{"title":"Value Chain and Business Development Strategy of MOCAF Cap Kujang in Sumedang Regency","authors":"M. I. Pribadi, W. Gunawan, R. Maulani","doi":"10.5614/3bio.2022.4.2.1","DOIUrl":"https://doi.org/10.5614/3bio.2022.4.2.1","url":null,"abstract":"MOCAF Cap Kujang is one of the MOCAF (Modified Cassava Flour) produced in Sumedang that has been produced since 2012, but until now, the business development is still limited. The challenges in MOCAF Cap Kujang are the limited availability of raw materials, the lack of intensive marketing, and the government has not paid attention to the MOCAF business in Sumedang. This study aims to analyze value chain activities in MOCAF Cap Kujang, identify internal and external factors that influence the MOCAF Cap Kujang business, and determine the business development strategy for the MOCAF Cap Kujang business. The method used for value chain analysis was Porter's model and Hayami Method. Internal factor analysis was carried out using IFE, and external factors using EFE. The results of IFE and EFE were used to formulate strategies on the SWOT matrix. The resulting strategy was then prioritized using the AHP method. The study's results showed that value chain activities in the MOCAF Cap Kujang business consist of primary activities and supporting activities that generate added value at MOCAF by Rp1000/kg (33.33%). The main strength is the clean white of MOCAF Cap Kujang, and the main weakness is that MOCAF Cap Kujang's price is higher than other MOCAFs. The main opportunity of MOCAF Cap Kujang is that MOCAF can be a substitute for wheat for people who cannot consume gluten and the main threat is the lower price of wheat flour. MOCAF Cap Kujang's business development strategy prioritizes cooperation with various parties.","PeriodicalId":160459,"journal":{"name":"3BIO: Journal of Biological Science, Technology and Management","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126665814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Setiawan Setiawan, Annisa Nadhilah, Rita P Ilhamsari
{"title":"Effects of Leaf Storage and Distillation Time on the Quality of Eucalyptus (Eucalyptus grandis) Essential Oil","authors":"Setiawan Setiawan, Annisa Nadhilah, Rita P Ilhamsari","doi":"10.5614/3bio.2022.4.1.1","DOIUrl":"https://doi.org/10.5614/3bio.2022.4.1.1","url":null,"abstract":"Essential oils such as eucalyptus oil are widely used as antioxidants, antimicrobial, anti-inflammatory, fragrance, and other medical needs. This oil is extracted from eucalyptus terpenoid compounds, e.g., cineole. This study aims to evaluate the effect of the leaf storage time and distillation time (DT) on Eucalyptus grandis essential oil yield and quality. Three DT treatments (i.e., 2 h, 3 h, and 4 h) and storage time of eucalyptus leaf in warehouse (i.e., 2 h, 3 h, and 4 h) were applied on eucalyptus leaf samples. The quality of the eucalyptus essential oil was determined using cineole content, solubility in alcohol, and optical rotation. The essential oil yield ranges between 0.165-0.220%, with the maximum yield of 0.220% on 3 days of storage and 4-hour distillation time. The cineole content ranged between 10-30% and the highest at 3 h DT with a storage time of 2 days. The optical rotation on the tested specimen showed that the index of refraction produced in oil projections was positive. The samples in all treatments showed high solubility in alcohol. This study showed that storage time of 3 days and 3-hour distillation time resulted in the most optimal eucalyptus essential oil yield, and the excellent quality of oil produced has a high potential for health product applications.","PeriodicalId":160459,"journal":{"name":"3BIO: Journal of Biological Science, Technology and Management","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124780947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Heterologous Production of Human Papillomavirus L1 Capsid Protein: Systematic Review and Meta-analysis","authors":"Andre Hendrawan, A. Fibriani","doi":"10.5614/3bio.2022.4.1.5","DOIUrl":"https://doi.org/10.5614/3bio.2022.4.1.5","url":null,"abstract":"The coverage of HPV vaccination in Indonesia remains low due to the high-cost vaccination. The vaccine prices were affected by the production rate of L1, the active substance of HPV vaccines. L1 has been produced using various organisms with varying L1 production rates and immunogenicity. A systematic review and meta-analysis were conducted to determine the organism producing L1 with the highest production, treatments affecting the L1 expression rate, and immunogenicity (represented by anti-L1 IgG titer in mice). The data of L1 titer, induction period, and IgG titer were extracted from 19 articles that have passed the articles screening. The L1 titer and induction period data were used to calculate the L1 production rate, while the IgG titer was used in the immunogenicity analysis. On a 95% confidence level, the meta-analysis revealed weak evidence that E. coli produced L1 at the highest rate. The highest IgG titer was induced using L1 expressed in Saccharomyces cerevisiae, albeit insufficient evidence on 95% confidence level. Pearson’s correlation analysis showed that the concentration of glucose, IPTG, NH4+, K+, Ca2+, Mn2+, Fe2+, Zn2+, B4O72−, H2PO4−, HPO42−, Mo7O246−, and citric acid had a positive correlation with L1 production rate in E. coli. The treatment injection doses positively correlated with IgG titer in S. cerevisiae. This study reveals the mineral salts as the potential treatments to increase L1 production rates.","PeriodicalId":160459,"journal":{"name":"3BIO: Journal of Biological Science, Technology and Management","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128003370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Fadhlullah, S. B. Prasetyati, Imam Pudoli, Calvin C. Lo
{"title":"Preliminary Economic Potential Evaluation of Seaweed Gracilaria sp. Biomass Waste as Bioindustry Feedstock Through a Biorefinery Approach: A Case Study in Karawang, Indonesia","authors":"M. Fadhlullah, S. B. Prasetyati, Imam Pudoli, Calvin C. Lo","doi":"10.5614/3bio.2022.4.1.6","DOIUrl":"https://doi.org/10.5614/3bio.2022.4.1.6","url":null,"abstract":"Seaweed processing usually produces biomass waste from unused and rejected materials, which have economic potential due to their bioactive components. This preliminary study aims to evaluate the economic potential of seaweed biomass waste through a biorefinery approach. Seaweed Gracilaria sp. biomass waste samples were collected from a representative seaweed production unit in Karawang, Indonesia, and their biochemical composition was analyzed. Relevant information related to seaweed biomass waste was gathered. The preliminary economic evaluation was assessed from the gross revenue of the proposed products, based on the assumed annual seaweed biomass waste productivity, biochemical composition, and estimated market price of the products. The present study revealed that the dry weight of Gracilaria sp. biomass waste contains 63.2% carbohydrates, 13.6% proteins, 1.6% lipids, and 21.5% ashes. Heavy metals were detected in the biomass waste, although no pigments were detected. This study estimates that 52 metric tons/year of Gracilaria sp. biomass waste could generate amino acids, fatty acids, lipids, carbohydrates, and minerals with potential gross revenue of ± USD 222,924.6/year. This study suggests that Gracilaria sp. biomass waste could be potentially used as feedstock to improve its economic value for bioindustry purposes. ","PeriodicalId":160459,"journal":{"name":"3BIO: Journal of Biological Science, Technology and Management","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124664505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Widjaksana, E. Lestari, M. Maskur, Sudarsih Sudarsih
{"title":"Autosterilization Effect of Gamma Radiation in Non-sterile Radiopharmaceuticals","authors":"W. Widjaksana, E. Lestari, M. Maskur, Sudarsih Sudarsih","doi":"10.5614/3bio.2022.4.1.3","DOIUrl":"https://doi.org/10.5614/3bio.2022.4.1.3","url":null,"abstract":"Gamma radiation is commonly used in sterilizing various products because of its microbial-killing property. Several radiopharmaceuticals are processed aseptically, which requires a well-managed GMP-based facility. This study aims to ensure that radiopharmaceutical can behave as an auto sterilizing agent since it contains radionuclide that emits gamma radiation. Sm-153-ethylenediaminetetramethylenephosphonate (Sm-153-EDTMP) and I-131-Hippuran were used as models in this study, in which various radioactivities of these products were added into non-sterile vials in the non-aseptic working area and tested for sterility using direct inoculation method. The result showed that samples containing 7 mCi of Sm-153-EDTMP and less than 2 mCi of I-131-orthoiodohippurate (I-131-Hippuran) changed the clarity of the media, but not for those containing higher radioactivity. The results showed that Sm-153-EDTMP and I-131-Hippuran at certain radioactivity can auto sterilize themselves, whereas the positive control sample and the products diluted with non-sterile water remained unsterile. This study showed that radiopharmaceuticals have auto sterilizing properties at relatively low radioactivity, depending on the products’ bioburden. Therefore, the usual aseptic preparation of some radiopharmaceuticals can be considered terminal sterilization.","PeriodicalId":160459,"journal":{"name":"3BIO: Journal of Biological Science, Technology and Management","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123773303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Estimation of Minimum Viable Population of Long-tailed Macaques (Macaca fascicularis Raffles 1821) in Support of Wildlife Management in Ir. H. Djuanda Grand Forest Park","authors":"Hatta Vrazila, E. Sumarga, H. Ramdan","doi":"10.5614/3bio.2022.4.1.2","DOIUrl":"https://doi.org/10.5614/3bio.2022.4.1.2","url":null,"abstract":"Most long-tailed macaques (LTMs) in Southeast Asia are commensal animals, which means they can coexist and benefit from humans. This interaction potentially triggers disturbance and disease transmission caused by LTMs, for instance, in Ir. Djuanda Grand Forest Park (GFP). A previous study indicates an overpopulation of LTMs in this area. The need for population control of this macaque requires appropriate ecological considerations, including determining the minimum viable population (MVP). This study aims to estimate the MVP of LTMs in Ir. Djuanda GFP area is the key input for its population control scheme. The MVP is determined based on population growth simulation using the Lefkovitch matrix. In combination with the estimate of population growth, the MVP was used to formulate the population control scheme of LTMs, by considering the current wildlife management regulations and the perception of Ir. Djuanda GFP area manager obtained through interviews. This study found six groups of LTMs in Ir. Djuanda GFP with a total population of 229 individuals, a sex ratio of 1:1.05, an age class ratio of infants, juveniles, sub-adults, and adults of 16.6%:25.7%:32,3%:25.3%, and a population growth rate of 19.7%/year. The estimate of MVP of this primate is 230 individuals, with an average group size of 38 individuals and an age class ratio of infants, juveniles, sub-adults, and adults 30%:9.1%:27.4%:23.5%. Based on wildlife management regulations and area manager perceptions, population control schemes can be implemented for relocation and captive purposes. The population control scheme consists of an age-class control scheme and a group control scheme. The age-class control scheme controls the population by 334 individuals, while the colony control scheme controls 406 individuals.","PeriodicalId":160459,"journal":{"name":"3BIO: Journal of Biological Science, Technology and Management","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132023529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Myrea Chalil, K. Meitha, R. E. Putra, Fathia Aulia Rahmah, Ridho R Sinatra, Anindha Ajeng Putri Winanta
{"title":"Wheatgrass microgreen with high antioxidants content in an urban indoor farming system","authors":"Myrea Chalil, K. Meitha, R. E. Putra, Fathia Aulia Rahmah, Ridho R Sinatra, Anindha Ajeng Putri Winanta","doi":"10.5614/3bio.2022.4.1.4","DOIUrl":"https://doi.org/10.5614/3bio.2022.4.1.4","url":null,"abstract":"Urban lifestyle is identical to stressful life and sedentary habit, leading to the increase of chronic conditions such as diabetes and cardiovascular-related diseases. Antioxidants are renowned for maintaining cellular function by quenching radicals produced in stressful conditions or infection. Fresh fruits and vegetables are the primary sources of antioxidants, but the long postharvest and transport system may reduce the benefits for the urban population. Hence, we designed a cultivation method to produce wheatgrass microgreens with high antioxidants in an urban indoor farming system. Generally, plants require light at the wavelength of 663 and 642 nm (red) and 430 nm and 453 nm (blue) to allow photosynthesis and production of secondary metabolites, such as antioxidants. We applied the LED lights with an RGB ratio of 91R/9B, 83R/17B, 47R/53B, 35R/65B, and white florescent as the control. Our results showed that 91R/9B reduced fresh mass and chlorophyll content, which might be due to the suppression of photosynthesis capacity. Interestingly, we found a significant (p<0.05) increase in carotenoids and flavonoid contents due to light combinations of 35R/65B and 83R/17B, respectively. However, the total antioxidants capacity was similar among all treatments. Carotenoids and flavonoids are among the antioxidants with a significant role in decreasing the risks of chronic diseases and their potential as antiviral agents. This cultivation system of wheat microgreen could be a promising solution to routinely supply carotenoids and flavonoids to the urban population. Further, it is also considered more environmentally friendly as it could be performed in a limited amount of land (vertically) and potentially use less energy for distribution.","PeriodicalId":160459,"journal":{"name":"3BIO: Journal of Biological Science, Technology and Management","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131662829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Suci Awaliyah, S. N. Widiyanto, R. Maulani, A. Hidayat, Ujang Dinar Husyari, T. S. Syamsudin, Erly Marwani
{"title":"Correlation of Microclimate of West Java on Caffeine and Chlorogenic acid in Coffea canephora var. robusta","authors":"Suci Awaliyah, S. N. Widiyanto, R. Maulani, A. Hidayat, Ujang Dinar Husyari, T. S. Syamsudin, Erly Marwani","doi":"10.5614/3bio.2022.4.1.7","DOIUrl":"https://doi.org/10.5614/3bio.2022.4.1.7","url":null,"abstract":"Caffeine and chlorogenic acid (CGA) are two compounds that play a role in determining the quality of coffee. The amount of the two compounds may vary depending on the environment where they are grown. This study aimed to determine the correlation between the local microclimatic condition and the concentration of caffeine and CGA in green and roasted beans of Robusta coffee from six different cultivation areas in West Java, Indonesia (i.e., Ciamis, Tasikmalaya, Sumedang, Kuningan, Cianjur, and Bogor). Samples of green beans and roasted beans were extracted with 70% methanol for caffeine analysis and ethyl acetate for CGA analysis. Caffeine and CGA were analyzed by UV-HPLC using a C18 shimpack gist shimadzu column, with an isocratic elution of methanol:water (1:1) at a 1 mL/min flow rate. Detection was performed at λ272 nm and λ324 nm for caffeine and chlorogenic acid, respectively. Principal component analysis (PCA) was used to evaluate the correlation between microclimate with caffeine and chlorogenic acid. Results indicated that the concentration of caffeine ranged from 7.67 to 16.52% and 10.79 to 15.56% in the green and roasted bean coffee, respectively. The concentration of CGA ranged from 0.74 to 3.03% and 0.25 to 0.77% in the green and roasted bean coffee, respectively. Based on PCA analysis, the most influential microclimate on the caffeine concentration were the humidity, temperature, and altitude, with the total variance of PC1 and PC2 of 76.3%. However, there was no positive correlation between the measured microclimate and the CGA concentration. In conclusion, Robusta coffee's caffeine content is positively affected by the microclimatic condition (i.e., humidity, temperature, and altitude).","PeriodicalId":160459,"journal":{"name":"3BIO: Journal of Biological Science, Technology and Management","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127394019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Review on The Production of Fermented Beverage as a Post-Harvest Processing Alternative For Mango, Banana and Purple Sweet Potato","authors":"S. Yurista, P. Aditiawati","doi":"10.5614/3bio.2021.3.2.3","DOIUrl":"https://doi.org/10.5614/3bio.2021.3.2.3","url":null,"abstract":"West Java is one of the regions in Indonesia that produces large numbers of mango, banana, and purple sweet potato. After harvesting, these commodities will undergo physical, chemical, and physiological changes so that further post-harvest processing is needed. One of the post-harvest processing that can be done is fermentation. Fermenting mango, banana, and purple sweet potato into wine is a simple and efficient method that can increase the economic value of the product. Wine is an alcoholic beverage made from grapes; however, any fruit and tuber could be used for wine-making. The article reviews the potential of mango, banana, and purple sweet potato for wine production, the microbes involved, and pretreatments of mango, banana, and purple sweet potato.","PeriodicalId":160459,"journal":{"name":"3BIO: Journal of Biological Science, Technology and Management","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124319467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andriwibowo Andriwibowo, A. Basukriadi, Erwin Nurdin
{"title":"Spatial Distributions and Model Selections of Commercial Estuarine Fish (Sciaenidae) Populations Related to Water Quality, Chl-a, and AML in Musi River mouth, South Sumatra","authors":"Andriwibowo Andriwibowo, A. Basukriadi, Erwin Nurdin","doi":"10.5614/3bio.2021.3.2.1","DOIUrl":"https://doi.org/10.5614/3bio.2021.3.2.1","url":null,"abstract":"Estuary and river mouth are essential habitats for many commercial estuarine fishes, including the Sciaenidae family. While recently, estuaries have been threatened by anthropogenic marine litter (AML) transported from nearby land and river. An important type of AML is plastic litter since it takes a long degradation time. In the South Sumatra Province, Indonesia, one of the vital estuaries is the Musi estuary. This paper aims to map the spatial distributions of two Sciaenids, including Panna microdon and Otolithoides pama, and Sciaenid’s environmental covariates, including water quality, chlorophyll a, and plastic litters in Musi estuary and model the correlations of Sciaenids with their covariates. The maps were developed using GIS, and the model was validated using AIC methods. The data were collected from 3 river mouths in the west, central, and east of the Musi estuary. The data showed that the populations of both Sciaenids were higher in the east river mouth rather than in the west. Sciaenid populations were positively correlated with high salinity, DO, chlorophyll a, moderate transparency, and low temperature. A high load of AML’s frequency (7.54 items/m2) and weights (36.8 gram/m2) has reduced both Sciaenid populations in the central river mouth of the estuary. In contrast, low AML loads in the east have correlated with high Sciaenid populations. Model selection based on AIC values shows the best model for P.microdon retained an effect of AML weight with AIC values of 22.591 and 28.321 for O. pama. This concludes that the weight of plastic litter in estuary water was the main limiting factor for Sciaenid populations in Musi.","PeriodicalId":160459,"journal":{"name":"3BIO: Journal of Biological Science, Technology and Management","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132485214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}