Journal of natural toxins最新文献_第2页

Improved purification and yield of the Egyptian snake Cerastes cerastes antitoxin by the use of caprylic acid. 用辛酸提高了埃及蛇蜡抗毒素的纯化和产率。

Journal of natural toxins Pub Date : 2002-12-01

Salwa S Seddik, George Abdel Malak, Madiha H Helmy

引用次数: 0

Occurrence of a food poisoning incident by palytoxin from a serranid Epinephelus sp. in Japan. 日本发生一宗由石斑鱼(serranid Epinephelus sp.)的孢毒素引起的食物中毒事件。

Journal of natural toxins Pub Date : 2002-12-01

Shigeto Taniyama, Yahia Mahmud, Masamitsu Terada, Tomohiro Takatani, Osamu Arakawa, Tamao Noguchi

引用次数: 0

Purification and characterization of alpha-mucrofibrase, a novel serine protease with alpha-fibrinogenase activity from the venom of Chinese Habu (Trimeresurus mucrosquamatus). 具有α -纤维蛋白原酶活性的新型丝氨酸蛋白酶α -多纤酶的纯化及特性研究。

Journal of natural toxins Pub Date : 2002-12-01

Qin Wei, Yang Jin, Qui-Min Lu, Ji-Fu Wei, Wang-Yu Wang, Yu-Liang Xiong

引用次数: 0

Extremely low nerve growth facior (NGF) activity of sea snake (Hydrophiidae) venoms. 海蛇(水螅科)毒液的神经生长因子活性极低。

Journal of natural toxins Pub Date : 2002-12-01

Khafizova Mariam, Anthony T Tu

引用次数: 0

Evidence for a neurotoxic activity in crude venom of the stonefish (Synanceia verrucosa). 石鱼粗毒液中神经毒性活性的证据。

Journal of natural toxins Pub Date : 2002-12-01

Patrick Breton, Iroudayanadin Delamanche, Janine Buée, Françoise Goudey-Perriére, Claude Perriére

{"title":"Evidence for a neurotoxic activity in crude venom of the stonefish (Synanceia verrucosa).","authors":"Patrick Breton, Iroudayanadin Delamanche, Janine Buée, Françoise Goudey-Perriére, Claude Perriére","doi":"","DOIUrl":"","url":null,"abstract":"The neurotoxic effects of the Synanceia verrucosa venom were investigated in rodents. After intracranial injection in mice (50-125 ng/g), venom induced constant symptoms such as ataxia, circling, partial or complete reversible limbs paralysis, scratching, rolling, sleep-like periods and violent clonic seizure conducing in few seconds to death. EEG alterations occurring in rat brain after intracerebroventricular injection (50-100 microg) were precised. An initial phase was characterized by short repetitive tonic seizure periods together with a significant rise of the relative power in the delta band, no significant modification of the theta II rhythm (4-7 Hz), a decreasing of energy in theta I (7-12 Hz) and 15-40 Hz bands. A second phase was characterized by a marked generalized slowing with transient drastic decreasing of the amplitude and flattening of cortical EEG (comatose state) as the main elements. Propanolol did not reverse the EEG effects of the venom except a slight decrease of the slow wave amplitude. Previous intracerebroventricular administration of a K+(ATP) blocker generally decrease the delay of death. Histopathologic examination of the brain of surviving animals did not reveal any microscopic lesions. These results suggest (1) a complex mechanism of the venom in its neuropathologic expression; (2) at the doses tested, symptoms are not related to adrenergic pathways, K+(ATP) channel opener (verrucotoxin) is not implied in the neurotoxic effect, and the effect of the venom, which not affecting the theta II rhythm, seemed not to be exerted through cholinergic pathway.","PeriodicalId":16437,"journal":{"name":"Journal of natural toxins","volume":"11 4","pages":"305-13"},"PeriodicalIF":0.0,"publicationDate":"2002-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22174851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A pilot experiment for production of Malayan krait antivenom: immunization of rabbits with Bungarus candidus venom. 马来响尾蛇抗蛇毒血清生产的中试研究:白蜡响尾蛇毒免疫家兔。

Journal of natural toxins Pub Date : 2002-12-01

Lawan Chanhome, Sununta Puempunpanich, Tamotsu Omori-Satoh, Narongsak Chaiyabutr, Visith Sitprija

{"title":"A pilot experiment for production of Malayan krait antivenom: immunization of rabbits with Bungarus candidus venom.","authors":"Lawan Chanhome, Sununta Puempunpanich, Tamotsu Omori-Satoh, Narongsak Chaiyabutr, Visith Sitprija","doi":"","DOIUrl":"","url":null,"abstract":"Immunization with Bungarus candidus venom was performed in four rabbits at high dose (initial dose, 75 microg/kg) and low dose (initial dose, 50 microg/kg). Each dose group consisted of two rabbits; one rabbit received the venom subcutaneously (s.c.) and the other intradermally (i.d.). The venom was injected as emulsified solutions with the same volume of Freund's complete adjuvant until the 4th immunization, thereafter as plain solutions. By stepwise increments of the immunizing dose, the higher dose group received a dose of 200 microg/kg and the lower dose group 150 microg/kg after the 5th immunization, respectively. Thereafter, seven additional immunizations were performed within six months. All rabbits were sacrificed two weeks after the last immunization (12th). Antilethal activity of the immunized antisera thus obtained was determined not only with the homologous venom but also with two heterologous venoms from Bungarus fasciatus and Bungarus flaviceps. Immunodiffusion analysis was also performed with these venoms. The results obtained in this pilot trial provided useful information for production of Malayan krait antivenom at Queen Saovabha Memorial Institute.","PeriodicalId":16437,"journal":{"name":"Journal of natural toxins","volume":"11 4","pages":"353-6"},"PeriodicalIF":0.0,"publicationDate":"2002-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22173588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical characterization of the soluble alkaline phosphatase isolated from the venomous snake W. aegyptia. 埃及伊蛇可溶性碱性磷酸酶的生化特性研究。

Journal of natural toxins Pub Date : 2002-12-01

Saad S M Al-Saleh

引用次数: 0

Bacterial toxins--an overview. 细菌毒素——概述。

Journal of natural toxins Pub Date : 2000-11-01

S S Lahiri

{"title":"Bacterial toxins--an overview.","authors":"S S Lahiri","doi":"","DOIUrl":"","url":null,"abstract":"Toxins are non-replicating agents of biological origin. They are non-infectious, non-contagious, and non-curable by antibiotics or chemotherapeutic agents. However, individuals can be protected by vaccination. The multifactorial nature of virulence of toxin and toxin producers, produces comparative and cooperative pathogenesis, and this makes studies all the more difficult. Antibody raised against all components helps in this pursuit. The toxins have been classified into seven different classes and over 44 bacterial toxins have been discussed. The botulinum toxin is by far the most toxic substance in the world. All the toxins produced are either secreted out, called exotoxins (proteins), or are entrapped in the cell membrane, called endotoxins (lipopolysaccharides). These toxins are di-chain molecules, internalized into the cell by receptor mediated endocytosis, and ADP-ribosylation is the most common mode of action. The toxins produced by bacteria are enterotoxins, neurotoxins, cytotoxins, lysins (e.g., hemolysin), gangrene producing toxins. However, a single bacteria often produces more than one toxin. Bacterial toxins, which are primarily harmful, are also being used for the cure of cancer, killing of mosquito larvae, understanding of basic sciences like ADP-ribosylation, etc.","PeriodicalId":16437,"journal":{"name":"Journal of natural toxins","volume":"9 4","pages":"381-408"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21949575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction of tetanus toxin derived hybrid proteins with neuronal cells. 破伤风毒素衍生的杂交蛋白与神经细胞的相互作用。

Journal of natural toxins Pub Date : 2000-11-01

D M Figueiredo, C C Matthews, D A Parks, N F Fairweather, G Dougan, S G Wilt, P S Fishman

{"title":"Interaction of tetanus toxin derived hybrid proteins with neuronal cells.","authors":"D M Figueiredo, C C Matthews, D A Parks, N F Fairweather, G Dougan, S G Wilt, P S Fishman","doi":"","DOIUrl":"","url":null,"abstract":"The non-toxic ganglioside binding domain of tetanus toxin (Hc fragment C or TTC) has been studied as a vector for delivering therapeutic proteins to neurons. There is little information on the cellular processing of proteins delivered by linkage to TTC. We have evaluated the cellular handling of a multi-domain hybrid protein containing TTC and both the human enzyme superoxide dismutase and the maltose binding protein from E. coli. Binding, internalization, and cleavage of this protein during prolonged incubation with fetal cortical neurons or cells of the N18-RE-105 line was evaluated by immunoblot analysis, ELISA, and immunocytochemistry. Hybrid proteins were bound and internalized in a manner very similar to TTC. Internalized proteins showed long-term stability within cells, and were degraded into predictable large protein fragments in both cell types. Fragments that were cleaved away from the TTC domain were released into extracellular fluid after internalization. Proteins coupled to TTC share its long-term stability after cellular internalization. After internalization, dissociation of proteins linked to TTC facilitates their release from the cell, but not into other cellular compartments such as the cytosol. TTC linked proteins are probably enclosed within a stable endosomal compartment throughout their cellular lifetime.","PeriodicalId":16437,"journal":{"name":"Journal of natural toxins","volume":"9 4","pages":"363-79"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21949574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An epidemic survey on freshwater puffer poisoning in Bangladesh. 孟加拉国淡水河豚中毒的流行病学调查。

Journal of natural toxins Pub Date : 2000-11-01

Y Mahmud, O Arakawa, T Noguchi

引用次数: 0