Journal of Immunoassay and Immunochemistry最新文献_第2页

Prevalence and risk factors of hepatitis E virus infection among patients with β-thalassemia major in South of Iran 伊朗南部β-地中海贫血患者戊型肝炎病毒感染的流行及危险因素

Journal of Immunoassay and Immunochemistry Pub Date : 2022-03-01 DOI: 10.1080/15321819.2022.2046602

F. Farshadpour, R. Taherkhani, Marziyeh Shaeri

{"title":"Prevalence and risk factors of hepatitis E virus infection among patients with β-thalassemia major in South of Iran","authors":"F. Farshadpour, R. Taherkhani, Marziyeh Shaeri","doi":"10.1080/15321819.2022.2046602","DOIUrl":"https://doi.org/10.1080/15321819.2022.2046602","url":null,"abstract":"ABSTRACT This study was performed to determine the prevalence and risk factors of hepatitis E virus (HEV) infection among thalassemia patients. All β-thalassemia major patients on regular blood transfusion attending the blood transfusion centers located in southern Iran were invited to participate in this study. ELISA was used to detect anti-HEV IgM and anti-HEV IgG antibodies. The molecular detection of HEV infection was performed by nested RT-PCR assay, targeting the ORF2 region of HEV genome, and sequencing. In this study, 16.67% of thalassemia patients were positive for anti-HEV IgG compared to 12.1% of the controls. Thalassemia patients were negative for anti-HEV IgM and HEV viremia. Patients with blood transfusion every two weeks had significantly higher anti-HEV IgG seroprevalence compared to the patients with blood transfusions at longer intervals (OR: 12.50; 95% CI: 1.76–88.74; P = .012). Anti-HEV IgG seroprevalence was not statistically associated with age, gender distribution, ethnicity, place of residency, education level, and serum levels of liver enzymes. This study reports a high seroprevalence of HEV among thalassemia patients, while frequency of blood transfusion was significantly associated with anti-HEV IgG seropositivity. This suggests that frequent blood transfusion may be a risk factor for exposure to HEV infection among thalassemia patients.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87939732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serosurvey of hepatitis A virus and E virus infection among municipal sweepers working in the largest city in the south of Iran 伊朗南部最大城市市政清洁工甲型肝炎病毒和戊型肝炎病毒感染的血清调查

Journal of Immunoassay and Immunochemistry Pub Date : 2021-07-16 DOI: 10.1080/15321819.2022.2052088

S. Hosseini, N. Joharinia, S. Hosseini, Saeed Firoozi Ghahestani, Rozita Ghojoghi, Kourosh Dorost, M. Moghadami, Abolfazl Gheshlaghi, O. Jaberi, Rohollah Khoshbakht, J. Sarvari

{"title":"Serosurvey of hepatitis A virus and E virus infection among municipal sweepers working in the largest city in the south of Iran","authors":"S. Hosseini, N. Joharinia, S. Hosseini, Saeed Firoozi Ghahestani, Rozita Ghojoghi, Kourosh Dorost, M. Moghadami, Abolfazl Gheshlaghi, O. Jaberi, Rohollah Khoshbakht, J. Sarvari","doi":"10.1080/15321819.2022.2052088","DOIUrl":"https://doi.org/10.1080/15321819.2022.2052088","url":null,"abstract":"ABSTRACT This study was conducted to determine the exposure rate of Hepatitis A and Hepatitis E viruses in urban solid waste collectors/sweepers in the south of Iran. The 385 samples (serums) were collected from Shiraz Municipality waste sweepers.. A questionnaire was used to gather data on their demographic and occupational characteristics, as well as their awareness of viral hepatitis disease. The viral seroprevalence was determined by commercial IgG ELISA kit. All participants were male, mean age of 41 ± 8 years. ELISA assay showed that all of them were positive for anti-HAV IgG. Also, 62 out of 385 individuals were positive for anti-HEV IgG. The statistical analysis showed that the frequency of HEV IgG antibody among age groups 20–30, 31–40, 41–50 and >50 years old had an increasing trend, 4.5%, 10.1%, 17.4%, and 36.7%, respectively, indicating age factor significance (p = .001). Based on some investigated factors including the duration of work experience, current and previous jobs, habitation, personal hygiene status, and knowledge on viral hepatitis diseasees/their transmission, there was no statistically significant difference between anti-HEV IgG positive versus negative sweepers. The results indicated a slighty higher frequency of anti-HAV and anti-HEV IgG among sweepers compared to other pre-investigated population. It doesn’t seem that garbage collecting/sweeping could be a significant risk factor for HAV and HEV infection.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81883856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

An update on investigations of autoimmune diseases affecting orofacial region 影响口面部的自身免疫性疾病的最新研究进展

Journal of Immunoassay and Immunochemistry Pub Date : 2020-01-02 DOI: 10.1080/15321819.2019.1680387

K. Garlapati, Mayuri Rallabandi, S. Shalini

引用次数: 0

Evaluation of the mucoadhesive strengths of Abelmoschus esculentus and Irvingia gabonensis gums for possible application in veterinary vaccine delivery: the effect of extraction methods 猪沙鼠和加蓬树胶黏附强度评价及其在兽医疫苗应用中的应用:提取方法的影响

Journal of Immunoassay and Immunochemistry Pub Date : 2020-01-02 DOI: 10.1080/15321819.2019.1680388

C. Ezeasor, B. Emikpe, M. O. Odeniyi, S. Shoyinka

{"title":"Evaluation of the mucoadhesive strengths of Abelmoschus esculentus and Irvingia gabonensis gums for possible application in veterinary vaccine delivery: the effect of extraction methods","authors":"C. Ezeasor, B. Emikpe, M. O. Odeniyi, S. Shoyinka","doi":"10.1080/15321819.2019.1680388","DOIUrl":"https://doi.org/10.1080/15321819.2019.1680388","url":null,"abstract":"ABSTRACT This study evaluates the effects of different gum extraction methods on the mucoadhesive strengths of Abelmoschus esculentus (AE) and Irvingia gabonensis (IG) gums and the release of vaccine antigen in vaccine–gum formulations. AE and IG gums were extracted employing previously documented methods with acetone or sodium chloride (NaCl) and either oven-dried or freeze-dried. Gum extracts were analyzed for mucoadhesive strengths using a modified rotational cylinder method on animal mucosa. The time taken to detach from the mucosa was taken as the Peak Adhesion Time (PAT). The gum extracts were charged with Peste des petits ruminant vaccine and the antigen release was evaluated using agar gel immunodiffusion technique. The means of the PATS were analyzed using Mann-whitney t-test at p < .05. The NaCl extracted and freeze-dried IG gum showed sustained mean PATs of 1766 ± 73 s; 2116 ± 101 s; 7044 ± 117 s, while the oven-dried IG gum and both AE gums showed short-lived average PATs. Vaccine-gum formulations of IG at ratios 2:1, 1:1 & 1:2 had strong positive reactions while only that of AE at 2:1 showed a strong positive reaction. This study shows that NaCl extracted and freeze-dried IG gum has immunomodulatory potential for mucoadhesive vaccine delivery in ruminants.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91502774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Cyclin D1 and PSA act as good prognostic and clinicopathological indicators for breast cancer Cyclin D1和PSA是乳腺癌良好的预后和临床病理指标

Journal of Immunoassay and Immunochemistry Pub Date : 2020-01-02 DOI: 10.1080/15321819.2019.1677706

N. Holah, A. Hemida

{"title":"Cyclin D1 and PSA act as good prognostic and clinicopathological indicators for breast cancer","authors":"N. Holah, A. Hemida","doi":"10.1080/15321819.2019.1677706","DOIUrl":"https://doi.org/10.1080/15321819.2019.1677706","url":null,"abstract":"ABSTRACT Breast cancer is one of the main causes of malignancies in females. Many prognostic parameters are verified but they do not give sufficient data about patients’ outcome. So, we must search for new prognostic and clinicopathological parameters. This study aimed to evaluate immunohistochemical expression of cyclin D1 and PSA in breast carcinoma and their relation to prognosis. It includes 79 specimens of breast invasive duct carcinoma. Overall survival time was available for all patients. There is a statistically significant association between positive PSA expression and lower tumor stage, nodal stage and tumor grade and between negative PSA expression and presence of metastasis (P value = .05, 0.01, 0.03 and 0.011, respectively). There is a highly statistically significant association between low percentage of cyclin D1 expression (<10%) and advanced tumor stage, advanced grade and presence of metastasis (P value = .002, 0.003 and 0.000, respectively) and between low and moderate percentage of Cyclin D1 expression (<10% and 10–50%) and advanced nodal stage (P value = .0.01). We concluded that Cyclin D1 and PSA were associated with good prognostic and clinicopathological parameters and therefore we suggested that they might be used as favorable prognostic indicators and should be analyzed in the context of molecular subtypes.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88137217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Effects of fructooligosaccharides (FOS) on growth, survival, gut microflora, stress, and immune response in Pacific white shrimp, Litopenaeus vannamei, cultured in a recirculating system 低聚果糖(FOS)对循环水养殖凡纳滨对虾生长、存活、肠道菌群、应激和免疫反应的影响

Journal of Immunoassay and Immunochemistry Pub Date : 2020-01-02 DOI: 10.1080/15321819.2019.1680386

A. Mustafa, A. Buentello, D. Gatlin, D. Lightner, M. Hume, A. Lawrence

引用次数: 11

Serum levels of anti-hepatitis B surface antibodies among vaccinated children aged 1 to 12 years in a rural community in Fars Province, southern Iran 伊朗南部法尔斯省一个农村社区接种疫苗的1至12岁儿童的血清抗乙型肝炎表面抗体水平

Journal of Immunoassay and Immunochemistry Pub Date : 2020-01-02 DOI: 10.1080/15321819.2019.1675696

N. Arefkhah, Sina Vafazadeh, Sepehr Shahriarirad, F. Ghorbani, S. Zoghi, M. Emami, Bardia Zamiri, M. Cheraghi, J. Sarvari, B. Sarkari

{"title":"Serum levels of anti-hepatitis B surface antibodies among vaccinated children aged 1 to 12 years in a rural community in Fars Province, southern Iran","authors":"N. Arefkhah, Sina Vafazadeh, Sepehr Shahriarirad, F. Ghorbani, S. Zoghi, M. Emami, Bardia Zamiri, M. Cheraghi, J. Sarvari, B. Sarkari","doi":"10.1080/15321819.2019.1675696","DOIUrl":"https://doi.org/10.1080/15321819.2019.1675696","url":null,"abstract":"ABSTRACT The present study aimed to find out the levels of anti-HBsAb among vaccinated children in a rural community in Fars Province, Southern Iran. Blood samples were taken from 550 children, aged 1–12 years (mean 6.4 ± 3.5), in 2017 from three villages in the area. A structured questionnaire was used to get the sociodemographic data of the subjects along with determinants concerning the Hepatitis B. Sera samples were examined for anti-HBsAb, using an ELISA commercial kit. Anti-HBsAb were detected in 468 (85.1%) of the subjects. Of the seropositive subjects, 37 (45.1%) were female and 45 (54.9%) were male. In the age group of 0–5 years, 88.7% of the subjects were seropositive. This rate was 84.3% and 78.1% in the age group of 6–10 years old and older than 10 years, respectively. There was a significant association (p < .05) between the anti-HBsAb and age. Findings of the current study revealed that children living in a rural community in southern Iran have appropriate protection against HBV even more than 10 years after being vaccinated. The decline in seropositivity rate of anti-HBsAb with age may further point out the need for a booster dose of HBV vaccine.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88017101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Seroprevalence and trend of hepatitides among blood donors in a district hospital in Ghana: a nine-year retrospective, descriptive cross-sectional study 加纳一家地区医院献血者中肝炎的血清阳性率和趋势:一项9年回顾性描述性横断面研究

Journal of Immunoassay and Immunochemistry Pub Date : 2020-01-02 DOI: 10.1080/15321819.2019.1682601

C. Nkansah, Dorcas Serwaa, Felix Osei-Boakye, Richard Owusu-Ampomah

{"title":"Seroprevalence and trend of hepatitides among blood donors in a district hospital in Ghana: a nine-year retrospective, descriptive cross-sectional study","authors":"C. Nkansah, Dorcas Serwaa, Felix Osei-Boakye, Richard Owusu-Ampomah","doi":"10.1080/15321819.2019.1682601","DOIUrl":"https://doi.org/10.1080/15321819.2019.1682601","url":null,"abstract":"ABSTRACT Hepatitis infections are among the greatest threats to human existence and survival and hence poses a serious public health challenge in blood donation. This study aimed to determine the prevalence and trend of hepatitis infections among voluntary blood donors at Nkenkaasu District Hospital. A retrospective study of voluntary blood donors’ records from January 2010 to December 2018 was conducted. Data on the donors’ age, sex and serum HBsAg and Hepatitis C virus results were retrieved from the Laboratory’s register and analyzed with SPSS version 16.0. Out of the 3306 total donors, the seroprevalence of hepatitis C infection was 11.7% and hepatitis B infection was 10.3%. Only 1.4% of the donors were co-infected with both pathogens and 82.7% had no infection. Rhesus blood group had a significant association with Hepatitis B (P = 0.005). As at 2010, both hepatitis infections had the same prevalence (73), from 2011 to 2018 both showed inconsistent trends. The relatively high seroprevalence of hepatitis infections identified from the study poses a greater threat to blood safety. Extensive screening of blood donors using standard techniques is highly recommended to ensure that recipients receive safe blood.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89758422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Comparison of two therapeutic approaches for the management of ventilator-associated pneumonia due to multidrug-resistant Acinetobacter: a randomized clinical trial study 多药耐药不动杆菌引起的呼吸机相关性肺炎两种治疗方法的比较:一项随机临床试验研究

Journal of Immunoassay and Immunochemistry Pub Date : 2019-11-28 DOI: 10.1080/15321819.2019.1696818

F. Khorvash, Shoeleh Yaghoubi, Shadi Farsaei, B. Ataei, A. Hakamifard, F. Mohajeri, Mohsen Gudarzi

{"title":"Comparison of two therapeutic approaches for the management of ventilator-associated pneumonia due to multidrug-resistant Acinetobacter: a randomized clinical trial study","authors":"F. Khorvash, Shoeleh Yaghoubi, Shadi Farsaei, B. Ataei, A. Hakamifard, F. Mohajeri, Mohsen Gudarzi","doi":"10.1080/15321819.2019.1696818","DOIUrl":"https://doi.org/10.1080/15321819.2019.1696818","url":null,"abstract":"ABSTRACT Management of ventilator-associated pneumonia (VAP) is a puzzling issue for infectious disease specialist. The present clinical trial study was aimed to comparing the effects of injectable colistin plus nebulized colistin and injectable colistin plus nebulized tobramycin on management of patients with VAP due to multidrug-resistant Acinetobacter. VAP patients were randomly divided into two groups (n = 30/each): Group 1 – patients that received intravenous (IV) meropenem, injectable colistin plus nebulized colistin, as a routine treatment, and Group 2 – patients that received IV meropenem, injectable colistin plus nebulized tobramycin. A total of 14 days of therapeutic intervention are required for every case. Follow-up for subjects was performed at five time-points: days 1, 3, 5, 7, and 14 after intervention. Also, a mean of creatinine levels of patients was determined in five times. In the present study, the clinical pulmonary infection score (CPIS) was determined on the basis of points assigned for various clinically manifestations of VAP. Based on our statistical analysis, there was no significant difference between CPIS and creatinine level in both Groups 1 and 2 (p > .05). CPIS and other clinical investigation appeared effectiveness of the treatment with injected colistin plus nebulized tobramycin; on the other hand, the results of present clinical trial showed that aforementioned therapeutic approach can be used as an alternative treatment for the management of infection in VAP patients.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81752291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Semi-solid phase assay for the alternative complement pathway activity assessment (AP100) 半固相法测定替代补体途径活性(AP100)

Journal of Immunoassay and Immunochemistry Pub Date : 2019-11-27 DOI: 10.1080/15321819.2019.1696819

K. Kerboua, K. Djenouhat

{"title":"Semi-solid phase assay for the alternative complement pathway activity assessment (AP100)","authors":"K. Kerboua, K. Djenouhat","doi":"10.1080/15321819.2019.1696819","DOIUrl":"https://doi.org/10.1080/15321819.2019.1696819","url":null,"abstract":"ABSTRACT Since the introduction of the most expensive drug in the world (Eculizumab) in the therapeutic arsenal of many diseases involving the alternative complement pathway (ACP) in their pathophysiology, the unmet need to perform simple ACP assays affordable for all countries has become one of the major challenges of the contemporary medicine. The assay currently used is AH50, despite it still challenging for several laboratories. This educational chapter consists of a detail protocol of standardized hemolytic assay AP100 and aims to help clinical laboratories over the world and especially those of the developing and low income countries to perform it. The procedure is essentially the same as for the timed lysis assay and dilution methods (AP50) except the concentration of ACP buffer and the chicken erythrocyte density used to make the gels. In clinical field, AP100 has at least nine applications in disease diagnosis and follow-up. AP100 has many advantages over the AH50 as it is more reliable for the Eculizumab monitoring and more practical with a purpose to be stored and transported for several weeks. AP100 is a portable and easy to use device both at the bedside and in the companion medical care.","PeriodicalId":15987,"journal":{"name":"Journal of Immunoassay and Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75684699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3