Journal of Immunoassay and Immunochemistry最新文献_第6页

Prevalence of anti-hepatitis C virus antibody among pregnant women and blood donors at Bowen University Teaching Hospital, Ogbomoso, Oyo State, Nigeria 尼日利亚奥约州Ogbomoso的Bowen大学教学医院孕妇和献血者中抗丙型肝炎病毒抗体的流行情况

Journal of Immunoassay and Immunochemistry Pub Date : 2017-03-04 DOI: 10.1080/15321819.2016.1241264

A. Hilda, O. J. Kola, O. E. Kolawole

{"title":"Prevalence of anti-hepatitis C virus antibody among pregnant women and blood donors at Bowen University Teaching Hospital, Ogbomoso, Oyo State, Nigeria","authors":"A. Hilda, O. J. Kola, O. E. Kolawole","doi":"10.1080/15321819.2016.1241264","DOIUrl":"https://doi.org/10.1080/15321819.2016.1241264","url":null,"abstract":"ABSTRACT Hepatitis C virus is one of the emerging infectious diseases that can be transmitted through blood-to-blood contact. This study was carried out to determine the prevalence of anti-HCV antibodies among potential blood donors and pregnant women attending Bowen University Teaching Hospital (BUTH), Ogbomoso, Oyo State. This hospital-based study was conducted from December 2014 to September 2015. The study group (N = 279) included potential blood donors and pregnant women. Data on socio-demographic characteristics and potential risk factors were collected using a structured questionnaire. The presence of anti-HCV antibodies in serum samples of the studied subjects were detected using third-generation Enzyme Linked Immunosorbent Assay (ELISA) (WKEA Med Supplies Corp, China). Chisquare test was utilized to assess the association between the socio-demographic variables and HCV status. Logistic regression was done to determine the strength of association between risk factors and HCV status. Statistical significance was set at P ˂ 0.05. Overall seroprevalence of hepatitis C virus infection was found to be 1.79% consisting 0.36% of pregnant women and 1.43% of blood donors. None of the socio-demographic characteristics and potential risk factors among the study groups were significantly associated with hepatitis C virus infection. This study found a seroprevalence of anti-HCV antibody to be 1.79%, thus, screening of pregnant women and blood donors for HCV infections with the use of ELISA is recommended because of its important role in detecting the presence of anti-HCV antibody with utmost specificity and sensitivity.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"61 1","pages":"221 - 234"},"PeriodicalIF":0.0,"publicationDate":"2017-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84807253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Establishment of a reference ELISA for measurement of universal thresholds of pregnanediol glucuronide excretion rates using urine samples diluted to a constant volume per unit time 用单位时间内稀释至定容的尿液样品，建立测定妊娠二醇葡萄糖醛酸酯排泄率通用阈值的参考ELISA方法

Journal of Immunoassay and Immunochemistry Pub Date : 2017-03-04 DOI: 10.1080/15321819.2016.1236730

J. Binnie, D. Cooke, L. Blackwell

{"title":"Establishment of a reference ELISA for measurement of universal thresholds of pregnanediol glucuronide excretion rates using urine samples diluted to a constant volume per unit time","authors":"J. Binnie, D. Cooke, L. Blackwell","doi":"10.1080/15321819.2016.1236730","DOIUrl":"https://doi.org/10.1080/15321819.2016.1236730","url":null,"abstract":"ABSTRACT An enzyme-linked immunosorbent assay (ELISA) for measurement of pregnanediol-3α-glucuronide (PdG) excretion rates in urine samples diluted to 150 mL/h before analysis is described. The sensitivity of the 9 optimized standard curves was 0.093 ± 0.070 μmol PdG/24 hr, with the multiple combined standard curves having a mean mid-point (EC50) of 6.88 μmol PdG/24 hr. The PdG threshold excretion rate of 7.0 μmol/24 hr, which is used as a marker for the end of fertility, was situated in the most accurate region of the standard curve. The specificity of the ELISA was determined using normal variate transformation to compare seven menstrual cycle profiles obtained with the ELISA method with the profiles obtained previously using a validated radioimmunoassay (RIA) method. The cycle profiles all agreed within experimental error, and a high degree of correlation using Deming regression was obtained. The correlation equation was Y = 1.57X-0.11 μmol PdG/24 hr (n = 200; r = 0.932). The PdG excretion rates determined by the ELISA were 50% higher than given by RIA, but the normal ranges were similar to those given by the original reference gas liquid chromatographic method. The ELISA assay was therefore suitable as a reference method for measurement of thresholds of PdG excretion rates.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"270 1-2 1","pages":"202 - 220"},"PeriodicalIF":0.0,"publicationDate":"2017-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78453769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Development of an immunoassay using an anti-wogonin glucuronide monoclonal antibody 抗枸杞苷葡萄糖醛酸单克隆抗体免疫测定方法的建立

Journal of Immunoassay and Immunochemistry Pub Date : 2017-02-28 DOI: 10.1080/15321819.2016.1273236

M. K. Paudel, S. Sakamoto, L. Huy, Hiroyuki Tanaka, T. Miyamoto, A. Takano, S. Morimoto

引用次数: 3

Evaluation of immune response in challenged chickens vaccinated with Newcastle disease vaccine using gums from Cedrela odorata and Khaya senegalensis as delivery agents 以香树胶和塞内加尔香树胶为递送剂接种新城疫疫苗的攻毒鸡免疫应答评价

Journal of Immunoassay and Immunochemistry Pub Date : 2017-01-25 DOI: 10.1080/15321819.2016.1273237

V. Oyebanji, B. Emikpe, A. O. Omolade, M. O. Odeniyi, A. Salami, O. I. Osowole, O. Kasali, O. Akinboade

{"title":"Evaluation of immune response in challenged chickens vaccinated with Newcastle disease vaccine using gums from Cedrela odorata and Khaya senegalensis as delivery agents","authors":"V. Oyebanji, B. Emikpe, A. O. Omolade, M. O. Odeniyi, A. Salami, O. I. Osowole, O. Kasali, O. Akinboade","doi":"10.1080/15321819.2016.1273237","DOIUrl":"https://doi.org/10.1080/15321819.2016.1273237","url":null,"abstract":"ABSTRACT Immune response of challenged chickens following previous vaccinations with Newcastle disease vaccine using gums from Cedrela odorata and Khaya senegalensis as delivery agent were evaluated. Two hundred and fifty-two one-day old chickens were divided into vaccine-gum oral (GVOR), vaccine-gum ocular (GVOC), vaccine oral (VOR), vaccine ocular (VOC), gum oral (GOR), gum ocular (GOC), No-gum-no-vaccine/challenged (NGNV/C), and No-gum-no-vaccine/unchallenged (NGNV/U) groups. They were vaccinated at days 21 & 42 and challenged at day 84. Trachea and intestinal washings were collected at intervals as well as weekly serum samples. These were analyzed using enzyme-linked immunosorbent assay (ELISA) and haemagglutination inhibition (HI) test for mucosal and systemic IgG response (MA and SA). Statistical analysis was done using Omnibus one-way ANOVA. MA and SA were not different (P > 0.05) post first and second vaccination although gum-vaccine groups were marginally higher post second vaccination. Post Infection (PI), there was an early and sustained spike in both MA and SA for the GV groups especially GVOR (P < 0.05). MA and SA for the Gum alone (especially GOR) groups also spiked PI (P < 0.05). Therefore, phytogenic polymers used could be said to possess immunopotentiating property with a possible induction of immunologic memory mechanism.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"18 1","pages":"378 - 388"},"PeriodicalIF":0.0,"publicationDate":"2017-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75124016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Scale-up production and characterization of anti-human cardiac troponin I monoclonal antibody in ascitic fluid of balb/c mice balb/c小鼠腹水中抗人心肌肌钙蛋白I单克隆抗体的规模化生产及鉴定

Journal of Immunoassay and Immunochemistry Pub Date : 2017-01-25 DOI: 10.1080/15321819.2016.1274263

Asiabanha Rezaee Majid, RasaeeMohammad Javad, Paknejad Malihe, Mohammadnejad Javad

{"title":"Scale-up production and characterization of anti-human cardiac troponin I monoclonal antibody in ascitic fluid of balb/c mice","authors":"Asiabanha Rezaee Majid, RasaeeMohammad Javad, Paknejad Malihe, Mohammadnejad Javad","doi":"10.1080/15321819.2016.1274263","DOIUrl":"https://doi.org/10.1080/15321819.2016.1274263","url":null,"abstract":"ABSTRACT The Human Cardiac Troponin I (hcTnI) is a 210 amino acid protein, 23 kDa in molecular weight. This biomarker is commonly used to diagnose myocardial infarction, micro injury, and acute coronary syndrome (ACS) in patients referring to emergency departments. The American Heart Association (AHA) and European Society of Cardiology (ESC) proposed troponin I as the gold biomarker for early detection of heart attack, especially in myocardial infarction (MI). Therefore, developing monoclonal antibodies against this biomarker could help in for early detection of heart attack. Hybridoma technology is a well-known technique introduced to produce monoclonal antibodies in specialized cells. The aim of this study was to produce large scale of monoclonal antibody against human cardiac troponin I using Hybridoma technology in order to design a diagnostic kit. The monoclonal antibody was produced using conventional Hybridoma technology in ascitic fluid of mouse and characterized for its ability to detect Human Cardiac Troponin I in a rapid test system. The results indicate the successful detection of Troponin I using the obtained monoclonal antibody. According to the achieved results it seems that ascites production of monoclonal antibody is very versatile, inexpensive, and economically useful for monoclonal antibody production.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":"78 1","pages":"389 - 399"},"PeriodicalIF":0.0,"publicationDate":"2017-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90853590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Simultaneous determination of glycyrrhizin and liquiritin in licorice roots and Kampo medicines by combination enzyme-linked immunosorbent assay using anti-glycyrrhizin and anti-liquiritin monoclonal antibodies 用抗甘草酸苷和抗甘草酸苷单克隆抗体联合酶联免疫吸附法同时测定甘草和汉方药中甘草酸苷和甘草苷的含量

Journal of Immunoassay and Immunochemistry Pub Date : 2017-01-04 DOI: 10.1080/15321819.2016.1260586

Shunsuke Fujii, O. Morinaga, T. Uto, Shuichi Nomura, Y. Shoyama

引用次数: 9

Selection of specific inhibitor peptides in enzyme-linked immunosorbent assay (ELISA) of cardiac troponin I using immuno-dominant epitopes as competitor 以免疫显性表位为竞争对手的心肌肌钙蛋白I酶联免疫吸附试验(ELISA)中特异性抑制肽的选择

Journal of Immunoassay and Immunochemistry Pub Date : 2017-01-02 DOI: 10.1080/15321819.2016.1216444

M. Rezaee, M. Rasaee, J. Mohammadnejad

引用次数: 15

A new covalent peroxidase conjugation method using bis(sulfosuccinimidyl) suberate as cross-linking reagent in a two-step procedure 以双(磺基琥珀酰亚基)亚磺酸为交联试剂，两步法建立了一种新的共价过氧化物酶偶联方法

Journal of Immunoassay and Immunochemistry Pub Date : 2017-01-02 DOI: 10.1080/15321819.2016.1250773

R. Presentini

引用次数: 3

Highlights of new immunoassay-based technologies 基于免疫分析的新技术亮点

Journal of Immunoassay and Immunochemistry Pub Date : 2017-01-02 DOI: 10.1080/15321819.2016.1277320

C. Tanase, R. Albulescu, M. Neagu

引用次数: 2

EOV Editorial Board EOV编委会

Journal of Immunoassay and Immunochemistry Pub Date : 2016-11-01 DOI: 10.1080/15321819.2016.1222162

引用次数: 0