{"title":"Enhancing the biodegradation of low-density polyethylene (LDPE) using novel bacterial consortia: Bacillus sp. AS3 and Sphingobacterium sp. AS8.","authors":"Pornya Khampratueng, Anil Kumar Anal","doi":"10.1016/j.jes.2025.04.007","DOIUrl":"https://doi.org/10.1016/j.jes.2025.04.007","url":null,"abstract":"<p><p>Pretreatment of Low-Density Polyethylene (LDPE) with physicochemical methods before biodegradation has been demonstrated as an effective strategy. The pretreatment of LDPE exhibited alterations in molecular structure, reducing hydrophobicity and decreasing tensile strength. Additionally, pretreating LDPE enhanced microbial biodegradability to improve biofilm formation and significantly reduced the physical weight of LDPE film. AS3-8 consortia exhibited a maximum weight loss of 8.0 % ± 0.5 % after 45 days of incubation. While Bacillus sp. AS3 and Sphingobacterium sp. AS8 demonstrated LDPE weight loss of 5.03 % ± 1.6 % and 1.6 % ± 0.5 %, respectively. The structure of LDPE was altered after incubation with the bacterial strains, resulting in a reduction in the intensity of functional groups, including C=O, C=C, N-H, and C-N. The carbonyl index (CI) of LDPE also decreased by 7.17 % after the consortia AS3-8 degradation. Consortia AS3-8 significantly impacted the physical properties of LDPE by reducing the water contact angle (WCA), decreasing to 64.21° ± 3.69°, and tensile strength (TS), decreasing to 17.97 ± 0.3 MPa. Moreover, the esterase activity was measured through 45 days of incubation. SDS-PAGE analysis of the AS3-8 consortia revealed bands at 35, 48, and 70 kDa molecular weights, similar to known enzymes like laccase and esterase. Furthermore, SEM observations showed rough, cracked surfaces on pretreated LDPE, with biofilms present after incubation with the bacterial strains. GC-MS analysis revealed that AS3-8 consortia produced depolymerized chemicals, including alkanes, aldehydes, and esters. The LDPE biodegradation pathway was elucidated. This study addresses critical knowledge gaps in improving plastic degradation efficiency.</p>","PeriodicalId":15788,"journal":{"name":"Journal of Environmental Sciences-china","volume":"159 ","pages":"263-270"},"PeriodicalIF":6.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A decision framework for rural domestic sewage treatment models and process: Evidence from Inner Mongolia Autonomous Region, China.","authors":"Ying Yan, Pengyu Li, Zixuan Wang, Yubo Tan, Tianlong Zheng, Jianguo Liu, Xiaoxia Yang, Junxin Liu","doi":"10.1016/j.jes.2025.05.016","DOIUrl":"https://doi.org/10.1016/j.jes.2025.05.016","url":null,"abstract":"<p><p>Rural domestic sewage treatment is critical for environmental protection. This study defines the spatial pattern of villages from the perspective of rural sewage treatment and develops an integrated decision-making system to propose a sewage treatment mode and scheme suitable for local conditions. By considering the village spatial layout and terrain factors, a decision tree model of residential density and terrain type was constructed with accuracies of 76.47 % and 96.00 %, respectively. Combined with binary classification probability unit regression, an appropriate sewage treatment mode for the village was determined with 87.00 % accuracy. The Analytic Hierarchy Process (AHP), combined with the Technique for Order Preference (TOPSIS) by Similarity to an Ideal Solution model, formed the basis for optimal treatment process selection under different emission standards. Verification was conducted in 542 villages across three counties of the Inner Mongolia Autonomous Region, focusing on the standard effluent effect (0.3773), low investment cost (0.3196), and high standard effluent effect (0.5115) to determine the best treatment process for the same emission standard under different needs. The annual environmental and carbon emission benefits of sewage treatment in these villages were estimated. This model matches village density, geographic feature, and social development level, and provides scientific support and a theoretical basis for rural sewage treatment decision-making.</p>","PeriodicalId":15788,"journal":{"name":"Journal of Environmental Sciences-china","volume":"159 ","pages":"302-311"},"PeriodicalIF":6.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145175648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Catalyst in-situ regeneration for polychlorinated biphenyl tail gas degradation and catalytic activity recovery mechanisms.","authors":"Zhichao Yun, Yuyang Bai, Fu Xia, Bingqing Wang, Qiyuan Liu, Yu Yang, Yonghai Jiang","doi":"10.1016/j.jes.2025.03.041","DOIUrl":"https://doi.org/10.1016/j.jes.2025.03.041","url":null,"abstract":"<p><p>To address the problems with catalytic degradation, such as poisoning and inactivation, a simple and efficient gas purging regeneration technique was developed for iron-based catalyst in-situ regeneration. Specifically, the effects of carrier gas types, regeneration temperatures, and granular activated carbon (GAC) addition on iron-based catalyst regeneration were investigated. The Fe<sub>3</sub>O<sub>4</sub>/γ-Al<sub>2</sub>O<sub>3</sub> regenerated at 550 °C with additional GAC and 15 % water vapor exhibited the optimal degradation efficiency towards polychlorinated biphenyls (PCBs), with an increase from 41.2 % to 93.5 %, compared with non-regenerated Fe<sub>3</sub>O<sub>4</sub>/γ-Al<sub>2</sub>O<sub>3</sub>. In addition, the 60-hour stability test revealed a well-recovered catalytic activity. During the Fe<sub>3</sub>O<sub>4</sub>/γ-Al<sub>2</sub>O<sub>3</sub> regeneration, the coke on the catalyst surface was oxidized and removed in the form of CO<sub>2</sub>, and meanwhile the oxidized Fe(III) was reduced into Fe(II) in the catalyst. This study provides a safe and efficient iron-based catalyst regeneration technology for PCB off-gas degradation and reveals the catalytic activity recovery mechanism during catalyst regeneration.</p>","PeriodicalId":15788,"journal":{"name":"Journal of Environmental Sciences-china","volume":"159 ","pages":"271-279"},"PeriodicalIF":6.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145175772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ferroptosis involved in inhaled polystyrene microplastics leaded myocardial fibrosis through HIF-ROS-SLC7A11/GPX4 Pathway.","authors":"Danyang Huang, Huiwen Kang, Ziyan Liu, Wei Zhang, Jingyu Wang, Ziyan Wang, Guangyu Jiang, Ai Gao","doi":"10.1016/j.jes.2025.04.059","DOIUrl":"https://doi.org/10.1016/j.jes.2025.04.059","url":null,"abstract":"<p><p>The issue of microplastic (MPs) pollution has received increased attention in recent years. Studies have indicated that inhalation of microplastics may result in the cardiovascular harm. However, the specific mechanism remains to be elucidated. In this study, 5 µm polystyrene microplastics (PS-MPs) were employed to construct in vivo and in vitro exposure models to investigate the potential mechanisms of microplastic-induced cardiac fibrosis. In vivo model of respiratory exposure to MPs, echocardiography observed a decrease in systolic-diastolic function of the mouse heart, and myocardial tissue showed significant mitochondrial morphological abnormalities and myocardial fibrosis. In vitro models also revealed upregulation of fibrosis indicators in human cardiomyocytes AC16 cells. Transcriptome and RT-qPCR assay exposed that ferroptosis-related pathways were significantly gathered in the MPs group, with decreased expression of ferroptosis related genes SLC7A11 and GPX4. Liproxstatin-1 (Lip-1), a ferroptosis inhibitor, significantly ameliorated MPs-induced cardiomyocyte fibrosis and ferroptosis. We further demonstrated that inhibition of hypoxia-inducible factor α (HIF-α) and oxidative stress ameliorated PS-MPs-induced cardiomyocyte ferroptosis, and thus upregulation of the HIF pathway and oxidative stress may be the upstream mechanism of MPs-induced ferroptosis in myocardial fibrosis. Above all, our study demonstrated that MPs exposure resulted in cardiac fibrosis via the HIF-ROS-SLC7A11/GPX4 signaling pathway.</p>","PeriodicalId":15788,"journal":{"name":"Journal of Environmental Sciences-china","volume":"159 ","pages":"391-402"},"PeriodicalIF":6.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145176147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xinyue Liu , Tianshan Zha , Charles P.-A. Bourque , Peng Liu , Hongxian Zhao , Tingshan Li , Xinhao Li , Yun Tian , Xin Jia
{"title":"Urban expansion drives both loss and compensation in city vegetation productivity","authors":"Xinyue Liu , Tianshan Zha , Charles P.-A. Bourque , Peng Liu , Hongxian Zhao , Tingshan Li , Xinhao Li , Yun Tian , Xin Jia","doi":"10.1016/j.jes.2025.08.014","DOIUrl":"10.1016/j.jes.2025.08.014","url":null,"abstract":"<div><div>Urbanization alters vegetation productivity by both direct (ω<sub>d</sub>) and indirect (ω<sub>i</sub>) effects. The direct effect is from the change of vegetated area indicated by impervious surface intensity (ISI), while indirect effects arise from changes in urban environmental factors, such as near-surface air temperatures, precipitation, urban heat island (UHI) intensity, and population density (POP). The respective contributions of ω<sub>d</sub> and ω<sub>i</sub> to vegetation net primary productivity (NPP) under various phases of urbanization are not well quantified. Using multisource remote-sensing data from 1990 to 2020, we analyzed the spatiotemporal variation in urban expansion and the effect that ω<sub>d</sub> and ω<sub>i</sub> had on NPP in the megalopolis of Beijing, China, over 5-year intervals. During this period, Beijing underwent significant planar expansion rates of about 58.9 km<sup>2</sup>/yr. Annual mean loss of NPP by ω<sub>d</sub> was estimated to be about 77.1 g C/(m<sup>2</sup>·yr) during the 1990-2020 period, while annual mean improvement to NPP by ω<sub>i</sub> amounted to an increase of 28.9 g C/(m<sup>2</sup>·yr). The NPP losses were partially offset by NPP improvements in the order of 18.6 %-69.3 %. The impact of forcing variables on NPP varied spatially. Air temperature, precipitation, UHI, POP, and ISI explained about 13.8 %, 23.2 %, 23.7 %, 14.7 %, and 24.6 % of the spatial variation in NPP. The impact of air temperature on NPP was related to available moisture, negatively affecting NPP in regions with water deficits. Our findings demonstrate the dual impact of urbanization on vegetation and underscore the necessity for spatially adaptive ecological management strategies in regions experiencing rapid urban growth.</div></div>","PeriodicalId":15788,"journal":{"name":"Journal of Environmental Sciences-china","volume":"160 ","pages":"Pages 591-599"},"PeriodicalIF":6.3,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Li Li , Hai Huang , Pei Hua , Tao Chen , Jin Zhang , Peng Deng , Zongxi Zhao , Bo Yan
{"title":"Machine learning combined with the PMF model reveals the sources and driving factors of PAHs and Cl-PAHs in urban runoff","authors":"Li Li , Hai Huang , Pei Hua , Tao Chen , Jin Zhang , Peng Deng , Zongxi Zhao , Bo Yan","doi":"10.1016/j.jes.2025.07.011","DOIUrl":"10.1016/j.jes.2025.07.011","url":null,"abstract":"<div><div>Urban rainwater runoff is an important source of nonpoint source pollution due to its transport of diverse contaminants, including polycyclic aromatic hydrocarbons (PAHs) and chlorinated derivatives. Importantly, these chlorinated polycyclic aromatic hydrocarbons (Cl-PAHs) exhibit elevated toxicological potential compared to their non-halogenated parent compounds. In this study, we proposed an approach that combined multivariate receptor model with integration of SHapley Additive exPlanations and Random Forest model. This method identifies the possible sources and reveals the impact of source apportionment results and environmental driving factors (such as geographical and meteorological data) on pollutant concentrations. Sixteen PAHs and nine Cl-PAHs were detected in 79 runoff samples from all three sites. The ∑<sub>16</sub>PAHs average concentration (2923.93 to 6071.83 ng/L) was significantly higher than the ∑<sub>9</sub>Cl-PAHs (384.34 to 1314.73 ng/L). The source apportionment was conducted by positive matrix factorization (PMF), and six potential pollution sources for PAHs and three for Cl-PAHs were quantified. PAHs primarily originate from the combustion of fossil fuels such as traffic, industrial emissions and coal tar, while Cl-PAHs are mainly derived from atmospheric deposition and industrial emissions. Meanwhile, the self‑organizing map classified PAHs and Cl-PAHs into 2 and 3 groups, respectively. The k-means algorithm yielded 4 clusters for runoff samples. Among machine learning models, Random Forest (RF) demonstrated optimal predictive performance and integrated with SHapley Additive exPlanations (RF-SHAP) revealed the effects of driving factors on the predicted concentration of PAHs and Cl-PAHs in urban runoff samples.</div></div>","PeriodicalId":15788,"journal":{"name":"Journal of Environmental Sciences-china","volume":"160 ","pages":"Pages 174-184"},"PeriodicalIF":6.3,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuxin Huang , Senchao Lai , Baoling Liang , Jinpu Zhang , Chenglei Pei , Dachi Hong , Xiaoluan Lin , Zhaokang Ruan , Leitao Sun , Yingyi Zhang
{"title":"Oxidative potential of PM2.5 in Guangzhou, Southern China: Source apportionment and association with airborne bacteria","authors":"Yuxin Huang , Senchao Lai , Baoling Liang , Jinpu Zhang , Chenglei Pei , Dachi Hong , Xiaoluan Lin , Zhaokang Ruan , Leitao Sun , Yingyi Zhang","doi":"10.1016/j.jes.2025.06.050","DOIUrl":"10.1016/j.jes.2025.06.050","url":null,"abstract":"<div><div>Oxidative potential (OP) can be used as an indicator of the health risks of particulate matter in the air. To study the variation and sources of OP, we conducted an observation of PM<sub>2.5</sub> in a megacity in southern China in winter and spring of 2021. The results show that the average concentration of PM<sub>2.5</sub> decreased by 47 % from winter to spring, while volume-normalized and mass-normalized OP (i.e., OP<sub>v</sub> and OP<sub>m</sub>) increased by 6 % and 69 %, respectively. It suggests that the decline of PM<sub>2.5</sub> may not necessarily decrease the health risks and the intrinsic toxicity of PM<sub>2.5</sub>. Variations of OP<sub>v</sub> and OP<sub>m</sub> among different periods were related to the different source contributions and environmental conditions. The positive matrix factorization model was used to identify the major sources of OP<sub>v</sub>. OP<sub>v</sub> was mainly contributed by biomass burning/industrial emissions (29 %), soil/road dust (20 %), secondary sulfate (14 %), and coal combustion (13 %) in winter. Different major sources were resolved to be secondary sulfate (36 %), biological sources (21 %), and marine vessels (20 %) in spring, presenting the substantial contribution of biological sources. The analysis shows strong associations between OP<sub>v</sub> and both live and dead bacteria, further confirming the important contribution of bioaerosols to the enhancement of OP. This study highlights the importance of understanding OP in ambient PM<sub>2.5</sub> in terms of public health impact and provides a new insight into the biological contribution to OP.</div></div>","PeriodicalId":15788,"journal":{"name":"Journal of Environmental Sciences-china","volume":"159 ","pages":"Pages 64-72"},"PeriodicalIF":5.9,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144713186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xuanwen Xu , Shuying Wang , Ying Luo , Pengyu Dong , Pengyu Wu , Zheng Wang , Ruiyu Jiang
{"title":"Coupling of the galvanic cell and Z-scheme effects of zinc/gallium dual co-catalysts to enhance the photocatalytic conversion of carbon dioxide using water as an electron donor","authors":"Xuanwen Xu , Shuying Wang , Ying Luo , Pengyu Dong , Pengyu Wu , Zheng Wang , Ruiyu Jiang","doi":"10.1016/j.jes.2025.05.062","DOIUrl":"10.1016/j.jes.2025.05.062","url":null,"abstract":"<div><div>Zinc/gallium (Zn/Ga) dual co-catalysts consisting of Zn<sup>0</sup> and amorphous ZnGa<sub>2</sub>O<sub>4</sub> species were found to selectively produce carbon monoxide (CO) during the photocatalytic conversion of carbon dioxide (CO<sub>2</sub>) using water (H<sub>2</sub>O) as an electron donor over photocatalysts such as NaTaO<sub>3</sub>, Ga<sub>2</sub>O<sub>3</sub>, and ZnGa<sub>2</sub>O<sub>4</sub>, and in the electrochemical reduction of CO<sub>2</sub> over Zn<sup>0</sup> electrodes. It is considered that there are two effects associated with the Zn/Ga dual co-catalysts: (1) a galvanic cell effect between Zn<sup>0</sup> and amorphous ZnGa<sub>2</sub>O<sub>4</sub>, and (2) a Z-scheme effect in NaTaO<sub>3</sub>/Zn<sup>0</sup>/amorphous ZnGa<sub>2</sub>O<sub>4</sub>. The coupling of these two effects favored the active and selective evolution of CO during the photocatalytic conversion of CO<sub>2</sub> by H<sub>2</sub>O.</div></div>","PeriodicalId":15788,"journal":{"name":"Journal of Environmental Sciences-china","volume":"160 ","pages":"Pages 610-620"},"PeriodicalIF":6.3,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. M. Khorshed Alam , Peiyue Li , Dan Wang , Misbah Fida , Vetrimurugan Elumalai
{"title":"Sources and transformations of shallow groundwater nitrate in intensively irrigated agricultural lands of the Yinchuan Plain, Northwest China","authors":"S. M. Khorshed Alam , Peiyue Li , Dan Wang , Misbah Fida , Vetrimurugan Elumalai","doi":"10.1016/j.jes.2025.06.022","DOIUrl":"10.1016/j.jes.2025.06.022","url":null,"abstract":"<div><div>Using unscientific agricultural methods can harm human health by increasing harmful nitrate (NO<sub>3</sub><sup>−</sup>) levels in groundwater, as observed in the Yinchuan Plain. This research utilized hydrochemical data, dual isotopic data, the MixSIAR model, and the uncertainty index (UI<sub>90</sub>) to detect the potential sources of groundwater NO<sub>3</sub><sup>−</sup>, track NO<sub>3</sub><sup>−</sup> conversion processes, and calculate the apportionment of each groundwater NO<sub>3</sub><sup>−</sup> source in the agricultural lands of the Yinchuan Plain. The results show that soil organic nitrogen accounted for 49.4 %, and N-fertilizers contributed 30.4 %, making them the two main contributors to NO<sub>3</sub><sup>-</sup> contamination in groundwater. Long-term N-fertilization enhances soil organic nitrogen accumulation, resulting in NO<sub>3</sub><sup>−</sup> leaching into groundwater during irrigation. The highest uncertainty regarding soil organic nitrogen and N-fertilizers may stem from changes in groundwater flow patterns, unbalanced N-fertilization, irrigation, and precipitation. Denitrification is the dominant process, resulting in lower NO<sub>3</sub><sup>−</sup> concentrations in groundwater in most areas. As a result, most groundwater in the Yinchuan Plain is generally safe for human consumption, except the specific areas in Qingtongxia City and Wuzhong City. Flood irrigation can increase the leaching of NO<sub>3</sub><sup>−</sup> into groundwater, and the repeated recharge of groundwater contaminated with high NO<sub>3</sub><sup>−</sup> levels could also be a potential source of NO<sub>3</sub><sup>−</sup> contamination in agricultural areas. This research provides scientific guidance for sustainable groundwater management in the Yinchuan Plain, mitigating the risk of groundwater NO<sub>3</sub><sup>−</sup> pollution.</div></div>","PeriodicalId":15788,"journal":{"name":"Journal of Environmental Sciences-china","volume":"160 ","pages":"Pages 670-681"},"PeriodicalIF":6.3,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of a whole-cell biosensor for the detection of low concentrations of tetracycline","authors":"Wenxiao Liu, Guohao Chen, Yue Li, Hongpo Dong","doi":"10.1016/j.jes.2025.06.018","DOIUrl":"10.1016/j.jes.2025.06.018","url":null,"abstract":"<div><div>By virtue of genetic engineering technology, we developed a highly sensitive biosensor for tetracycline detection based on the interaction between the tetracycline regulator TetR and tetracycline. In the absence of tetracycline, TetR binds to the <em>tetO</em> sequence, inhibiting the expression of the <em>sfGFP</em> reporter gene. When tetracycline is present, it induces TetR to release from <em>tetO</em>, allowing <em>sfGFP</em> expression. The biosensor was optimized through the selection of transcription factors and reporter genes, and the optimization of spacer lengths. Chassis cells were grown to mid-log phase in a tetracycline-supplemented medium for subsequent fluorescence intensity measurement. The biosensor exhibited a strong linear correlation between fluorescence intensity and tetracycline concentration (<em>I</em> = 37,620.7 × [C(Tc)] + 4048.5, <em>R</em>² = 0.998), demonstrating high sensitivity with a detection limit of 0.0097 mg/L. The response time of the biosensor ranged from 2 to 4 h within the working concentration range, making it suitable for real-time detection. It shows potential for application in actual water sample analysis and as an early warning technology for water pollution risks.</div></div>","PeriodicalId":15788,"journal":{"name":"Journal of Environmental Sciences-china","volume":"160 ","pages":"Pages 640-646"},"PeriodicalIF":6.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}