{"title":"甲氨蝶呤对体外免疫球蛋白生成的影响。","authors":"A M O'Meara, J Brazil, D J Reen","doi":"10.3109/08923978509047636","DOIUrl":null,"url":null,"abstract":"<p><p>The effect of Methotrexate (MTX) on pokeweed mitogen (PWM) induced immunoglobulin (Ig) production in vitro was studied over a range of 10(-4) - 10(-12)M MTX, using an enzyme-linked-immunosorbent assay (ELISA). MTX, at a concentration of 5 X 10(-9)M profoundly decreased all classes of Ig production, IgM greater than IgG greater than IgA. Plasma cell numbers, identified using polyclonal antihuman immunoglobulin, demonstrated a similar sensitivity to MTX. There was a paradoxical increase in IgM and IgG production at 5 X 10(-11)M MTX. Clinical implications of these findings are discussed.</p>","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"7 2","pages":"235-45"},"PeriodicalIF":0.0000,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08923978509047636","citationCount":"10","resultStr":"{\"title\":\"The effect of methotrexate on in vitro immunoglobulin production.\",\"authors\":\"A M O'Meara, J Brazil, D J Reen\",\"doi\":\"10.3109/08923978509047636\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The effect of Methotrexate (MTX) on pokeweed mitogen (PWM) induced immunoglobulin (Ig) production in vitro was studied over a range of 10(-4) - 10(-12)M MTX, using an enzyme-linked-immunosorbent assay (ELISA). MTX, at a concentration of 5 X 10(-9)M profoundly decreased all classes of Ig production, IgM greater than IgG greater than IgA. Plasma cell numbers, identified using polyclonal antihuman immunoglobulin, demonstrated a similar sensitivity to MTX. There was a paradoxical increase in IgM and IgG production at 5 X 10(-11)M MTX. Clinical implications of these findings are discussed.</p>\",\"PeriodicalId\":16049,\"journal\":{\"name\":\"Journal of immunopharmacology\",\"volume\":\"7 2\",\"pages\":\"235-45\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1985-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3109/08923978509047636\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of immunopharmacology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/08923978509047636\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of immunopharmacology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/08923978509047636","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The effect of methotrexate on in vitro immunoglobulin production.
The effect of Methotrexate (MTX) on pokeweed mitogen (PWM) induced immunoglobulin (Ig) production in vitro was studied over a range of 10(-4) - 10(-12)M MTX, using an enzyme-linked-immunosorbent assay (ELISA). MTX, at a concentration of 5 X 10(-9)M profoundly decreased all classes of Ig production, IgM greater than IgG greater than IgA. Plasma cell numbers, identified using polyclonal antihuman immunoglobulin, demonstrated a similar sensitivity to MTX. There was a paradoxical increase in IgM and IgG production at 5 X 10(-11)M MTX. Clinical implications of these findings are discussed.
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