{"title":"Complete Genome Sequence of <i>Annamia dubia</i>, filamentous colony-making Chroococcales with the analysis of FraC gene influencing filament integrity.","authors":"Akihiro Tuji, Eri Ogiso-Tanaka, Haruyo Yamaguchi","doi":"10.7150/jgen.87678","DOIUrl":"10.7150/jgen.87678","url":null,"abstract":"<p><p>The complete genome of <i>Annamia dubia</i> was sequenced. The genome size is 4.02 Mbp, including 3886286 bp circular chromosome and four circular plasmids (31516, 42453, 38085 and 24903 bp). It included 3718 protein-coding sequences, 45 tRNA genes, three sets of rRNA genes, a microcystin biosynthesis gene cluster and six CRISPR (clustered regularly interspaced short palindromic repeat). <i>Annamia</i> is the only one genus in the Chroococcales that makes filamentous colonies. FraC and FraG were identified in the genome. These genes are required for the integrity of cell junctions and influencing filament integrity and are thought to be related to colony formation. These genes are first reported from Chroococcales, and may play a significant role in the colony formation of this species. In the phylogenetic tree of the FraC gene, <i>A. dubia</i> was located in the basal position of Oscillatoriales. The GC ratio of FraC gene of <i>A. dubia</i> is very low from the genome and the FraC gene of Microcoleaceae. The presence of these genes in the basal region and the low GC ratio suggests that the FraC gene in this species was introduced by horizontal gene transfer. Since the filamentous colony is a fundamental and important taxonomic feature for the classification of cyanobacteria, the possibility of horizontal transmission of genes involved in filamentous cyanobacterial colonies is an important discovery for the classification of cyanobacteria.</p>","PeriodicalId":15834,"journal":{"name":"Journal of Genomics","volume":"12 ","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10751749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139074277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saroja Chhettri, Joseph Sevigny, Céline Pesce, Indrani Sarkar, W Kelley Thomas, Imen Nouioui, Gargi Sen, Louis S Tisa, Arnab Sen
{"title":"Whole genome sequencing of <i>Streptomyces antnestii</i> sp. nov. with a potency to become an industrial strain.","authors":"Saroja Chhettri, Joseph Sevigny, Céline Pesce, Indrani Sarkar, W Kelley Thomas, Imen Nouioui, Gargi Sen, Louis S Tisa, Arnab Sen","doi":"10.7150/jgen.87156","DOIUrl":"10.7150/jgen.87156","url":null,"abstract":"<p><p><i>Streptomyces</i> Strain San01 is isolated from the soil of ant-nest found in the tea estate of Darjeeling, India. The morphology, biochemical, as well as the molecular characteristics, proved that San01 belonged to the genus <i>Streptomyces.</i> The average nucleotide identity (ANI) value between the genome sequence of the studied strain and its closest phylogenetic neighbors were very low and also could be distinguished from its closest neighbour with broad range of phenotypic data. The draft genome sequence of isolate San01 (NZ_RZYA00000000.1) was estimated to be 9.12 Mbp in size with 71.2% of GC content and it encompasses 39 biosynthetic gene clusters that emphasize the biotechnological potential of this isolate<i>.</i>Based on the phenotypic, genetic and genomic data, isolate San01 (=JCM 34633 = NCTC 14543) merits to be recognized as a type strain of a novel species and hereby propose the name <i>Streptomyces antnestii</i> sp. nov. Incidentally, this is the first report on <i>Streptomyces</i> genomes from Darjeeling, India.</p>","PeriodicalId":15834,"journal":{"name":"Journal of Genomics","volume":"12 ","pages":"6-13"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10751750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139074279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Heike Möller, Beatrix Coetzee, Jan van Niekerk, Lindy Joy Rose
{"title":"Reference Genome Resource for the Citrus Pathogen <i>Phytophthora citrophthora</i>.","authors":"Heike Möller, Beatrix Coetzee, Jan van Niekerk, Lindy Joy Rose","doi":"10.7150/jgen.89324","DOIUrl":"10.7150/jgen.89324","url":null,"abstract":"<p><p><i>Phytophthora citrophthora</i> is an oomycete pathogen that infects citrus. Its occurrence in citrus-growing regions worldwide is considered a major contributor to crop losses. This study presents a high-quality genome resource for <i>P. citrophthora</i>, which was generated using PacBio HiFi long-read high-throughput sequencing technology. We successfully assembled a 48.5 Mb genome containing 16,409 protein-coding genes from high-quality reads. This marks the first complete genome assembly of <i>P. citrophthora</i>, providing a valuable resource to enhance the understanding of pathogenic behaviour and fungicide sensitivity of this destructive citrus pathogen.</p>","PeriodicalId":15834,"journal":{"name":"Journal of Genomics","volume":"12 ","pages":"14-18"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10751748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139074278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of GenomicsPub Date : 2023-11-01eCollection Date: 2023-01-01DOI: 10.7150/jgen.88302
Diariatou Niang, Abdellatif Gueddou, Nogaye Niang, Darius Nzepang, Aissatou Sambou, Adama Diouf, Arlette Z Zaiya, Maimouna Cissoko, Djamel Gully, Joel-Romaric Nguepjop, Sergio Svistoonoff, Daniel Fonceka, Valérie Hocher, Diégane Diouf, Saliou Fall, Louis S Tisa
{"title":"Permanent draft genome sequence of <i>Bradyrhizobium vignae,</i> strain ISRA 400, an elite nitrogen-fixing bacterium, isolated from the groundnut growing area in Senegal.","authors":"Diariatou Niang, Abdellatif Gueddou, Nogaye Niang, Darius Nzepang, Aissatou Sambou, Adama Diouf, Arlette Z Zaiya, Maimouna Cissoko, Djamel Gully, Joel-Romaric Nguepjop, Sergio Svistoonoff, Daniel Fonceka, Valérie Hocher, Diégane Diouf, Saliou Fall, Louis S Tisa","doi":"10.7150/jgen.88302","DOIUrl":"https://doi.org/10.7150/jgen.88302","url":null,"abstract":"<p><p>A new <i>Bradyrhizobium vignae</i> strain called ISRA400 was isolated from groundnut (<i>Arachis hypogaea</i> L.) root nodules obtained by trapping the bacteria from soil samples collected in the Senegalese groundnut basin. In this study, we present the draft genome sequence of this strain ISRA400, which spans approximatively 7.9 Mbp and exhibits a G+C content of 63.4%. The genome analysis revealed the presence of 48 tRNA genes and one rRNA operon (16S, 23S, and 5S). The nodulation test revealed that this strain ISRA400 significantly improves the nodulation parameters and chlorophyll content of the <i>Arachis hypogaea</i> variety Fleur11. These findings suggest the potential of <i>Bradyrhizobium vignae</i> strain ISRA400 as an effective symbiotic partner for improving the growth and productivity of groundnut crop.</p>","PeriodicalId":15834,"journal":{"name":"Journal of Genomics","volume":"11 ","pages":"52-57"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10615618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71424077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of GenomicsPub Date : 2023-09-25eCollection Date: 2023-01-01DOI: 10.7150/jgen.87229
Hironaga Akita, Yoshiki Shinto, Zen-Ichiro Kimura
{"title":"Isolation, draft genome sequence, and identification of <i>Paenibacillus glycanilyticus</i> subsp. <i>hiroshimensis</i> CCS26.","authors":"Hironaga Akita, Yoshiki Shinto, Zen-Ichiro Kimura","doi":"10.7150/jgen.87229","DOIUrl":"https://doi.org/10.7150/jgen.87229","url":null,"abstract":"<p><p>To isolate the useful strain for fermentation to produce bioactive compounds, we screened oligotrophic bacteria, and then strain CCS26 was isolated from leaf soil collected in Japan. This strain was capable of growth on low-nutrient medium. To elucidate the taxonomy of strain CCS26, we determined the 16S rRNA gene and draft genome sequences, respectively. A phylogenetic tree based on 16S rRNA gene sequences showed that strain CCS26 clustered with <i>Paenibacillus</i> species. The draft genome sequence of strain CCS26 consisted of a total of 90 contigs containing 6,957,994 bp, with a GC content of 50.8% and comprising 6,343 predicted coding sequences. Based on analysis of the average nucleotide identity with the draft genome sequence, the strain was identified as <i>P. glycanilyticus</i> subsp. <i>hiroshimensis</i> CCS26.</p>","PeriodicalId":15834,"journal":{"name":"Journal of Genomics","volume":"11 ","pages":"48-51"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10615617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71424076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of GenomicsPub Date : 2023-09-11eCollection Date: 2023-01-01DOI: 10.7150/jgen.87228
Hironaga Akita, Yoshiki Shinto, Zen-Ichiro Kimura
{"title":"Isolation and draft genome sequence of <i>Paenibacillus</i> sp<i>.</i> CCS19.","authors":"Hironaga Akita, Yoshiki Shinto, Zen-Ichiro Kimura","doi":"10.7150/jgen.87228","DOIUrl":"https://doi.org/10.7150/jgen.87228","url":null,"abstract":"<p><p>Here, we describe the isolation and draft genome sequence of <i>Paenibacillus</i> sp. CCS19. <i>Paenibacillus</i> sp. CCS19 was isolated from leaf soil collected in Japan and identified based on similarity of the 16S rRNA sequence with related <i>Paenibacillus</i> type strains. The draft genome sequence of <i>Paenibacillus</i> sp. CCS19 consisted of a total of 107 contigs containing 6,816,589 bp, with a GC content of 51.5% and comprising 5,935 predicted coding sequences.</p>","PeriodicalId":15834,"journal":{"name":"Journal of Genomics","volume":"11 ","pages":"45-47"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10539633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41162926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of GenomicsPub Date : 2023-08-19eCollection Date: 2023-01-01DOI: 10.7150/jgen.86558
Bernard J Wolff, Jessica L Waller, Alvaro J Benitez, Anna Gaines, Andrew B Conley, Lavanya Rishishwar, Aroon T Chande, Shatavia S Morrison, I King Jordan, Maureen H Diaz, Jonas M Winchell
{"title":"Genomic analysis of <i>Chlamydia psittaci</i> from a multistate zoonotic outbreak in two chicken processing plants.","authors":"Bernard J Wolff, Jessica L Waller, Alvaro J Benitez, Anna Gaines, Andrew B Conley, Lavanya Rishishwar, Aroon T Chande, Shatavia S Morrison, I King Jordan, Maureen H Diaz, Jonas M Winchell","doi":"10.7150/jgen.86558","DOIUrl":"10.7150/jgen.86558","url":null,"abstract":"<p><p>Four <i>Chlamydia psittaci</i> isolates were recovered from clinical specimens from ill workers during a multistate outbreak at two chicken processing plants. Whole genome sequencing analyses revealed high similarity to <i>C. psittaci</i> genotype D. The isolates differed from each other by only two single nucleotide polymorphisms, indicating a common source.</p>","PeriodicalId":15834,"journal":{"name":"Journal of Genomics","volume":"11 ","pages":"40-44"},"PeriodicalIF":0.0,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10168371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of GenomicsPub Date : 2023-07-16eCollection Date: 2023-01-01DOI: 10.7150/jgen.83521
Patthanasak Rungsirivanich, Elvina Parlindungan, Jennifer Mahony, Ian O'Neill, Brian McDonnell, Francesca Bottacini, Witsanu Supandee, Narumol Thongwai, Douwe van Sinderen
{"title":"Complete Genome Sequence of <i>Floricoccus penangensis</i> ML061-4 Isolated from Assam Tea Leaf [<i>Camellia sinensis</i> var. <i>assamica</i> (J.W.Mast.) Kitam.].","authors":"Patthanasak Rungsirivanich, Elvina Parlindungan, Jennifer Mahony, Ian O'Neill, Brian McDonnell, Francesca Bottacini, Witsanu Supandee, Narumol Thongwai, Douwe van Sinderen","doi":"10.7150/jgen.83521","DOIUrl":"10.7150/jgen.83521","url":null,"abstract":"<p><p><i>Floricoccus penangensis</i> is a Gram-positive coccoid organism that is a member of the lactic acid bacteria. <i>F. penangensis</i> ML061-4 was originally isolated from the surface of an Assam tea leaf, and its genome is herein shown to contain gene clusters predicted to be involved in complex carbohydrate metabolism and biosynthesis of secondary metabolites.</p>","PeriodicalId":15834,"journal":{"name":"Journal of Genomics","volume":"11 ","pages":"37-39"},"PeriodicalIF":0.0,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10367601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9937115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Draft Genome Sequences of <i>Synechococcus</i> sp. strains CCAP1479/9, CCAP1479/10, CCAP1479/13, CCY0621, and CCY9618: Five Freshwater <i>Syn/Pro</i> Clade Picocyanobacteria.","authors":"Elliot Druce, Michele Grego, Henk Bolhuis, Penny J Johnes, Patricia Sánchez-Baracaldo","doi":"10.7150/jgen.81013","DOIUrl":"10.7150/jgen.81013","url":null,"abstract":"<p><p>Picocyanobacteria are essential primary producers in freshwaters yet little is known about their genomic diversity and ecological niches. We report here five draft genomes of freshwater picocyanobacteria: <i>Synechococcus</i> sp. CCAP1479/9, <i>Synechococcus</i> sp. CCAP1479/10, and <i>Synechococcus</i> sp. CCAP1479/13 isolated from Lake Windermere in the Lake District, UK; and <i>Synechococcus</i> sp. CCY0621 and <i>Synechococcus</i> sp. CCY9618 isolated from lakes in The Netherlands. Phylogenetic analysis reveals all five strains belonging to sub-cluster 5.2 of the <i>Synechococcus</i> and <i>Prochlorococcus</i> clade of Cyanobacteria. These five strains are divergent from <i>Synechococcus elongatus</i>, an often-used model for freshwater <i>Synechococcus</i>. Functional annotation revealed significant differences in the number of genes involved in the transport and metabolism of several macro-molecules between freshwater picocyanobacteria from sub-cluster 5.2 and <i>Synechococcus elongatus</i>, including amino acids, lipids, and carbohydrates. Comparative genomic analysis identified further differences in the presence of photosynthesis-associated proteins while gene neighbourhood comparisons revealed alternative structures of the nitrate assimilation operon <i>nirA</i>.</p>","PeriodicalId":15834,"journal":{"name":"Journal of Genomics","volume":"11 ","pages":"26-36"},"PeriodicalIF":0.0,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10161378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9423576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Journal of GenomicsPub Date : 2023-04-10eCollection Date: 2023-01-01DOI: 10.7150/jgen.82493
Jakub Lach, Dominik Strapagiel, Agnieszka Matera-Witkiewicz, Paweł Stączek
{"title":"Draft genomes of halophilic Archaea strains isolated from brines of the Carpathian Foreland, Poland.","authors":"Jakub Lach, Dominik Strapagiel, Agnieszka Matera-Witkiewicz, Paweł Stączek","doi":"10.7150/jgen.82493","DOIUrl":"10.7150/jgen.82493","url":null,"abstract":"<p><p>Halophilic Archaea are a unique group of microorganisms living in saline environments. They constitute a complex group whose biodiversity has not been thoroughly studied. Here, we report three draft genomes of halophilic Archaea isolated from brines, representing the genera of <i>Halorubrum</i>, <i>Halopenitus,</i> and <i>Haloarcula</i>. Two of these strains, Boch-26 and POP-27, were identified as members of the genera <i>Halorubrum</i> and <i>Halopenitus</i>, respectively. However, they could not be assigned to any known species because of the excessive difference in genome sequences between these strains and any other described genomes. In contrast, the third strain, Boch-26, was identified as <i>Haloarcula hispanica</i>. Genome lengths of these isolates ranged from 2.7 Mbp to 3.0 Mbp, and GC content was in the 63.77%-68.77% range. Moreover, functional analysis revealed biosynthetic gene clusters (BGCs) related to terpenes production in all analysed genomes and one BGC for RRE (RiPP recognition element)-dependent RiPP (post-translationally modified peptides) biosynthesis. Moreover, the obtained results enhanced the knowledge about the salt mines microbiota biodiversity as a poorly explored environment so far.</p>","PeriodicalId":15834,"journal":{"name":"Journal of Genomics","volume":"11 ","pages":"20-25"},"PeriodicalIF":0.0,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10093198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9418856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}