Journal of Investigative Surgery最新文献

{"title":"Mechanical Bowel Preparation Can Be Omitted in the Modern Era of Minimally Invasive Gynaecological Surgery and ERAS Pathways","authors":"C. Iavazzo, I. Gkegkes","doi":"10.1080/08941939.2022.2084190","DOIUrl":"https://doi.org/10.1080/08941939.2022.2084190","url":null,"abstract":"Dear Editor, With great deal of interest, we read the article entitled “The effect of mechanical bowel preparation on the surgical field in laparoscopic gynecologic surgeries: A prospective randomized controlled trial” by Ozturk et al. [1] The study evaluates the effects of mechanical bowel preparation (MBP) prior to laparoscopic gynecologic operation and whether it can offer advantages intraoperatively. As already discussed, MBP is discouraged in such operations by ERAS guidelines [2]. It should be highlighted that MBP was applied for two days prior to surgery. A fact that could raise the cost and patient’s dissatisfaction. Moreover, the evaluation of the intraoperative visualization of the surgical field, intestinal load and overall case of surgery were based on the subjective opinion of an experienced laparoscopic surgeon, and this is similar to other studies, using for example the Likert scale [3]. A recent literature review showed that MBP of any type seems to offer no advantage to any of the above mentioned intraoperative parametres [4]. Another metanalysis proposed that MBP should be abandoned prior to benign laparoscopic gynecological operations [5]. Furthermore, in the subgroup analysis of your study, no significant differences were identified in either patients with malignant diseases or those with high body mass index. Such a finding is crucial and highlights that there is no role of MBP in gynecologic laparoscopy independent of the difficulty of the planned surgery. Although, the findings of your study as well as other studies on the field are not supportive for MBP use, we should also mention that there is a gap between guidelines and reality/current medical practise as the approach is based on surgeon’s preference worldwide [6]. Nevertheless, the role of MBP could be debated by some surgeons in their contemporary practise, based on the existing data, we recommend that MBP could be abandoned in the modern era of minimally invasive gynecologic surgery and enhanced recovery pathways. Once again, we would like to thank the authors for their excellent contribution. Data availability statement","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"35 1","pages":"1609 - 1609"},"PeriodicalIF":1.9,"publicationDate":"2022-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42326183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does Arterialization of Portal Vein Have Any Effects in Large-for-Size Liver Transplantation? Hemodynamic, Histological, and Biomolecular Experimental Studies.","authors":"Rafael Rodrigues Torres,&nbsp;Ana Cristina Aoun Tannuri,&nbsp;Suellen Serafini,&nbsp;Alessandro Belon,&nbsp;Josiane Oliveira Gonçalves,&nbsp;Celso di Loreto,&nbsp;Uenis Tannuri","doi":"10.1080/08941939.2021.2021333","DOIUrl":"https://doi.org/10.1080/08941939.2021.2021333","url":null,"abstract":"<p><strong>Background: </strong>In pediatric liver transplantation, the optimal size of the transplanted liver ranges between 0.8% and 4.0% of the recipient's weight. Sometimes, the graft weight exceeds this upper limit, characterizing the large-for-size condition potentially associated with reduced blood flow and worsening of ischemia-reperfusion injury. Therefore, it would be beneficial to increase the portal flow through arterialization of the portal vein. <b>Materials and methods:</b> Fifteen pigs underwent large-for-size liver transplants. They were divided into two groups: control (CTRL 6 animals - conventional technique) and arterialization - a shunt was established between the portal vein and the splenic artery (ART 9 animals). Hemodynamic, biochemical, histological, and molecular variables were compared. <b>Results</b>: Arterialization resulted in a significant increase in portal vein pressure but no changes in other hemodynamic variables, as shown in the analysis of variance. It was observed lower ALT values (<i>p</i> = 0.007), with no differences regarding the values of blood pH and lactate (<i>p</i> = 0.54 and <i>p</i> = 0.699 respectively) or histological variables (edema, steatosis, inflammation, necrosis, and IRI - <i>p</i> = 1.0, <i>p</i> = 0.943, <i>p</i> = 0.174, <i>p</i> = 0.832, <i>p</i> = 0.662, respectively). The molecular studies showed significantly increased expression of IL6 after 3 hours of reperfusion (<i>p</i> = 0.048) and decreased expression of ICAM immediately after reperfusion (<i>p</i> = 0.03). The regression analysis suggested a positive influence of portal flow and pressure on biochemical parameters. <b>Conclusion</b>: Arterialization of the portal vein showed no histological, biochemical, or molecular benefits in large-for-size transplantation.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"35 6","pages":"1197-1207"},"PeriodicalIF":1.9,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39648129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute-Phase Serum Amyloid A May Predict Microvascular Invasion and Early Tumor Recurrence in Patients with Hepatitis B Virus-Related Hepatocellular Carcinoma Undergoing Liver Resection.","authors":"Xinggang Guo,&nbsp;Wenli Zhang,&nbsp;Jin Du,&nbsp;Rongsuo Tao,&nbsp;Wei Dong,&nbsp;Jian Huang,&nbsp;Jinmin Zhang,&nbsp;Zeya Pan,&nbsp;Weiping Zhou,&nbsp;Xiuli Zhu,&nbsp;Hui Liu,&nbsp;Fuchen Liu","doi":"10.1080/08941939.2022.2035858","DOIUrl":"https://doi.org/10.1080/08941939.2022.2035858","url":null,"abstract":"<p><strong>Objective: </strong>To elucidate the impact of acute-phase protein serum amyloid A (aSAA) on microvascular invasion (MVI) and early recurrence in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC).</p><p><strong>Methods: </strong>HBV-related HCC patients (<i>n</i> = 192) undergoing liver resection were included in the study. The protein levels of aSAA were analyzed by immunohistochemical staining in 172 tumor specimens, and further detected via western blotting in HCC and their corresponding portal vein tumor thrombus (PVTT) (<i>n</i> = 20). Cox and logit regression analysis was performed. Exploratory subgroup analysis was used to balance the potential confounders.</p><p><strong>Results: </strong>HBV-related HCC patients with high aSAA levels tended to have high HBV-DNA loads. Logit and Cox regression analyses revealed high expression of aSAA is an independent risk factor not only for MVI (OR 5.384, 95% CI 2.286-13.301, <i>P</i> < 0.001) but also for early recurrence (HR 6.040, 95% CI 1.970-18.540, <i>P</i> = 0.002), overall recurrence (HR 3.720, 95% CI 2.140-6.450, <i>P</i> < 0.001), and overall survival (HR 4.15, 95% CI 2.380-7.230, <i>P</i> < 0.001). Subgroup analysis showed that the effects of aSAA were consistent across all subgroups examined. Additionally, the aSAA protein level was significantly higher in PVTT than that in its corresponding tumor specimen. A high HBV-DNA level and large tumor size were the independent risk factors for early HCC recurrence in patients with high levels of aSAA.</p><p><strong>Conclusions: </strong>High expression of aSAA was an independent risk factor for MVI and early tumor recurrence in HBV-related HCC patients after liver resection. The aSAA protein level could thus be a promising biomarker for predicting MVI and early recurrence in these patients.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"35 6","pages":"1368-1376"},"PeriodicalIF":1.9,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39782988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth of Intestinal Neomucosa on Pedicled Gastric Wall Flap, a Novel Technique in an Animal Model.","authors":"Panagiotis Sakarellos,&nbsp;Apostolos Papalois,&nbsp;Harikleia Gakiopoulou,&nbsp;Iro Zacharioudaki,&nbsp;Michalis Katsimpoulas,&nbsp;Marina Belia,&nbsp;Dimitrios Moris,&nbsp;Kyveli Aggelou,&nbsp;Ilias Vagios,&nbsp;Spiridon Davakis,&nbsp;Michail Vailas,&nbsp;Theodoros Liakakos,&nbsp;Theodoros Diamantis,&nbsp;Evangelos Felekouras,&nbsp;Michael Kontos","doi":"10.1080/08941939.2022.2034196","DOIUrl":"https://doi.org/10.1080/08941939.2022.2034196","url":null,"abstract":"<p><p><b>Background:</b> Short bowel syndrome (SBS) remains an unsolved issue in modern medicine. Numerous experimental surgical techniques have been proposed in the attempt to increase the intestinal absorptive capacity.<b>Materials and Methods:</b> Ten female Landrace pigs, divided in two groups of 5 (A and B), were explored through a midline incision. A spindle-shaped vascularized full-thickness gastric wall flap (GWF) consisting of part of the major curvature with the gastroepiploic arch preserved was de-epithelialized and then placed as a \"patch\" to cover an antimesenteric border defect of either a nonfunctional blind intestinal loop (group A) or a functional intestinal loop of the gastrointestinal tract (group B). A spindle-shaped curved, rigid, low density polyethylene (LDPE) splint was sutured on the external surface of the patch in order to prevent shrinkage of GWF and collapse of the intestinal wall in group A.<b>Results:</b> There was a decrease of both dimensions of the patch. Microscopically a thin layer of columnar epithelial cells covered the center of the patch, evolving in shorter, blunt, poorly developed villi with increasing maturation laterally. The patch surface was covered by nearly 90%. In the three animals that died prematurely the coverage of GWF was negligent or suboptimal directly dependent on the length of survival.<b>Conclusions:</b> The hereby-described patching technique demonstrated the growth of intestinal neomucosa on the GWF. The capability of the stomach to provide large flaps and the advantages of the use of native tissues render this animal model valuable for the future research in the field.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"35 6","pages":"1329-1339"},"PeriodicalIF":1.9,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39947252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eye-Tracking Indicators of Workload in Surgery: A Systematic Review.","authors":"Otto Tolvanen,&nbsp;Antti-Pekka Elomaa,&nbsp;Matti Itkonen,&nbsp;Hana Vrzakova,&nbsp;Roman Bednarik,&nbsp;Antti Huotarinen","doi":"10.1080/08941939.2021.2025282","DOIUrl":"https://doi.org/10.1080/08941939.2021.2025282","url":null,"abstract":"<p><p>BackgroundEye tracking is a powerful tool for unobtrusive and real time assessment of workload in clinical settings. Before the complex eye tracking derived surrogates can be proactively utilized to improve surgical safety, the indications, validity and reliability requires careful evaluation.MethodsWe conducted a systematic review of literature from 2010 to 2020 according to PRISMA guidelines. A search on PubMed, Cochrane, Scopus, Web of science, PsycInfo and Google scholar databases was conducted on July 2020. The following search query was used\" (\"eye tracking\" OR \"gaze tracking\") AND (surgery OR surgical OR operative OR intraoperative) AND (workload OR stress)\". Short papers, no peer reviewed or papers in which eye-tracking methodology was not used to investigate workload or stress factors in surgery, were omitted.ResultsA total of 17 (N = 17) studies were identified eligible to this review. Most of the studies (<i>n</i> = 15) measured workload in simulated setting. Task difficulty and expertise were the most studied factors. Studies consistently showed surgeon's eye movements such as pupil responses, gaze patterns, blinks were associated with the level of perceived workload. However, differences between measurements in operational room and simulated environments have been found.ConclusionPupil responses, blink rate and gaze indices are valid indicators of workload. However, the effect of distractions and non-technical factors on workload is underrepresented aspect in the literature even though recognized as underlying factors in successful surgery.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"35 6","pages":"1340-1349"},"PeriodicalIF":1.9,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39689951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of Preoperative Serum and Intraoperative Peritoneal Lavage Fluid Ca-125 Levels with Postoperative Tumor Histology in Patients with Endometrial Cancer: A Prospective-Controlled Study.","authors":"Suleyman Gunhan Doluoglu,&nbsp;Mehmet Karaca,&nbsp;Onur Erol","doi":"10.1080/08941939.2021.2024307","DOIUrl":"https://doi.org/10.1080/08941939.2021.2024307","url":null,"abstract":"<p><strong>Background: </strong>The role of Ca-125 in endometrial cancer is not fully known. Some authors have reported high Ca-125 levels in patients with recurrent or advanced endometrial cancer, whereas others have stated that Ca-125 levels and the advance of the disease were not correlated in endometrial cancer. This makes it inevitable for clinicians to search for different measurement methods or interpretation of the present tumor markers. The aim of this study was to evaluate the relationship between Ca-125 values of the serum and abdominal lavage fluid and postoperative histopathological parameters in patients with endometrial carcinoma.</p><p><strong>Methods: </strong>The study included patients who were diagnosed with endometrial cancer in the Gynecology Clinic and were planned to undergo surgery. The correlations of clinicopathological parameters with preoperative values of Ca-125 measured from serum and abdominal lavage fluid were investigated. The Spearman correlation test was applied in the analysis of correlations of serum and abdominal lavage fluid Ca-125 values with postoperative tumor characteristics.</p><p><strong>Results: </strong>The serum Ca-125 values were determined to be positively correlated with surgical stage, tumor diameter, and lymph node involvement (p = 0.03; p = 0.04; and p = 0.01, respectively). No correlation was determined between tumor grade and serum Ca-125 level. The level of Ca-125 in the abdominal lavage fluid was observed to be correlated with surgical stage and tumor grade, but not with tumor diameter or lymph node involvement (p = 0.01, p = 0.04, respectively).</p><p><strong>Conclusions: </strong>The value of Ca-125 in the abdominal lavage fluid has a positive correlation with the surgical stage and tumor grade in patients with endometrial carcinoma.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"35 6","pages":"1248-1252"},"PeriodicalIF":1.9,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39790421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accurate Neurosurgery for the Establishment of the Electric Kindling Model of Epilepsy in Mice.","authors":"Verónica Custodio,&nbsp;Jorge Acosta,&nbsp;Carmen Rubio,&nbsp;Leonardo Hernández,&nbsp;Javier Brito,&nbsp;Elisa Taddei","doi":"10.1080/08941939.2022.2032488","DOIUrl":"https://doi.org/10.1080/08941939.2022.2032488","url":null,"abstract":"<p><p>This article describes in detail the essential stereotaxic neurosurgery to develop the electric experimental kindling model in mice. To date, available literature describing the methodology of the kindling model is very poor and usually neglects many relevant details about the neurosurgery, such as the manufacture of the electrodes, accurate stereotaxic coordinates of the amygdala nuclei, and the general surgery procedures (e.g., anesthesia, postsurgical recovery, fit survival of the animal's). The electric kindling model produces a progressive development of generalized tonic-clonic seizures, which can be assessed by electroencephalography and behavioral responses. The seizures displayed are produced by a repeated low-intensity electrical stimulation in specific regions of the brain that is achieved through the previous implantation of electrodes. In this study, the aim was to implant the electrodes in basolateral amygdaloid nucleus (BLA). In order to successfully establish the kindling experimental model, neurosurgery to place the electrodes is an essential step to develop the epileptogenic phenomenon. It crucial that the surgery is carried out with exceptional exactitude, because in that way the experimental model represents an accurate and valid tool to study and understand epilepsy and the results obtained can be used to develop further strategies in epilepsy clinical research.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"35 6","pages":"1253-1262"},"PeriodicalIF":1.9,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39870845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between Primary Tumor Resection for Metastatic Small Intestine Neuroendocrine Tumors and Survival: A Propensity Score-Matched Analysis.","authors":"Haihao Yan,&nbsp;Linlin Yin,&nbsp;Hao Han,&nbsp;Ye Jin,&nbsp;Zheng Liu","doi":"10.1080/08941939.2021.2024306","DOIUrl":"https://doi.org/10.1080/08941939.2021.2024306","url":null,"abstract":"<p><strong>Background: </strong>At present, it has been controversial whether primary tumor resection (PTR) can bring survival advantage to patients with metastatic small intestine neuroendocrine tumors (SI-NETs). To answer this question, we conducted a retrospective cohort study to evaluate the effect of PTR on the survival of patients with metastatic SI-NETs.</p><p><strong>Methods: </strong>Information on SI-NETs patients from 2004 to 2015 was extracted from Surveillance, Epidemiology, and End Results (SEER) databases. Demographics, tumor characteristics, treatment, and survival were compared. Propensity score-matched (PSM) was used 1:1 in the filtered queue. Cox proportional hazard regression model was used to evaluate the correlation between PTR and treatment results.</p><p><strong>Results: </strong>Before PSM, survival analysis showed that PTR significantly prolonged the survival of metastatic SI-NETs patients. After PSM, there was no significant difference in overall survival (OS) and cancer-specific survival (CSS) between the PTR group and the non-PTR group. Multivariate analysis showed no significant difference in OS and CSS between the two groups (p > 0.05).</p><p><strong>Conclusion: </strong>Our study shows that OS and CSS are comparable between the PTR group and the non-PTR group. Thus, we believe that PTR should not be actively performed on such patients. Meanwhile, it is undeniable that properly selected patients may also benefit from PTR. Therefore, prospective randomized controlled trials are still needed to verify the effect of PTR on patients in the future.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"35 6","pages":"1239-1247"},"PeriodicalIF":1.9,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39790425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RIP1 Regulates Mitochondrial Fission during Skeletal Muscle Ischemia Reperfusion Injury.","authors":"Yu Cao,&nbsp;Shunli Chen,&nbsp;Xiangqing Xiong,&nbsp;Lina Lin,&nbsp;Wantie Wang,&nbsp;Liangrong Wang","doi":"10.1080/08941939.2022.2036880","DOIUrl":"10.1080/08941939.2022.2036880","url":null,"abstract":"<p><strong>Background: </strong>Dynamin related protein-1 (Drp1)-mediated mitochondrial fission relates to ischemia reperfusion (IR) injury, and its association with necroptosis is implied. We hypothesized that receptor-interacting protein 1 (RIP1), a key kinase in necroptosis, acted as an upstream of Drp1-mediated mitochondrial fission during skeletal muscle IR.</p><p><strong>Methods: </strong>Thirty rats were randomized into the SM, IR, NI, MI, and DI group (<i>n</i> = 6). The rats in the SM group were shamly operated, and those in the IR group were subjected to 4-hour ischemia of the right hindlimb that was followed by 4-hour reperfusion. Intraperitoneal administration of Nec-1 1 mg/kg, Mdivi-1 1.2 mg/kg and same volume of DMSO were given before ischemia in the NI, MI and DI groups, respectively. Upon reperfusion, the soleus muscles were harvested to determine morphological changes and the expression of RIP1, total Drp1 and p-Drp1 (Ser616). Moreover, the muscular oxidative stress indicators and plasma muscle damage biomarkers were detected.</p><p><strong>Results: </strong>IR led to impaired histopathological structures and mitochondrial fragmentation in the soleus muscle tissue, accompanied with increased muscular oxidative stress and muscle injury biomarkers, which could be similarly alleviated by Mdivi-1 and Nec-1 (<i>p</i> < 0.05). RIP1 and p-Drp1 (Ser616) protein levels were significantly upregulated in the soleus muscle subjected to IR injury, this upregulation was attenuated in the NI group, and Mdivi-1 downregulated the protein expression of p-Drp1 (Ser616) but not of RIP1 (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>RIP1 functions as an upstream of Drp1-mediated mitochondrial fission in the execution of necroptosis during skeletal muscle IR.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"35 1","pages":"1269-1274"},"PeriodicalIF":1.9,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46386104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical, Histological, and Profibrotic Extracellular Matrix Protein Changes in a Model of Tracheal Stenosis Induced by Cervical Tracheal Autotransplantation","authors":"M. Silva-Martínez, J. R. Olmos-Zúñiga, J. Calyeca, M. Baltazares-Lipp, M. Gaxiola-Gaxiola, Andrea Nachón-Acosta, L. E. Pensado-Piedra, F. Juárez-Hernández, R. Sotelo-Robledo, R. Jasso-Victoria, Antonia Luna-Flores, J. C. Vázquez-Minero","doi":"10.1080/08941939.2022.2081388","DOIUrl":"https://doi.org/10.1080/08941939.2022.2081388","url":null,"abstract":"Abstract Background Tracheal stenosis (TS) is a complication of prolonged intubation, tracheotomy, and tracheal surgery that compromises the vascular supply. Animal models are essential for studying its pathophysiology and the effect of interventions. Objective To establish a TS model in rats secondary to tracheal autotransplantation with a graft submerged in bleomycin (Atx-Bleo). Additionally, to evaluate the clinical and histological changes, as well as the expression of newly formed collagen (NFC), isoforms of transforming growth factor beta (TGFβ), fibronectin (FN), elastin (ELN), integrin β1 (ITGβ1), and matrix metalloproteinase 1 (MMP1) in TS. Methods Twenty Wistar rats were divided into three groups: group I (n = 20) control; group II (n = 10) end-to-end anastomosis of the trachea (tracheoplasty); and group III (n = 10) Atx-Bleo. The animals were evaluated clinically, tomographically, macroscopically, morphometrically, and microscopically. NFC deposition, and the expression of profibrotic and antifibrotic proteins were evaluated in tracheal scars. Results All animals survived the surgical procedure and the study period. Compared with the other study groups, the Atx-Bleo group developed TS and fibrosis, exhibited higher expression of NFC, TGFβ1, TGFβ2, FN, ELN, and ITGβ1, and mild expression of TGFβ3 and MMP1 (p < 0.005; analysis of variance, Dunnett and Tukey tests). Conclusion Atx-Bleo in TS model rats produces tomographic and histological changes, and induces the upregulation of profibrotic proteins (TGFβ1, TGFβ2, collagen, FN, ELN, ITGβ1) and downregulation of antifibrotic proteins (TGFβ3, MMP1). Therefore, this model may be used to test new pharmacological treatments for reversing or preventing TS, and conduct basic studies regarding its pathophysiology.","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"35 1","pages":"1551 - 1561"},"PeriodicalIF":1.9,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41368627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}