, Silou Massamba Jacques François, Poaty Guy Emergence, Odzebé Anani Severin Wencesl","doi":"10.36349/easjbg.2023.v05i03.006","DOIUrl":"https://doi.org/10.36349/easjbg.2023.v05i03.006","url":null,"abstract":"Background: Male infertility affects 7% of males worldwide (i.e. 30 million of men). The diagnosis of the condition is based firstly on the semen analysis which can show various types of abnormalities. In Congo, data on male infertility are poor. This study aimed to determine the prevalence of male infertility in Brazzaville and to provide the profile of the semen parameters from a cohort of infertile men living at Brazzaville. Material and Methods: We studied semens of three hundred and forty-four (344) congolese men affected by infertility. The work was based on medical data to research medical history and the semen to performed the spermogram and spermocytogram in way to precise the sperm parameters, Results: The prevalence of male infertility found was 9% and the average age was 39 years old. Medical history highligts mostly the notion of chronic infection, varicoceles and surgery of the genital tract. The most identified sperm abnormalities were moderate asthenozoospermia followed by severe oligoasthenozoospermia and azoospermia. Conclusion: The prevalence shows that male infertility is not rare in Brazzaville. As the condition seemed to be related to the infectious, tumoral and professional causes, a complete investigation (based on sperm analysis, biochemical markers and genetic tests) is needed to clarify the etiologies.","PeriodicalId":164482,"journal":{"name":"EAS Journal of Biotechnology and Genetics","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132332361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Principal Component Analysis and Genetic Divergence Studies for Yield and Yield-Related Trait of Groundnut (Arachis hypogaea L.) Genotypes at Pawe North Western Ethiopia","authors":"Desalegn Nigatu Habite","doi":"10.36349/easjbg.2023.v05i03.005","DOIUrl":"https://doi.org/10.36349/easjbg.2023.v05i03.005","url":null,"abstract":"A study was conducted on sixty-four (64) groundnut genotypes at Pawe northwestern part of Ethiopia. And the analysis of variance revealed the existence of significant variation among the tested groundnut genotypes. Cluster analysis showed the existence of five (5) divergent groups and the maximum inter-cluster distance was observed between cluster (II) and cluster (V) D2=1419.77 and the shortest inter-cluster squared distance was found between cluster (I) and Cluster (III) D2=247.23. The first four principal components for (PCs) accounted for 70.03% of the total variation. Overall, the results of the current study depicted the presence of sufficient genetic diversity existed among tested groundnut genotypes for further use in breeding programs. However, a one-season experiment will not realize variability because quantitative traits are polygenic and highly influenced by the environment. So, further experiments must be conducted over location and seasons.","PeriodicalId":164482,"journal":{"name":"EAS Journal of Biotechnology and Genetics","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131401807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ritojo Basu, Teesta Bhowmick, Rittika Singh, B. Chaudhuri, P. Guchhait, Satadal Das
{"title":"Action of Ethanolic Extract of Holerrhena pubescens on Bacterial Strains","authors":"Ritojo Basu, Teesta Bhowmick, Rittika Singh, B. Chaudhuri, P. Guchhait, Satadal Das","doi":"10.36349/easjbg.2023.v05i03.002","DOIUrl":"https://doi.org/10.36349/easjbg.2023.v05i03.002","url":null,"abstract":"The need to screen efficient, safe, affordable, and readily available antimicrobial agents from various medicinal plants, for their potential antimicrobial activity has become critical in light of the rapid development of microbial resistance against chemotherapeutic drugs (mainly antibiotics). Despite notable advances in the biological and phytochemical evaluation in recent years, comprehensive reviews of Holarrhena pubescens are limited. The main objective of this study is to evaluate the antimicrobial activities of this plant extract. One significant use of plant extracts can be in treating bacterial infections and develop potential antibacterial agents against multi drug resistant (MDR) and susceptible bacteria. If antimicrobial activities are present, these extracts will provide useful information for discovering new compounds with better activity and more effectiveness against resistant and susceptible bacteria responsible for infections than currently available. The ethanolic extract of leaves of Holarrhena pubescens was prepared and tested against multidrug resistant (MDR) and American type culture collection strains of bacteria. We have obtained two types of results in this experiment. The extract showed antimicrobial properties against Staphylococcus aureus (ATCC 23235), Escherichia coli (MDR) and Acinetobacter (MDR), while, it showed no antimicrobial activities along with some growth promoting role against Escherichia coli (ATCC 25922), and Klebsialla pneumoniae (MDR), which may be due to chemicals other than secondary metabolites.","PeriodicalId":164482,"journal":{"name":"EAS Journal of Biotechnology and Genetics","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127542615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arkadeep Roy, B. Chaudhuri, P. Guchhait, Satadal Das
{"title":"Antimicrobial Activity Avicennia marina Flowers","authors":"Arkadeep Roy, B. Chaudhuri, P. Guchhait, Satadal Das","doi":"10.36349/easjbg.2023.v05i03.001","DOIUrl":"https://doi.org/10.36349/easjbg.2023.v05i03.001","url":null,"abstract":"Antimicrobial drug resistance is gradually increasing throughout the globe, and there is practically no newer antimicrobial agent coming soon. Plant secondary metabolites are well known for their various biological activities, and there are few studies targeting mangrove plants. Thus, in this study, we aim to assess the effectiveness of the ethanolic extract of the flowers of Avicennia marina, a commonly found mangrove species in coastal regions, against multi-drug resistant (MDR) microorganisms. The study focused on evaluating the extract's antibacterial activity against Staphylococcus aureus (ATCC 29213), Escherichia coli (ATCC 25922), Escherichia coli (MDR), Klebsiella sp. (MDR), Acinetobacter sp. (MDR), Pseudomonas sp. (MDR). The results of the study demonstrated that the flower extracts of Avicennia marina exhibited noteworthy antimicrobial properties against all the tested MDR microorganisms. The plant contains several bioactive compounds, such as flavonoids, tannins, and alkaloids, which are believed to contribute to the observed antimicrobial effects. These findings indicate that Avicennia marina flowers could serve as a valuable natural source for developing antimicrobial agents that could combat drug-resistant bacterial infections. However, future studies should focus on evaluating the efficacy and safety of this extract in live organisms (in vivo) to determine its potential as a new drug for treating resistant infections.","PeriodicalId":164482,"journal":{"name":"EAS Journal of Biotechnology and Genetics","volume":"181 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121271094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impacts of Gama Irradiations on the Development New Mutants of Stevia rebaudiana Bertoni","authors":"R. Abdullateef, Z. Zainuddin, M. Osman","doi":"10.36349/easjbg.2023.v05i02.001","DOIUrl":"https://doi.org/10.36349/easjbg.2023.v05i02.001","url":null,"abstract":"Stevia rebaudiana Bertoni is known for its production of sweet glycosides, an alternative to sugar. The cultivation of stevia in Malaysia is hampered because- it produces flowers at an early stage under Malaysia's photoperiod condition, thus leading to poor leaf yield for sweetener extraction. Additionally, there has been low breeding work for suitable cultivars. Thus, the impact of Gama irradiation in developing new Stevia mutants was studied. Seeds of MS012 were collected and exposed to gamma irradiation using a gamma cell in UKM, Malaysia to determine the LD50. The seeds were packed into 21 parts; one part served as the control and the others were subjected to varying gamma irradiation grays of 100 to 2000 grays. Irradiated seeds were sown for germination under the red light for 15 days. The LD50 was determined by plotting a simple regression graph of plant height against irradiation grays. Results revealed LD50 was 55 grays. The number of germinated seeds differed with respect to doses. Genetic analysis showed four types of chlorophyll mutants were induced: Albina, Chlorina, Xantha and Xantha-Viridis. Frequency and spectrum analysis revealed that Chlorina has the highest occurrence at 33.03%. Two classical novelties: (i) M1RAAMBO/03, and (ii) M2 RAAMBO/04 were developed.","PeriodicalId":164482,"journal":{"name":"EAS Journal of Biotechnology and Genetics","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116641329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}