Neonatology, surgery and perinatal medicine最新文献

{"title":"PSYCHOSOMATIC MECHANISMS OF REGULATION OF EXTERNAL RESPIRATION FUNCTION IN CHILDREN WITH BRONCHIAL ASTHMA WHEN LISTENING TO AUDIO SIGNALS OF VARIOUS COMPONENT COMPOSITION","authors":"L. Kalashnikova, S. Naida, V. Didkovskyi, V. Zaets, S. Bartenev","doi":"10.24061/2413-4260.xiii.4.50.2023.8","DOIUrl":"https://doi.org/10.24061/2413-4260.xiii.4.50.2023.8","url":null,"abstract":"In the treatment of bronchial asthma (BA), an integrated approach is used that includes both drugs and alternative methodsthat infl uence the psycho- emotional state of the patient. In this regard, one of the most powerful methods of psycho- emotional infl uence on a person is musical infl uence.The aim of the research is to study the eff ectiveness of infl uence of audio signals of diff erent amplitude- frequency range on the central mechanisms of regulation of ventilatory capacity of bronchi of patients with asthma.Material and methods. Twenty-fi ve children between the ages of 9 and 14 with clinically diagnosed BA participated in thestudy. Three melodies with diff erent component composition were used. Their amplitude- frequency characteristics were analyzed using the Matlab environment. The sound energy fraction of the studied musical compositions in one-third octave frequency bands throughout the duration of the audio signal was calculated. Statistical processing of the data was performed using IBM SPSS Statistics software.Results. The results of the study indicate that the most eff ective musical infl uence is provided by the audio excerpt from The Magic Flute by W. A. Mozart. It was found that the main spirographic indicators of bronchopulmonary obstruction increase under the infl uence of music, and correspondingly there are changes in the integral characteristics of bioelectrical activity of the brain.Conclusion. The results of the study show reliable normalization of spirography indicators in children with bronchial asthmaunder the infl uence of selected musical melodies of a certain frequency. Eff ectiveness of sound infl uence on central regulation mechanisms of bronchial ventilatory capacity can be used for preventive and therapeutic purposes.","PeriodicalId":162458,"journal":{"name":"Neonatology, surgery and perinatal medicine","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139313661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STUDY OF THE CIRCADIAN MOLECULAR CLOCK IN PAEDIATRIC PATIENTS WITH SEASONAL ALLERGIC RHINITIS: POTENTIAL APPROACHES TO TREATMENT","authors":"T. Kryuchko, V. Shcherbak, L. Bubyr, O. Izmailova, O. Tkachenko","doi":"10.24061/2413-4260.xiii.4.50.2023.6","DOIUrl":"https://doi.org/10.24061/2413-4260.xiii.4.50.2023.6","url":null,"abstract":"Understanding of the relationship between the circadian molecular clock and allergic diseases continues to be studied and may be of practical importance for further selection of optimal treatment. The aim of this study was to evaluate the degree of mRNA expression of the circadian molecular clock genes bmal1 and per1 in buccal epithelial cells and to investigate the effi cacy of protocol treatment in children with seasonal allergic rhinitis, taking into account the time dependence. Material and methods. The study included 20 patients aged 6 to 17 years with a confi rmed diagnosis of seasonal allergic rhinitis (SAR). The control group included 7 healthy children of the same age with no history of allergic or chronic diseases. All patients with SAR were divided into 2 groups of 10 patients each, who received the protocol therapy in the morning (group 1) and in the evening (group 2) for 4 weeks. The expression of circadian molecular clock genes was studied by real-time polymerase chain reaction (real-time PCR) method by sampling the buccal epithelium of the children’s oral cavity in the morning (8:00 am) and in the evening (8:00 pm), with a repeat examination of the samples 1 month after treatment.Statistical analysis was performed using the GraphPad Prism 5.00 computer program (GraphPad Software, Inc., San Diego, CA, USA). The research design was discussed and approved at the meeting of the medical and ethical commission of the Ukrainian Medical and Stomatological Academy, protocol No. 188 dated 11/25/2020, where permission to conduct medical and biological research was issued. The research design was discussed and approved at the meeting of the Medical and Ethical Committee of the Ukrainian Academy of Medicine and Dentistry, Protocol No. 188 dated 25.11.2000, where permission to conduct medical and biological research was granted.The study was conducted in accordance with the research plan of the Pediatrics Department no. 2 of Poltava State MedicalUniversity «Optimization of early diagnosis, treatment and prognosis of the most common childhood diseases», state registration number 0122U001876 (years of implementation 2021-2026) and research work – Research Institute of genetic and immunological bases of development of pathology and pharmacogenetics of PDMU «Development of methods of treatment and prevention of development of pulmonary fi brosis by activation of PPAR-gamma receptors», state registration number 0122U201686 (years of implementation 2023-2025).Results. Morning gene expression in healthy children showed statistically higher levels of per1 (0.780 ± 0.070) compared to bmal1 (0.293 ± 0.074; p<0.001), whereas in the evening the level of the latter (0.697 ± 0.130) was signifi cantly higher than per1 (0.370 ± 0.044; p<0.05). Comparing the levels of per1 in this group, the expression of this gene was higher in the morning (0.780 ± 0.070) than in the evening (0.370 ± 0.044; p<0.001), while the expression of bmal1 was higher at 20:00 (0.697 ± 0.","PeriodicalId":162458,"journal":{"name":"Neonatology, surgery and perinatal medicine","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139313173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BREAST MILK BANKS – THE WAY TO GLOBAL BREASTFEEDING SUPPORT IN UKRAINE AND THE WORLD","authors":"M. Malachynska, M. Kiselova, O. Shlemkevych, N. Veresnyuk, N. Kunta","doi":"10.24061/2413-4260.xiii.4.50.2023.2","DOIUrl":"https://doi.org/10.24061/2413-4260.xiii.4.50.2023.2","url":null,"abstract":"Breast milk is the standard of infant nutrition, recognized worldwide as the optimal fi rst source of food, and breastfeedingis the ideal way to feed children, meeting all their needs. Breast milk is especially valuable for premature infants, who require specialized medical care from the fi rst seconds of life and for a long time, and for sick newborns born at term. Preterm or sick preterm infants are most vulnerable to perinatal illness, have a high probability of dying and, most importantly, are at the greatest risk of not receiving the life-saving benefi ts of breast milk. In today’s world, the World Health Organization and the American Academy of Pediatrics recommend the use of donor human milk as the fi rst alternative for feeding preterm infants of any gestational age, including very preterm infants (<32 weeks gestation) or very low birth weight infants (<1500 g), when human milk is not available. Studies have shown that breastfeeding, even partial breastfeeding, reduces the risk of necrotizing enterocolitis and late neonatal sepsis, retinopathy of prematurity, and sudden infant death, improves cognitive outcomes in preterm infants, and helps establish optimal functioning of the digestive system and enteric digestive processes. In many countries around the world, targeted support for breastfeeding in neonatal intensive care units, including enteral feeding of sick and premature infants, is provided by donor human breast milk that is concentrated and stored in an established and functioning network of breast milk banks.A breast milk bank is an institution whose purpose is to collect breast milk from mothers who produce more than theirinfants need, and to process and store breast milk for the feeding of infants who, for medical or other reasons, cannot receive it temporarily or for a long period. International associations of breast milk banks have been established throughout the world to promote the activities of banks and to establish international cooperation among breast milk banks.The European Milk Banks Association (EMBA) was offi cially launched on October 15, 2010. There are currently 282 activebanks in 31 European countries and 18 banks in the process of becoming active. The Southern African Milk Banks Association (HMBASA) was established in 2009. HMBASA coordinates the activities of the network of milk banks throughout the country. The North American Milk Banks Association (HMBANA) was founded in 1985 and brings together 30 non-profi t breast milk banks in the United States and Canada. There is a positive trend in the establishment of new breast milk banks in all countries of the world. There are currently about 500 in 37 countries. Brazil is the world leader in breast milk banks, with 222 breast milk banks and 217 milk collection centers. The first donor breast milk bank in Ukraine was established in 2019 in Kyiv at the Perinatal Center, where about 80 % of all premature babies in the city are born. In December 2022, when the count","PeriodicalId":162458,"journal":{"name":"Neonatology, surgery and perinatal medicine","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139313505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BODY COMPOSITION OF FULL-TERM NEWBORNS AGAINST THE BACKGROUND OF INCREASED PROTEIN INTAKE IN THE INTENSIVE CARE UNIT","authors":"I. Anikin, L. Stryzhak","doi":"10.24061/2413-4260.xiii.4.50.2023.4","DOIUrl":"https://doi.org/10.24061/2413-4260.xiii.4.50.2023.4","url":null,"abstract":"To evaluate the eff ects of increased protein and L-carnitine supplementation in the feeding program of term infants withperinatal conditions on improving physical development outcomes, changes in body composition and hospital outcomes.The aim is to evaluate the eff ect of short-term increased protein intake in term infants and L-carnitine supplementation onbody composition and key indicators of physical development in children.Material and methods. To test the proposed hypothesis of neonatal nutrition, we studied the vital signs of 59 term infantsrandomized into two groups. The fi rst group (n = 30) received standard breastmilk (BM) or formula feeding, and the second group (n = 29) received a protein- fortifi ed (PF) formula and L-carnitine supplementation during the hospital stay. At the beginning and end of treatment, the children’s physical development and body composition were assessed using bioelectrical impedance.The study protocol was agreed and approved by the Regional Bioethics Committee of the Zaporizhzhia State Medical andPharmaceutical University. The study was conducted in accordance with the moral and ethical standards of the IGH/GCP, theDeclaration of Helsinki (1964 with amendments of 1975, 1983, 1989, 1996, 2000), the Convention of the Council of Europe onHuman Rights and Biomedicine, and the legislation of Ukraine. Written informed consent was obtained from the parents of the patients before the start of the study. All statistical analyses were performed using the software Statistica 13.0, TIBCO Software Inc (licence number JPZ804I382130ARCN10-J) and Microsoft Excel 2013 (licence number 00331-10000-00001-АА404). The probability of the diff erence in the absolute values of the means was determined using non-parametric methods of statistical analysis: the Mann-Whitney test (U) for unrelated groups and the Wilcoxon signed rank test (T) for related groups. Statistical signifi cance was defi ned as p < 0.05. The study was conducted within the framework of the research work of the Department of Anaesthesiology and Intensive Care Medicine of the Zaporizhzhia State Medical and Pharmaceutical University of the Ministry of Health of Ukraine – «Optimization of diagnosis and intensive care of multi etiological lesions of the brain, gastrointestinal tract, kidneys in newborns and older children», state registration number 0118U007142.Results. Infants in both groups had similar characteristics of weight, length and head circumference at baseline. Theproportions of infants of both sexes were within the 50 % percentile. In general, the proposed feeding strategy contributed to better indicators of infant physical development and a statistically signifi cant, faster recovery of body weight in the fortifi ed group. Thus, the generalised indicator of body weight of children in the GZ group was 3966.90 ± 439.08 g, compared to 3554.62 ± 452.28 g in the SC group, p = 0.0033. In general, the children who consumed more protein were transferred out of in","PeriodicalId":162458,"journal":{"name":"Neonatology, surgery and perinatal medicine","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139313724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SARS-COV-2 AND HYPERCOAGULATION IN PREGNANT WOMEN","authors":"A. Boychuk, Y. Yakymchuk, O. Yakymchuk","doi":"10.24061/2413-4260.xiii.4.50.2023.14","DOIUrl":"https://doi.org/10.24061/2413-4260.xiii.4.50.2023.14","url":null,"abstract":"The global pandemic caused by the SARS-CoV-2 virus has created unprecedented medical and social problems. The virus has the ability to induce a prothrombotic state, which in certain cases has led to severe thrombotic complications. Scientists all over the world were faced with the task of studying in detail the coagulation capacity of blood plasma and the procoagulant role of platelets in the development of pathogenic mechanisms of endothelial damage, major vascular thrombosis and systemic microangiopathies. These studies will be useful for clinicians to improve pathogenetic- based treatment schemes for this disease and to prevent serious complications. The aim of the study was to determine the eff ect of the SARS-CoV-2 virus on some parameters of the blood coagulation system in pregnant women with post-covid syndrome.Method and materials. From November 2020 to January 2022, we conducted a prospective cohort study of 50 pregnant women (main group) with SARS-CoV-2 confi rmed by the polymerase chain reaction method. The control group consisted of 25 women with physiological pregnancy who were undergoing inpatient treatment at the communal non-profi t enterprise «Ternopil City Communal Hospital No. 2». The conduct of this study was approved by the Ethics Committee of Ternopil National Medical University named after I. Gorbachevsky – protocol No. 61 dated November 13, 2020. Pregnant women were examined between 30 and 34 weeks of gestation. In the selected blood samples, the number of platelets, coagulogram indicators: international normalized ratio, prothrombin and thrombin time, prothrombin index, activated partial thromboplastin time, fi brinogen and D-dimer levels were studied. The studies were performed on a Coag Chrom 3003 analyzer. Microsoft Excel and Statistica-10 software were used for statistical analysis. The study was conducted within the framework of the National Development Program «Improvement of diagnosis and treatment of pregnant women with a burdened somatic history» of the Department of Obstetrics and Gynecology, Faculty of Postgraduate Education, TernopilNational Medical University named after I. Ya. Gorbachevsky, state registration number N 0121U100153, performance period 2021-2023).Results. A signifi cant decrease in the absolute platelet count to less than 100,000/μL was observed in 5 % of the pregnant women in the main group, and in 70 % of the patients in this group, the platelet count was less than 150,000/μL, and only 25 % of the pregnant women in this group had platelet counts within the normal range. In the control group, only 16 % of the platelets were in the 150-180 range, and 84 % of the pregnant women in this group had platelet counts within the normal range. Prothrombin time indicators were slightly longer in patients with COVID-19 (15.6 s; 14.4-16.3) compared with the control group (13.6 s; 13.0-14.3), (Р>0.05). In pregnant women with SARS-CoV-2, the concentration of fi brinogen was 1.63 times higher than in the cont","PeriodicalId":162458,"journal":{"name":"Neonatology, surgery and perinatal medicine","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139313589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CHANGES IN CYTOKINE BALANCE IN PREGNANT WOMEN WITH CHRONIC ENDOMETRITIS IN THE PAST MEDICAL HISTORY AND THEIR ROLE IN THE DEVELOPMENT OF PREECLAMPSIA","authors":"V. Likhachov, O. Taranovska","doi":"10.24061/2413-4260.xiii.4.50.2023.16","DOIUrl":"https://doi.org/10.24061/2413-4260.xiii.4.50.2023.16","url":null,"abstract":"Few data regarding the levels of TNF-α, INF-γ and IL-10 in the cervical mucus of pregnant women with the past medicalhistory of chronic endometritis have been found. The abovementioned cytokines have the impact on the course of pregnancy as well as the processes of trophoblast invasion into the spiral arteries of the uterus. Deviations in their levels at the early stages of pregnancy may be associated with the development of preeclampsia at later stages.Purpose: to assess the levels of TNF, INF-γ and cytokine IL-10 in the cervical mucus of pregnant women with a past medicalhistory of chronic endometritis. Additionally, the study aims to determine their role in the development of preeclampsia andevaluate the eff ectiveness of comprehensive preconception treatment in preventing cytokine imbalance and the occurrence of gestational complications.Method and Materials. 135 pregnant women with the past medical history of chronic endometritis have been supervisedduring their pregnancy and received preconception treatment (Group I), as well as 168 women, whose pregnancy occurred in the presence of untreated chronic endometritis (Group II). The control group (CG) involved 20 healthy women. Preconception treatment included the administration of azithromycin, hormonal therapy with Femoston- Comb 2/10, and L-arginine aspartate in combination with folate prophylaxis. During the study, the principles of patient- centered care were followed in accordance with the requirements of the Tokyo Declaration of the World Medical Association, the International Recommendations of the Helsinki Declaration on Human Rights, the Convention on Human Rights and Biomedicine of the Council of Europe, the Laws of Ukraine, the Orders of the Ministry of Health of Ukraine and the requirements of the Ethical Code of the Ukrainian physician. The cytokine (TNF-α, INFγ, IL-10) analysis in the cervical mucus was performed at 5-, 17-18 and 32 weeks of pregnancy, using the enzyme- linked immunosorbent assay method with corresponding standard commercial reagent kits from the «Vector BEST» company. The obtained data were processed using mathematical statistical methods, calculating the mean sample values (M), variance (σ) and standard errors of the means (m). Student’s t-test was used for assessment and the likelihood ratio was calculated using the «STATISTICA» software (StatSoft Inc., USA).The paper is an excerpt from the initiative scientifi c research project of the Department of Obstetrics and Gynecology No.2 at Poltava State Medical University, entitled «Optimization of approaches to the management of pregnancy in women at high risk of obstetric and perinatal pathology» (State registration number 0122U201228, duration:10.2022-09.2027).Results. In pregnant women with a past medical history of chronic endometritis an increase in the levels of INF-γ in cervicalmucus by 2.1 times at 5-6 weeks (p<0.001), by 2.4 times at 17-18 weeks (p<0.001) and by 1.7 times at 32 weeks of pregnancy(p<0.001) ","PeriodicalId":162458,"journal":{"name":"Neonatology, surgery and perinatal medicine","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139313212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PECULIARITIES OF THE IDENTIFICATION OF DIFFERENT TYPES OF TISSUES DURING 3D-RECONSTRUCTION OF HUMAN MICROSCOPIC STRUCTURES","authors":"R. Dmytrenko, O. Koval, L. Andrushchak, I. Makarchuk, O. Tsyhykalo","doi":"10.24061/2413-4260.xiii.4.50.2023.18","DOIUrl":"https://doi.org/10.24061/2413-4260.xiii.4.50.2023.18","url":null,"abstract":"3D reconstruction is an informative, objective method of morphological research that consists in transforming a seriesof successive sections (histological, macroscopic, anatomical sections, computed tomography (CT), etc.) into a virtual threedimensional (digital) image that can be studied in diff erent projections and measure volumes, areas, diameters, angles, save, copy, edit.The aim of the study. The aim of this work was to compare the eff ectiveness of 3D reconstruction methods of various tissuesand microscopic anatomical structures of the human body in the prenatal period of development.Material and methods. The research was carried out on 6 series of consecutive histological sections of human embryos atthe age of 4 to 6 weeks of intrauterine development (IUD), 15 samples of organ complexes of the head, limbs and trunk of human fetuses at the age of 7 to 12 weeks of IUD, human fetuses aged 4-9 months of IUD by the method of making histological (5), as well as histotopographic sections (10) directly from the paraffi n block and their digitization, and 14 CT of human fetuses aged 4 to 9 months of IUD. The studies were conducted in accordance with the most important regulations of the resolution of the First National Bioethics Congress «General Ethical Principles of Experiments on Animals» (2001), ICH GCP (1996), the European Union Convention on Human Rights and Biomedicine (April 4, 1997) and the European Convention for the Protection of Vertebrate Animals used in Experimental and Other Scientifi c Research (March 18, 1986). 1997) and the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientifi c Purposes (18.03.1986), the Declaration of Helsinki on Ethical Principles for Medical Research Involving Human Subjects (1964-2008), EU Directives № 609 (24.11.1986), Orders of the Ministry of Health of Ukraine № 690 dated 23.09.2009, № 944 dated 14.12.2009, № 616 dated 03.08.2012. The work is carried out within the framework of the initiative research work of the Department of Histology, Cytology and Embryology of the Bukovinian State Medical University «Structural and functional peculiarities of tissues and organs in ontogenesis, regularities of variant, constitutional, gender, age and comparative human morphology». State registration number: 0121U110121. Terms of execution: 01.2021-12.2025.Results. 3D reconstruction of series of consecutive histological sections is eff ective for the study of embryonic specimens,organ complexes of prefetuses and certain microscopic structures of human fetuses due to easy identifi cation of histological structures, however, it is necessary to improve the methods of alignment of histological sections both in the correct sequence of the series and in the natural position. 3D reconstruction of histotopographic sections is recommended for the study of specimens of organ complexes of human prefetuses and fetuses. 3D reconstruction of CT sections is an eff ective and highly accu","PeriodicalId":162458,"journal":{"name":"Neonatology, surgery and perinatal medicine","volume":"223 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139313419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CHARACTERISTICS OF THE GINGIVAL EPITHELIUM OF CHILDREN’S THIRD MOLARS AT DIFFERENT STAGES OF ROOT FORMATION","authors":"T. Muryniuk","doi":"10.24061/2413-4260.xiii.4.50.2023.19","DOIUrl":"https://doi.org/10.24061/2413-4260.xiii.4.50.2023.19","url":null,"abstract":"Extraction of impacted and dystopic third molars is a rather traumatic manipulation accompanied by a large bone defect and wound infection. Therefore, it is advisable to perform hermectomy, which has a number of advantages over typical and atypical methods of tooth extraction. An important stage of any surgical intervention is the postoperative period, which is characterized by a certain duration and the presence of complications, which is due, among other things, to the regenerative potential of tissues, especially epithelial tissues. Aim of the study is to evaluate the condition of the gingival epithelium in the area of third molars in children who have to have these teeth extracted for orthodontic reasons, taking into account the stage of tooth formation, i. e. its root.Material and Methods. Third molar extraction for orthodontic indications was performed in 95 children aged 11 to 18 years.Clinical and radiologic examinations were performed in three groups: Group I (n=30) – children aged 11-13 years; Group II(n=35) – children aged 13-16 years, and Group III (n=30) – children aged 16-18 years. During surgery, gingival tissue was taken from the adjacent areas for examination. The material was fi xed, dehydrated, and paraffi n- embedded for histologic processing. Immunohistochemical techniques were performed according to the manufacturer’s protocols. Specifi cally, immunohistochemical detection of Ki-67 antigen was performed with primary antibodies against it. Digital copies of the images were analyzed using the specialized computer program ImageJ v1.52 (freeware, USA). The obtained digital data were statistically processed.The digital data obtained were statistically processed. A preliminary test for normality of the distribution was carried outusing the Shapiro- Wilks method. According to this criterion, the hypothesis of normality of the distribution was not rejectedfor all the statistical samples studied (at the signifi cance level of p=0.05), so we used mainly parametric methods of statistical analysis: calculation of the arithmetic mean and its error, unpaired two-sided Student’s t-test. However, because the statistical samples were small, the non-parametric Mann- Whitney test was used in addition to the Student’s t-test.The study was conducted as part of the complex research work of the Department of Pediatric Dentistry of the Bukovinian State Medical University on the topic «Development of methods of prevention and treatment of the main dental diseases in children, taking into account the risk factors of their development» (state registration number: 0121U110122). The conducted research complied with bioethical norms, as stated in the conclusions of the Bioethics Commission of the Bukovinian State Medical University (№ 1 dated 17.09.2020).Conclusions. Thus, the histological and immunohistochemical study of the gingiva in the area of the third molar allows usto conclude that during the formation of the root of this tooth, a number of chang","PeriodicalId":162458,"journal":{"name":"Neonatology, surgery and perinatal medicine","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139313621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CLINICAL AND LABORATORY CHANGES IN POSTSURGICAL PAIN MARKERS IN CHILDREN","authors":"Semkovych Ya.V","doi":"10.24061/2413-4260.xiii.4.50.2023.13","DOIUrl":"https://doi.org/10.24061/2413-4260.xiii.4.50.2023.13","url":null,"abstract":"Pos toperative pain in children remains a signifi cant problem in pediatric intensive care, possibly related to an imperfectalgorithm for its management. Prolonged postoperative pain delays recovery and rehabilitation, increases treatment costs,and prolongs opioid use. Inadequate perioperative pain management can lead to a variety of postoperative complications, the prediction of which remains elusive despite the use of commonly accepted clinical, laboratory, and instrumental indicators. The aim of this study was to evaluate clinical and laboratory changes in children after abdominal surgery and their correlation with acute pain scales.Material and Methods. The study included 83 children aged 7-18 years who underwent abdominal surgery under opioid anesthesia. Inclusion criteria: age 7-18 years; indication for surgery for acute appendicitis; American Society of Anesthesiologists (ASA) Anesthesia Risk Score I and II; parental consent for the children to participate in the study.Acute pain intensity was measured using the Visual Analog Scale (VAS) and the Face, Legs, Activity, Cry, Consolability(FLACC) scale. Key vital signs assessed included heart rate, respiratory rate, systolic and diastolic blood pressure, and oxygen saturation (SpO2). In addition, laboratory indicators including leukocyte count, blood glucose level, erythrocyte sedimentation rate, and TLR-4 and CD40L levels were determined. All clinical and laboratory studies were conducted in accordance with the World Medical Association Declaration of Helsinki«Ethical Principles for Medical Research Involving Human Subjects» (Minutes of the Ethics Committee No. 2, dated February 24, 2002). Statistical analysis was performed on a personal computer using Statistica 10 software, using parametric and nonparametric statistical methods. The study is a part of the research project of the Department of Children’s Diseases of the Postgraduate Medical Education Faculty of the Ivano- Frankivsk National Medical University «Health Status and Adaptation of Children from the Precarpathian  Region with Somatic Diseases, Their Prevention» 2021-2026, state registration number 0121U111129; the author is a co-researcher.Results. The mean age of the children was 13.8±0.23 years, while the mean body weight was 40.9±1.6 kg. Gender assessment showed no diff erences between boys and girls (55.4 % and 44.6 %, respectively, р>0.05).The study showed very strong positive correlations (r=0.9-1.0) between acute pain assessment scores on the VAS and FLACCscale and heart rate, blood glucose levels; strong positive correlations (r=0.7-0. 9) with total leukocyte count and erythrocytesedimentation rate; moderate positive correlations (r=0.5-0.7) with serum TLR4 and CD40L levels; weak positive correlations(r<0.5) with diastolic blood pressure, SpO2, and opioid and non-opioid analgesic administration.Conclusions. The observed positive correlations between acute pain scores on the VAS and FLACC scale and proinflammatory indicators","PeriodicalId":162458,"journal":{"name":"Neonatology, surgery and perinatal medicine","volume":"266 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139313720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FEATURES OF NEUROPROTECTIVE TREATMENT OF CHILDREN WITH PRIMARY CONGENITAL GLAUCOMA","authors":"Z. Nazirova, D. Turakulova","doi":"10.24061/2413-4260.xiii.4.50.2023.11","DOIUrl":"https://doi.org/10.24061/2413-4260.xiii.4.50.2023.11","url":null,"abstract":"Normalization of intraocular pressure is an indispensable condition, but it does not guarantee stabilization of the glaucomatous process. Neuroprotective therapy aimed at maintaining the active function of axons of ganglion cells is of great importance. The aim of the study was to study the eff ectiveness of the neuroprotective eff ect of the Cortexin drug in congenital glaucoma.Material and methods. Thirty-two (64 eyes) children with congenital glaucoma in the compensation stage were includedin the study. Of these, 18 children (36 eyes) were included in the main group that received the neuroprotectant Cortexin. The remaining 14 children (28 eyes) were included in the control group and received standard treatment. Research methods: Visometry, ophthalmoscopy, perimetry, tonometry, tonography, ocular ultrasound, gonioscopy.Results. All patients underwent antiglaucomatous surgery and, in the absence of negative dynamics during the year, neuroprotective treatment with Cortexin was performed every three months. To study visual acuity in young children, a computer program was developed and used to determine visual acuity. The computer program «Scale for assessment of visual functions» was certifi ed (No. DGU 11841). Next, the parents of the child were explained how to score. After 1 month and after 3 months of treatment, the parents fi lled in the table and deducted points. After completion of neuroprotective treatment within one year, we collected data from the questionnaire fi lled out by the parents and analyzed visual acuity. Data analysis in the main group showed an increase in visual acuity in all stages of glaucoma except the fi nal stage. In children in the control group, visual acuity before surgery was identical to that in the main group. After one year of observation, the increase in visual acuity in the control group was much less than in the main group.Conclusion. Thus, the dynamics of visual acuity improvement was signifi cantly more pronounced in the main group, whichindicates the usefulness of including drugs with neuroprotective eff ect in the complex treatment of glaucomatous neuropathy.","PeriodicalId":162458,"journal":{"name":"Neonatology, surgery and perinatal medicine","volume":"158 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139313722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}