Journal of Hard Tissue Biology最新文献

{"title":"Glucose Affects the Quality and Properties of Hard Tissue in Diabetes Mellitus Model","authors":"Qingchao Wu, I. Yamawaki, Y. Taguchi, Kei Shiomi, Daisuke Kimura, Tsurayuki Takahashi, M. Umeda","doi":"10.2485/jhtb.31.29","DOIUrl":"https://doi.org/10.2485/jhtb.31.29","url":null,"abstract":"Patients with diabetes mellitus (DM) are at an increased risk of tooth loss compared to healthy individuals. Although studies human subjects suggest that diabetes control is affected by periodontitis, there is scarce mechanistic evidence supporting its biological plausibility. Therefore, using type 2 DM rat bone marrow mesenchymal stromal cells (BMMSCs) were incubated with four concentrations of glucose (5.5, 8.0, 12.0 or 24.0 mM), the effects of different glucose concentrations on BMMSCs stemness and osteogenesis were evaluated. High glucose concentrations decreased the fluorescence intensity of β-actin, STRO-1, CD73 and CD90. Moreover, cell proliferation decreased at high glucose concentrations. Alkaline phosphatase activity was decreased at 12.0 mM and 24.0 mM. In contrast, osteocalcin production and calcium deposition were considerably increased at 24.0 mM. Differences in the calcium/phosphate ratio associated with various glucose concentrations were similar to calcium deposition. The mRNA expression of Runx2 and inflammatory cytokines increased with increasing glucose concentration. The RANKL/OPG ratio decreased at high glucose concentrations. A high glucose concentration increased hard tissue formation, but the quality and stemness of the mineralized tissue decreased. Thus, hard tissue had a high risk of bone resorption in the case of uncontrolled diabetes even if periodontal treatment stabilized state of periodontitis for a moment.","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"lncRNA ZNF710-AS1 Acts as a ceRNA for miR-146a-5p and miR-146b-5p to Accelerate Osteogenic Differentiation of PDLSCs by Upregulating the BMP6/Smad1/5/9 Pathway","authors":"Ying Liu, D. Fu","doi":"10.2485/jhtb.31.231","DOIUrl":"https://doi.org/10.2485/jhtb.31.231","url":null,"abstract":": Multiple experimental pieces of evidence have confirmed that fully understanding the regulatory mechanisms of osteogenic differentiation of periodontal ligament stem cells (PDLSCs) can better promote and improve the ability of perio dontal tissues to regenerate and alleviate periodontal diseases. This study aimed to reveal whether the long noncoding RNA (lncRNA) ZNF710-AS1 plays a role in the osteogenic differentiation of PDLSCs and its molecular mechanisms. Microarray datasets GSE159507 and GSE159508 were retrieved from the Gene Expression Omnibus database and differentially ex pressed genes were identified using R language (limma package). The results revealed that the expression of ZNF710-AS1 and bone morphogenetic protein 6 (BMP6) was upregulated whereas that of miR-146a-5p/miR-146b-5p was downregulated during the osteogenic differentiation of PDLSCs. PDLSCs were successfully isolated and cultured in vitro . Osteogenic and adipogenic differentiation abilities were evaluated by performing alizarin red staining and oil red O staining, respectively. Overexpression of ZNF710-AS1 significantly increased the osteogenic differentiation ability of PDLSCs by upregulating the expression of BMP6 and phosphorylation-SMAD family member 1/5/9 (p-Smad1/5/9) and competitively sponging miR-146a-5p/miR-146b-5p and acting as a competing endogenous RNA (ceRNA). This study demonstrated that ZNF710-AS1 promotes the osteogenic differentiation of PDLSCs by upregulating BMP6/Smad1/5/9 expression and acting as a ceR NA for miR-146a-5p and miR-146b-5p.","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"92 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Anti-Inflammatory Effect of 6% HES 200/0.5 on RAW264.7 Cells Induced by LPS through HMGB1/NF-κB Signaling Pathway","authors":"J Zhang, Yongli Wang, Jianzhong Zhang, Shaoyan Huang","doi":"10.2485/jhtb.31.245","DOIUrl":"https://doi.org/10.2485/jhtb.31.245","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-148a-3p Regulates Stem Cell Osteogenic Differentiation and Enamel Development by Targeting Runt-Related Transcription Factor 2 and E-cadherin via the Wnt1/β-catenin Signaling Pathway","authors":"Huai-chun Chang, Tingting Jiang, Liang Kou, Duo Li, Xinchen Yu, Youqin Li, Lei Zhang","doi":"10.2485/jhtb.31.141","DOIUrl":"https://doi.org/10.2485/jhtb.31.141","url":null,"abstract":": We aimed to evaluate the regulatory effects of miR-148a-3p on stem cell osteogenic differentiation and enamel development by targeting runt-related transcription factor 2 (RUNX2) and E-cadherin, respectively. TargetScan software was utilized to predict the binding sites between miR-148a-3p and osteogenic marker gene RUNX2 or E-cadherin. The changes in miR-148a-3p expression during osteogenic differentiation of epidermal stem cells were detected. After transfec tion with miR-148a-3p mimics or miR-148a-3p inhibitor, epidermal stem cells were induced towards osteogenic differentia tion, and the changes in RUNX2 expression were measured. The changes in miR-148a-3p expression during enamel devel opment regulated by epidermal stem cells were determined. After liposome-mediated transfection with miR-148a-3p mimics or miR-148a-3p inhibitor, epidermal stem cells were induced towards ameloblast development. Cell proliferation and apoptosis abilities were tested using methyl thiazolyl tetrazolium assay and flow cytometry, respectively. The expres sion of miR-148a-3p was detected by RT-PCR. The protein expressions of Wnt1, β-catenin, RUNX2 and E-cadherin were measured by Western blotting. Epidermal stem cells differentiated into osteoblasts through osteogenic induction culture. On 5, 12, 15 and 30 d, epidermal stem cells gradually differentiated into osteoblasts through epithelial aggregation and depres sion, mesenchymal aggregation, and dentin and enamel secretion. After transfection, compared with negative control (NC) group, the cell viability of miR-148-3p group significantly decreased (P<0.05), and the apoptosis rate increased (P<0.01). The viability of miR-148-3p inhibitor group significantly increased (P<0.05), while the apoptosis rate reduced (P<0.01). The dual-luciferase reporter assay showed that miR-148a-3p targeted Wnt1. Compared with NC group, the expression of miR-148a-3p significantly rose in miR-148a-3p group (P<0.001), and the protein expression levels of Wnt1, β-catenin, RUNX2 and E-cadherin significantly decreased. Compared with NC group, the expression of miR-148a-3p in miR-148a-3p inhibitor group significantly decreased (P<0.05), and the protein expression levels of Wnt1, β-catenin, RUNX2 and E-cad herin significantly increased. MiR-148a-3p is highly expressed in and regulates osteogenic differentiation and enamel de velopment through targeting RUNX2 and E-cadherin respectively via the Wnt1/β-catenin pathway, which plays an impor tant role in cell differentiation, stem cell proliferation and enamel development.","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-Dimensional Observation of the Furcation Area during Multi-Rooted Tooth Formation in Rat","authors":"Nobue Kikuchi, Kei Kitamura, N. Kasahara, Yudai Ogawa, N. Ishikawa, Masahito Yamamoto, Hitoshi Yamamoto","doi":"10.2485/jhtb.31.163","DOIUrl":"https://doi.org/10.2485/jhtb.31.163","url":null,"abstract":": Epithelial projections (EP), which form as Hertwig’s epithelial root sheath (HERS) extends, play an important role in the formation of multi-rooted teeth. However, three-dimensional (3D) observations of the process in which EP form the furcation area have not yet been conducted. To investigate the formation of the furcation area by EP, we used a 3D reconstruction model of the furcation area in rats, which form subpulpal lobes in the same manner as humans, and examined the extent of cell division around the epithelium. Wistar rats between postnatal days 3-18 (PN3-18) (n=3/age) were fixed by the perfusion of 4% paraformaldehyde solution, and the maxillae were dissected out and decalcified. Tissues were embed ded in paraffin by the conventional protocol, and 5-µm-thick serial sections and some sections for immunohistochemistry using an anti-proliferating cell nuclear antigen antibody of the frontal plane were prepared. All serial sections were stained by hematoxylin and eosin, and the structures of HERS, EP, dentin projections, and subpulpal lobes were overlayed after de-lineation every 5 sections from the images obtained of the maxillary second molar for the 3D reconstruction using ITK-snap software. Reconstructed images showed that a portion of HERS in the cervical region had extended into the dental papilla on PN3 to form EP. Four EP formed and extended with age at different speeds. On PN11, EP fused with one another in the center of the dental papilla and started to form subpulpal lobes. During this process, apical elongation in the apical direction of EP was not observed. Significant cell proliferation was detected in the dental papilla around the epithelium of the cervical loop. Collectively, these results indicate that the subpulpal lobes form immediately after the fusion of EP, and HERS extend in the apical direction following the fusion of EP.","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DOT1L Epigenetically Induces the Expression Level of FoxM1 through H3K79me2 and Affects the Malignant Behaviors of Head and Neck Squamous Cell Carcinoma Cells","authors":"Huanle Du, Lin Zhang","doi":"10.2485/jhtb.31.101","DOIUrl":"https://doi.org/10.2485/jhtb.31.101","url":null,"abstract":"DOT1-like histone lysine methyltransferase (DOT1L) has been revealed to be highly correlated with progression of diverse types of cancer, while its role in head and neck squamous cell carcinoma (HNSCC) has still remained elusive. In this study, it was found that DOT1L was highly expressed in HNSCC tissues and cell lines, and its high expression level was closely associated with poor prognosis, tumor differentiation, T stage, and pathological stage of HNSCC patients. With the introduction of DOT1L inhibitors, SGC 0946, DOT1L inhibition significantly suppressed proliferation, migration, and invasion of HNSCC cells, while increased their apoptosis rate. To investigate the fundamental mechanism, we predicted that the expression level of Forkhead Box M1 (FoxM1) was positively correlated with DOT1L, and it was found that the expression level of FoxM1 was upregulated in HNSCC tissues and cell lines. Importantly, DOT1L activated FoxM1 through H3K79me2, and inhibition of DOT1L repressed the malignant behaviors of HNSC cells, which could be rescued by FoxM1 overexpression. Taken together, the results suggested that DOT1L could epigenetically induce the expression level of FoxM1 through H3K79me2 and affected the malignant behaviors of HNSCC cells, which could provide novel outcomes for the therapy of HNSC.","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"31 2","pages":"101-108"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.jstage.jst.go.jp/article/jhtb/31/2/31_101/_pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147899364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Super-Hydrophilic Treatment on Zirconia Implant Osseointegration in Rats","authors":"Tomoki Hirano, T. Miura, Yuto Otsu, Atsuro Harada, Y. Asami, Noriko Iijima, Yukari Oda, Yoshitaka Furuya, Taichi Ito, Hodaka Sasaki, H. Sekine","doi":"10.2485/jhtb.31.223","DOIUrl":"https://doi.org/10.2485/jhtb.31.223","url":null,"abstract":": Surface modifications of implants can improve the rate of osseointegration. The aim of this study was to deter mine the effect of super-hydrophilic modification on tetragonal zirconia polycrystals (TZP) implant surface and its subse quent effect on the rate of osseointegration. The TZP implants were rendered super-hydrophilic by the use of ultraviolet light (UV) or via atmospheric-pressure plasma treatments (PL), on their surface and were compared to control specimen that any surface modification wasn’t performed (NC). According to the surface wettability and x-ray photoelectron spec troscopy (XPS) analysis, the contact angle of water droplets on the surface of UV and PL was 0 degree, and their C1s peak was less than that of NC. The push-in test and histological analysis revealed that the super-hydrophilic modification en hanced the bone-implant integration and the formation of new bone around the TZP implants. Additionally, carbon removal and surface wettability enhancement likely improved the osseointegration rate. The study, therefore, demonstrates the de-sign of future TZP implants, particularly for dental applications. This study explored the effect of super-hydrophilic surface modifica tion on TZP implants and its subsequent effect on the rate of osseointe gration in rats. In this study, UV and PL were used. These treatments had no detrimental effect on the TZP implant surface and demonstrated sufficient capacity for carbon removal and super-hydrophilicity for TZP implants. UV and PL TZP implants demonstrated improved osseointe gration rate compared to NC. Super-hydrophilic surface treatments have been used to improve the osseointegration rate of titanium implants. Ultraviolet light exposure en hances protein adsorption, bone marrow cell adhesion, osteoblast differ -entiation, and osseointegration rate of titanium implants 12) . A previous study demonstrated that 4-week-aged, Ultraviolet light treated titanium surfaces increased bioactivity to a level comparable to or greater than that of freshly prepared surfaces, owing to the restoration of super-hy-drophilicity 13) . Plasma-treated titanium surfaces promoted osteoblast proliferation 14) and increased bone-to-implant contact 15,16) . Ultraviolet light treatment enhanced osteoblast adhesion, proliferation, and mineralization in zirconia implants 22) and improved bone-to-implant contact and osseointegration 23) . Additionally, Plasma treatment enhanced the bio-compatibility of zirconia implants 24–26) . The findings of this study are consistent with those reported in the previous studies.","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of TGF-β on Growth and Invasion of Human Oral Squamous Cell Carcinoma Cell Lines","authors":"Hitoe Ishiguro-Katsuta, Y. Okada","doi":"10.2485/jhtb.31.171","DOIUrl":"https://doi.org/10.2485/jhtb.31.171","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"microRNA-324-3p Promotes Osteoblasts Differentiation via Suppressing SMAD7","authors":"Wei Xu, Rui Xia, Feng Tian, Lei Liu, Mengmeng Li, Shi-yuan Fang","doi":"10.2485/jhtb.31.263","DOIUrl":"https://doi.org/10.2485/jhtb.31.263","url":null,"abstract":": Fracture healing is a complex dynamic process that involves the balance between osteoblasts and osteoclasts. Sev-eral microRNAs (miRNAs) have been shown to participate in fracture healing. In this study, we investigated the role of miR-324-3p in osteoblast differentiation. MC3T3-E1 cell differentiation was induced by icariin, and miR-324-3p expression levels during cell differentiation were measured using qRT-PCR. Cell proliferation and differentiation were assessed to eval uate the function of miR-324-3p. Luciferase activity was used for target gene verification. During MC3T3-E1 differentia tion, miR-324-3p levels gradually increased over time. Further experiments showed that miR-324-3p overexpression significantly promoted cell viability, whereas miR-324-3p downregulation showed the opposite effect. For cells with miR-324-3p mimic, the levels of bone sialoprotein, Runx2, osteocalcin, and alkaline phosphatase activity were significantly elevated. SMAD7 is the target gene of miR-324-3p, and its level is gradually downregulated during MC3T3-E1 cell differentiation. MiR-324-3p may promote MC3T3-E1 cell differentiation by targeting SMAD7.","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical Observation on the Distribution and Morphological Changes of GAP-43 Positive Structures in the Formation of Experimental Apical Periodontitis of Rat Molars","authors":"Seiko Tanzawa, Kei Kitamura, N. Ishikawa, Yoshiki Tamiya, R. Sako, M. Furusawa, Hitoshi Yamamoto","doi":"10.2485/jhtb.31.155","DOIUrl":"https://doi.org/10.2485/jhtb.31.155","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}