Journal of Immunotoxicology最新文献

In Memorial: Kenneth L. Hastings, DrPH, DABT, FATS. 纪念：肯尼斯·l·黑斯廷斯，博士，学士学位，脂肪。

IF 3.1 4区医学

Journal of Immunotoxicology Pub Date : 2026-12-01 Epub Date: 2026-03-03 DOI: 10.1080/1547691X.2026.2639297

Robert V House, Marc J Pallardy

引用次数: 0

Molecular sensitization patterns in atopic dermatitis: linking food and airborne allergens using ISAC. 特应性皮炎的分子致敏模式：使用ISAC连接食物和空气中的过敏原。

IF 3.1 4区医学

Journal of Immunotoxicology Pub Date : 2026-12-01 Epub Date: 2026-06-11 DOI: 10.1080/1547691X.2026.2677913

Čelakovská J, Bukač J, Krejsek J, Andrýs C

{"title":"Molecular sensitization patterns in atopic dermatitis: linking food and airborne allergens using ISAC.","authors":"Čelakovská J, Bukač J, Krejsek J, Andrýs C","doi":"10.1080/1547691X.2026.2677913","DOIUrl":"10.1080/1547691X.2026.2677913","url":null,"abstract":"Objective: The aim of our study is to evaluate the laboratory results of sensitization to molecular components in atopic dermatitis patients and to show if there is the significant relation between the sensitization to molecular components of food allergens (regardless of the occurrence of clinical reactions) and the sensitization to molecular components of inhalant allergens.Materials and methods: The complete dermatological and allergological examination including the examination with Multiplex ISAC testing was performed in all included patients. This study analyzed the data to determine the relation between the sensitization to molecular components of food and inhalant allergens. This study used the statistical calculation with matrix.Results, discussion, and conclusion: Altogether 100 atopic dermatitis patients were examined - 48 men, 52 women, the average age 40.9 years. The significant relation between the sensitization to molecular components of timothy and molecular components of food allergens (PR 10 proteins, storage proteins, and lipid transfer proteins) was confirmed. Also, the significant relation between the sensitization to PR 10 proteins of food allergens and sensitization to Aln g 1, Bet v 1 (PR 10 proteins), Ole e 9 (Beta-1,3-glucanase), and Bet v 2, Mer a 1 (Profilin) was recorded.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"23 1","pages":"2677913"},"PeriodicalIF":3.1,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148226066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prolonged brain cytokine elevation and microgliosis following exposure to World Trade Center dust particulate matter in male spontaneously hypertensive rats. 暴露于世贸中心尘埃颗粒物质后，雄性自发性高血压大鼠脑细胞因子升高和小胶质细胞增生。

IF 2.5 4区医学

Journal of Immunotoxicology Pub Date : 2026-12-01 Epub Date: 2026-08-30 DOI: 10.1080/1547691X.2026.2722654

Kailey M Jerome, Brandon M Gibson, Matthew W Gorr, Neill A Schwieterman, Mitchell D Cohen, Loren E Wold, Colin K Combs

{"title":"Prolonged brain cytokine elevation and microgliosis following exposure to World Trade Center dust particulate matter in male spontaneously hypertensive rats.","authors":"Kailey M Jerome, Brandon M Gibson, Matthew W Gorr, Neill A Schwieterman, Mitchell D Cohen, Loren E Wold, Colin K Combs","doi":"10.1080/1547691X.2026.2722654","DOIUrl":"10.1080/1547691X.2026.2722654","url":null,"abstract":"Exposure to World Trade Center (WTC) dust following the September 11, 2001 attacks has been linked to numerous negative health outcomes in First Responders, including cognitive impairment disproportionately impacting First Responders with aging. However, the mechanisms underlying neurocognitive decline remain poorly characterized. This investigation examined the effects of WTC dust exposure on myelin integrity and neuroinflammatory signaling in twelve-week old male spontaneously hypertensive rats. To model Ground Zero exposure in First Responders, WTC dust was intratracheally administered for two consecutive days (2.43 [± 0.78] mg/day). It was hypothesized that WTC dust exposure would enhance neuroinflammatory signaling and promote an earlier, progressive decline of myelin abundance. Rats were euthanized at 3 and 12 mo post-exposure. Changes in myelin abundance (Black-Gold II), myelin basic protein (MBP) and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), and microglial responses (ionized calcium-binding protein; Iba1) were assessed through immunohistochemical staining, and cytokines were measured in brain homogenates. WTC dust exposure resulted in increased Black-Gold II staining at 3 mo despite no changes in MBP or CNPase. In contrast, microglial activation and IL-1β concentrations were elevated in dust-exposed rats at 12 mo. Collectively, these data suggest WTC dust exposure induces sustained neuroinflammatory signaling and may elicit a compensatory myelin response indicative of early neurodegeneration.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"23 1","pages":"2722654"},"PeriodicalIF":2.5,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13652033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Imazalil at high plasma concentrations: implications for neutrophil immunophenotype. 高血浆浓度的伊马扎利：对中性粒细胞免疫表型的影响。

IF 2.5 4区医学

Journal of Immunotoxicology Pub Date : 2026-12-01 Epub Date: 2026-05-21 DOI: 10.1080/1547691X.2026.2672428

Wioletta Ratajczak-Wrona, Malgorzata Rusak, Olga Diana Drezek, Joanna Chociej-Stypulkowska, Agnieszka Iwaniuk, Katarzyna Bielawska, Jolanta Wrobel, Agnieszka Zebrowska, Marzena Garley, Luiza Czaczkowska, Milena Dabrowska, Wojciech Miltyk, Piotr Radziwon, Slawomir Wolczynski, Dariusz Skarzynski, Barbara Pucelik, Ewa Jablonska

{"title":"Imazalil at high plasma concentrations: implications for neutrophil immunophenotype.","authors":"Wioletta Ratajczak-Wrona, Malgorzata Rusak, Olga Diana Drezek, Joanna Chociej-Stypulkowska, Agnieszka Iwaniuk, Katarzyna Bielawska, Jolanta Wrobel, Agnieszka Zebrowska, Marzena Garley, Luiza Czaczkowska, Milena Dabrowska, Wojciech Miltyk, Piotr Radziwon, Slawomir Wolczynski, Dariusz Skarzynski, Barbara Pucelik, Ewa Jablonska","doi":"10.1080/1547691X.2026.2672428","DOIUrl":"10.1080/1547691X.2026.2672428","url":null,"abstract":"Neutrophil functions are tightly regulated by numerous membrane receptors, the activation of which determines the appropriate response to various endogenous and exogenous factors. Accumulating evidence indicates that environmental endocrine-disrupting chemicals (EDC) can modulate immune system function, inter alia, by influencing neutrophil activity. Imazalil (IMZ), an EDC known to exhibit antagonistic activity against the androgen receptor expressed on neutrophils, exemplifies a compound that may potentially affect these processes. The aim of this study was to evaluate the effect of imazalil and testosterone on the expression of CD molecules associated with fundamental neutrophil functions. Whole blood collected from healthy men served as the study material. Plasma imazalil concentrations were determined using GC-MS. Isolated neutrophils were incubated in the presence of IMZ (at a concentration established in the present study: 28 ng/ml) or testosterone (at a physiological concentration). Cytometric analysis revealed that exposing cells to imazalil resulted in an elevated percentage of neutrophils expressing CD14, CD66b, CD62L, and CD284, accompanied by a decrease in the percentage of cells expressing CD10, CD11c, CD15, CD49d HLA-DR, as well as a reduction in MPO+ neutrophils. No changes in the percentage of neutrophils expressing the analyzed molecules were observed in the presence of testosterone. In conclusion, imazalil modulates the neutrophil immunophenotype, which may inhibit functions such as adhesion, migration, chemotaxis, and phagocytosis. Consequently, this may predispose individuals exposed to this compound to innate immunity disorders.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"23 1","pages":"2672428"},"PeriodicalIF":2.5,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147987916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the immunotoxicity of atrazine and its chloro-metabolites: relevance for human cancer. 阿特拉津及其氯代谢物的免疫毒性评价：与人类癌症的相关性。

IF 2.5 4区医学

Journal of Immunotoxicology Pub Date : 2026-12-01 Epub Date: 2026-01-21 DOI: 10.1080/1547691X.2025.2604501

Charles Breckenridge, James W Simpkins, Ralph Cooper

{"title":"Evaluation of the immunotoxicity of atrazine and its chloro-metabolites: relevance for human cancer.","authors":"Charles Breckenridge, James W Simpkins, Ralph Cooper","doi":"10.1080/1547691X.2025.2604501","DOIUrl":"10.1080/1547691X.2025.2604501","url":null,"abstract":"This review was undertaken because it is known that high doses of atrazine activate the HPA axis, leading to increased corticosterone secretion, which could result in immunosuppression. The mammalian immunotoxicity of atrazine was evaluated based on in vitro and in vivo studies and the association between chlorotriazine use and the risk of cancer in humans based on epidemiological studies. In vitro studies reported that μM concentrations of atrazine may adversely affect cytokine production, lymphocytes, and dendritic cell function. However, there were no consistent patterns of effects of the chlorotriazines on the developing or mature immune system in sub-chronic, chronic, and lifetime animal studies. In a detailed immunotoxicity study on atrazine in rats, there were no effects of atrazine treatment on T-cell-dependent, IgM antibody production or natural killer cell activity. Based on the compendium of toxicology data reviewed, it was concluded that atrazine is unlikely to be immunotoxic at any dose to which humans might realistically be exposed. This conclusion was supported by epidemiological studies indicating that there was no consistent association between occupational exposure to atrazine and cancers of the immune system.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"23 1","pages":"2604501"},"PeriodicalIF":2.5,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating miRNAs as predictive biomarkers for drug-induced autoimmune toxicity in D-penicillamine induced autoimmune diseases animal model. 评价mirna作为d -青霉胺诱导的自身免疫性疾病动物模型中药物诱导的自身免疫性毒性的预测性生物标志物。

IF 2.5 4区医学

Journal of Immunotoxicology Pub Date : 2026-12-01 Epub Date: 2026-04-20 DOI: 10.1080/1547691X.2026.2622342

Ren Yuke, Qu Zhe, Zhang Di, Yang Yanwei, Huo Guitao, Li Shuangxing, Jiang Hua, Li Lulu, Lin Zhi

{"title":"Evaluating miRNAs as predictive biomarkers for drug-induced autoimmune toxicity in D-penicillamine induced autoimmune diseases animal model.","authors":"Ren Yuke, Qu Zhe, Zhang Di, Yang Yanwei, Huo Guitao, Li Shuangxing, Jiang Hua, Li Lulu, Lin Zhi","doi":"10.1080/1547691X.2026.2622342","DOIUrl":"10.1080/1547691X.2026.2622342","url":null,"abstract":"Drug-induced autoimmune (DIA) toxicity represents a significant challenge in drug safety evaluation, and traditional autoantibody biomarker antinuclear antibodies (ANA) exhibits limitations in DIA assessment, highlighting the urgent need for more reliable biomarkers. This study employed d-penicillamine to induce an autoimmune disease model in Brown Norway (BN) rats and systematically investigated the expression changes of autoimmunity-related microRNAs (miRNAs) in spleen, exploring their potential value as novel biomarkers for predicting DIA toxicity. A total of 30 male BN rats were randomly divided into control group (0 mg/kg), low-dose d-penicillamine group (150 mg/kg), and high-dose group (450 mg/kg), with necropsy performed at days 7 and 14 post-administration. ANA detection, histopathological examination of spleen, surface immune-related proteins CD11a and CD278 on splenic CD3+CD4+ T-cells, and miRNA expression profiling in spleen tissue were conducted. Receiver operating characteristic (ROC) curves were plotted to evaluate the correlation of ANA and miRNAs with the degree of splenic pathological damage, and the relationships between miRNAs and both ANA and CD11a/CD278 were analyzed. The results demonstrated that 16 miRNAs were significantly up-regulated in spleen tissue, among which 13 miRNAs showed superior predictive performance (AUC = 0.828-0.898) compared to the traditional biomarker ANA (AUC = 0.783), with most of these miRNAs exhibiting correlation with CD11a/CD278 expression. These findings indicate that aberrant miRNA expression in the d-penicillamine-induced autoimmune disease preclinical model in BN rats holds potential for early prediction of DIA toxicity, with some miRNAs demonstrating higher diagnostic value than ANA, thereby providing novel molecular biomarker candidates for early diagnosis of DIA toxicity.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"23 1","pages":"2622342"},"PeriodicalIF":2.5,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147729198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predicting cross reactivity between the fire ant venom allergen Sol i 3 and the antigen 5 from vespid venoms based on molecular modeling computing. 基于分子模拟计算的火蚁毒液过敏原soli3与毒蛇毒液抗原5的交叉反应性预测。

IF 2.5 4区医学

Journal of Immunotoxicology Pub Date : 2026-12-01 Epub Date: 2026-06-10 DOI: 10.1080/1547691X.2026.2661829

Amilcar Perez-Riverol, Luis Gustavo Romani Fernandes, José Roberto Aparecido Dos Santos-Pinto, Alexis Musacchio Lasa, Brita Dorn, Maria Beatrice Biló, Thilo Jakob, Ricardo de Lima Zollner, Mario Sergio Palma

{"title":"Predicting cross reactivity between the fire ant venom allergen Sol i 3 and the antigen 5 from vespid venoms based on molecular modeling computing.","authors":"Amilcar Perez-Riverol, Luis Gustavo Romani Fernandes, José Roberto Aparecido Dos Santos-Pinto, Alexis Musacchio Lasa, Brita Dorn, Maria Beatrice Biló, Thilo Jakob, Ricardo de Lima Zollner, Mario Sergio Palma","doi":"10.1080/1547691X.2026.2661829","DOIUrl":"10.1080/1547691X.2026.2661829","url":null,"abstract":"The fire ant Solenopsis invicta is an aggressive invasive species whose venom frequently triggers hypersensitivity reactions, including severe anaphylaxis. In endemic regions, its stings represent a significant cause of Hymenoptera-related allergy. Four venom allergens have been identified - phospholipase A1 (Sol i 1), antigen 2 (Sol i 2), antigen 3 (Sol i 3), and antigen 4 (Sol i 4) - with Sol i 3 recognized as the predominant sensitizer. However, the molecular determinants that drive Sol i 3 allergenicity and its potential cross-reactivity with other Hymenoptera venoms remain insufficiently understood. This study identified the linear immunoglobulin E (IgE) epitopes of Sol i 3 and examined their recognition by sera from yellow jacket venom (YJV) - and Polistes wasp-allergic patients. Two linear epitopes were mapped: Sol i 3_e1 (ELRQRVASGKEMRG) and Sol i 3_e2 (WAKTTKIGCGRIMF). Although Sol i 3 exhibits limited sequence and structural similarity to other antigen 5 proteins, it contains a conserved immunoreactive core (WAKTTK), analogous to the WAKTKE motif described for the allergen Poly p 5 from Polybia paulista. This conserved region may represent a shared epitope contributing to cross-reactivity among Hymenoptera venoms. Consistently, sera from P. dominula-sensitized patients and YJV-sensitized patients recognized Sol i 3_e2. These findings define key B-cell epitopes of Sol i 3 and reveal a conserved motif that may underlie cross-reactivity, offering implications for improved diagnosis and immunotherapy.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"23 1","pages":"2661829"},"PeriodicalIF":2.5,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148211414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species differences influencing the safety assessment of CD3 T-cell engagers. 物种差异影响CD3 t细胞接合物的安全性评估。

IF 3.1 4区医学

Journal of Immunotoxicology Pub Date : 2026-12-01 Epub Date: 2026-06-13 DOI: 10.1080/1547691X.2026.2667356

Taylor L Hickman, David A Gallegos, Shihui Zhong, Lauren Gauthier, Matthew P Wagoner, Mansoor M Amiji

{"title":"Species differences influencing the safety assessment of CD3 T-cell engagers.","authors":"Taylor L Hickman, David A Gallegos, Shihui Zhong, Lauren Gauthier, Matthew P Wagoner, Mansoor M Amiji","doi":"10.1080/1547691X.2026.2667356","DOIUrl":"https://doi.org/10.1080/1547691X.2026.2667356","url":null,"abstract":"Bispecific CD3 T-cell engagers (TCE) redirect endogenous T-cells to tumor cells and have demonstrated substantial clinical activity, but their mechanism of action also drives immune-mediated toxicities that are often underpredicted in nonhuman primate (NHP) studies. To investigate the biological basis of this translational gap, we performed a comparative evaluation of human and cynomolgus macaque T-cells using immunophenotyping, functional characterization with TCE, and single-cell transcriptomic profiling. Human and NHP donors showed broadly similar T-cell frequencies, subset distributions, and robust responses to polyclonal stimulation, confirming preserved intrinsic effector capacity in both species. However, across multiple CD20-, BCMA-, and GPRC5D-targeted TCE, human PBMC consistently exhibited greater cytotoxic potency and markedly elevated IFNγ production relative to NHP. Transcriptomic analyses revealed that although global TCR signaling architecture is conserved, human T-cells exhibit increased expression of some proximal signaling mediators, including LAT, PAG1, SKP1, and PI3K-family genes. These functional and molecular differences provide a mechanistic explanation for reduced pharmacologic responsiveness observed in NHP toxicology studies. While NHP studies can provide valuable insight for identifying antigen-dependent cytotoxic hazards, their limitations underscore the increasing importance of incorporating human-relevant models in nonclinical safety assessment.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"23 1","pages":"2667356"},"PeriodicalIF":3.1,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148251883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micro- and nanoplastics-mediated immunometabolic reprogramming: a systemic nexus for chronic inflammation and multi-organ disease risk. 微和纳米塑料介导的免疫代谢重编程：慢性炎症和多器官疾病风险的系统性联系。

IF 2.5 4区医学

Journal of Immunotoxicology Pub Date : 2026-12-01 Epub Date: 2026-05-05 DOI: 10.1080/1547691X.2026.2665189

Jingxuan Dai, Kun Yuan, Keming Huang, Yingyan Sun, Xuancheng Zhou, Ying Zhang

{"title":"Micro- and nanoplastics-mediated immunometabolic reprogramming: a systemic nexus for chronic inflammation and multi-organ disease risk.","authors":"Jingxuan Dai, Kun Yuan, Keming Huang, Yingyan Sun, Xuancheng Zhou, Ying Zhang","doi":"10.1080/1547691X.2026.2665189","DOIUrl":"10.1080/1547691X.2026.2665189","url":null,"abstract":"Micro- and nanoplastics (MNP) are ubiquitous environmental stressors increasingly detected in human tissues and directly linked to clinical cardiovascular events. This review proposes immunometabolic reprogramming as the central nexus through which MNP drive systemic immune dysregulation and chronic inflammation. Upon entering via multiple routes, MNP selectively sequester in immune organs, disrupting mitochondrial quality control via Drp1-mediated fission and activating the cGAS-STING pathway. Furthermore, MNP induce a pseudohypoxic state that stabilizes HIF-1a, driving a glycolytic \"Warburg-like\" shift that promotes pro-inflammatory macrophage polarization. These intracellular perturbations are further amplified by \"lipid corona\" formation and gut microbiota dysbiosis, which depletes anti-inflammatory short-chain fatty acids. Collectively, this systemic immunometabolic remodeling provides a mechanistic framework for understanding MNP-related risks for cardiovascular, metabolic, and neurodegenerative disorders. This review emphasizes the necessity of integrating immunometabolic parameters into future environmental health risk assessment frameworks.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"23 1","pages":"2665189"},"PeriodicalIF":2.5,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-vitro exposure to mercury chloride, with and without n-3 long chain polyunsaturated fatty acids, on the Th17-associated immune response in peripheral blood mononuclear cells from systemic lupus erythematosus patients. 体外暴露于氯化汞（含或不含n-3长链多不饱和脂肪酸）对系统性红斑狼疮患者外周血单个核细胞th17相关免疫反应的影响

IF 2.5 4区医学

Journal of Immunotoxicology Pub Date : 2026-12-01 Epub Date: 2026-10-29 DOI: 10.1080/1547691X.2026.2745858

Cealan O Henry, Emeir M McSorley, David J Armstrong, J J Strain, Philip J Allsopp

{"title":"In-vitro exposure to mercury chloride, with and without n-3 long chain polyunsaturated fatty acids, on the Th17-associated immune response in peripheral blood mononuclear cells from systemic lupus erythematosus patients.","authors":"Cealan O Henry, Emeir M McSorley, David J Armstrong, J J Strain, Philip J Allsopp","doi":"10.1080/1547691X.2026.2745858","DOIUrl":"https://doi.org/10.1080/1547691X.2026.2745858","url":null,"abstract":"TH17 cytokines are implicated in systemic lupus erythematosus (SLE) pathogenesis. Mercury is an environmental factor associated with the exacerbation of SLE pathology, potentially by regulating the ThH17 pathway. Humans are primarily exposed to mercury from dental amalgams and fish consumption. Fish are also a rich source of the n-3 long chain polyunsaturated fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and have been shown to reduce SLE disease symptoms. This pilot in-vitro investigation isolated peripheral blood mononuclear cells (PBMC) from SLE patients (N = 15) and healthy controls (HC, N = 15). The cells were stimulated with lipopolysaccharide (LPS) and exposed to 200 nM of mercury (II) chloride (HgCl) for 24 hr, with or without pre-exposure (24 hr) to either EPA or DHA at 100 μM each. The TH17-associated cytokines were quantified in the PBMC supernatants. Pre-exposure to EPA resulted in significantly lower concentrations of IL-27 in supernatants of LPS-HgCl treated PBMC from SLE patients (29.58 [± 22.62] pg/mL) compared to HCs (51.63 [± 42.96] pg/mL) (p = 0.04). While DHA-LPS-HgCl had significantly lower IL-27 concentrations compared in LPS-HgCl in PBMC from SLE patients and HCs, there was no significant difference between groups. IL-27 exhibits pro/anti-inflammatory effects on the immune system. We suggest that the observed reductions in IL-27 concentrations following EPA-LPS-HgCl treatment support the protective role of n-3 PUFA in autoimmune responses.","PeriodicalId":16073,"journal":{"name":"Journal of Immunotoxicology","volume":"23 1","pages":"2745858"},"PeriodicalIF":2.5,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149287153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0