{"title":"Evaluation of the Genotoxicity of Petroleum Refinery Effluents Using the Comet Assay in Oreochromis niloticus (Nile tilapia)","authors":"M. Hoshina, M. A. Marin-Morales","doi":"10.5132/JBSE.2010.01.012","DOIUrl":"https://doi.org/10.5132/JBSE.2010.01.012","url":null,"abstract":"In this study, the genotoxic effects of effluent and water samples from areas (Atibaia and Jaguari rivers) related to a petroleum refinery were assessed by comet assay carried out in erythrocytes of Oreochromis niloticus (Nile tilapia). It was registered, for the negative control test (pure ground water), the lowest levels of nucleus with damage, followed by the waters collected in the Jaguari river (environmental control). It was observed a higher level of nucleus with damage in the fishes exposed to the refinery effluents samples, which decrease with the increasing treatments levels. It was also observed, in relation to the negative control, a genotoxic effect in the Atibaia river sample collected after the effluents discharge. The results showed that, even after the treatments, the petroleum refinery effluents induced genotoxicity in the exposed fishes.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117170018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana Paula de Castro Rodrigues, Patrícia Oliveira Maciel, L. C. C. P. D. Silva, C. Albuquerque, A. F. Inácio, Mercedes Freire, A. R. Linde, N. Almosny, J. V. Andreata, Edison Dausacker Bidone, Zuleica Carmen Castilhos
{"title":"Biomarkers for Mercury Exposure in Tropical Estuarine Fish","authors":"Ana Paula de Castro Rodrigues, Patrícia Oliveira Maciel, L. C. C. P. D. Silva, C. Albuquerque, A. F. Inácio, Mercedes Freire, A. R. Linde, N. Almosny, J. V. Andreata, Edison Dausacker Bidone, Zuleica Carmen Castilhos","doi":"10.5132/JBSE.2010.01.003","DOIUrl":"https://doi.org/10.5132/JBSE.2010.01.003","url":null,"abstract":"Several studies have evaluated human risks due mercury (Hg) exposure through fish consumption. However, relatively few studies have explored effects of environmental Hg concentrations in biota, especially tropical fish species. The aim of this work was to assess in situ hematological, biochemical and genotoxic effects in tropical fish due to environmental exposure to mercury in estuarine ecosystems. A total of 282 fishes were collected from September 2003 to October 2005 in two estuarine areas: Ribeira Bay (reference area - 22° 55’ to 23° 02’ S and 44° 18’ to 44° 26’ W) and Guanabara Bay (highly impacted area by human activities - 22° 40’ to 23° 00’ S and 43° 00’ to 43° 20’ E). Total mercury levels in fish from Guanabara were twice higher than in Ribeira bay for the catfish species Genidens genidens (Ariidae), with significant differences among areas after standardization using length intervals (exposure time indicator). The species Haemulon steindachneri (Haemulidae) showed the highest mercury concentration, reflecting its position in trophic chain. Among effect biomarkers, only haematocrit, global leucometry and micronucleus assays seemed to reflect the differences on mercury exposure among areas, what may support their use for evaluations of fish exposure to mercury compounds. However, it’s necessary both laboratory experiments to establish cause-effect relationship and a continuous in situ study to obtain more information, involving more trophic levels, searching for sensible species to mercury exposure.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"121 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122076517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Desenvolvimento de um Esquema para Avaliação de Risco Ecológico em Ambientes Tropicais: Estudo de Caso da Contaminação por Metais em Santo Amaro da Purificação, Bahia, Brasil","authors":"Júlia Carina Niemeyer, E. D. Silva, Jma Sousa","doi":"10.5132/JBSE.2007.03.009","DOIUrl":"https://doi.org/10.5132/JBSE.2007.03.009","url":null,"abstract":"Development of a site specific ecological risk assessment scheme for tropical environments: a case study from Santo Amaro da Purificacao, Bahia, Brazil This project aims to further develop and adapt an ecological risk assessment scheme, following a tiered approach, for tropical systems. The study will be conducted in a metal contaminated area at Santo Amaro da Purificacao (Bahia, Brasil), resulting from the deposition of reminiscent metal waste of a lead smelter, inactive since 1993. The ecological risk will be evaluated joining information from three different lines of evidence: chemistry, ecology and ecotoxicology. The study includes the development and adaptation of terrestrial ecotoxicological bioassays for tropical environments that, together with bioassays with aquatic organisms, will allow an integrated assessment of both habitat and retention functions of the impacted soils. The final output of the project will be an environmental risk assessment scheme that will act as a base scheme for tropical environments with similar contamination profile.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115009748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Extraction and Concentration of Freshwater - and Seawater - Derived Dissolved Organic Matter for Use in Aquatic Toxicology Studies","authors":"S. Rodrigues, A. Bianchini","doi":"10.5132/JBSE.2007.03.011","DOIUrl":"https://doi.org/10.5132/JBSE.2007.03.011","url":null,"abstract":"Dissolved organic matter (DOM) is defined as the organic matter that passes through a 0.45 µm-mesh filter. Recent studies have shown the DOM importance in mitigating trace metals and organic pollutants toxicity. In general, studies with DOM are performed using commercial organic matter. However, it has been demonstrated that this organic matter has little structural similarity with the aquatic humic acids. Furthermore, a natural DOM is composed by different fractions, which can exhibit different complexing properties with metals. Thus, it is important to evaluate the effect of different sources of natural DOM on pollutants toxicity. To use natural DOM in aquatic toxicity tests, it is necessary to extract and provide suitable storage for samples in the laboratory. The ideal process for DOM isolation from natural waters should be capable of rapidly and effectively extracting large quantities of DOM from water without fractionation, chemical alteration and/or other losses. Therefore, in the present paper we describe the methodological approaches currently in use in our laboratory for extraction and concentration of both freshwater and seawater-derived DOM using reverse osmosis (freshwater) and solid phase extraction (seawater).","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"99 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117215804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Toxicidade de Resíduos Têxteis Tratados por Microrganismos","authors":"P. M. Dellamatrice, R. T. R. Monteiro","doi":"10.5132/JBSE.2006.01.013","DOIUrl":"https://doi.org/10.5132/JBSE.2006.01.013","url":null,"abstract":"Textile effluent toxicity after treatment by microorganisms The Municipal Treatment Station of Americana, Sao Paulo, Brazil, manages 400 L s ‐1 of effluent, from domestic and textile origin, which produces an average of 20 t of sludge per day. The degradation of the effluent and sludge by cyanobacteria, by fungus Pleurotus, and anaerobic system was evaluated after an activated sludge treatment. The detoxification was appraised with three bioassays comprising the cnidarian Hydra attenuata, the alga Selenastrum capricornutum and let-tuce seeds. All the microorganisms were able to degrade and detoxify the effluent. The strains of Pleurotus were able to decolorized the sludge, however the toxicity increased. The three strains produced high amounts of manganese-peroxi-dase and laccase in the presence of the effluent or sludge. The anaerobic/aerobic treatment was the most efficient for degradation of the effluent and reducing its toxicity.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125295007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. S. Barros, D. Angelis, L. T. Furlan, B. Corrêa-Junior
{"title":"Utilização de Planárias da Espécie Dugesia (Girardia) tigrina em Testes de Toxicidade de Efluente de Refinaria de Petróleo","authors":"G. S. Barros, D. Angelis, L. T. Furlan, B. Corrêa-Junior","doi":"10.5132/JBSE.2006.01.014","DOIUrl":"https://doi.org/10.5132/JBSE.2006.01.014","url":null,"abstract":"Use of freshwater planarians Dugesia (Girardia) tigrina testing toxicity of a petroleum refinery wastewater The action of wastewater from Paulinia Petroleum Refinery (REPLAN) was tested in freshwater planarian regeneration, from the species Dugesia (Girardia) tigrina. This chronic toxicity test was realized to establish conditions to use them as a monitoring species of the refinery treatment efficiency. The tested samples wore collected at six points: rio Jaguari (REPLAN water intake); wastewater beginning biological treatment (after physical treatment); wastewater entering sta-bilization lagoon (last stage of treatment); discharge of stabilization lagoon; upstream from effluent discharge at rio Atibaia; downstream from the effluent discharge. Drinking water was used as control. Five or eight adult planarian at regenera-tion stage was tested in each point. The adult tested specimens were selected and weren’t feed during the experiment. The test was initiated immediately after the removal of planarian’s head (cut with blade) and finalized when the regeneration was complete. It was detected high toxic effect only at the sample from the primary treatment discharge, before realiz-ing the aeration and biological treatment. We can conclude that REPLAN effluent, at the end of the treatment process don’t cause any effect in Dugesia (Girardia) tigrina cephalic regeneration.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"241 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115219416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. G. Seixas, Helena A. Kehrig, I. Moreira, Olaf Malm
{"title":"Selenio em Tecidos de Organismos Marinhos da Baa de Guanabara Brasil","authors":"T. G. Seixas, Helena A. Kehrig, I. Moreira, Olaf Malm","doi":"10.5132/JBSE.2006.01.005","DOIUrl":"https://doi.org/10.5132/JBSE.2006.01.005","url":null,"abstract":"","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117084257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. L. S. Sisinno, A. Rizzo, M. Bulus, D. A. Rocha, A. U. Soriano, J. Moreira, Manoel de Carvalho
{"title":"Application of Ecotoxicological Tests in a Preliminary Evaluation of Soils Treated on Bioreactor","authors":"C. L. S. Sisinno, A. Rizzo, M. Bulus, D. A. Rocha, A. U. Soriano, J. Moreira, Manoel de Carvalho","doi":"10.5132/JBSE.2007.02.008","DOIUrl":"https://doi.org/10.5132/JBSE.2007.02.008","url":null,"abstract":"Bioremediation processes are being applied nowadays for treatment of contaminated soils by petroleum products. However, only chemical analyses are used for efficiency verification, without taking into account a biological indicator. The aim of this work was the application of two ecotoxicological tests for the evaluation of a soil contaminated by crude oil, after treatment on a bioreactor, in different conditions. Sample A was treated with biostimulation (pH, nutrients and moisture control), while samples B and C, besides biostimulation was applied bulking material (sawdust; 10% w/w) also. The ecotoxicological tests chosen were luminescent bacteria Vibrio fischeri (ISO 11348-3:1998) and an avoidance behavior test with Eisenia fetida earthworms (still in a draft form). This test consists in the exposition of the organisms to contaminated soil and control soil samples, simultaneously, to evaluate the percentage of organisms that goes from the center of the recipient to the control sample sector. The results of avoidance behavior tests of samples A, B and C were, respectively, 52%, 29% and 50%. Those results indicate no toxicity, once the criteria for a sample be considered toxic is that more than 80% of the total exposed organisms are in the control soil at the end of the test. However, the test with Vibrio fischeri indicated toxicity for sample B (CE 50 13.8%), probably because of the recent contamination. Those results indicate that, although the importance of ecotoxicological tests inclusion for the complementation of bioremediation processes evaluation, at least two tests, using different organisms must be performed for a better consideration.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125633756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. E. Moron, V. L. P. Polez, R. Artoni, J. Ribas, H. Takahashi
{"title":"Estudo de Alterações na Concentração dos Íons Plasmáticos e da Indução de Micronúcleos em Piaractus mesopotamicus Exposto ao Herbicida Atrazina","authors":"S. E. Moron, V. L. P. Polez, R. Artoni, J. Ribas, H. Takahashi","doi":"10.5132/JBSE.2006.01.006","DOIUrl":"https://doi.org/10.5132/JBSE.2006.01.006","url":null,"abstract":"Study of alterations in the concentration of ions in the plasma and of the induction of micronucleus in Piaractus mesopotamicus exposed to the herbicide atrazine The pollution of the aquatic ecosystem has been increasing in the last years, mainly due to the industrial and agricultural development. The Atrazine pesticide is an important agent pollutant and it can harm the development and reproduction of several species of fish due to its toxicity. Piaractus mesopotamicus (pacu) is one of the species thoroughly comercialized and used in fish farms. The present study analyzed possible modifications in the concentration of ions in the plasma (Na + , Cl ‐ , K + , Ca 2+ ), in the blood pH and in the micronucleus frequency in pacu erythrocytes (N = 10 for group; Wt: 160.0 ± 5 g) exposed to the Atrazine herbicide (0.1 mg/L) during 96 hours in semi-static system. Samples of blood were collected, with aid of syringes heparinized, being centrifuged later and/or made smears for subsequent analysis of the micronucleus. The obtained plasma was used for the determinations of ions in the plasma. Cytological analyses showed significant differences (p < 0.0001) in the micronucleus frequency among the control group which was exposed to the atrazine herbicide. The blood pH stayed constant, but the ions concentration in the plasma Na + and Cl ‐ was, in relation to the control, sig-nificantly reduced (p < 0.05). The tested concentration of the herbicide probably provoked disturbances in the acid-base ionic balance and it can also be considered a clastogenic and poisonous product.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131514719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Fracácio, A. F. Campagna, B. Rodrigues, N. F. Verani, E. Espíndola
{"title":"Avaliação da Toxicidade do Endosulfan Sulfato sobre Juvenis de Danio Rerio (Cypriniformes, Cyprinidae)","authors":"R. Fracácio, A. F. Campagna, B. Rodrigues, N. F. Verani, E. Espíndola","doi":"10.5132/JBSE.2008.01.003","DOIUrl":"https://doi.org/10.5132/JBSE.2008.01.003","url":null,"abstract":"Evaluation of toxicity endosulfan sulfate on Danio rerio juvenilis (Cypriniformes, Cyprinidae) The objective of this work was to evaluate the toxicity of endosulfan sulfate toxicity (the most persistent form) in Danio rerio juveniles, considering the mortalities, in exposition of 96 hours, and survive and microscopic gill morphology in toxicity test partial chronic (7 days) in concentrations of 0.003 µg L ‐1 , 0.3 µg L ‐1 and 3.0 µg L ‐1 , near the levels detected in the upper Jacare-Guacu river basin (Sao Paulo, Brazil). The result of the mortality was detected CL50, 96 h of the 7.29 µg L ‐1 . Considering the survival, only the higher concentration (3 µg L ‐1 ) promoted toxicity to the test organisms. However, the histological analyses revealed gill changes such as hyperplasia, fusion between some secondary lamellae and thickening of the apex of some secondary lamellae. These alterations compromised the gases exchange and consequently the vital functions of the biological system. The obtained alterations were of first and second level, but these changes can either to return to normal conditions when chemistry conditions improve, or to progress to a third stage degree, which are irreversible, provoking the death of the individual. This results prove the importance of the histology as a tool to evaluate toxicity of chemical products, giving evidences that endosulfan sulfate is toxic to this fish specie at concentrations found in aquatic environments.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131408581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}