Journal of natural toxins最新文献_第8页

Cobrotoxin: structure and function. 蛇毒素:结构与功能。

Journal of natural toxins Pub Date : 1999-06-01

C C Yang

{"title":"Cobrotoxin: structure and function.","authors":"C C Yang","doi":"","DOIUrl":"","url":null,"abstract":"Cobrotoxin is the main neurotoxic protein isolated from the venom of Taiwan cobra Naja naja atra. It is a small, basic protein consisting of a single polypeptide chain of 62 amino acids, cross-linked by four disulfide bonds. The disulfide bonds and Tyr-25 which is buried in the molecule form a central core to maintain and stabilize the active conformation of the toxin. Selective and stepwise chemical modifications of cobrotoxin indicate that at least two cationic groups, an epsilon-amino group of Lys-47 and a guanidino group of Arg-33, both of which are common to all known postsynaptic neurotoxins, held at a certain critical distance in the molecule, are functionally important for its neuromuscular blocking activity. The cDNA encoding cobrotoxin was constructed from the cellular RNA isolated from the venom glands of Naja naja atra by reverse transcription polymerase chain reaction. Sequencing several clones containing about 0.5 Kb DNA inserts contained a complete and full-length reading frame of 249 base pairs covering a precursor of cobrotoxin gene with a deduced mature protein sequence of 62 amino acids which are identical to the amino acid sequence of cobrotoxin and a 21 amino acid segment of signal peptide. Expression of cobrotoxin in E. coli vector generated a polypeptide which can cross-react with the antisera against the native cobrotoxin.","PeriodicalId":16437,"journal":{"name":"Journal of natural toxins","volume":"8 2","pages":"221-33"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21276841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein toxins produced by pathogenic vibrios. 由致病性弧菌产生的蛋白质毒素。

Journal of natural toxins Pub Date : 1999-06-01

S Shinoda

引用次数: 0

Acute goby poisoning in southern Taiwan. 台湾南部发生急性虾虎鱼中毒。

Journal of natural toxins Pub Date : 1999-02-01

S J Lin, J B Chen, K T Hsu, D F Hwang

引用次数: 0

History of USDA poisonous plant research. 美国农业部有毒植物研究历史。

Journal of natural toxins Pub Date : 1999-02-01

L F James

{"title":"History of USDA poisonous plant research.","authors":"L F James","doi":"","DOIUrl":"","url":null,"abstract":"Research on poisonous plants was instituted by the United States Department of Agriculture (USDA) as a result of serious livestock poisoning by plants as the pioneers moved west in the mid-to-late 1800s and early 1900s. Historical records indicate the USDA began poisonous plant research in 1894 under the direction of Mr. V. K. Chestnut, a botanist (Table 1 briefly summarizes those who have directed poisonous plant research from the inception to the present). Mr. Chestnut's responsibility (1894-1904) was primarily administrative, although he did extensive field work in Washington and Montana. Temporary field stations were set up to study specific poisonous plant problems. These included field stations at Hugo and Woodland Park, Colorado, and Imperial, Nebraska (1905-1909), to study locoweed; Gunnison, Colorado (1910-1912), to primarily study larkspur; and Greycliff, Montana (1912-1915), to study the poisonous plants of the Yellowstone Valley. Dr. Rodney True replaced Mr. Chestnut in 1904 and in 1905 hired Dr. C. D. Marsh (1905-1930) to establish the temporary field stations listed above. In 1915 a permanent facility was established at Salina, Utah, under the direction of C. D. Marsh who remained in charge until 1930 when he retired; he was followed by A. B. Clawson until 1937 when Dr. Ward Huffman was placed in charge. Research on poisonous plants was located at the Salina Experiment Station until 1955 when the station was closed and the laboratory moved to the campus of Utah State Agricultural College at Logan, Utah, where it is currently located. Dr. Wayne Binns was hired as the director of the laboratory in 1954 and retired in 1972. In 1972 Dr. Lynn F. James, who joined the PRPL staff in July 1957, was appointed as Research Leader and presently directs the research at the Poisonous Plant Research Laboratory.","PeriodicalId":16437,"journal":{"name":"Journal of natural toxins","volume":"8 1","pages":"3-26"},"PeriodicalIF":0.0,"publicationDate":"1999-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20964107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lupines, poison-hemlock and Nicotiana spp: toxicity and teratogenicity in livestock. 羽扇豆、毒芹和烟草属植物：对家畜的毒性和致畸性。

Journal of natural toxins Pub Date : 1999-02-01

K E Panter, L F James, D R Gardner

{"title":"Lupines, poison-hemlock and Nicotiana spp: toxicity and teratogenicity in livestock.","authors":"K E Panter, L F James, D R Gardner","doi":"","DOIUrl":"","url":null,"abstract":"Many species of lupines contain quinolizidine or piperidine alkaloids known to be toxic or teratogenic to livestock. Poison-hemlock (Conium maculatum) and Nicotiana spp. including N. tabacum and N. glauca contain toxic and teratogenic piperidine alkaloids. The toxic and teratogenic effects from these plant species have distinct similarities including maternal muscular weakness and ataxia and fetal contracture-type skeletal defects and cleft palate. It is believed that the mechanism of action of the piperidine and quinolizidine alkaloid-induced teratogenesis is the same; however, there are some differences in incidence, susceptible gestational periods, and severity between livestock species. Wildlife species have also been poisoned after eating poison-hemlock but no terata have been reported. The most widespread problem for livestock producers in recent times has been lupine-induced \"crooked calf disease.\" Crooked calf disease is characterized as skeletal contracture-type malformations and occasional cleft palate in calves after maternal ingestion of lupines containing the quinolizidine alkaloid anagyrine during gestation days 40-100. Similar malformations have been induced in cattle and goats with lupines containing the piperidine alkaloids ammodendrine, N-methyl ammodendrine, and N-acetyl hystrine and in cattle, sheep, goats, and pigs with poison-hemlock containing predominantly coniine or gamma-coniceine and N. glauca containing anabasine. Toxic and teratogenic effects have been linked to structural aspects of these alkaloids, and the mechanism of action is believed to be associated with an alkaloid-induced inhibition of fetal movement during specific gestational periods. This review presents a historical perspective, description and distribution of lupines, poison-hemlock and Nicotiana spp., toxic and teratogenic effects and management information to reduce losses.","PeriodicalId":16437,"journal":{"name":"Journal of natural toxins","volume":"8 1","pages":"117-34"},"PeriodicalIF":0.0,"publicationDate":"1999-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20963410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ponderosa pine and broom snakeweed: poisonous plants that affect livestock. 黄松和金雀花蛇草:影响牲畜的有毒植物。

Journal of natural toxins Pub Date : 1999-02-01

D R Gardner, L F James, K E Panter, J A Pfister, M H Ralphs, B L Stegelmeier

引用次数: 0

Locoweed grazing. 疯草放牧。

Journal of natural toxins Pub Date : 1999-02-01

M H Ralphs, L F James

{"title":"Locoweed grazing.","authors":"M H Ralphs, L F James","doi":"","DOIUrl":"","url":null,"abstract":"Locoweed is the most widespread poisonous plant problem in the western U. S. Eleven species of Astragalus and Oxytropis (and many varieties within these species) cause locoism. Many locoweed species are endemic and are restricted to a narrow niche or habitat. Other locoweed species experience extreme population cycles; the population explodes in wet years and dies off in drought. A few species, such as O. sericea, are relatively stable and cause persistent poisoning problems. Knowledge of where locoweeds grow and the environmental conditions when they become a threat is important to manage livestock and avoid poisoning. Locoweeds are relatively palatable. Many locoweeds are the first plants to begin growth in the spring and regrow in the fall. Livestock generally prefer the green-growing locoweeds to other forage that is dormant in the late fall, winter, and spring. The most effective management strategy is to deny livestock access to locoweeds during critical periods when they are more palatable than the associated forage. Herbicides can control existing locoweed populations and provide \"safe\" pastures for critical periods. However, locoweed seed in soil will germinate and re-establish when environmental condition are favorable. Good range management and wise grazing strategies can provide adequate forage for livestock and prevent them from grazing locoweed during non-critical periods of the year when it is relatively less palatable than associated forages.","PeriodicalId":16437,"journal":{"name":"Journal of natural toxins","volume":"8 1","pages":"47-51"},"PeriodicalIF":0.0,"publicationDate":"1999-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20963405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The pathogenesis and toxicokinetics of locoweed (Astragalus and Oxytropis spp.) poisoning in livestock. 家畜黄芪(Astragalus and Oxytropis spp.)中毒的发病机制和毒性动力学。

Journal of natural toxins Pub Date : 1999-02-01

B L Stegelmeier, L F James, K E Panter, M H Ralphs, D R Gardner, R J Molyneux, J A Pfister

{"title":"The pathogenesis and toxicokinetics of locoweed (Astragalus and Oxytropis spp.) poisoning in livestock.","authors":"B L Stegelmeier, L F James, K E Panter, M H Ralphs, D R Gardner, R J Molyneux, J A Pfister","doi":"","DOIUrl":"","url":null,"abstract":"Locoweed poisoning is a chronic disease that develops in livestock grazing for several weeks on certain Astragalus and Oxytropis spp. that contain the locoweed toxin, swainsonine. The purpose of this review is to present recent research on swainsonine toxicokinetics and locoweed-induced clinical and histologic lesions. Swainsonine inhibits cellular mannosidases resulting in lysosomal storage disease similar to genetic mannosidosis. Diagnosis of clinical poisoning is generally made by documenting exposure, identifying the neurologic signs, and analyzing serum for alpha-mannosidase activity and swainsonine. All tissues of poisoned animals contained swainsonine, and the clearance rates from most tissues was about 20 hours (T1/2 half life). The liver and kidney had longer rate of about 60 hours (T1/2). This suggests that poisoned animals should be allowed a 28-day withdrawal to insure complete swainsonine clearance. Poisoning results in vacuolation of most tissues that is most obvious in neurons and epithelial cells. Most of these histologic lesions resolved shortly after poisoning is discontinued; however, some neurologic changes are irreversible and permanent.","PeriodicalId":16437,"journal":{"name":"Journal of natural toxins","volume":"8 1","pages":"35-45"},"PeriodicalIF":0.0,"publicationDate":"1999-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20964109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Larkspur (Delphinium spp.) poisoning in livestock. 家畜的飞燕草中毒。

Journal of natural toxins Pub Date : 1999-02-01

J A Pfister, D R Gardner, K E Panter, G D Manners, M H Ralphs, B L Stegelmeier, T K Schoch

{"title":"Larkspur (Delphinium spp.) poisoning in livestock.","authors":"J A Pfister, D R Gardner, K E Panter, G D Manners, M H Ralphs, B L Stegelmeier, T K Schoch","doi":"","DOIUrl":"","url":null,"abstract":"Larkspurs (Delphinium spp.) are toxic plants that contain numerous diterpenoid alkaloids which occur as one of two structural types: (1) lycotonine, and (2) 7,8-methylenedioxylycoctonine (MDL-type). Among the lycoctonine type alkaloids are three N-(methylsuccinimido) anthranoyllycoctonine (MSAL-type) alkaloids which appear to be most toxic: methyllycaconitine (MLA), 14-deacetylnudicauline (DAN), and nudicauline. An ester function at C-18 is an important structural requirement for toxicity. Intoxication results from neuromuscular paralysis, as nicotinic acetylcholine receptors in the muscle and brain are blocked by toxic alkaloids. Clinical signs include labored breathing, rapid and irregular heartbeat, muscular weakness, and collapse. Toxic alkaloid concentration generally declines in tall larkspurs with maturation, but alkaloid concentration varies over years and from plant to plant, and is of little use for predicting consumption by cattle. Knowledge of toxic alkaloid concentration is valuable for management purposes when cattle begin to eat larkspur. Cattle generally begin consuming tall larkspur after flowering racemes are elongated, and consumption increases as larkspur matures. Weather is also a major factor in cattle consumption, as cattle tend to eat more larkspur during or just after summer storms. Management options that may be useful for livestock producers include conditioning cattle to avoid larkspur (food aversion learning), grazing tall larkspur ranges before flowering (early grazing) and after seed shatter (late grazing), grazing sheep before cattle, herbicidal control of larkspur plants, and drug therapy for intoxicated animals. Some potentially fruitful research avenues include examining alkaloid chemistry in low and plains larkspurs, developing immunologic methods for analyzing larkspur alkaloids, developing drug therapy, and devising grazing regimes specifically for low and plains larkspur.","PeriodicalId":16437,"journal":{"name":"Journal of natural toxins","volume":"8 1","pages":"81-94"},"PeriodicalIF":0.0,"publicationDate":"1999-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20963408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Locoweeds: effects on reproduction in livestock. 野草:对牲畜繁殖的影响。

Journal of natural toxins Pub Date : 1999-02-01

K E Panter, L F James, B L Stegelmeier, M H Ralphs, J A Pfister

{"title":"Locoweeds: effects on reproduction in livestock.","authors":"K E Panter, L F James, B L Stegelmeier, M H Ralphs, J A Pfister","doi":"","DOIUrl":"","url":null,"abstract":"Locoweeds (species of Oxytropis and Astragalus containing the toxin swainsonine) cause severe adverse effects on reproductive function in livestock. All aspects of reproduction can be affected: mating behavior and libido in males; estrus in females; abortion/embryonic loss of the fetus; and behavioral retardation of offspring. While much research has been done to describe and histologically characterize these effects, we have only begun to understand the magnitude of the problem, to define the mechanisms involved, or to develop strategies to prevent losses. Recent research has described the effects of locoweed ingestion in cycling cows and ewes. Briefly, feeding trials with locoweeds in cycling and pregnant cows have demonstrated ovarian dysfunction in a dose-dependent pattern, delayed estrus, extended estrous cycle length during the follicular and luteal phases, delayed conception (repeat breeders), and hydrops and abortion. Similar effects were observed in sheep. In rams, locoweed consumption altered breeding behavior, changed libido, and inhibited normal spermatogenesis. Neurological dysfunction also inhibited normal reproductive behavior, and some of these effects were permanent and progressive. In this article we briefly review the pathophysiological effects of locoweeds on reproduction.","PeriodicalId":16437,"journal":{"name":"Journal of natural toxins","volume":"8 1","pages":"53-62"},"PeriodicalIF":0.0,"publicationDate":"1999-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20963406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0