Journal of Immunotoxicology最新文献_第2页

Correction. 修正。

IF 2.4 4区医学

Journal of Immunotoxicology Pub Date : 2025-12-01 Epub Date: 2025-04-11 DOI: 10.1080/1547691X.2025.2483130

引用次数: 0

Inflammatory markers in prostate cancer: potential roles in risk stratification and immune profiling. 前列腺癌的炎症标志物：在风险分层和免疫分析中的潜在作用。

IF 2.4 4区医学

Journal of Immunotoxicology Pub Date : 2025-12-01 Epub Date: 2025-04-28 DOI: 10.1080/1547691X.2025.2497776

Edgaras Burzinskis, Ieva Janulaityte, Mindaugas Jievaltas, Darijus Skaudickas, Guoda Burzinskiene, Edvinas Dainius, Albinas Naudziunas, Astra Vitkauskiene

{"title":"Inflammatory markers in prostate cancer: potential roles in risk stratification and immune profiling.","authors":"Edgaras Burzinskis, Ieva Janulaityte, Mindaugas Jievaltas, Darijus Skaudickas, Guoda Burzinskiene, Edvinas Dainius, Albinas Naudziunas, Astra Vitkauskiene","doi":"10.1080/1547691X.2025.2497776","DOIUrl":"https://doi.org/10.1080/1547691X.2025.2497776","url":null,"abstract":"Inflammation plays a critical role in prostate cancer (PCa) pathophysiology, yet the diagnostic value of specific inflammatory markers remains unclear. This study evaluates the association between circulating and tissue inflammatory markers with PCa presence and their potential as biomarkers for risk stratification. This prospective study analyzed serum and prostate biopsy samples from 60 patients with PCa and 22 cancer-free controls. Concentrations of inflammatory markers, including IL-2, IL-4, IL-10, IL-13, IL-33, Oncostatin M, TNFα, PDGF-BB, and TREM-1, were measured using Luminex technology. Statistical analyses included the Mann-Whitney test, logistic regression, and ROC curve analysis to assess differences and diagnostic performance. PCa patients exhibited significantly higher serum levels of IL-2 (p = 0.001), IL-10 (p < 0.001), IL-33 (p < 0.001), Oncostatin M (p = 0.018), and TNFα (p = 0.017) compared to controls. In contrast, biopsy tissue levels of IL-4 (p < 0.001), IL-10 (p < 0.001), IL-13 (p = 0.004), Oncostatin M (p = 0.012), PDGF-BB (p = 0.039), and TREM-1 (p = 0.013) were significantly lower in PCa patients, suggesting an inverse association. IL-10 (inverse) and IL-4 (inverse) in biopsy tissue showed high specificity in ROC analysis (AUC = 0.788 and 0.804, respectively), while IL-2 and IL-33 in serum were positively associated with PCa risk. This study suggests that IL-4, IL-10, and IL-13 in biopsy tissue may serve as biomarkers of a protective effect, while elevated IL-2 and IL-33 in serum are associated with an increased risk of PCa. These findings highlight the potential of inflammatory markers in PCa risk stratification, warranting further investigation in larger cohorts.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"22 1","pages":"2497776"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of cynomolgus monkey capsid-specific positive control cells for IFNγ ELISpot assays for adeno-associated gene therapy applications. 产生食蟹猴衣壳特异性阳性对照细胞用于IFNγ ELISpot检测，用于腺相关基因治疗应用。

IF 2.4 4区医学

Journal of Immunotoxicology Pub Date : 2025-12-01 Epub Date: 2025-02-13 DOI: 10.1080/1547691X.2025.2459931

Sandra Casinghino, Karrie Tartaro, Jessica Anderson, Ravindra C Kodihalli, Sophia G Lee, Jessie Qian, Patricia A Schneider, Richard Virgen-Slane, Laurence O Whiteley, Thomas A Lanz

{"title":"Generation of cynomolgus monkey capsid-specific positive control cells for IFNγ ELISpot assays for adeno-associated gene therapy applications.","authors":"Sandra Casinghino, Karrie Tartaro, Jessica Anderson, Ravindra C Kodihalli, Sophia G Lee, Jessie Qian, Patricia A Schneider, Richard Virgen-Slane, Laurence O Whiteley, Thomas A Lanz","doi":"10.1080/1547691X.2025.2459931","DOIUrl":"10.1080/1547691X.2025.2459931","url":null,"abstract":"Cell-mediated immune (CMI) responses to adeno-associated virus (AAV) can lead to tissue damage and loss of therapeutic transgene expression. Identifying robust biomarkers and mechanisms of CMI can aid clinical practice and advancement of AAV gene therapies. The present work evaluated peripheral blood mononuclear cells (PBMC) from non-human primates (NHP) before and after immunization with adenovirus 5 encoding AAV9 capsid antigen. PBMC were stimulated ex vivo with AAV9 capsid peptides to evaluate CMI responses by interferon (IFN)-γ ELISpot, intracellular cytokines/activation markers, secreted cytokines, and RNAseq. AAV peptide stimulation produced a robust IFNγ ELISpot 11 days after immunization and ≈ 4 years after cryopreservation. Flow cytometry revealed increased IFNγ, interleukin (IL)-2, or tumor necrosis factor (TNF)-positive T-cells. Increases in secreted CXCR3 ligands (IP-10, I-TAC) were detected. Robust changes and correlations to ELISpot responses were revealed by RNAseq, including IFNγ, IP-10, and I-TAC, many downstream transcripts, and several IFN-independent pathways. These data from AAV-immunized NHP identify biomarkers that could serve as robust and sensitive supplements/alternatives to ELISpot for early detection of CMI responses. Assessment of these biomarkers in non-clinical and clinical studies is a critical next step to determine the translation of this work to administration of a therapeutic AAV vector.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"22 1","pages":"2459931"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143408652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soy allergy in patients with atopic dermatitis, sensitivity of ALEX2 Allergy Xplorer examination. 大豆过敏患者的特应性皮炎，敏感性ALEX2过敏症探索者检查。

IF 3.1 4区医学

Journal of Immunotoxicology Pub Date : 2025-12-01 Epub Date: 2025-09-11 DOI: 10.1080/1547691X.2025.2549686

Jarmila Čelakovská, Eva Čermáková, Ctirad Andrýs, Moeina Afshari, Jan Krejsek

{"title":"Soy allergy in patients with atopic dermatitis, sensitivity of ALEX2 Allergy Xplorer examination.","authors":"Jarmila Čelakovská, Eva Čermáková, Ctirad Andrýs, Moeina Afshari, Jan Krejsek","doi":"10.1080/1547691X.2025.2549686","DOIUrl":"https://doi.org/10.1080/1547691X.2025.2549686","url":null,"abstract":"The aim of the current study was to evaluate the incidence of soy allergy in patients with atopic dermatitis (AD) and to evaluate the results of specific IgE against molecular components of soy. Altogether, 100 AD patients were examined. Soy allergy was confirmed in an open exposure test (history), and the presence of specific IgE against molecular components of soy (Gly m 4, Gly m 5, Gly m 6, Gly m 8) was evaluated using an ALEX2 Allergy Explorer test. The results for the measures of specific IgE against molecular components of soy (Gly m 4, Gly m 5, Gly m 6, Gly m 8) and clinical reactions in the open exposure test were then compared. Soy allergy was confirmed in 12% of patients. The sensitivity of specific IgE against Gly m 4 was 50.0% (21.1-78.9%). In another 29% of patients we recorded the positive results for specific IgE against Gly m 4 without any clinical reaction to soy. Compared to results from a previous study in 2013, there was an increase in the incidence of soy allergy in AD patients. An elimination diet and an exposure test are recommended to detect a reaction to soy. ALEX2 Allergy Xplorer test gives us a comprehensive picture of sensitization and the possibility of evaluation of cross-reacting allergens.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"22 1","pages":"2549686"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between CD200 molecule expression on B lymphocytes and levels of specific IgE against molecular components of storage mites, animals and molds in atopic dermatitis patients. 特应性皮炎患者B淋巴细胞CD200分子表达与抗储螨、动物和霉菌分子成分特异性IgE水平的关系

IF 3.1 4区医学

Journal of Immunotoxicology Pub Date : 2025-12-01 Epub Date: 2025-10-01 DOI: 10.1080/1547691X.2025.2561586

Jarmila Čelakovská, Eva Čermáková, Petra Boudková, Marie Jandová, Lenka Čáková, Ctirad Andrýs, Moeina Afshari, Jan Krejsek

{"title":"Association between CD200 molecule expression on B lymphocytes and levels of specific IgE against molecular components of storage mites, animals and molds in atopic dermatitis patients.","authors":"Jarmila Čelakovská, Eva Čermáková, Petra Boudková, Marie Jandová, Lenka Čáková, Ctirad Andrýs, Moeina Afshari, Jan Krejsek","doi":"10.1080/1547691X.2025.2561586","DOIUrl":"https://doi.org/10.1080/1547691X.2025.2561586","url":null,"abstract":"This study investigated the relationship between CD200 molecule expression on B- lymphocytes and the levels of specific IgE to molecular components of storage mites (Gly d 2, Lep d 2), dog (Can f 1, Can f 2), cat (Fel d 1), shrimp (Pen m 2), molds (Asp f 6, Mala s 11, Alt a 6, Alt a 1, Mala s 6, Cla h), and German cockroach (Bla g 9) in patients with atopic dermatitis (AD), both with and without dupilumab therapy. The study included 46 patients with AD- 26 without dupilumab treatment and 20 with dupilumab treatment. Serum levels of specific IgE were measured using the ALEX2 Allergy Xplorer diagnostic microarray and CD200 molecule was evaluated with the use of flow cytometry. Spearman's rank correlation coefficient was used to assess the relationship between B-lymphocyte CD200 expression and specific IgE levels to molecular components. According to the results, the association between CD200 expression and specific IgE levels to molecular components was low (up to 10%) in AD patients without dupilumab therapy. In patients with dupilumab therapy, the association was non-linear, indicating that the two monitored parameters had opposite effects. In conclusion, the present study did not confirm any association between the CD200 molecule on B-lymphocytes and specific IgE levels to molecular components.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"22 1","pages":"2561586"},"PeriodicalIF":3.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and semi-quantification of protein allergens in complex mixtures using proteomic and AllerCatPro 2.0 bioinformatic analyses: a proof-of-concept investigation. 利用蛋白质组学和 AllerCatPro 2.0 生物信息学分析鉴定和半定量化复杂混合物中的蛋白质过敏原：概念验证研究。

IF 2.4 4区医学

Journal of Immunotoxicology Pub Date : 2024-12-01 Epub Date: 2024-01-31 DOI: 10.1080/1547691X.2024.2305452

Nora L Krutz, Ian Kimber, Jason Winget, Minh N Nguyen, Vachiranee Limviphuvadh, Sebastian Maurer-Stroh, Catherine Mahony, G Frank Gerberick

{"title":"Identification and semi-quantification of protein allergens in complex mixtures using proteomic and AllerCatPro 2.0 bioinformatic analyses: a proof-of-concept investigation.","authors":"Nora L Krutz, Ian Kimber, Jason Winget, Minh N Nguyen, Vachiranee Limviphuvadh, Sebastian Maurer-Stroh, Catherine Mahony, G Frank Gerberick","doi":"10.1080/1547691X.2024.2305452","DOIUrl":"10.1080/1547691X.2024.2305452","url":null,"abstract":"The demand for botanicals and natural substances in consumer products has increased in recent years. These substances usually contain proteins and these, in turn, can pose a risk for immunoglobulin E (IgE)-mediated sensitization and allergy. However, no method has yet been accepted or validated for assessment of potential allergenic hazards in such materials. In the studies here, a dual proteomic-bioinformatic approach is proposed to evaluate holistically allergenic hazards in complex mixtures of plants, insects, or animal proteins. Twelve commercial preparations of source materials (plant products, dust mite extract, and preparations of animal dander) known to contain allergenic proteins were analyzed by label-free proteomic analyses to identify and semi-quantify proteins. These were then evaluated by bioinformatics using AllerCatPro 2.0 (https://allercatpro.bii.a-star.edu.sg/) to predict no, weak, or strong evidence for allergenicity and similarity to source-specific allergens. In total, 4,586 protein sequences were identified in the 12 source materials combined. Of these, 1,665 sequences were predicted with weak or strong evidence for allergenic potential. This first-tier approach provided top-level information about the occurrence and abundance of proteins and potential allergens. With regards to source-specific allergens, 129 allergens were identified. The sum of the relative abundance of these allergens ranged from 0.8% (lamb's quarters) to 63% (olive pollen). It is proposed here that this dual proteomic-bioinformatic approach has the potential to provide detailed information on the presence and relative abundance of allergens, and can play an important role in identifying potential allergenic hazards in complex protein mixtures for the purposes of safety assessments.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"21 1","pages":"2305452"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139642283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lung-delivered IL-10 therapy elicits beneficial effects via immune modulation in organic dust exposure-induced lung inflammation. 肺输送IL-10疗法通过免疫调节对有机粉尘暴露诱发的肺部炎症产生有益影响。

IF 2.4 4区医学

Journal of Immunotoxicology Pub Date : 2024-12-01 Epub Date: 2024-04-02 DOI: 10.1080/1547691X.2024.2332172

Aaron D Schwab, Todd A Wyatt, Amy J Nelson, Angela Gleason, Rohit Gaurav, Debra J Romberger, Jill A Poole

{"title":"Lung-delivered IL-10 therapy elicits beneficial effects via immune modulation in organic dust exposure-induced lung inflammation.","authors":"Aaron D Schwab, Todd A Wyatt, Amy J Nelson, Angela Gleason, Rohit Gaurav, Debra J Romberger, Jill A Poole","doi":"10.1080/1547691X.2024.2332172","DOIUrl":"10.1080/1547691X.2024.2332172","url":null,"abstract":"Efficacious therapeutic options capable of resolving inflammatory lung disease associated with environmental and occupational exposures are lacking. This study sought to determine the preclinical therapeutic potential of lung-delivered recombinant interleukin (IL)-10 therapy following acute organic dust exposure in mice. Here, C57BL/6J mice were intratracheally instilled with swine confinement organic dust extract (ODE) (12.5%, 25%, 50% concentrations) with IL-10 (1 μg) treatment or vehicle control intratracheally-administered three times: 5 hr post-exposure and then daily for 2 days. The results showed that IL-10 treatment reduced ODE (25%)-induced weight loss by 66% and 46% at Day 1 and Day 2 post-exposure, respectively. IL-10 treatment reduced ODE (25%, 50%)-induced lung levels of TNFα (-76%, -83% [reduction], respectively), neutrophil chemoattractant CXCL1 (-51%, -60%), and lavage fluid IL-6 (-84%, -89%). IL-10 treatment reduced ODE (25%, 50%)-induced lung neutrophils (-49%, -70%) and recruited CD11cintCD11b+ monocyte-macrophages (-49%, -70%). IL-10 therapy reduced ODE-associated expression of antigen presentation (MHC Class II, CD80, CD86) and inflammatory (Ly6C) markers and increased anti-inflammatory CD206 expression on CD11cintCD11b+ cells. ODE (12.5%, 25%)-induced lung pathology was also reduced with IL-10 therapy. In conclusion, the studies here showed that short-term, lung-delivered IL-10 treatment induced a beneficial response in reducing inflammatory consequences (that were also associated with striking reduction in recruited monocyte-macrophages) following acute complex organic dust exposure.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"21 1","pages":"2332172"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11137733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation into changes in inflammatory and immune cell markers in pre-diabetic patients from Durban, South Africa. 调查南非德班糖尿病前期患者炎症和免疫细胞标志物的变化。

IF 3.3 4区医学

Journal of Immunotoxicology Pub Date : 2024-12-01 Epub Date: 2023-12-15 DOI: 10.1080/1547691X.2023.2290282

Nomusa Christina Mzimela, Aubrey Mbulelo Sosibo, Phikelelani Siphosethu Ngubane, Andile Khathi

{"title":"Investigation into changes in inflammatory and immune cell markers in pre-diabetic patients from Durban, South Africa.","authors":"Nomusa Christina Mzimela, Aubrey Mbulelo Sosibo, Phikelelani Siphosethu Ngubane, Andile Khathi","doi":"10.1080/1547691X.2023.2290282","DOIUrl":"10.1080/1547691X.2023.2290282","url":null,"abstract":"The prevalence of pre-diabetes is increasing in rapidly urbanizing cities, especially in individuals aged 25 - 45 years old. Studies also indicate that this condition is associated with aberrant immune responses that are also influenced by environmental factors. This study sought to investigate changes in the concentration of immune cells and select inflammatory markers in patients with pre-diabetes in Durban, South Africa. Blood samples collected from King Edward Hospital, after obtaining ethics approval, were divided into non-diabetic (ND), pre-diabetic (PD) and type 2 diabetic (T2D) using ADA criteria. In each sample, the concentration of immune cells and select inflammatory markers were determined. The results showed a significant increase in eosinophil and basophil levels in the PD group as compared to the ND group. Compared to ND, the PD and T2D groups had significant increases in serum TNFα, CD40L and fibrinogen concentrations. Additionally, there were decreases in serum CRP, IL-6, and P-selectin in the PD group while these markers increased in the T2D group. These findings were indicative of immune activation and highlight the impact of pre-diabetes in this population. More studies are recommended with a higher number of samples that are stratified by gender and represent the gender ratio in the city.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"21 1","pages":"2290282"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138806241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HLA-A02 restricted T-cell cross-reactivity to a microbial antigen. HLA-A02 限制性 T 细胞与微生物抗原的交叉反应。

IF 2.4 4区医学

Journal of Immunotoxicology Pub Date : 2024-12-01 Epub Date: 2024-07-27 DOI: 10.1080/1547691X.2024.2373247

Alar Aints, Marina Šunina, Raivo Uibo

{"title":"HLA-A02 restricted T-cell cross-reactivity to a microbial antigen.","authors":"Alar Aints, Marina Šunina, Raivo Uibo","doi":"10.1080/1547691X.2024.2373247","DOIUrl":"10.1080/1547691X.2024.2373247","url":null,"abstract":"Molecular mimicry has been proposed to be a possible mechanism of induction of autoimmunity. In some cases, it is believed that such events could lead to a disease such as Type 1 diabetes (T1D). One of the primary MHC-I epitopes in the non-obese diabetic (NOD) mouse model of T1D has been identified as a peptide from the islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP) protein. In humans, the most common MHC-I model allele is HLA-A02; based on this, the study here identified a potential HLA-A0201-restricted human IGRP epitope as YLKTNLFLFL and also found a homologous A0201-restricted peptide in an Enterococcal protein. Using cells obtained from healthy human donors, it was seen that after a 2-week incubation with the synthetic bacterial protein, healthy A0201+ donor CD8+ cells displayed increased staining for human IGRP-peptide-dextramer. On the other hand, in control cultures, no significant levels of dextramer-staining CD8+ T-cells were detectable. From these outcomes, it is possible to conclude that certain bacterial proteins may initiate CD8+ T-cell-mediated immune reaction toward homologous human antigens.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"21 1","pages":"2373247"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of dermal sensitization by nickel salts in a novel humanized TLR-4 mouse model. 镍盐在新型人源TLR-4小鼠模型中的皮肤致敏性评估。

IF 2.4 4区医学

Journal of Immunotoxicology Pub Date : 2024-12-01 Epub Date: 2024-12-04 DOI: 10.1080/1547691X.2024.2414979

K A Roach, S E Anderson, C Waggy, J Aldinger, A B Stefaniak, J R Roberts

{"title":"Assessment of dermal sensitization by nickel salts in a novel humanized TLR-4 mouse model.","authors":"K A Roach, S E Anderson, C Waggy, J Aldinger, A B Stefaniak, J R Roberts","doi":"10.1080/1547691X.2024.2414979","DOIUrl":"10.1080/1547691X.2024.2414979","url":null,"abstract":"The fundamental goal of this study was to determine the potential utility of a novel humanized Toll-like receptor-4 (hTLR-4) mouse model for future in vivo studies of nickel allergy. First, mice of both sexes and hTLR-4 expression profiles were incorporated into a Local Lymph Node Assay (LLNA) to assess skin sensitization. Next, a set of hTLR-4 hTLR-4-positive mice (female and male groups) was similarly exposed to vehicle control (VC) or 10% NiSO4 on Days 1, 2, and 3. Mice were euthanized on Day 10, lymph node (LN) cellularity was assessed, LN and spleen cells were phenotyped, and serum was collected to quantify circulating cytokine and IgE levels. In the LLNA, hTLR-4-positive mice of both sexes exhibited enhanced responsivity to nickel. NiSO4 (10%) had a stimulation index (SI) of 3.7 (females) and 3.8 (males) in hTLR-4-positive animals, and an SI of 0.5 (females) and 0.8 (males) in hTLR-4 hTLR-4-negative mice. In the 10d study, hTLR-4-positive mice exposed to 10% NiSO4 exhibited increased LN cellularity (6.0× increase in females, 3.2× in males) and significantly higher concentrations of circulating IgE (4.1× increase in females, 3.4× in males). Significant increases in serum interferon (IFN)-γ, interleukin (IL)-4, and IL-5 levels were seen in female mice, while altered concentrations of IL-4 and IL-10 were detected in male mice. The results of this study ultimately demonstrate that murine expression of hTLR-4 confers enhanced susceptibility to dermal sensitization by nickel, and consequently, the hTLR-4 mouse model represents a viable approach for future studies of nickel allergy in vivo.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"21 1","pages":"2414979"},"PeriodicalIF":2.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0