Journal of Mammary Gland Biology and Neoplasia最新文献_第8页

Correction to: Breast Cancer Response to Therapy: Can microRNAs Lead the Way? 乳腺癌对治疗的反应:microrna能引领潮流吗?

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2021-06-01 DOI: 10.1007/s10911-021-09480-9

Nina Petrović, Irina Nakashidze, Milica Nedeljković

引用次数: 3

A Method to Pre-Screen Rat Mammary Gland Whole-Mounts Prior To RNAscope. 一种RNAscope前大鼠乳腺全载预筛选方法。

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2021-06-01 Epub Date: 2021-04-17 DOI: 10.1007/s10911-021-09484-5

Emily L Duderstadt, Mary Ann Sanders, David J Samuelson

{"title":"A Method to Pre-Screen Rat Mammary Gland Whole-Mounts Prior To RNAscope.","authors":"Emily L Duderstadt, Mary Ann Sanders, David J Samuelson","doi":"10.1007/s10911-021-09484-5","DOIUrl":"https://doi.org/10.1007/s10911-021-09484-5","url":null,"abstract":"RNAscope is a quantitative in situ gene expression measurement technique that preserves the spatial aspect of intact tissue; thus, allowing for comparison of specific cell populations and morphologies. Reliable and accurate measurement of gene expression in tissue is dependent on preserving RNA integrity and the quantitative nature of RNAscope. The purpose of this study was to determine if the quantitative nature of RNAscope was retained following processing and carmine staining of mammary gland whole-mounts, which are commonly used to identify lesions, such as hyperplasia and ductal carcinoma in situ (DCIS). We were concerned that handling and procedures required to visualize microscopic disease lesions might compromise RNA integrity and the robustness of RNAscope. No effect on the quantitative abilities of RNAscope was detected when mammary gland whole-mounts were pre-screened for lesions of interest prior to RNAscope. This was determined in comparison to tissue that had been formalin-fixed and paraffin embedded (FFPE) immediately after collection. The ability to pre-screen whole-mounts allowed unpalpable diseased lesions to be identified without labor-intensive serial sectioning of tissue samples to find diseased tissue. This method is applicable to evaluate mammary gland whole-mounts during normal mammary gland development, function, and disease progression.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":"26 2","pages":"113-120"},"PeriodicalIF":2.5,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10911-021-09484-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38889058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Development and Pathology of the Equine Mammary Gland. 马乳腺的发育和病理学。

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2021-06-01 Epub Date: 2020-12-05 DOI: 10.1007/s10911-020-09471-2

Katherine Hughes

{"title":"Development and Pathology of the Equine Mammary Gland.","authors":"Katherine Hughes","doi":"10.1007/s10911-020-09471-2","DOIUrl":"10.1007/s10911-020-09471-2","url":null,"abstract":"An understanding of the anatomy, histology, and development of the equine mammary gland underpins study of the pathology of diseases including galactorrhoea, agalactia, mastitis, and mammary tumour development. This review examines the prenatal development of the equine mammary gland and the striking degree to which the tissue undergoes postnatal development associated with the reproductive cycle. The gland is characterised by epithelial structures arranged in terminal duct lobular units, similar to those of the human breast, supported by distinct zones of intra- and interlobular collagenous stroma. Mastitis and mammary carcinomas are two of the most frequently described equine mammary pathologies and have an overlap in associated clinical signs. Mastitis is most frequently associated with bacterial aetiologies, particularly Streptococcus spp., and knowledge of the process of post-lactational regression can be applied to preventative husbandry strategies. Equine mammary tumours are rare and carry a poor prognosis in many cases. Recent studies have used mammosphere assays to reveal novel insights into the identification and potential behaviour of mammary stem/progenitor cell populations. These suggest that mammospheres derived from equine cells have different growth dynamics compared to those from other species. In parallel with studying the equine mammary gland in order to advance knowledge of equine mammary disease at the interface of basic and clinical science, there is a need to better understand equine lactational biology. This is driven in part by the recognition of the potential value of horse and donkey milk for human consumption, particularly donkey milk in children with 'Cow Milk Protein Allergy'.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":"26 2","pages":"121-134"},"PeriodicalIF":2.5,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38338934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Breast Cancer Response to Therapy: Can microRNAs Lead the Way? 乳腺癌对治疗的反应:microrna能引领潮流吗?

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2021-06-01 Epub Date: 2021-01-21 DOI: 10.1007/s10911-021-09478-3

Nina Petrović, Irina Nakashidze, Milica Nedeljković

{"title":"Breast Cancer Response to Therapy: Can microRNAs Lead the Way?","authors":"Nina Petrović, Irina Nakashidze, Milica Nedeljković","doi":"10.1007/s10911-021-09478-3","DOIUrl":"https://doi.org/10.1007/s10911-021-09478-3","url":null,"abstract":"Breast cancer (BC) is a leading cause of death among women with malignant diseases. The selection of adequate therapies for highly invasive and metastatic BCs still represents a major challenge. Novel combinatorial therapeutic approaches are urgently required to enhance the efficiency of BC treatment. Recently, microRNAs (miRNAs) emerged as key regulators of the complex mechanisms that govern BC therapeutic resistance and susceptibility. In the present review we aim to critically examine how miRNAs influence BC response to therapies, or how to use miRNAs as a basis for new therapeutic approaches. We summarized recent findings in this rapidly evolving field, emphasizing the challenges still ahead for the successful implementation of miRNAs into BC treatment while providing insights for future BC management.The goal of this review was to propose miRNAs, that might simultaneously improve the efficacy of all four therapies that are the backbone of current BC management (radio-, chemo-, targeted, and hormone therapy). Among the described miRNAs, miR-21 and miR-16 emerged as the most promising, closely followed by miR-205, miR-451, miR-182, and miRNAs from the let-7 family. miR-21 inhibition might be the best choice for future improvement of invasive BC treatment.New therapeutic strategies of miRNA-based agents alongside current standard treatment modalities could greatly benefit BC patients. This review represents a guideline on how to navigate this elaborate puzzle.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":"26 2","pages":"157-178"},"PeriodicalIF":2.5,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10911-021-09478-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38768001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

A Comparative Review of the Extrinsic and Intrinsic Factors Regulating Lactose Synthesis. 调节乳糖合成的外因与内因的比较研究进展。

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2021-06-01 Epub Date: 2021-06-14 DOI: 10.1007/s10911-021-09491-6

Anna Sadovnikova, Sergio C Garcia, Russell C Hovey

引用次数: 5

Cancer-Associated Fibroblasts in the Breast Tumor Microenvironment. 乳腺肿瘤微环境中癌症相关成纤维细胞的研究

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2021-06-01 Epub Date: 2021-01-04 DOI: 10.1007/s10911-020-09475-y

María Belén Giorello, Francisco Raúl Borzone, Vivian Labovsky, Flavia Valeria Piccioni, Norma Alejandra Chasseing

引用次数: 17

Deep Learning Enables Individual Xenograft Cell Classification in Histological Images by Analysis of Contextual Features. 通过分析上下文特征，深度学习使组织学图像中的单个异种移植物细胞分类成为可能。

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2021-06-01 Epub Date: 2021-05-17 DOI: 10.1007/s10911-021-09485-4

Quentin Juppet, Fabio De Martino, Elodie Marcandalli, Martin Weigert, Olivier Burri, Michael Unser, Cathrin Brisken, Daniel Sage

{"title":"Deep Learning Enables Individual Xenograft Cell Classification in Histological Images by Analysis of Contextual Features.","authors":"Quentin Juppet, Fabio De Martino, Elodie Marcandalli, Martin Weigert, Olivier Burri, Michael Unser, Cathrin Brisken, Daniel Sage","doi":"10.1007/s10911-021-09485-4","DOIUrl":"https://doi.org/10.1007/s10911-021-09485-4","url":null,"abstract":"Patient-Derived Xenografts (PDXs) are the preclinical models which best recapitulate inter- and intra-patient complexity of human breast malignancies, and are also emerging as useful tools to study the normal breast epithelium. However, data analysis generated with such models is often confounded by the presence of host cells and can give rise to data misinterpretation. For instance, it is important to discriminate between xenografted and host cells in histological sections prior to performing immunostainings. We developed Single Cell Classifier (SCC), a data-driven deep learning-based computational tool that provides an innovative approach for automated cell species discrimination based on a multi-step process entailing nuclei segmentation and single cell classification. We show that human and murine cell contextual features, more than cell-intrinsic ones, can be exploited to discriminate between cell species in both normal and malignant tissues, yielding up to 96% classification accuracy. SCC will facilitate the interpretation of H&E- and DAPI-stained histological sections of xenografted human-in-mouse tissues and it is open to new in-house built models for further applications. SCC is released as an open-source plugin in ImageJ/Fiji available at the following link: https://github.com/Biomedical-Imaging-Group/SingleCellClassifier .","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":"26 2","pages":"101-112"},"PeriodicalIF":2.5,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10911-021-09485-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39002480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Cellular Organization of the Mammary Gland: Insights From Microscopy. 乳腺的细胞组织:从显微镜观察。

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2021-03-01 Epub Date: 2021-04-09 DOI: 10.1007/s10911-021-09483-6

Caleb A Dawson, Jane E Visvader

{"title":"The Cellular Organization of the Mammary Gland: Insights From Microscopy.","authors":"Caleb A Dawson, Jane E Visvader","doi":"10.1007/s10911-021-09483-6","DOIUrl":"https://doi.org/10.1007/s10911-021-09483-6","url":null,"abstract":"Despite rapid advances in our knowledge of the cellular heterogeneity and molecular regulation of the mammary gland, how these relate to 3D cellular organization remains unclear. In addition to hormonal regulation, mammary gland development and function is directed by para- and juxtacrine signaling among diverse cell-types, particularly the immune and mesenchymal populations. Precise mapping of the cellular landscape of the breast will help to decipher this complex coordination. Imaging of thin tissue sections has provided foundational information about cell positioning in the mammary gland and now technological advances in tissue clearing and subcellular-resolution 3D imaging are painting a more complete picture. In particular, confocal, light-sheet and multiphoton microscopy applied to intact tissue can fully capture cell morphology, position and interactions, and have the power to identify spatially rare events. This review will summarize our current understanding of mammary gland cellular organization as revealed by microscopy. We focus on the mouse mammary gland and cover a broad range of immune and stromal cell types at major developmental stages and give insights into important tissue niches and cellular interactions.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":"26 1","pages":"71-85"},"PeriodicalIF":2.5,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10911-021-09483-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25591321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Retraction Note to: Circ-TFCP2L1 Promotes the Proliferation and Migration of Triple Negative Breast Cancer through Sponging miR-7 by Inhibiting PAK1. 注:Circ-TFCP2L1通过抑制PAK1海绵化miR-7促进三阴性乳腺癌的增殖和迁移。

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2021-03-01 DOI: 10.1007/s10911-021-09481-8

Qian Wang, Zhouxiao Li, Yun Hu, Wubin Zheng, Weiwei Tang, Changyuan Zhai, Zhutong Gu, Jing Tao, Hanjin Wang

引用次数: 0

An Integrative Single-cell Transcriptomic Atlas of the Post-natal Mouse Mammary Gland Allows Discovery of New Developmental Trajectories in the Luminal Compartment. 出生后小鼠乳腺的综合单细胞转录组图谱允许发现新的腔室发育轨迹。

IF 2.5 4区医学

Journal of Mammary Gland Biology and Neoplasia Pub Date : 2021-03-01 Epub Date: 2021-04-28 DOI: 10.1007/s10911-021-09488-1

Martín E García Solá, Micaela Stedile, Inés Beckerman, Edith C Kordon

{"title":"An Integrative Single-cell Transcriptomic Atlas of the Post-natal Mouse Mammary Gland Allows Discovery of New Developmental Trajectories in the Luminal Compartment.","authors":"Martín E García Solá, Micaela Stedile, Inés Beckerman, Edith C Kordon","doi":"10.1007/s10911-021-09488-1","DOIUrl":"https://doi.org/10.1007/s10911-021-09488-1","url":null,"abstract":"The mammary gland is a highly dynamic organ which undergoes periods of expansion, differentiation and cell death in each reproductive cycle. Partly because of the dynamic nature of the gland, mammary epithelial cells (MECs) are extraordinarily heterogeneous. Single cell RNA-seq (scRNA-seq) analyses have contributed to understand the cellular and transcriptional heterogeneity of this complex tissue. Here, we integrate scRNA-seq data from three foundational reports that have explored the mammary gland cell populations throughout development at single-cell level using 10× Chromium Drop-Seq. We center our analysis on post-natal development of the mammary gland, from puberty to post-involution. The new integrated study corresponds to RNA sequences from 53,686 individual cells, which greatly outnumbers the three initial data sets. The large volume of information provides new insights, as a better resolution of the previously detected Procr+ stem-like cell subpopulation or the identification of a novel group of MECs expressing immune-like markers. Moreover, here we present new pseudo-temporal trajectories of MEC populations at two resolution levels, that is either considering all mammary cell subtypes or focusing specifically on the luminal lineages. Interestingly, the luminal-restricted analysis reveals distinct expression patterns of various genes that encode milk proteins, suggesting specific and non-redundant roles for each of them. In summary, our data show that the application of bioinformatic tools to integrate multiple scRNA-seq data-sets helps to describe and interpret the high level of plasticity involved in gene expression regulation throughout mammary gland post-natal development.","PeriodicalId":16413,"journal":{"name":"Journal of Mammary Gland Biology and Neoplasia","volume":"26 1","pages":"29-42"},"PeriodicalIF":2.5,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10911-021-09488-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38919586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5