Journal of general microbiology最新文献_第9页

Variation in the size of the ospA-containing linear plasmid, but not the linear chromosome, among the three Borrelia species associated with Lyme disease. 与莱姆病相关的三种疏螺旋体中含有ospa的线性质粒大小的变异，而不是线性染色体。

Journal of general microbiology Pub Date : 1993-10-01 DOI: 10.1099/00221287-139-10-2445

D S Samuels, R T Marconi, C F Garon

引用次数: 45

Construction of Bacillus anthracis mutant strains producing a single toxin component. 产生单一毒素成分的炭疽芽孢杆菌突变株的构建。

Journal of general microbiology Pub Date : 1993-10-01 DOI: 10.1099/00221287-139-10-2459

C Pezard, E Duflot, M Mock

引用次数: 68

Targeting of interleukin-2 to the periplasm of Escherichia coli. 白细胞介素-2对大肠杆菌外周质的靶向作用。

Journal of general microbiology Pub Date : 1993-10-01 DOI: 10.1099/00221287-139-10-2465

G Halfmann, H Brailly, A Bernadac, F A Montero-Julian, C Lazdunski, D Baty

{"title":"Targeting of interleukin-2 to the periplasm of Escherichia coli.","authors":"G Halfmann, H Brailly, A Bernadac, F A Montero-Julian, C Lazdunski, D Baty","doi":"10.1099/00221287-139-10-2465","DOIUrl":"https://doi.org/10.1099/00221287-139-10-2465","url":null,"abstract":"A synthetic gene coding for interleukin-2 (IL-2) was used to produce large amounts of recombinant IL-2 (met-IL-2) in Escherichia coli. Met-IL-2 was found to accumulate in the cytoplasm in an insoluble, aggregated form. Inclusion bodies located at the pole caps of cells were detected using immunogold labelling. Constructs were designed to fuse the IL-2 gene to DNA fragments encoding signal peptides for an outer-membrane protein (OmpA) or for a periplasmic protein (PhoA) of E. coli. No significant maturation was observed with these fusion proteins which were found in an insoluble form in the cytoplasm. The influence of charge disposition at the N-terminus of the mature portion of the protein was investigated by replacing positively charged amino acids with glutamic acid. None of the introduced substitutions had any effect. Various factors that might affect expression, secretion and folding were examined in an attempt to obtain secretion. By fusing IL-2 to the precursor maltose-binding protein (preMBP) a large fraction of the preMBP-IL-2 protein was correctly processed and transported to the periplasmic space. IL-2 derived from MBP-IL-2 after FXa cleavage possessed similar specific activity to recombinant IL-2 produced in Chinese Hamster ovary cells.","PeriodicalId":15884,"journal":{"name":"Journal of general microbiology","volume":"139 10","pages":"2465-73"},"PeriodicalIF":0.0,"publicationDate":"1993-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00221287-139-10-2465","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19243205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Genotypic typing and phylogenetic analysis of Salmonella paratyphi B and S. java with IS200. 副伤寒沙门氏菌B和爪哇沙门氏菌IS200基因型分型及系统发育分析。

Journal of general microbiology Pub Date : 1993-10-01 DOI: 10.1099/00221287-139-10-2409

E Ezquerra, A Burnens, C Jones, J Stanley

{"title":"Genotypic typing and phylogenetic analysis of Salmonella paratyphi B and S. java with IS200.","authors":"E Ezquerra, A Burnens, C Jones, J Stanley","doi":"10.1099/00221287-139-10-2409","DOIUrl":"https://doi.org/10.1099/00221287-139-10-2409","url":null,"abstract":"Salmonella paratyphi B and Salmonella java are biovars of common serotype 1,4,[5],12:b:1,2 which respectively cause human paratyphoid fever and gastroenteritis. In order to define genotypes and phylogenetic relationships in this group, we examined representative strains for restriction fragment length polymorphisms (RFLPs) in and around the 16S ribosomal RNA (rrn) genes, and the five to eleven insertion sites of the Salmonella-specific DNA insertion sequence IS200. One of four 16S rrn profiles was predominant, and was shared by the majority of strains, irrespective of their designation as S. paratyphi B or S. java. On the other hand, thirteen unique IS200 profiles were found and this technique was able to distinguish, for the first time, distinct genotypes for S. paratyphi B and S. java. One of the S. paratyphi B profiles, Spj-IP1.0, represented a globally-distributed clone. Greater diversity was detected within IS200 profiles of S. java than within those of S. paratyphi B. IS200 profiles described a phylogenetic complex in which strains of both biovars could be placed. They constituted reproducible molecular fingerprints, which could be compared in a band-matching database suitable for molecular epidemiological typing.","PeriodicalId":15884,"journal":{"name":"Journal of general microbiology","volume":"139 10","pages":"2409-14"},"PeriodicalIF":0.0,"publicationDate":"1993-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00221287-139-10-2409","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19242138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

fliU and fliV: two flagellar genes essential for biosynthesis of Salmonella and Escherichia coli flagella. flliu和fliV:沙门氏菌和大肠杆菌鞭毛生物合成所必需的两个鞭毛基因。

Journal of general microbiology Pub Date : 1993-10-01 DOI: 10.1099/00221287-139-10-2415

L Doll, G Frankel

引用次数: 9

Nucleic acid sequence-based amplification (NASBA) for the identification of mycobacteria. 核酸序列扩增(NASBA)技术鉴定分枝杆菌。

Journal of general microbiology Pub Date : 1993-10-01 DOI: 10.1099/00221287-139-10-2423

G M van der Vliet, R A Schukkink, B van Gemen, P Schepers, P R Klatser

{"title":"Nucleic acid sequence-based amplification (NASBA) for the identification of mycobacteria.","authors":"G M van der Vliet, R A Schukkink, B van Gemen, P Schepers, P R Klatser","doi":"10.1099/00221287-139-10-2423","DOIUrl":"https://doi.org/10.1099/00221287-139-10-2423","url":null,"abstract":"Nucleic acid sequence-based amplification (NASBA), an isothermal amplification technique for nucleic acids (NA), was investigated for the species-specific identification of mycobacteria. A set of primers was selected from a highly conserved region of the 16S rRNA sequence of mycobacteria sandwiching a variable sequence to perform amplification of mycobacterial RNA. Species-specific probes for the M. tuberculosis complex, M. avium-paratuberculosis, M. intracellulare and M. leprae were hybridized in-solution with the amplified nucleic acids of 10 pathogenic mycobacteria and 11 closely related bacteria, as well as with human-derived NA in an enzyme-linked gel assay (ELGA). Each probe was shown to hybridize specifically to the amplified single-stranded RNA of the corresponding species. Thirty-two clinical isolates of M. tuberculosis strains from different parts of the world were correctly identified by NASBA using the M. tuberculosis-complex-specific probe. In combination with the ELGA, NASBA could identify mycobacteria rapidly, i.e. in less than 6 h. The relative simplicity and rapidity of this technique makes it an attractive tool for species-specific identification of mycobacteria.","PeriodicalId":15884,"journal":{"name":"Journal of general microbiology","volume":"139 10","pages":"2423-9"},"PeriodicalIF":0.0,"publicationDate":"1993-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00221287-139-10-2423","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19242140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 126

Transformation of Helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker. 用甲硝唑染色体抗性和带选择性氯霉素抗性标记的质粒转化幽门螺杆菌。

Journal of general microbiology Pub Date : 1993-10-01 DOI: 10.1099/00221287-139-10-2485

Y Wang, K P Roos, D E Taylor

{"title":"Transformation of Helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker.","authors":"Y Wang, K P Roos, D E Taylor","doi":"10.1099/00221287-139-10-2485","DOIUrl":"https://doi.org/10.1099/00221287-139-10-2485","url":null,"abstract":"Most strains of Helicobacter pylori are naturally competent for uptake of chromosomal DNA. Transformation frequencies for streptomycin resistance or rifampicin resistance markers ranged from 1 x 10(-4) to 1 x 10(-3) per viable cell using a plate transformation procedure. Transformation of a metronidazole resistance marker (MtrR) was demonstrated when either a laboratory-derived mutant or a MtrR clinical isolate were used as the source of donor DNA. MtrR was transformed at a frequency of 3 x 10(-5) per viable cell. All H. pylori strains tested produce large amounts of DNAase, which may reduce DNA available for transformation. Four H. pylori plasmids were isolated. DNA fragments from H. pylori plasmids were deleted or rearranged when cloned in pUC19 and propagated in Escherichia coli DH5 alpha. An H. pylori plasmid, pUOA26 which contained a chloramphenicol resistance determinant from Campylobacter coli, was constructed in H. pylori. This plasmid could be successfully introduced by natural transformation only into H. pylori recipients which contained a homologous resident plasmid. Transformation of pUOA26 into plasmid-free cells of H. pylori was achieved by electroporation. Transformation frequencies were 1 x 10(-4) transformants per viable cell when plasmid DNA was isolated from the same strain; however, introduction of pUOA26 DNA derived from H. pylori 8091 into a different H. pylori strain, NCTC 11639, resulted in transformation at much lower frequencies (< or = 1 x 10(-7) per viable cell).(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":15884,"journal":{"name":"Journal of general microbiology","volume":"139 10","pages":"2485-93"},"PeriodicalIF":0.0,"publicationDate":"1993-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00221287-139-10-2485","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19243207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 180

Analysis of the Anaplasma marginale genome by pulsed-field electrophoresis. 边缘无原体基因组的脉冲场电泳分析。

Journal of general microbiology Pub Date : 1993-10-01 DOI: 10.1099/00221287-139-10-2439

A R Alleman, S M Kamper, N Viseshakul, A F Barbet

{"title":"Analysis of the Anaplasma marginale genome by pulsed-field electrophoresis.","authors":"A R Alleman, S M Kamper, N Viseshakul, A F Barbet","doi":"10.1099/00221287-139-10-2439","DOIUrl":"https://doi.org/10.1099/00221287-139-10-2439","url":null,"abstract":"Anaplasma marginale is a rickettsial parasite of bovine erythrocytes causing world-wide economic losses in livestock production. Despite its importance, little is known about this rickettsia at a molecular level because it has not been cultured in vitro, and there is no small-animal model. Although several genes have been cloned and sequenced, the gross genome structure of the organism has not yet been well characterized. We separated intact bovine erythrocytes from leucocytes, and determined the genome size of A. marginale by use of restriction endonuclease cleavage and pulsed-field gel electrophoresis (PFGE). A value of 56 mol% G+C was obtained for this genome by spectral analysis. Undigested A. marginale DNA failed to migrate under several different electrophoretic conditions, indicating a circular genome. Digestions of intact A. marginale DNA were performed using restriction endonucleases NotI, SfiI and PacI. Complete digestion with SfiI resulted in 12 distinct bands ranging in size from 14 to 170 kbp. Total size determined by addition of SfiI-digested fragments was approximately 1200 kbp. PacI cleaved the A. marginale genome from three different isolates into just three fragments, of 598, 557 and 97 kbp. Incomplete digestion produced a band measuring 1250 kbp. These results indicate that A. marginale has a circular genome between 1200 and 1260 kbp, with a G+C content of 56 mol%.","PeriodicalId":15884,"journal":{"name":"Journal of general microbiology","volume":"139 10","pages":"2439-44"},"PeriodicalIF":0.0,"publicationDate":"1993-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00221287-139-10-2439","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18900088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

A haemagglutinating adhesin of group B streptococci isolated from cases of bovine mastitis mediates adherence to HeLa cells. 从牛乳腺炎病例中分离的B组链球菌血凝黏附素介导对HeLa细胞的粘附。

Journal of general microbiology Pub Date : 1993-09-01 DOI: 10.1099/00221287-139-9-2173

I W Wibawan, C Lämmler, R S Seleim, F H Pasaribu

{"title":"A haemagglutinating adhesin of group B streptococci isolated from cases of bovine mastitis mediates adherence to HeLa cells.","authors":"I W Wibawan, C Lämmler, R S Seleim, F H Pasaribu","doi":"10.1099/00221287-139-9-2173","DOIUrl":"https://doi.org/10.1099/00221287-139-9-2173","url":null,"abstract":"Rabbit erythrocytes were agglutinated by 43.4% of group B streptococci isolated from bovines but by none isolated from humans. Haemagglutination was enhanced by cultivation of the bacteria under microaerophilic conditions. Most of the haemagglutinating strains had protein type antigen X, either alone, or in combination with polysaccharide antigens. Heat and proteolytic treatment of the bacteria destroyed the haemagglutination activity. The haemagglutinin could be solubilized from the bacterial surface by mutanolysin treatment and isolated from culture supernatant fluid by ammonium sulphate precipitation. The isolated haemagglutinin did not cause direct agglutination of erythrocytes. However, binding of the haemagglutinin to rabbit erythrocytes could be visualized by agglutination of haemagglutinin-treated erythrocytes by specific antiserum obtained by absorption. Western blotting showed that the haemagglutinin obtained from erythrocyte lysates contained an antibody-reactive band with a molecular mass of 43 kDa. Haemagglutination-positive strains adhered to HeLa cells in higher numbers than did haemagglutination-negative strains. The HeLa cell adherence of Group B streptococci was inhibited in the presence of isolated haemagglutinin or of specific antiserum against the haemagglutinin. These observations suggest that the haemagglutinating adhesins of bovine group B streptococcal isolates are directly involved in the adherence mechanisms of these organisms.","PeriodicalId":15884,"journal":{"name":"Journal of general microbiology","volume":"139 9","pages":"2173-8"},"PeriodicalIF":0.0,"publicationDate":"1993-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00221287-139-9-2173","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19235250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Detection of the outer membrane lipoprotein I and its gene in fluorescent and non-fluorescent pseudomonads: implications for taxonomy and diagnosis. 荧光和非荧光假单胞菌外膜脂蛋白I及其基因的检测:对分类和诊断的意义。

Journal of general microbiology Pub Date : 1993-09-01 DOI: 10.1099/00221287-139-9-2215

D De Vos, A Lim, P De Vos, A Sarniguet, K Kersters, P Cornelis

引用次数: 42