Journal of Mathematical and Fundamental Sciences最新文献_第10页

Effects of Conditioned Medium of Co-Culture IL-2 Induced NK Cells and Human Wharton’s Jelly Mesenchymal Stem Cells (hWJMSCs) on Apoptotic Gene Expression in a Breast Cancer Cell Line (MCF-7) IL-2诱导NK细胞与人华顿水母间充质干细胞(hWJMSCs)共培养条件培养基对乳腺癌细胞系MCF-7凋亡基因表达的影响

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Journal of Mathematical and Fundamental Sciences Pub Date : 2019-12-31 DOI: 10.5614/j.math.fund.sci.2019.51.3.1

W. Widowati, D. Jasaputra, Philips Onggowidjaja, S. Sumitro, M. A. Widodo, Ervi Afifah, Dwi Davidson Rihibiha, R. Rizal, A. Amalia, H. Kusuma, Harry Murti, I. Bachtiar

{"title":"Effects of Conditioned Medium of Co-Culture IL-2 Induced NK Cells and Human Wharton’s Jelly Mesenchymal Stem Cells (hWJMSCs) on Apoptotic Gene Expression in a Breast Cancer Cell Line (MCF-7)","authors":"W. Widowati, D. Jasaputra, Philips Onggowidjaja, S. Sumitro, M. A. Widodo, Ervi Afifah, Dwi Davidson Rihibiha, R. Rizal, A. Amalia, H. Kusuma, Harry Murti, I. Bachtiar","doi":"10.5614/j.math.fund.sci.2019.51.3.1","DOIUrl":"https://doi.org/10.5614/j.math.fund.sci.2019.51.3.1","url":null,"abstract":"Breast cancer (BC) is the most prevalent type of cancer among women and one of the major causes of cancer mortality in women. Metastasis in breast cancer (BC) occurs due to immunosurveillance deficiency, including impairment of natural killer (NK) cell maturation. Conditioned medium (CM) from human Wharton’s jelly mesenchymal stem cells (hWJMSC-CM) is known to possess anticancer activity. The CM of co-culture of human recombinant IL-2 treated NK cells and hWJMSCs is expected to boost anticancer activity toward BC cells which can be analyzed from the effect of CM towards secretion of effector molecules and expression of BC cell apoptosis-related genes, and cytotoxic granules in human recombinant IL-2 treated NK (IL-2 NK) and hWJMSCs (IL-2 hWJMSCs). TNF-α, IFN-γ, perforin, granzyme were measured by ELISA, while the inhibition of cell proliferation was measured by MTS assay and BC cell apoptosis by flow cytometry and apoptotic gene expression by RTPCR. CM from co-cultured hWJMSCs and IL-2 NK cells inhibited NK and BC cell proliferation, increased expression of Bax and p53 and decreased the number of Bcl-2 in BC cells. In conclusion, CM of co-culture IL-2 treated NK cells and hWJMSCs induce apoptosis in BC cells as indicated by increased Bax and p53 expression and decreased Bcl-2 expression.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77368736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Growth of Human Dermal Fibroblasts on Polyvinyl Alcohol-Silk Fibroin Nanofiber Scaffold 聚乙烯醇-丝素纳米纤维支架上人真皮成纤维细胞的生长

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Journal of Mathematical and Fundamental Sciences Pub Date : 2019-12-31 DOI: 10.5614/j.math.fund.sci.2019.51.3.7

Regina Giovanni, U. A. Wibowo, H. Judawisastra, A. Barlian

{"title":"Growth of Human Dermal Fibroblasts on Polyvinyl Alcohol-Silk Fibroin Nanofiber Scaffold","authors":"Regina Giovanni, U. A. Wibowo, H. Judawisastra, A. Barlian","doi":"10.5614/j.math.fund.sci.2019.51.3.7","DOIUrl":"https://doi.org/10.5614/j.math.fund.sci.2019.51.3.7","url":null,"abstract":"Skin tissue engineering is a developing technology to heal severe wounds. Combining polyvinyl alcohol (PVA) and silk fibroin (SF) nanofibers is a promising method of developing a skin scaffold because the resulting structure mimics collagen fibers. The aim of this research was to study the growth of human dermal fibroblasts (HDF) on a polyvinyl alcohol-silk fibroin (PVA-SF) nanofiber scaffold that was produced by electrospinning. Morphological characterization and chemical analysis of the scaffold were performed by scanning electron microscopy (SEM), Fourier transform infrared spectrophotometry (FTIR), and contact angle measurement. The biocompatibility of the scaffold was tested by MTT cytotoxicity assay, SEM analysis, adherence ratio calculation, and analysis of the HDF growth curve for 9 days. The FTIR results confirmed the presence of SF and PVA. The average fiber diameter and pore size of the PVA scaffold were greater than those of the PVA-SF scaffold. Both scaffolds had hydrophilic properties and were not cytotoxic. Thus, HDF can attach and grow on both types of scaffold better than HDF seeded on a polystyrene plate. In conclusion, the addition of SF to the PVA nanofibers caused bead formation, which affected the substrate topography, decreased hydrophilicity and also decreased the fiber diameter and pore size in the nanofiber scaffold compared to the PVA nanofiber scaffold without SF addition. SF addition increases cell attachment to the nanofiber scaffold and has potential to facilitate HDF cell growth.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73679830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Donsker’s Delta Functional of Stochastic Processes with Memory 有记忆随机过程的Donsker函数

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Journal of Mathematical and Fundamental Sciences Pub Date : 2019-12-31 DOI: 10.5614/j.math.fund.sci.2019.51.3.5

H. Suryawan

引用次数: 2

B-emission Stars in Southern Sky Observed in Bosscha Observatory: Spectroscopic Study of Hα Spectral Line Variability 博沙天文台观测到的南方天空b发射星:Hα谱线变异性的光谱研究

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Journal of Mathematical and Fundamental Sciences Pub Date : 2019-12-31 DOI: 10.5614/j.math.fund.sci.2019.51.3.3

A. Aprilia, H. Malasan, G. E. Ramadhania, A. T. P. Jatmiko, M. Irfan, D. G. Ramadhan, A. N. Istiqomah, E. I. Akbar

引用次数: 0

Cytotoxic Activities of Extracts and Isolated Compounds of Some Potential Sumatran Medicinal Plants against MCF-7 and HSC-3 Cell Lines 一些苏门答腊潜在药用植物提取物和分离化合物对MCF-7和HSC-3细胞系的细胞毒活性

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Journal of Mathematical and Fundamental Sciences Pub Date : 2019-12-31 DOI: 10.5614/j.math.fund.sci.2019.51.3.2

Meiridha Mutiara Andania, F. Ismed, M. Taher, S. Ichwan, A. Bakhtiar, D. Arbain

{"title":"Cytotoxic Activities of Extracts and Isolated Compounds of Some Potential Sumatran Medicinal Plants against MCF-7 and HSC-3 Cell Lines","authors":"Meiridha Mutiara Andania, F. Ismed, M. Taher, S. Ichwan, A. Bakhtiar, D. Arbain","doi":"10.5614/j.math.fund.sci.2019.51.3.2","DOIUrl":"https://doi.org/10.5614/j.math.fund.sci.2019.51.3.2","url":null,"abstract":"In continuation of our study of Sumatran plants and the bioactivity of their constituents, Stereocaulon halei Lamb (Stereocaulaceae) was extracted and isolated to obtain atranorin ( 1 ), lobaric acid ( 6 ), and methyl- β -orcinol carboxylate ( 9 ). We also prepared methanolic extracts of some Sumatran medicinal plants, i.e. Alpinia denticulata, Alpinia malaccensis, Alpinia submutica, Curcuma zedoaria, Centella asiatia , Mangifera indica, Piper crocatum, Mussaenda frondosa , together with previously isolated compounds, i.e. crocatin A ( 2 ) and crocatin B ( 3 ) from P. crocatum , curcumin ( 4 ) from Curcuma longa , demethoxymatteucinol ( 5 ) from Sphaerostephanos polycarpus , mangiferin ( 7 ) from M. indica , methyl caffeate ( 8 ) from Balanophora elongata , tiliroside ( 10 ) from Guazuma ulmifolia , and usnic acid from Usnea sp ( 11 ). These extracts and compounds were tested for their cytotoxicity against MCF-7 and HSC-3 cell lines by MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). It was found that the IC 50 value of atranorin ( 1 ), lobaric acid ( 6 ), methyl- β -orcinol carboxylate ( 9 ), and methanolic extract of A. submutica towards the MCF-7 cell line was 208.20 µM, 172.05 µM, 382.60 µM and 70.95 µg/mL, respectively. The IC 50 value of lobaric acid ( 6 ) and methyl-β-orcinol carboxylate ( 9 ) against the HSC-3 cell line was 88.92 µM and 260.09 µM, respectively. The other extracts did not show significant cytotoxicity.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84119065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Polyelectrolyte Complex (PEC) of the Alginate-Chitosan Membrane for Immobilizing Urease 海藻酸盐-壳聚糖膜固定化脲酶的聚电解质复合物

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Journal of Mathematical and Fundamental Sciences Pub Date : 2019-12-20 DOI: 10.5614/j.math.fund.sci.2019.51.3.8

D. Hermanto, M. Mudasir, D. Siswanta, B. Kuswandi, N. Ismillayli

引用次数: 17

The Second Hankel Determinant Problem for a Class of Bi-Univalent Functions 一类双一元函数的第二Hankel行列式问题

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Journal of Mathematical and Fundamental Sciences Pub Date : 2019-08-06 DOI: 10.5614/J.MATH.FUND.SCI.2019.51.2.8

M. H. Khani, A. Zireh, E. A. Adegani

引用次数: 2

Effects of Root Extract of Ruellia tuberosa L. on Kidneys of Diabetic Rats 秋莲根提取物对糖尿病大鼠肾脏的影响

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Journal of Mathematical and Fundamental Sciences Pub Date : 2019-08-06 DOI: 10.5614/J.MATH.FUND.SCI.2019.51.2.3

A. Safitri, A. Srihardyastutie, A. Roosdiana, A. Aulanni’am, Eva Nur Laili Octaviana

{"title":"Effects of Root Extract of Ruellia tuberosa L. on Kidneys of Diabetic Rats","authors":"A. Safitri, A. Srihardyastutie, A. Roosdiana, A. Aulanni’am, Eva Nur Laili Octaviana","doi":"10.5614/J.MATH.FUND.SCI.2019.51.2.3","DOIUrl":"https://doi.org/10.5614/J.MATH.FUND.SCI.2019.51.2.3","url":null,"abstract":"The aim of this research was to study the antidiabetic activity of root extract of Ruellia tuberosa L. on rats ( Rattus novergicus ) induced by multiple low doses of streptozotocin. The parameters investigated were blood glucose levels, protease activities, and the histopathologic profiles of the kidneys. Three groups of rats, i.e. control, diabetic, and treatment, were used. R. tuberosa L. extracts were given orally for treatment at a dose of 250 mg/kg body weight per day for 21 consecutive days. The induced diabetes group treated with root extract of R. tuberosa L. (group III) had 60.3% lower glucose levels (119 mg/dL) compared to the group with induced diabetes only. Moreover, antidiabetic activity of R. tuberosa L extract was evidenced by the 55% lower protease activity in the treatment group compared to the untreated diabetic group. Finally, a positive effect from root extract of R. tuberosa L. was shown from the less damaged renal glomerulus and smaller gap between extracellular matrices and epithelium cells in the histopathologic profiles of the kidneys in the extract treatment group. These results show the potential of root extract of R. tuberosa L. as a natural product anti-diabetic agent.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76316819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Evaluation of Batch Culture Phytoremediation Process using Local Hydromacrophytes to Reduce Synthetic Pesticide Residue in Contaminated Irrigation Water 利用当地水生植物批量培养修复污染灌溉水中合成农药残留的评价

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Journal of Mathematical and Fundamental Sciences Pub Date : 2019-08-06 DOI: 10.5614/J.MATH.FUND.SCI.2019.51.2.2

C. Retnaningdyah, E. Arisoesilaningsih

{"title":"Evaluation of Batch Culture Phytoremediation Process using Local Hydromacrophytes to Reduce Synthetic Pesticide Residue in Contaminated Irrigation Water","authors":"C. Retnaningdyah, E. Arisoesilaningsih","doi":"10.5614/J.MATH.FUND.SCI.2019.51.2.2","DOIUrl":"https://doi.org/10.5614/J.MATH.FUND.SCI.2019.51.2.2","url":null,"abstract":"The aim of this study was to evaluate the effectivity of batch culture phytoremediation to remediate irrigation water that was contaminated with 6.4 µL/L synthetic pesticide Prevathon. This was a true experimental research study using a completely randomized design, conducted in a 30 L bucket with sand and gravel as the substrate. The treatment comprised five types of hydromacrophytes (emergent, floating leaf, submerged, polyculture and control without plants), repeated three times at the same time. The effectiveness of the phytoremediation model was determined by water physicochemical parameters, periphyton diversity, percentage of pollution tolerant values (%PTV) and trophic diatom index (TDI) on day 6, 13, 29 and 37 after incubation. The research results showed significantly decreased values of biochemical oxygen demand, turbidity, bicarbonate, nitrate, orthophosphate, total phosphate and free chlorine after incubation for 6 days; decreased concentration of chemical oxygen demand after incubation for 13 days. The water quality improved from slightly and moderately polluted (diversity index 1.6-2.8) to clean and slightly polluted (diversity index 2.8-4.2), from moderately and heavily organically polluted (PTV 40.0-93.7%) to slightly organically polluted and free of organic pollution (PTV 2.4-34.1%), and from meso-eutrophic and eutrophic (TDI 37.4-70.4) to oligo and meso-eutrophic (TDI 13.7-26.4).","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83638352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

P-properties in Near-rings 近环中的p -性质

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Journal of Mathematical and Fundamental Sciences Pub Date : 2019-08-06 DOI: 10.5614/J.MATH.FUND.SCI.2019.51.2.5

A. O. Atagün, Hüseyin Kamacı, İsmail Taştekin, A. Sezgi̇n

引用次数: 2