Journal of Immunoassay and Immunochemistry最新文献_第5页

Seroprevalence of Toxoplasma Gondii antibodies in sheep and goats slaughtered at the Kumasi Abattoir, Ghana 加纳库马西屠宰场屠宰的绵羊和山羊中弓形虫抗体的血清阳性率

Journal of Immunoassay and Immunochemistry Pub Date : 2019-07-18 DOI: 10.1080/15321819.2019.1642916

K. Bentum, R. Folitse, E. Amemor, V. Burimuah, T. Opoku-Agyemang, B. Emikpe

{"title":"Seroprevalence of Toxoplasma Gondii antibodies in sheep and goats slaughtered at the Kumasi Abattoir, Ghana","authors":"K. Bentum, R. Folitse, E. Amemor, V. Burimuah, T. Opoku-Agyemang, B. Emikpe","doi":"10.1080/15321819.2019.1642916","DOIUrl":"https://doi.org/10.1080/15321819.2019.1642916","url":null,"abstract":"ABSTRACT Toxoplasmosis, caused by T. gondii, is an important zoonosis worldwide. In Ghana, information on the disease in humans abounds but scanty in animals. This study was therefore conducted to estimate the seroprevalence of T. gondii infection sheep and goats sampled from the Kumasi Abattoir in Ashanti Region, Ghana. A total of 347 serum samples collected from 170 sheep and 177 goats were analyzed for the presence of T. gondii antibodies using a commercial ELISA kit. Results of this study estimated the seroprevalence of 23.7% in goats an, 35.9% in sheep. In sheep, 24 (35.82%) out of a total of 67 male samples were positive and 37 (35.92%) out of a total of 104 female samples were positive while in goats, 6 (8.2%) bucks out of a total of 73 were positive while 36 (34.6%) does out of a total of 104 were positive. There was a significant difference in the rate of seropositivity of female goats (p-value 0.01). This study confirms the existence of T. gondii infection in small ruminants in Ghana and it showed that sheep and dogs are more at risk to T. gondii infection hence meat from such animals could be a potential risk to public health if consumed raw or undercooked.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"25 1","pages":"495 - 501"},"PeriodicalIF":0.0,"publicationDate":"2019-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86843441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

EOV "Editorial Board" EOV“编辑委员会”

Journal of Immunoassay and Immunochemistry Pub Date : 2017-11-02 DOI: 10.1080/15321819.2017.1411438

引用次数: 0

Comparison of two competitive enzyme immunoassay kits for quantification of plasma Urotensin-II in rats 两种竞争性酶免疫测定试剂盒测定大鼠血浆尿紧张素- ii的比较

Journal of Immunoassay and Immunochemistry Pub Date : 2017-05-04 DOI: 10.1080/15321819.2016.1250772

A. Rodríguez-Rodríguez, J. Egea-Guerrero, Á. Vilches-Arenas, Manuel Jesús Quintanilla-Vázquez, F. Murillo-Cabezas, M. Muñoz-Sanchez

引用次数: 2

A multi-analyte immunoassay for thyroid related analytes 甲状腺相关分析物的多分析物免疫分析

Journal of Immunoassay and Immunochemistry Pub Date : 2017-05-04 DOI: 10.1080/15321819.2016.1250771

B. Jain, J. Kumarasamy, C. Gholve, S. Kulkarni, M. Rajan

引用次数: 4

Eliciting an antibody response against a recombinant TSH containing fusion protein 诱导对含有融合蛋白的重组TSH的抗体反应

Journal of Immunoassay and Immunochemistry Pub Date : 2017-05-04 DOI: 10.1080/15321819.2016.1250774

Maysam Mard-Soltani, M. Rasaee, A. Sheikhi, M. Hedayati

引用次数: 6

Immunoassay and molecular methods to investigate DNA methylation changes in peripheral blood mononuclear cells in HIV infected patients on cART 免疫测定和分子方法研究cART感染HIV患者外周血单个核细胞DNA甲基化变化

Journal of Immunoassay and Immunochemistry Pub Date : 2017-05-04 DOI: 10.1080/15321819.2016.1260587

A. Roșca, G. Anton, L. Ene, I. Iancu, A. Temereanca, C. Achim, S. Ruță

引用次数: 7

Measuring fecal testosterone metabolites in spotted hyenas: Choosing the wrong assay may lead to erroneous results 测量斑点鬣狗的粪便睾酮代谢物:选择错误的测定方法可能导致错误的结果

Journal of Immunoassay and Immunochemistry Pub Date : 2017-05-04 DOI: 10.1080/15321819.2016.1260584

Susanne Pribbenow, T. G. Shrivastav, M. Dehnhard

引用次数: 6

Spontaneous mouse mammary tumor cell lysates induce IgG production in spleen mononuclear cells of healthy and tumor-bearing mice 自发性小鼠乳腺肿瘤细胞裂解物诱导健康小鼠和荷瘤小鼠脾单核细胞产生IgG

Journal of Immunoassay and Immunochemistry Pub Date : 2017-05-04 DOI: 10.1080/15321819.2016.1266499

Ahad Khalilnezhad, Elham Mahmoudian, N. Mosaffa, J. Mohsenifar, D. Amani

{"title":"Spontaneous mouse mammary tumor cell lysates induce IgG production in spleen mononuclear cells of healthy and tumor-bearing mice","authors":"Ahad Khalilnezhad, Elham Mahmoudian, N. Mosaffa, J. Mohsenifar, D. Amani","doi":"10.1080/15321819.2016.1266499","DOIUrl":"https://doi.org/10.1080/15321819.2016.1266499","url":null,"abstract":"ABSTRACT Background. We hypothesized that Tumor cell lysate (TCL) prepared from spontaneous mouse mammary tumor (SMMT) may elicit IgG production by spleen mononuclear cells (SMCs) in ex vivo. Methods. The SMCs from healthy mice (HM, n = 6) and four-week SMMT-bearing mice (TBM, n = 6) was cultured in presence of TCL and mitogen for 42 hr at 37°C, separately. Serum and SMCs culture supernatant levels of IFNγ, IL-4, and total IgG were measured using special ELISA kits. Results. Serum IgG level of TBM was significantly higher than that of HM group (P = 0.019), while serum IFNγ and IL-4 did not differ between two groups (P > 0.05). Mitogen significantly induced ex vivo production of both IFNγ (P = 0.013) and IL-4 (P = 0.015) by SMCs from HM group, and only IL-4 (P = 0.049) by SMCs from TBM group. In contrast, TCL increased ex vivo production of IgG by SMCs from both HM (P = 0.034) and TBM (P = 0.016) groups. The ex vivo IgG revealed a moderate positive correlation with tumor size (r = 0.578, P = 0.422). Conclusion. It seems that TCL prepared from SMMT are potent inducers of IgG production. This may propose TCL as a potential tool for monitoring of humoral immunity in animal models.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"1 1","pages":"333 - 342"},"PeriodicalIF":0.0,"publicationDate":"2017-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82436208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

C3:CH50 ratio as a proposed composite marker for eculizumab monitoring in atypical hemolytic uremic syndrome: Preliminary results C3:CH50比值作为eculizumab监测非典型溶血性尿毒症综合征的复合标记物:初步结果

Journal of Immunoassay and Immunochemistry Pub Date : 2017-03-04 DOI: 10.1080/15321819.2016.1234485

K. Kerboua, F. Haiba, D. Batouche

{"title":"C3:CH50 ratio as a proposed composite marker for eculizumab monitoring in atypical hemolytic uremic syndrome: Preliminary results","authors":"K. Kerboua, F. Haiba, D. Batouche","doi":"10.1080/15321819.2016.1234485","DOIUrl":"https://doi.org/10.1080/15321819.2016.1234485","url":null,"abstract":"ABSTRACT Treatment of atypical hemolytic uremic syndrome (aHUS) by the complement C5 inhibitor eculizumab (Soliris®) is highly effective but unfortunately, associated with an economic pressure on the health care systems even in high incomes countries. Despite spacing infusions having been proposed as the unique solution to minimize this economic impact, no reliable laboratory assays are available to tailor such therapy optimization. We aimed to propose and evaluate a complement composite marker for eculizumab efficacy monitoring in aHUS. We have retrospectively analyzed complement profiles data of eight aHUS patients under eculizumab from the International Registry of HUS/Thrombotic Thrombocytopenia Purpura, and calculated a novel marker “C3:CH50 ratio” by dividing C3 value by CH50 one for each sample during induction and maintenance periods. The results significance was compared to the currently used biomarkers for eculizumab tailoring. In contrast to the current biomarkers used for eculizumab efficacy monitoring like CH50 and soluble or deposit membrane attack complexes, “C3:CH50 ratio” seems to be the most interesting one since its value at pre-eculizumab dosage equaled 0.92 ± 0.2 while the post-eculizumab one increased significantly to reach 24.54 ± 10.7; P < 0.001. Furthermore, this ratio correlated negatively with platelets count (r = −0.722, P = 0.018) while no correlation was found within the thrombotic microangiopathy (TMA) biomarkers and complement blockade for the other parameters that change in pre and post-eculizumab therapy. As far as we know, this is the first study that suggests a post-eculizumab parameter correlating simultaneously with drug’s activity (complement inhibition) and disease activity (platelets counts). Nonetheless, the limited number of patients enrolled in this study should be considered in larger studies to guide eculizumab optimization by indicating the time when subsequent withdrawal or infusion spacing is allowed or recommended.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"29 1","pages":"178 - 189"},"PeriodicalIF":0.0,"publicationDate":"2017-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73706426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

An enzyme immunoassay for determining epidermal growth factor (EGF) in human serum samples using an ultramicroanalytical system 用超微量分析系统测定人血清样品中表皮生长因子(EGF)的酶免疫测定

Journal of Immunoassay and Immunochemistry Pub Date : 2017-03-04 DOI: 10.1080/15321819.2016.1236729

Elisa M. Castells Martínez, Ruben Del Valle, E. C. González, A. Melchor, P. L. Pérez, Idania González, A. Carr, K. Leon

{"title":"An enzyme immunoassay for determining epidermal growth factor (EGF) in human serum samples using an ultramicroanalytical system","authors":"Elisa M. Castells Martínez, Ruben Del Valle, E. C. González, A. Melchor, P. L. Pérez, Idania González, A. Carr, K. Leon","doi":"10.1080/15321819.2016.1236729","DOIUrl":"https://doi.org/10.1080/15321819.2016.1236729","url":null,"abstract":"ABSTRACT Human epidermal growth factor is a small peptide consisting of 53 amino acid residues, which stimulates cell proliferation and is associated with several human carcinomas. A simple sandwich-type ultramicroELISA assay (UMELISA), based on the advantages of high affinity reaction between streptavidin and biotin has been developed for the measurement of EGF in human serum samples. Strips coated with a high affinity monoclonal antibody directed against EGF are used as solid phase, to ensure the specificity of the assay. The EGF assay was completed in 18 hr, with a measuring range of 39–2500 pg/mL. The intra- and inter-assay coefficients of variation were 4.4–7.3% and 0–5.1%, respectively, depending on the EGF concentrations evaluated. Percentage recovery ranged from 96–104%. Regression analysis showed a good correlation with the commercially available Human EGF Immunoassay Quantikine® ELISA kit (n = 130, r = 0.92, P < 0.01). The analytical performance characteristics of our UMELISA EGF endorse its use for the quantification of EGF in human serum samples.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"1 1","pages":"190 - 201"},"PeriodicalIF":0.0,"publicationDate":"2017-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89948991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8