Journal of genetics and genomics = Yi chuan xue bao最新文献_第2页

Translation machinery: the basis of translational control. 翻译机制：翻译控制的基础。

Journal of genetics and genomics = Yi chuan xue bao Pub Date : 2024-04-01 Epub Date: 2023-08-01 DOI: 10.1016/j.jgg.2023.07.009

Shu Yuan, Guilong Zhou, Guoyong Xu

引用次数: 0

DAIS: a method for identifying spatial domains based on density clustering of spatial omics data. DAIS：基于空间 omics 数据密度聚类的空间域识别方法。

Journal of genetics and genomics = Yi chuan xue bao Pub Date : 2024-04-01 DOI: 10.1016/j.jgg.2024.04.002

Qichao Yu, Ru Tian, Xin Jin, Liang Wu

引用次数: 0

Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice. 含有38的卷曲螺旋结构域是小鼠顶体生物发生和纤维鞘组装所必需的。

Journal of genetics and genomics = Yi chuan xue bao Pub Date : 2024-04-01 Epub Date: 2023-09-13 DOI: 10.1016/j.jgg.2023.09.002

Yaling Wang, Xueying Huang, Guoying Sun, Jingwen Chen, Bangguo Wu, Jiahui Luo, Shuyan Tang, Peng Dai, Feng Zhang, Jinsong Li, Lingbo Wang

{"title":"Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice.","authors":"Yaling Wang, Xueying Huang, Guoying Sun, Jingwen Chen, Bangguo Wu, Jiahui Luo, Shuyan Tang, Peng Dai, Feng Zhang, Jinsong Li, Lingbo Wang","doi":"10.1016/j.jgg.2023.09.002","DOIUrl":"10.1016/j.jgg.2023.09.002","url":null,"abstract":"During spermiogenesis, haploid spermatids undergo dramatic morphological changes to form slender sperm flagella and cap-like acrosomes, which are required for successful fertilization. Severe deformities in flagella cause a male infertility syndrome, multiple morphological abnormalities of the flagella (MMAF), while acrosomal hypoplasia in some cases leads to sub-optimal embryonic developmental potential. However, evidence regarding the occurrence of acrosomal hypoplasia in MMAF is limited. Here, we report the generation of base-edited mice knocked out for coiled-coil domain-containing 38 (Ccdc38) via inducing a nonsense mutation and find that the males are infertile. The Ccdc38-KO sperm display acrosomal hypoplasia and typical MMAF phenotypes. We find that the acrosomal membrane is loosely anchored to the nucleus and fibrous sheaths are disorganized in Ccdc38-KO sperm. Further analyses reveal that Ccdc38 knockout causes a decreased level of TEKT3, a protein associated with acrosome biogenesis, in testes and an aberrant distribution of TEKT3 in sperm. We finally show that intracytoplasmic sperm injection overcomes Ccdc38-related infertility. Our study thus reveals a previously unknown role for CCDC38 in acrosome biogenesis and provides additional evidence for the occurrence of acrosomal hypoplasia in MMAF.","PeriodicalId":15985,"journal":{"name":"Journal of genetics and genomics = Yi chuan xue bao","volume":" ","pages":"407-418"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10246097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex-biased single-cell genetic landscape in mice with autism spectrum disorder. 自闭症谱系障碍小鼠的性别偏见单细胞遗传景观。

Journal of genetics and genomics = Yi chuan xue bao Pub Date : 2024-03-01 Epub Date: 2023-09-12 DOI: 10.1016/j.jgg.2023.08.012

Qian Zhang, Yongjie Wang, Jie Tao, Ruixue Xia, Yijie Zhang, Zhirui Liu, Jiwei Cheng

{"title":"Sex-biased single-cell genetic landscape in mice with autism spectrum disorder.","authors":"Qian Zhang, Yongjie Wang, Jie Tao, Ruixue Xia, Yijie Zhang, Zhirui Liu, Jiwei Cheng","doi":"10.1016/j.jgg.2023.08.012","DOIUrl":"10.1016/j.jgg.2023.08.012","url":null,"abstract":"Autistic spectrum disorder (ASD) is a male-biased, heterogeneous neurodevelopmental disorder that affects approximately 1%-2% of the population. Prenatal exposure to valproic acid (VPA) is a recognized risk factor for ASD, but the cellular and molecular basis of VPA-induced ASD at the single-cell resolution is unclear. Here, we aim to compare the cellular and molecular differences in the hippocampus between male and female prenatal mice with ASD at the single-cell transcriptomic level. The transcriptomes of more than 45,000 cells are assigned to 12 major cell types, including neurons, glial cells, vascular cells, and immune cells. Cell type-specific genes with altered expression after prenatal VPA exposure are analyzed, and the largest number of differentially expressed genes (DEGs) are found in neurons, choroid plexus epithelial cells, and microglia. In microglia, several pathways related to inflammation are found in both males and females, including the tumor necrosis factor (TNF), nuclear factor kappa B (NF-κB), toll-like receptor (TLR), and mitogen-activated protein kinase (MAPK) signaling pathways, which are important for the induction of autistic-like behavior. Additionally, we note that several X-linked genes, including Bex1, Bex3, and Gria3, were among the male-specific DEGs of neurons. This pioneering study describes the landscape of the transcriptome in the hippocampus of autistic mice. The elucidation of sexual differences could provide innovative strategies for the prevention and treatment of ASD.","PeriodicalId":15985,"journal":{"name":"Journal of genetics and genomics = Yi chuan xue bao","volume":" ","pages":"338-351"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10579789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential and progress of studying mountain biodiversity by means of butterfly genetics and genomics. 利用蝴蝶遗传学和基因组学研究山区生物多样性的潜力和进展。

Journal of genetics and genomics = Yi chuan xue bao Pub Date : 2024-03-01 Epub Date: 2023-06-10 DOI: 10.1016/j.jgg.2023.06.001

Shuting Wang, Marco Girardello, Wei Zhang

引用次数: 0

HvMPK4 phosphorylates HvWRKY1 to enhance its suppression of barley immunity to powdery mildew fungus. HvMPK4 磷酸化 HvWRKY1，增强其对大麦白粉病菌免疫力的抑制作用。

Journal of genetics and genomics = Yi chuan xue bao Pub Date : 2024-03-01 Epub Date: 2023-05-22 DOI: 10.1016/j.jgg.2023.05.005

Pengya Xue, Ling Zhang, Renchun Fan, Yanan Li, Xinyun Han, Ting Qi, Lifang Zhao, Deshui Yu, Qian-Hua Shen

{"title":"HvMPK4 phosphorylates HvWRKY1 to enhance its suppression of barley immunity to powdery mildew fungus.","authors":"Pengya Xue, Ling Zhang, Renchun Fan, Yanan Li, Xinyun Han, Ting Qi, Lifang Zhao, Deshui Yu, Qian-Hua Shen","doi":"10.1016/j.jgg.2023.05.005","DOIUrl":"10.1016/j.jgg.2023.05.005","url":null,"abstract":"Mitogen-activated protein kinase (MAPK) cascades play important roles in disease resistance in model plant species. However, the functions of MAPK signaling pathways in crop disease resistance are largely unknown. Here we report the function of HvMKK1-HvMPK4-HvWRKY1 module in barley immune system. HvMPK4 is identified to play a negative role in barley immune response against Bgh, as virus-induced gene silencing of HvMPK4 results in enhanced disease resistance whilst stably overexpressing HvMPK4 leads to super-susceptibility to Bgh infection. Furthermore, the barley MAPK kinase HvMKK1 is found to specifically interact with HvMPK4, and the activated HvMKK1DD variant specifically phosphorylates HvMPK4 in vitro. Moreover, the transcription factor HvWRKY1 is identified to be a downstream target of HvMPK4 and phosphorylated by HvMPK4 in vitro in the presence of HvMKK1DD. Phosphorylation assay coupled with mutagenesis analyses identifies S122, T284, and S347 in HvWRKY1 as the major residues phosphorylated by HvMPK4. HvWRKY1 is phosphorylated in barley at the early stages of Bgh infection, which enhances its suppression on barley immunity likely due to enhanced DNA-binding and transcriptional repression activity. Our data suggest that the HvMKK1-HvMPK4 kinase pair acts upstream of HvWRKY1 to negatively regulate barley immunity against powdery mildew.","PeriodicalId":15985,"journal":{"name":"Journal of genetics and genomics = Yi chuan xue bao","volume":" ","pages":"313-325"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10112857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein post-translational modifications in auxin signaling. 辅助素信号转导中的蛋白质翻译后修饰。

Journal of genetics and genomics = Yi chuan xue bao Pub Date : 2024-03-01 Epub Date: 2023-07-12 DOI: 10.1016/j.jgg.2023.07.002

Xiankui Cui, Junxia Wang, Ke Li, Bingsheng Lv, Bingkai Hou, Zhaojun Ding

引用次数: 0

Genetic and lipidomic analyses reveal the key role of lipid metabolism for cold tolerance in maize. 遗传和脂质体分析揭示了脂质代谢对玉米耐寒性的关键作用。

Journal of genetics and genomics = Yi chuan xue bao Pub Date : 2024-03-01 Epub Date: 2023-07-20 DOI: 10.1016/j.jgg.2023.07.004

Lei Gao, Haifang Jiang, Minze Li, Danfeng Wang, Hongtao Xiang, Rong Zeng, Limei Chen, Xiaoyan Zhang, Jianru Zuo, Shuhua Yang, Yiting Shi

{"title":"Genetic and lipidomic analyses reveal the key role of lipid metabolism for cold tolerance in maize.","authors":"Lei Gao, Haifang Jiang, Minze Li, Danfeng Wang, Hongtao Xiang, Rong Zeng, Limei Chen, Xiaoyan Zhang, Jianru Zuo, Shuhua Yang, Yiting Shi","doi":"10.1016/j.jgg.2023.07.004","DOIUrl":"10.1016/j.jgg.2023.07.004","url":null,"abstract":"Lipid remodeling is crucial for cold tolerance in plants. However, the precise alternations of lipidomics during cold responses remain elusive, especially in maize (Zea mays L.). In addition, the key genes responsible for cold tolerance in maize lipid metabolism have not been identified. Here, we integrate lipidomic, transcriptomic, and genetic analysis to determine the profile of lipid remodeling caused by cold stress. We find that the homeostasis of cellular lipid metabolism is essential for maintaining cold tolerance of maize. Also, we detect 210 lipid species belonging to 13 major classes, covering phospholipids, glycerides, glycolipids, and free fatty acids. Various lipid metabolites undergo specific and selective alterations in response to cold stress, especially mono-/di-unsaturated lysophosphatidic acid, lysophosphatidylcholine, phosphatidylcholine, and phosphatidylinositol, as well as polyunsaturated phosphatidic acid, monogalactosyldiacylglycerol, diacylglycerol, and triacylglycerol. In addition, we identify a subset of key enzymes, including ketoacyl-acyl-carrier protein synthase II (KAS II), acyl-carrier protein 2 (ACP2), male sterility33 (Ms33), and stearoyl-acyl-carrier protein desaturase 2 (SAD2) involved in glycerolipid biosynthetic pathways are positive regulators of maize cold tolerance. These results reveal a comprehensive lipidomic profile during the cold response of maize and provide genetic resources for enhancing cold tolerance in crops.","PeriodicalId":15985,"journal":{"name":"Journal of genetics and genomics = Yi chuan xue bao","volume":" ","pages":"326-337"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10065613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging roles and therapeutic potentials of sphingolipids in pathophysiology: emphasis on fatty acyl heterogeneity. 鞘磷脂在病理生理学中的新作用和治疗潜力：强调脂肪酰基的异质性。

Journal of genetics and genomics = Yi chuan xue bao Pub Date : 2024-03-01 Epub Date: 2023-06-25 DOI: 10.1016/j.jgg.2023.06.006

Jinming Mu, Sin Man Lam, Guanghou Shui

引用次数: 0

ORPA: a fast and efficient phylogenetic analysis method for constructing genome-wide alignments of organelle genomes. ORPA：一种快速有效的系统发育分析方法，用于构建细胞器基因组的全基因组比对。

Journal of genetics and genomics = Yi chuan xue bao Pub Date : 2024-03-01 Epub Date: 2023-08-25 DOI: 10.1016/j.jgg.2023.08.006

Guiqi Bi, Xinxin Luan, Jianbin Yan

引用次数: 0