Journal of Integrative Agriculture最新文献_第8页

Physiological and transcriptome analyses of Chinese cabbage in response to drought stress 大白菜对干旱胁迫的生理响应和转录组分析

IF 4.8 1区农林科学

Journal of Integrative Agriculture Pub Date : 2024-03-22 DOI: 10.1016/j.jia.2024.03.067

Lin Chen, Chao Li, Jiahao Zhang, Zongrui Li, Qi Zeng, Qingguo Sun, Xiaowu Wang, Limin Zhao, Lugang Zhang, Baohua Li

引用次数: 0

Geographical origin identification of winter jujube (Ziziphus jujuba ‘Dongzao’) by using multi-element fingerprinting with chemometrics 利用多元素指纹图谱和化学计量学鉴定冬枣的地理产地

IF 4.8 1区农林科学

Journal of Integrative Agriculture Pub Date : 2024-03-22 DOI: 10.1016/j.jia.2024.03.065

Xiabing Kong, Qiusheng Chen, Min Xu, Yihui Liu, Xiaoming Li, Lingxi Han, Qiang Zhang, Haoliang Wan, Lu Liu, Xubo Zhao, Jiyun Nie

{"title":"Geographical origin identification of winter jujube (Ziziphus jujuba ‘Dongzao’) by using multi-element fingerprinting with chemometrics","authors":"Xiabing Kong, Qiusheng Chen, Min Xu, Yihui Liu, Xiaoming Li, Lingxi Han, Qiang Zhang, Haoliang Wan, Lu Liu, Xubo Zhao, Jiyun Nie","doi":"10.1016/j.jia.2024.03.065","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.065","url":null,"abstract":"Winter jujube ( ‘Dongzao’) is greatly appreciated by consumers for its excellent quality, but brand infringement frequently occurs in the market. Here, we first determined a total of 38 elements in 167 winter jujube samples from the main winter jujube producing areas of China by inductively coupled plasma mass spectrometer (ICP-MS). As a result, 16 elements (Mg, K, Mn, Cu, Zn, Mo, Ba, Be, As, Se, Cd, Sb, Ce, Er, Tl, and Pb) exhibited significant differences in samples from different producing areas. Supervised linear discriminant analysis (LDA) and orthogonal projection to latent structures discriminant analysis (OPLS-DA) showed better performance in identifying the origin of samples than unsupervised principal component analysis (PCA). LDA and OPLS-DA had a mean identification accuracy of 87.84 and 94.64% in the testing set, respectively. By using the multilayer perceptron (MLP) and C5.0, the prediction accuracy of the models could reach 96.36 and 91.06%, respectively. Based on the above four chemometric methods, Cd, Tl, Mo and Se were selected as the main variables and principal markers for the origin identification of winter jujube. Overall, this study demonstrates that it is practical and precise to identify the origin of winter jujube through multi-element fingerprint analysis with chemometrics, and may also provide reference for establishing the origin traceability system of other fruits.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140830044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disruption of a microvitellogenin gene impairs eggshell formation in Mythimna separata 微绒毛原蛋白基因的中断会影响分离贻贝的蛋壳形成

IF 4.8 1区农林科学

Journal of Integrative Agriculture Pub Date : 2024-03-18 DOI: 10.1016/j.jia.2024.03.057

Lingling Li, Junhong Fu, Changgeng Dai, Yuhang Zhou, Yang Hu, Hongbo Li

{"title":"Disruption of a microvitellogenin gene impairs eggshell formation in Mythimna separata","authors":"Lingling Li, Junhong Fu, Changgeng Dai, Yuhang Zhou, Yang Hu, Hongbo Li","doi":"10.1016/j.jia.2024.03.057","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.057","url":null,"abstract":"Microvitellogenin (mVg) belongs to the lepidopteran-specific lipoprotein_11 super family and is thought to be involved in insect reproduction. However, the function of mVg in reproduction has not been established. In this study, we identified and characterized in , a destructive insect pest of grain crops. is comprised of a 1,310 bp ORF encoding 437 amino acids. Structural analysis showed that MsmVg contains three conserved domains in the lipoprotein_11 super family, including the all-α-N-terminal domain (NTD), a homologous domain (PPD), and an all-β-C-terminal domain (CTD). was highly expressed in mature female adults and enriched in the ovaries of three-day-old female adults. Disruption of by RNA interference did not change the expression of , vitellogenin () or the vitellogenin receptor () in fat bodies, but it inhibited their transcription in ovaries. Phenotype analysis showed that knockdown of did not affect yolk deposition in ovaries, but impaired eggshell formation and led to a reduction in the reproductive capacity. Furthermore, knockdown of significantly inhibited the expression of genes associated with eggshell formation ( and ). Taken together, these data suggest that plays an important role in female reproduction by affecting eggshell formation in . , which provides a potential target for managing this insect pest.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140200498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein disulfide isomerase MoPdi1 regulates fungal development, virulence, and endoplasmic reticulum homeostasis in Magnaporthe oryzae1 蛋白二硫异构酶 MoPdi1 调控木格鲁氏真菌的发育、毒力和内质网稳态1

IF 4.8 1区农林科学

Journal of Integrative Agriculture Pub Date : 2024-03-16 DOI: 10.1016/j.jia.2024.03.054

Yu Wang, Xiaoru Kang, Xinyue Cui, Jinmei Hu, Yuemin Pan, Yizhen Deng, Shulin Zhang

{"title":"Protein disulfide isomerase MoPdi1 regulates fungal development, virulence, and endoplasmic reticulum homeostasis in Magnaporthe oryzae1","authors":"Yu Wang, Xiaoru Kang, Xinyue Cui, Jinmei Hu, Yuemin Pan, Yizhen Deng, Shulin Zhang","doi":"10.1016/j.jia.2024.03.054","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.054","url":null,"abstract":"Rice blast, caused by ,is a fungal disease that causes devastating damage to rice production worldwide. During infection, pathogens secrete effector proteins that modulate plant immunity. Disulfide bond formation catalyzed by protein disulfide isomerases (PDI) is essential for protein folding and maturation. However, the biological function of Pdi1 in has not yet been characterized. In this study, we identified the endoplasmic reticulum (ER)-located protein, MoPdi1, in . MoPdi1 regulates conidiation, cell wall stress, and pathogenicity of . Furthermore, the CGHC active sites in the a and a’ redox domain of MoPdi1 were essential for the biological function of . Further tests demonstrated that MoPdi1 was involved in the regulation of ER stress and positively regulated ER phagy. We also found that MoPdi1 interacted with MoHut1. Deletion of led to the bereft of MoHut1 dimerization, which depends on the formation of disulfide bonds. In addition, MoPdi1 affected the normal secretion of the cytoplasmic effector AVR-Pia. We provided evidence that MoHut1 is important for the vegetative growth, conidiation, and pathogenicity in . Therefore, our findings could provide a suitable target point for designing antifungal agrochemicals against rice blast fungus.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140200583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eureka lemon zinc finger protein ClDOF3.4 interacts with citrus yellow vein clearing virus coat protein to inhibit viral infection1 尤里卡柠檬锌指蛋白 ClDOF3.4 与柑橘黄筋清病毒衣壳蛋白相互作用，抑制病毒感染1

IF 4.8 1区农林科学

Journal of Integrative Agriculture Pub Date : 2024-03-16 DOI: 10.1016/j.jia.2024.03.049

Ping Liao, Ting Zeng, Mengyang Huangfu, Cairong Zheng, Jiequn Ren, Changyong Zhou, Yan Zhou

{"title":"Eureka lemon zinc finger protein ClDOF3.4 interacts with citrus yellow vein clearing virus coat protein to inhibit viral infection1","authors":"Ping Liao, Ting Zeng, Mengyang Huangfu, Cairong Zheng, Jiequn Ren, Changyong Zhou, Yan Zhou","doi":"10.1016/j.jia.2024.03.049","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.049","url":null,"abstract":"Citrus yellow vein clearing virus (CYVCV) is a new citrus virus that has become an important factor restricting the development of China'scitrus industry, and the CYVCV coat protein (CP) is associated with viral pathogenicity. In this study, the Eureka lemon zinc finger protein (ZFP) ClDOF3.4 was shown to interact with CYVCV CP and . Transient expression of in Eureka lemon induced the expression of salicylic acid (SA)-related and hypersensitive response marker genes, and triggered a reactive oxygen species burst, ion leakage necrosis, and the accumulation of free SA. Furthermore, the CYVCV titer in transgenic Eureka lemon plants was approximately 69.4% that in control plants 6 months after inoculation, withonly mild leaf chlorotic spots observed in those transgenic plants. Taken together, the results indicate that ClDOF3.4 not only interacts with CP but also induces an immune response in Eureka lemon by inducing the SA pathways. This is the first report that ZFP is involved in the immune response of a citrus viral disease, which provides a basis for further study of the molecular mechanism of CYVCV infection.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140200591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rapid and visual detection method for Crimean-Congo hemorrhagic fever virus by targeting S gene1 以 S 基因1 为靶标的克里米亚-刚果出血热病毒快速可视检测方法

IF 4.8 1区农林科学

Journal of Integrative Agriculture Pub Date : 2024-03-16 DOI: 10.1016/j.jia.2024.03.050

Xingqi Liu, Zengguo Cao, Boyi Li, Pei Huang, Yujie Bai, Jingbo Huang, Zanheng Huang, Yuanyuan Zhang, Yuanyuan Li, Haili Zhang, Hualei Wang

引用次数: 0

Genetic and biological properties of H9N2 avian influenza viruses isolated in central China from 2020 to 2022 2020 至 2022 年中国中部地区分离到的 H9N2 禽流感病毒的遗传和生物学特性

IF 4.8 1区农林科学

Journal of Integrative Agriculture Pub Date : 2024-03-16 DOI: 10.1016/j.jia.2024.03.055

Libin Liang, Yaning Bai, Wenyan Huang, Pengfei Ren, Xing Li, Dou Wang, Yuhan Yang, Zhen Gao, Jiao Tang, Xingchen Wu, Shimin Gao, Yanna Guo, Mingming Hu, Zhiwei Wang, Zhongbing Wang, Haili Ma, Junping Li

{"title":"Genetic and biological properties of H9N2 avian influenza viruses isolated in central China from 2020 to 2022","authors":"Libin Liang, Yaning Bai, Wenyan Huang, Pengfei Ren, Xing Li, Dou Wang, Yuhan Yang, Zhen Gao, Jiao Tang, Xingchen Wu, Shimin Gao, Yanna Guo, Mingming Hu, Zhiwei Wang, Zhongbing Wang, Haili Ma, Junping Li","doi":"10.1016/j.jia.2024.03.055","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.055","url":null,"abstract":"The H9N2 subtype of avian influenza virus (AIV) is widely prevalent in poultry and wild birds globally, and has become the predominant subtype circulating in poultry in China. The H9N2 AIV can directly or indirectly (by serving as a “donor virus”) infect humans, posing a significant threat to public health. Currently, there is a lack of in-depth research on the prevalence of H9N2 viruses in Shanxi Province, central China. In this study, we isolated 14 H9N2 AIVs from October 2020 to April 2022 in Shanxi Province, and genetic analysis revealed that these viruses belonged to seven different genotypes. Our study on animals revealed that the H9N2 strains we identified displayed high transmission efficiency among chicken populations, and exhibited diverse replication abilities within these birds. These viruses could replicate efficiently in the lungs of mice, with one strain also demonstrating the capacity to reproduce in organs like the brain and kidneys. At the cellular level, the replication ability of different H9N2 strains was evaluated using plaque formation assays and multi-step growth curve assays, revealing significant differences in the replication and proliferation efficiency of the various H9N2 viruses at the cellular level. The antigenicity analysis suggested that these isolates could be classified into twoseparate antigenic clusters. Our research provides crucial data to help understand the prevalence and biological characteristics of H9N2 AIVs in central China. It also highlights the necessity of enhancing the surveillance of H9N2 AIVs.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140200513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunogenicity and efficacy of an LNP-mRNA prepared from African Swine Fever Virus K205R1 非洲猪瘟病毒 K205R1 制备的 LNP-mRNA 的免疫原性和功效

IF 4.8 1区农林科学

Journal of Integrative Agriculture Pub Date : 2024-03-16 DOI: 10.1016/j.jia.2024.03.053

Chuanwen Tian, Yingnan Liu, Dongdong Di, Zhenhua Xie, Yao Li, Rongrong Wang, Jie Li, Jingyi Liu, Hongjun Chen

引用次数: 0

A bacterial protein Rhp-PSP inhibits plant viral proliferation through endoribonuclease activity1 细菌蛋白 Rhp-PSP 通过内切核酸酶活性抑制植物病毒增殖1

IF 4.8 1区农林科学

Journal of Integrative Agriculture Pub Date : 2024-03-16 DOI: 10.1016/j.jia.2024.03.051

Lijie Chen, Qianze Peng, Xiaohua Du, Weixing Zhang, Ju'e Cheng, Shu'e Sun, Deyong Zhang, Pin Su, Yong Liu

{"title":"A bacterial protein Rhp-PSP inhibits plant viral proliferation through endoribonuclease activity1","authors":"Lijie Chen, Qianze Peng, Xiaohua Du, Weixing Zhang, Ju'e Cheng, Shu'e Sun, Deyong Zhang, Pin Su, Yong Liu","doi":"10.1016/j.jia.2024.03.051","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.051","url":null,"abstract":"Plant virus causes massive crop losses globally. However, there is currently no effective measure to control plant viral disease. Previously, we identify an antiviral protein Rhp-PSP, produced by the bacterial strain JSC-3b. In this study, we discover that the antiviral activity of Rhp-PSP relies on its endoribonuclease activity. Convert the arginine (R) residue at position 129 onto alanine (A) abolish its endoribonuclease activity on coat protein (CP) RNA of tobacco mosaic virus (TMV), consequentially, compromised the antiviral activity of Rhp-PSP. Further investigation demonstrates that, the mutant Rhp-PSP is unable to form the homotrimer as the wild type, indicating the importance of quaternary junction for the endoribonuclease activity. Overexpression of Rhp-PSP in significantly enhances the resistance against TMV of seedlings, while expression of Rhp-PSP did not, confirming that endoribonuclease activity is responsible for the antiviral activity of Rhp-PSP. In addition, foliar spray of Rhp-PSP solution on tomato and pepper plants significantly reduces the disease index of viral diseases, indicating that Rhp-PSP shows potential to develop antiviral agent in practice.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140200494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel characterization of NADC30-like and NADC34-like PRRSV strains in China: epidemiological status and pathogenicity analysis of L1A variants1 中国NADC30-like和NADC34-like PRRSV毒株的新特征：L1A变体的流行病学现状和致病性分析1

IF 4.8 1区农林科学

Journal of Integrative Agriculture Pub Date : 2024-03-16 DOI: 10.1016/j.jia.2024.03.056

Hu Xu, Siyu Zhang, Zhenyang Guo, Chao Li, Bangjun Gong, Jinhao Li, Qi Sun, Jing Zhao, Menglin Zhang, Lirun Xiang, Yan-dong Tang, Chaoliang Leng, Jianan Wu, Qian Wang, Jinmei Peng, Guohui Zhou, Huairan Liu, Tongqing An, Xuehui Cai, Zhi-Jun Tian, Hongliang Zhang

{"title":"Novel characterization of NADC30-like and NADC34-like PRRSV strains in China: epidemiological status and pathogenicity analysis of L1A variants1","authors":"Hu Xu, Siyu Zhang, Zhenyang Guo, Chao Li, Bangjun Gong, Jinhao Li, Qi Sun, Jing Zhao, Menglin Zhang, Lirun Xiang, Yan-dong Tang, Chaoliang Leng, Jianan Wu, Qian Wang, Jinmei Peng, Guohui Zhou, Huairan Liu, Tongqing An, Xuehui Cai, Zhi-Jun Tian, Hongliang Zhang","doi":"10.1016/j.jia.2024.03.056","DOIUrl":"https://doi.org/10.1016/j.jia.2024.03.056","url":null,"abstract":"NADC34-like Porcine reproductive and respiratory syndrome virus (PRRSV), which first appeared in China in 2017, is currently one of the main epidemic strains in China. In this study, we found that a new variant of NADC34-like PRRSV evolved, named the L1A variant. The phylogenetics, epidemic status, and pathogenicity of the LA variants were subsequently comprehensively evaluated. Based on the results of the ORF5 phylogenetic analysis, the L1A variants were classified as NADC34-like PPRSV. All the strains had the same discontinuous 131-aa deletion in the NSP2 region (similar to that in the NADC30). Recombination analysis revealed that the L1A variants were recombinant viruses that contained an NADC30-like PRRSV skeleton, a nonstructural protein-encoding gene region obtained in part from JXA1-like PRRSV and a ORF2-ORF6 gene region partly obtained from NADC34-like PRRSV and that exhibited similar recombination patterns. We successfully isolated the L1A variant TZJ2756 from PAMs and Marc-145 cells. In animal experiments, TZJ2756 exhibited moderate pathogenicity in piglets, causing obvious clinical symptoms, namely, persistent fever, significantlyreduced body weight, interstitial edema and severe interstitial pneumonia in the lungs, and prolonged high-load viremia. L1A variants have been detected in at least 12 provinces in China and share many similar epidemiological characteristics with the American L1C variant. This research will enhance our understanding of the prevalence of L1A variants and furnish valuable data for the ongoing monitoring of NADC34-like PRRSV in China.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140200502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0