Journal of immunopharmacology最新文献_第7页

CPH-86, a highly purified podophyllotoxin, efficiently suppresses in vivo and in vitro immune responses. CPH-86是一种高纯度鬼臼毒素，能有效抑制体内和体外免疫反应。

Journal of immunopharmacology Pub Date : 1985-01-01 DOI: 10.3109/08923978509026477

C Brigati, B Sander

引用次数: 9

Miconazole influence on both cellular immune response and hepatic drug metabolism in mice. 咪康唑对小鼠细胞免疫反应和肝脏药物代谢的影响。

Journal of immunopharmacology Pub Date : 1985-01-01 DOI: 10.3109/08923978509047631

J Descotes, P André, R Tedone, J C Evreux

引用次数: 1

The effect of methotrexate on in vitro immunoglobulin production. 甲氨蝶呤对体外免疫球蛋白生成的影响。

Journal of immunopharmacology Pub Date : 1985-01-01 DOI: 10.3109/08923978509047636

A M O'Meara, J Brazil, D J Reen

引用次数: 10

A large scale method of separating multiple lymphokines secreted by the murine EL-4 thymoma. 分离小鼠EL-4胸腺瘤分泌的多种淋巴因子的大规模方法。

Journal of immunopharmacology Pub Date : 1985-01-01 DOI: 10.3109/08923978509026466

A E Chang, M T Lotze, R S Ames, J A Roth, S A Rosenberg

{"title":"A large scale method of separating multiple lymphokines secreted by the murine EL-4 thymoma.","authors":"A E Chang, M T Lotze, R S Ames, J A Roth, S A Rosenberg","doi":"10.3109/08923978509026466","DOIUrl":"https://doi.org/10.3109/08923978509026466","url":null,"abstract":"The in vivo administration of lymphokines is a new approach to immunotherapy with possible applications to the treatment of tumors and infectious processes. These lymphokines are produced in very small quantities and purification of these molecules has proven difficult. A rapid two step method was used to isolate different lymphokines from large quantities of conditioned media produced by the murine EL-4 cell line. Interleukin-2 (IL-2), colony stimulating factor (CSF) and macrophage activating factor (MAF) are lymphokines with distinct biologic activities and are secreted by the EL-4 cell line. Their isolation involved initial batch purification on trimethylsilyl-controlled pore glass beads followed by reversed phase high pressure liquid chromatography. Recovery of IL-2 activity was greater than 100% of initial activity. The specific activity of the purified IL-2 ranged from 2 X 10(6) to 3.6 X 10(7) U/mg protein. Colony stimulating factor (CSF) and macrophage activating factor (MAF) were also separated on the chromatographic columns. This technique is useful for the purification of large quantities of these lymphokines which have potential in vivo applications.","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"7 1","pages":"17-31"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08923978509026466","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15007487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effects of hydrazyl group containing drugs on leucocyte functions: an immunoregulatory model for the hydralazine-induced lupus-like syndrome. 含肼类药物对白细胞功能的影响:肼致狼疮样综合征的免疫调节模型。

Journal of immunopharmacology Pub Date : 1985-01-01 DOI: 10.3109/08923978509026483

R E Schopf, H M Hanauske-Abel, G Tschank, H Schulte-Wissermann, V Günzler

{"title":"Effects of hydrazyl group containing drugs on leucocyte functions: an immunoregulatory model for the hydralazine-induced lupus-like syndrome.","authors":"R E Schopf, H M Hanauske-Abel, G Tschank, H Schulte-Wissermann, V Günzler","doi":"10.3109/08923978509026483","DOIUrl":"https://doi.org/10.3109/08923978509026483","url":null,"abstract":"Isoniazid (INH) and hydralazine (HYD) are transglutaminase (TGase, E.C.2.3.2.13.) substrates containing catalytically recruitable hydrazyl groups. Since they can be expected to inhibit TGase-mediated cell functions by competing with physiological substrates, their effect upon allogeneically and lectin-induced proliferation of mononucleocytes and upon zymosan-induced chemiluminescence of phagocytes was studied. Both compounds inhibited chemiluminescence in a dose-dependent manner. ID50 of HYD was consistently below 20 microM, while that of INH was above 120 microM. Proliferation of immunocompetent cells was suppressed by HYD with an ID50 of 60 microM, INH was inhibitory only above 5000 microM. Analogs of both compounds not containing hydrazyl groups proved to be inactive. Control experiments indicated that inhibition is not due to toxicity or lipophilicity of the compounds, structural analogs lacking a hydrazyl moiety were inactive. It is suggested that, in vivo, HYD interferes with signal-induced TGase-dependent leucocyte functions essential for immunologic stability, and that the resultant dysregulation with disruption of self tolerance contributes to the HYD promoted lupus-like syndrome.","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"7 4","pages":"385-401"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08923978509026483","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14004679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

The effect of delta-9-tetrahydrocannabinol and 11-hydroxy-delta-9-tetrahydrocannabinol on T-lymphocyte and B-lymphocyte mitogen responses. δ -9-四氢大麻酚和11-羟基δ -9-四氢大麻酚对t淋巴细胞和b淋巴细胞丝裂原反应的影响。

Journal of immunopharmacology Pub Date : 1985-01-01 DOI: 10.3109/08923978509026487

T W Klein, C A Newton, R Widen, H Friedman

{"title":"The effect of delta-9-tetrahydrocannabinol and 11-hydroxy-delta-9-tetrahydrocannabinol on T-lymphocyte and B-lymphocyte mitogen responses.","authors":"T W Klein, C A Newton, R Widen, H Friedman","doi":"10.3109/08923978509026487","DOIUrl":"https://doi.org/10.3109/08923978509026487","url":null,"abstract":"Previous studies have shown that delta-9-tetrahydrocannabinol (THC) suppresses T-lymphocyte proliferation when added to human cell cultures. We report that THC when added to mouse splenocyte cultures suppressed T-lymphocyte (Con A, PHA) and B-lymphocyte (LPS) mitogen-induced proliferation. Although the ED50 concentrations (5 micrograms/ml; 1.6 X 10(-5)M) of THC were similar for suppressing all three mitogen responses, higher threshold concentrations of drug were required to effect suppression of the T-lymphocyte mitogen responses. Complete suppression of T- and B-lymphocyte responses was achieved with THC concentrations (8 micrograms/ml or 2.6 X 10(-5)M) which were not directly toxic as judged by vital dye exclusion. The hydroxylated metabolite of THC, 11-hydroxy-THC, was observed to be much less potent in the inhibition of lymphocyte proliferation. However, as with the parent compound, B-lymphocyte responses appeared to be the most affected by the drug. Additional studies demonstrated that both T- and B-lymphocyte proliferation is rapidly suppressed following THC treatment, not affected by a 24 hr. pretreatment with THC, and not as readily suppressed by THC in cultures containing 20% serum. Thus, THC appears to inhibit both T- and B-lymphocyte proliferation with B-lymphocyte responses displaying greater inhibition at lower drug concentration. The 11-hydroxy metabolite is much less suppressive in this system than the parent compound.","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"7 4","pages":"451-66"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08923978509026487","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14136666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 101

Theophylline induced non specific suppressor activity in human peripheral blood lymphocytes. 茶碱诱导人外周血淋巴细胞的非特异性抑制活性。

Journal of immunopharmacology Pub Date : 1985-01-01 DOI: 10.3109/08923978509047635

M R Zocchi, R Pardi, G Gromo, E Ferrero, M E Ferrero, C Besana, C Rugarli

{"title":"Theophylline induced non specific suppressor activity in human peripheral blood lymphocytes.","authors":"M R Zocchi, R Pardi, G Gromo, E Ferrero, M E Ferrero, C Besana, C Rugarli","doi":"10.3109/08923978509047635","DOIUrl":"https://doi.org/10.3109/08923978509047635","url":null,"abstract":"It is wellknown that theophylline yields phenotypic changes on suppressor cells. In the present study we investigated the possibility that theophylline could directly induce a suppressor activity on a lymphocyte subpopulation. We observed that a short preincubation (120 min at 37 degrees C) with theophylline (1mM) activates human peripheral blood lymphocytes to suppress mitogenic response of autologous cells. This activity was not evident on a T cell subpopulation depleted of theophylline-sensitive (T-sens) lymphocytes. Theophylline mediated suppressor activity is only present in the Concanavalin A stimulated cultures, thus suggesting a synergism between Concanavalin A and theophylline in the expression of non specific suppression. Moreover we observed that after a 24 hrs preincubation of lymphocytes in complete culture medium there was a complete loss of theophylline-induced suppression. Such a preincubation time also produced a decrease in the theophylline-mediated enhancement of intracellular 3', 5' cyclic adenosine monophosphate levels and the impairment of E-rosette formation, suggesting that theophylline acts mainly on a \"short-lived\" suppressor lymphocyte subset.","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"7 2","pages":"217-34"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08923978509047635","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14126599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

SLO4, a new interferon inducer isolated from Klebsiella pneumoniae and Escherichia coli. 从肺炎克雷伯菌和大肠埃希菌中分离的一种新的干扰素诱导剂SLO4。

Journal of immunopharmacology Pub Date : 1985-01-01 DOI: 10.3109/08923978509026468

S Stefanos, C Vanderhoven, J Wietzerbin, R Falcoff, Y Page

引用次数: 3

Modulation of lymphokine-induced macrophage activation by estrogen metabolites. 雌激素代谢物对淋巴因子诱导的巨噬细胞活化的调节。

Journal of immunopharmacology Pub Date : 1985-01-01 DOI: 10.3109/08923978509047637

R W Pfeifer, R M Patterson

{"title":"Modulation of lymphokine-induced macrophage activation by estrogen metabolites.","authors":"R W Pfeifer, R M Patterson","doi":"10.3109/08923978509047637","DOIUrl":"https://doi.org/10.3109/08923978509047637","url":null,"abstract":"Pharmacological doses of estrogens such as 17-beta estradiol (17- beta E) and diethylstilbestrol (DES) activate macrophages in a thymic-dependent manner in vivo. In this report, we investigated the direct in vitro effects of 17- beta E and its major metabolites on macrophage activation in response to lectin-stimulated lymphocyte supernatants containing macrophage-activating factor (MAF), a T cell lymphokine (LK). Activation was measured in terms of macrophage cytostasis against cultured tumor cells. As suggested by previous studies with quinone metabolites of benzene, the catechol estrogen metabolite 2-OH estrone (2-OH E) was the most potent metabolite at suppressing LK-induced macrophage activation. However, if macrophages were first LK-induced, and then exposed to estrogens before addition of tumor cells, then all the estrogens, including 2-OH E, enhanced cytostasis. These observations suggested membrane-mediated immunomodulation of macrophage function by estrogen metabolites and, indirectly, a role for the thymus in these effects via the maintenance of a mature, LK-producing T cell population necessary for macrophage activation.","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"7 2","pages":"247-63"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08923978509047637","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15011920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Ciamexone--a new highly selective immunosuppressive compound. Ciamexone——一种新的高选择性免疫抑制化合物。

Journal of immunopharmacology Pub Date : 1985-01-01 DOI: 10.3109/08923978509026474

U Bicker, W Pahlke

引用次数: 13