Journal of general microbiology最新文献_第7页

Genetic structure of Neisseria gonorrhoeae populations: a non-clonal pathogen. 淋病奈瑟菌群体的遗传结构:一种非克隆病原体。

Journal of general microbiology Pub Date : 1993-11-01 DOI: 10.1099/00221287-139-11-2603

M O'Rourke, E Stevens

引用次数: 104

Purification and characterization of glucose-6-phosphate dehydrogenase from Aspergillus niger and Aspergillus nidulans. 黑曲霉和中性曲霉中葡萄糖-6-磷酸脱氢酶的纯化及特性研究。

Journal of general microbiology Pub Date : 1993-11-01 DOI: 10.1099/00221287-139-11-2793

L M Wennekes, T Goosen, P J van den Broek, H W van den Broek

引用次数: 22

Diversity of Bacillus thuringiensis environmental isolates showing larvicidal activity specific for mosquitoes. 苏云金芽孢杆菌环境分离株对蚊虫具有特异性杀幼虫活性的多样性。

Journal of general microbiology Pub Date : 1993-11-01 DOI: 10.1099/00221287-139-11-2849

T Ishii, M Ohba

{"title":"Diversity of Bacillus thuringiensis environmental isolates showing larvicidal activity specific for mosquitoes.","authors":"T Ishii, M Ohba","doi":"10.1099/00221287-139-11-2849","DOIUrl":"https://doi.org/10.1099/00221287-139-11-2849","url":null,"abstract":"Seven mosquito-specific strains of Bacillus thuringiensis isolated in Japan were examined for their flagellar (H) antigenicities and the properties of parasporal inclusion proteins. They were assigned to six H serovars: serovar fukuokaensis (H 3ade); serovar canadensis (H 5ac); serovar darmstadiensis (H 10); serovar kyushuensis (H 11ac); serovar shandongiensis (H 22); and an undescribed serovar belonging to H serotype 20. Purified parasporal inclusions exhibited moderate mosquito larvicidal activities with LC50 values ranging from 1 microgram ml-1 to 10 micrograms ml-1. The inclusions of these strains consisted of highly heterogeneous multiple protein components, and five distinct patterns were evident in their SDS-PAGE profiles. Antibodies against kyushuensis inclusion proteins were reactive with all strains to varying degrees, while israelensis antibodies gave only relatively weak reactions with the seven strains. Similarity in antibody-binding profiles was associated with similarity in SDS-PAGE profiles. In a given strain, different antisera gave altered immunoblot profiles. Haemolytic activity was shown by solubilized parasporal inclusion proteins of five of the seven strains.","PeriodicalId":15884,"journal":{"name":"Journal of general microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00221287-139-11-2849","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18901263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49

The draTG gene region of Rhodobacter capsulatus is required for post-translational regulation of both the molybdenum and the alternative nitrogenase. 荚膜红杆菌(Rhodobacter capsulatus)的draTG基因区域对钼酶和替代氮酶的翻译后调控都是必需的。

Journal of general microbiology Pub Date : 1993-11-01 DOI: 10.1099/00221287-139-11-2667

B Masepohl, R Krey, W Klipp

{"title":"The draTG gene region of Rhodobacter capsulatus is required for post-translational regulation of both the molybdenum and the alternative nitrogenase.","authors":"B Masepohl, R Krey, W Klipp","doi":"10.1099/00221287-139-11-2667","DOIUrl":"https://doi.org/10.1099/00221287-139-11-2667","url":null,"abstract":"Synthetic oligonucleotides, which were designed according to amino acid sequences conserved between Rhodospirillum rubrum and Azospirillum brasilense DraT and DraG, respectively, were used to identify the corresponding genes of Rhodobacter capsulatus. Sequence analysis of a 1904 bp DNA fragment proved the existence of R. capsulatus draT and draG. These two genes were separated by 11 bp only, suggesting that R. capsulatus draT and draG were part of one transcriptional unit. In contrast to R. rubrum, A. brasilense and Azospirillum lipoferum, the R. capsulatus draTG genes were not located upstream of the structural genes of nitrogenase nifHDK but close to the dctP gene at a distance of about 1000 kb from the nifHDK genes. Deletion mutations in the draTG gene region were constructed and introduced into R. capsulatus wild-type and a nifHDK deletion strain. The resulting mutant strains were examined for post-translational regulation of the molybdenum and the alternative nitrogenase in response to ammonia and darkness. Under 'switch-off' conditions the modified (ADP-ribosylated) and the non-modified forms of component II of both the molybdenum and the alternative nitrogenase were detected in a draTG wild-type background by immunoblot analysis, whereas only the non-modified forms were present in the draTG deletion strains. Nitrogenase activity in these strains was followed by the acetylene reduction assay. In contrast to the wild-type, draTG mutants were not affected in nitrogenase activity in response to ammonia or darkness. These results demonstrated that the draTG genes are required for post-translational regulation of both the molybdenum and the heterometal-free nitrogenase in R. capsulatus.","PeriodicalId":15884,"journal":{"name":"Journal of general microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00221287-139-11-2667","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19264542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 77

Stimulation of genetic instability in Streptomyces ambofaciens ATCC 23877 by antibiotics that interact with DNA gyrase. 与DNA旋切酶相互作用的抗生素对双歧链霉菌ATCC 23877遗传不稳定性的刺激

Journal of general microbiology Pub Date : 1993-11-01 DOI: 10.1099/00221287-139-11-2551

J N Volff, D Vandewiele, J M Simonet, B Decaris

{"title":"Stimulation of genetic instability in Streptomyces ambofaciens ATCC 23877 by antibiotics that interact with DNA gyrase.","authors":"J N Volff, D Vandewiele, J M Simonet, B Decaris","doi":"10.1099/00221287-139-11-2551","DOIUrl":"https://doi.org/10.1099/00221287-139-11-2551","url":null,"abstract":"In wild-type Streptomyces ambofaciens ATCC 23877, pigment-defective (Pig-) mutants arise at a frequency of about 0.5%; this genetic instability is related to genomic rearrangements such as deletions and/or amplifications of DNA sequences. On media containing oxolinic acid and novobiocin, which interact with the A and B subunits of DNA gyrase, respectively, the frequency of variants increased dramatically. The Pig- mutant frequency was increased to almost 100% on a medium containing oxolinic acid at a concentration allowing 55% survival. On solid medium containing either oxolinic acid or novobiocin at subinhibitory concentrations, most colonies exhibited a 'patchwork' phenotype, characterized by the presence of numerous Pig- sectors. Similar phenomena were not observed on media containing the transcriptional inhibitor rifampicin or the translational inhibitor streptomycin. Many of the Pig- mutants exhibited a pleiotropic phenotype and were affected in aerial mycelium formation, colony growth and/or prototrophy. Moreover, the same kinds of rearrangements (deletions and/or amplifications of DNA sequences) were found in both induced and spontaneous Pig- mutants. The results suggest either that DNA gyrase is directly involved in genetic instability or that an SOS-like system is implicated.","PeriodicalId":15884,"journal":{"name":"Journal of general microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00221287-139-11-2551","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19265277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Albumin-binding proteins on the surface of the Streptococcus milleri group and characterization of the albumin receptor of Streptococcus intermedius C5. 米勒氏链球菌群表面白蛋白结合蛋白及中间链球菌C5白蛋白受体的表征。

Journal of general microbiology Pub Date : 1993-10-01 DOI: 10.1099/00221287-139-10-2451

M D Willcox, M Patrikakis, C Y Loo, K W Knox

引用次数: 18

Proteolysis and orientation in Dictyostelium slugs. 盘齿钢门蛞蝓的蛋白质水解与取向。

Journal of general microbiology Pub Date : 1993-10-01 DOI: 10.1099/00221287-139-10-2319

J T Bonner

引用次数: 15

The distribution of the outer gas vesicle protein, GvpC, on the Anabaena gas vesicle, and its ratio to GvpA. 外囊泡蛋白GvpC在水藻囊泡上的分布及其与GvpA的比值。

Journal of general microbiology Pub Date : 1993-10-01 DOI: 10.1099/00221287-139-10-2353

B E Buchholz, P K Hayes, A E Walsby

{"title":"The distribution of the outer gas vesicle protein, GvpC, on the Anabaena gas vesicle, and its ratio to GvpA.","authors":"B E Buchholz, P K Hayes, A E Walsby","doi":"10.1099/00221287-139-10-2353","DOIUrl":"https://doi.org/10.1099/00221287-139-10-2353","url":null,"abstract":"Previous studies have shown that gas vesicles isolated from the cyanobacterium Anabaena flos-aquae contain two types of protein, GvpA, a small hydrophobic protein that forms the main ribbed structure, and GvpC, a protein comprising five repeats of a 33-amino-acid-residue motif, which is located on the outer surface of the GvpA shell. GvpC was shown to increase the critical collapse pressure of the gas vesicles; it was thought to do this by forming a series of molecular ties that bind the ribs together. We now show that antibodies raised against GvpC label both the central cylinders and the conical end caps of native gas vesicles but fail to bind to gas vesicles that have been stripped of GvpC. The molar ratio of GvpA to GvpC has been calculated from amino acid analyses of gas vesicle hydrolysates by reference to the abundance of amino acids that occur predominantly or exclusively in one protein or the other; the molar ratio was found to be 25:1 in freshly isolated gas vesicles and 23:1 in gas vesicles saturated with GvpC. We have considered three ways in which the 33-residue repeats of GvpC might interact with the crystallographic unit cell of GvpA molecules in the ribs. The Anabaena GvpC will bind to and restore the strength of gas vesicles isolated from Aphanizomenon and Microcystis that lack their native GvpC.","PeriodicalId":15884,"journal":{"name":"Journal of general microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00221287-139-10-2353","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19242818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

Cloning, nucleotide sequence and characterization of the mannitol dehydrogenase gene from Rhodobacter sphaeroides. 球形红杆菌甘露醇脱氢酶基因的克隆、核苷酸序列及特性分析。

Journal of general microbiology Pub Date : 1993-10-01 DOI: 10.1099/00221287-139-10-2475

K H Schneider, F Giffhorn, S Kaplan

{"title":"Cloning, nucleotide sequence and characterization of the mannitol dehydrogenase gene from Rhodobacter sphaeroides.","authors":"K H Schneider, F Giffhorn, S Kaplan","doi":"10.1099/00221287-139-10-2475","DOIUrl":"https://doi.org/10.1099/00221287-139-10-2475","url":null,"abstract":"Transposon mutagenesis and antibiotic enrichment were employed to isolate a mutant of Rhodobacter sphaeroides Si4 designated strain M22, that had lost the ability to grow on D-mannitol and to produce the enzyme mannitol dehydrogenase (MDH). DNA flanking the transposon in the mutant strain was used as a probe for the identification and cloning of the MDH gene (mtlK). A 5.5 kb EcoRI/BglII fragment from R. sphaeroides Si4 was isolated and shown to complement the mutation in R. sphaeroides M22. Successful complementation required that a promoter of the vector-plasmid pRK415 be present, suggesting that the mtlK gene is part of a larger operon. Using oligonucleotides derived from the N-terminal sequence of MDH as probes mtlK was located on the complementing fragment and the gene was sequenced. The mtlK open reading frame encodes a protein of 51,404 Da with an N-terminal sequence identical to that obtained from amino acid analysis of the purified MDH. The MDH of R. sphaeroides Si4 exhibits distant similarity to the mannitol-1-phosphate dehydrogenases from Escherichia coli and Enterococcus faecalis, with 28.1% and 26.3% identity, respectively. Mutant strains deficient in MtlK displayed substantial levels of sorbitol dehydrogenase activity, originally thought to be only a minor activity associated with the MDH enzyme. It is likely that we have uncovered an additional polyol dehydrogenase with activity for sorbitol. The mtlK gene can be used for overexpression of MDH in E. coli in order to obtain sufficient amounts of enzyme for further investigations and applications.","PeriodicalId":15884,"journal":{"name":"Journal of general microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00221287-139-10-2475","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19243206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47

Helicobacter canis sp. nov., a new species from dogs: an integrated study of phenotype and genotype. 犬类新种犬幽门螺杆菌:表型与基因型的综合研究。

Journal of general microbiology Pub Date : 1993-10-01 DOI: 10.1099/00221287-139-10-2495

J Stanley, D Linton, A P Burnens, F E Dewhirst, R J Owen, A Porter, S L On, M Costas

{"title":"Helicobacter canis sp. nov., a new species from dogs: an integrated study of phenotype and genotype.","authors":"J Stanley, D Linton, A P Burnens, F E Dewhirst, R J Owen, A Porter, S L On, M Costas","doi":"10.1099/00221287-139-10-2495","DOIUrl":"https://doi.org/10.1099/00221287-139-10-2495","url":null,"abstract":"A group of Campylobacter-like organisms (CLOs) were isolated from the faeces of diarrhoeic or healthy dogs, constituting 4% of all CLOs from this source. Since they formed a unique DNA homology group within the genus Helicobacter, and exhibited distinctive phenotypic properties, they were collectively termed the HC group. A polyphasic taxonomic analysis was made of this group. The phenotype of four dog isolates and a single human isolate was unique and could be distinguished bacteriologically from other helicobacters. Electron microscopic ultrastructure revealed defining characteristics of Helicobacter. The 16S rRNA gene of the nominated type strain NCTC 12739T was sequenced, and its analysis delineated the group as a new species of Helicobacter. This conclusion was supported by relative DNA homology and whole-cell protein electrophoretic patterns. We therefore propose the name Helicobacter canis sp. nov. for this group. The species most closely related to H. canis sp. nov. were H. cinaedi, 'Flexispira rappini' and H. fennelliae. A species-specific recombinant DNA probe was cloned from NCTC 12739T for use in routine laboratory identification and epidemiological studies. The faecal source, bile tolerance and lack of urease activity of H. canis sp. nov. suggest that this new Helicobacter species colonizes the lower bowel rather than the stomach.","PeriodicalId":15884,"journal":{"name":"Journal of general microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00221287-139-10-2495","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19243208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 159