螺旋藻藻蓝色素提取方法优化及性质研究

IF 0.5 Q4 MULTIDISCIPLINARY SCIENCES

Journal of Mathematical and Fundamental Sciences Pub Date : 2019-08-06 DOI:10.5614/J.MATH.FUND.SCI.2019.51.2.6

E. Julianti, S. Susanti, M. Singgih, Laida Neti Mulyani

{"title":"螺旋藻藻蓝色素提取方法优化及性质研究","authors":"E. Julianti, S. Susanti, M. Singgih, Laida Neti Mulyani","doi":"10.5614/J.MATH.FUND.SCI.2019.51.2.6","DOIUrl":null,"url":null,"abstract":"Spirulina platensis is a microalgae species that contains phycocyanin, a blue pigment that is widely used in the food, cosmetic and pharmaceutical industries. The objective of this study was to isolate phycocyanin from dried biomass of Spirulina platensis. The optimum extraction was through freeze-thawing extraction with 0.01 M phosphate buffer pH 7. Purification was done using 50% ammonium sulphate precipitation, dialysis, and gel filtration chromatography. The purity ratio of the purified product was 1.729 (A615/A280). Characterization of isolate, carried out using SDS-PAGE, gave molecular weights of around 15-16 Kda and using infrared spectrophotometry the similarity with the standard phycocyanin spectrum was obtained. Both methods confirmed that the isolate was phycocyanin.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"19 1","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"14","resultStr":"{\"title\":\"Optimization of Extraction Method and Characterization of Phycocyanin Pigment from Spirulina platensis\",\"authors\":\"E. Julianti, S. Susanti, M. Singgih, Laida Neti Mulyani\",\"doi\":\"10.5614/J.MATH.FUND.SCI.2019.51.2.6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Spirulina platensis is a microalgae species that contains phycocyanin, a blue pigment that is widely used in the food, cosmetic and pharmaceutical industries. The objective of this study was to isolate phycocyanin from dried biomass of Spirulina platensis. The optimum extraction was through freeze-thawing extraction with 0.01 M phosphate buffer pH 7. Purification was done using 50% ammonium sulphate precipitation, dialysis, and gel filtration chromatography. The purity ratio of the purified product was 1.729 (A615/A280). Characterization of isolate, carried out using SDS-PAGE, gave molecular weights of around 15-16 Kda and using infrared spectrophotometry the similarity with the standard phycocyanin spectrum was obtained. Both methods confirmed that the isolate was phycocyanin.\",\"PeriodicalId\":16255,\"journal\":{\"name\":\"Journal of Mathematical and Fundamental Sciences\",\"volume\":\"19 1\",\"pages\":\"\"},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2019-08-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"14\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Mathematical and Fundamental Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5614/J.MATH.FUND.SCI.2019.51.2.6\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Mathematical and Fundamental Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5614/J.MATH.FUND.SCI.2019.51.2.6","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}

引用次数: 14

摘要

螺旋藻是一种含有藻蓝蛋白的微藻，藻蓝蛋白是一种蓝色色素，广泛用于食品、化妆品和制药行业。本研究的目的是从干燥的螺旋藻生物量中分离藻蓝蛋白。以0.01 M pH为7的磷酸盐缓冲液进行冻融提取，提取效果最佳。采用50%硫酸铵沉淀、透析和凝胶过滤层析进行纯化。纯化产物的纯度比为1.729 (A615/A280)。利用SDS-PAGE对分离物进行了表征，得到了15-16 Kda左右的分子量，并利用红外分光光度法获得了与标准藻蓝蛋白相似的光谱。两种方法均证实该分离物为藻蓝蛋白。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Optimization of Extraction Method and Characterization of Phycocyanin Pigment from Spirulina platensis

Spirulina platensis is a microalgae species that contains phycocyanin, a blue pigment that is widely used in the food, cosmetic and pharmaceutical industries. The objective of this study was to isolate phycocyanin from dried biomass of Spirulina platensis. The optimum extraction was through freeze-thawing extraction with 0.01 M phosphate buffer pH 7. Purification was done using 50% ammonium sulphate precipitation, dialysis, and gel filtration chromatography. The purity ratio of the purified product was 1.729 (A615/A280). Characterization of isolate, carried out using SDS-PAGE, gave molecular weights of around 15-16 Kda and using infrared spectrophotometry the similarity with the standard phycocyanin spectrum was obtained. Both methods confirmed that the isolate was phycocyanin.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Mathematical and Fundamental Sciences MULTIDISCIPLINARY SCIENCES-

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

24 weeks

期刊介绍： Journal of Mathematical and Fundamental Sciences welcomes full research articles in the area of Mathematics and Natural Sciences from the following subject areas: Astronomy, Chemistry, Earth Sciences (Geodesy, Geology, Geophysics, Oceanography, Meteorology), Life Sciences (Agriculture, Biochemistry, Biology, Health Sciences, Medical Sciences, Pharmacy), Mathematics, Physics, and Statistics. New submissions of mathematics articles starting in January 2020 are required to focus on applied mathematics with real relevance to the field of natural sciences. Authors are invited to submit articles that have not been published previously and are not under consideration elsewhere.