Journal of Endocrinology最新文献_第8页

Pro-resolving role of glucagon in lipopolysaccharide-induced mice lung neutrophilia. 胰高血糖素在脂多糖诱导小鼠肺中性粒细胞增多症中的促溶解作用。

IF 4 3区医学

Journal of Endocrinology Pub Date : 2023-09-01 DOI: 10.1530/JOE-22-0196

Daniella Bianchi Reis Insuela, Maximiliano Ruben Ferrero, Amanda da Silva Chaves, Diego de Sá Coutinho, Nathalia Dos Santos Magalhães, Ana Carolina Santos de Arantes, Adriana Ribeiro Silva, Patrícia Machado Rodrigues E Silva, Marco Aurélio Martins, Vinicius Frias Carvalho

{"title":"Pro-resolving role of glucagon in lipopolysaccharide-induced mice lung neutrophilia.","authors":"Daniella Bianchi Reis Insuela, Maximiliano Ruben Ferrero, Amanda da Silva Chaves, Diego de Sá Coutinho, Nathalia Dos Santos Magalhães, Ana Carolina Santos de Arantes, Adriana Ribeiro Silva, Patrícia Machado Rodrigues E Silva, Marco Aurélio Martins, Vinicius Frias Carvalho","doi":"10.1530/JOE-22-0196","DOIUrl":"https://doi.org/10.1530/JOE-22-0196","url":null,"abstract":"Prior research demonstrated that glucagon has protective roles against inflammation, but its effect on the resolution of inflammation remains elusive. Using in vitro and in vivo approaches, this study aimed to investigate the pro-resolving potential of glucagon on pulmonary neutrophilic inflammation caused by lipopolysaccharide. Lipopolysaccharide induced an increase in the proportions of neutrophils positives to glucagon receptor (GcgR) in vitro. In addition, lipopolysaccharide induced an increase in the neutrophil accumulation and expression of GcgR by the inflammatory cells in the lungs, however, without altering glucagon levels. Intranasal treatment with glucagon, at the peak of neutrophilic inflammation, reduced the neutrophil number in the bronchoalveolar lavage (BAL), and lung tissue within 24 h. The reduction of neutrophilic inflammation provoked by glucagon was accompanied by neutrophilia in the blood, an increase in the apoptosis rate of neutrophils in the BAL, enhance in the pro-apoptotic Bax protein expression, and decrease in the anti-apoptotic Bcl-2 protein levels in the lung. Glucagon also induced a rise in the cleavage of caspase-3 in the lungs; however, it was not significant. Glucagon inhibited the levels of IL-1β and TNF-α while increasing the content of pro-resolving mediators transforming growth factor (TGF-β1) and PGE2 in the BAL and lung. Finally, glucagon inhibited lipopolysaccharide-induced airway hyper-reactivity, as evidenced by the reduction in lung elastance values in response to methacholine. In conclusion, glucagon-induced resolution of neutrophilic inflammation by promoting cessation of neutrophil migration and a rise of neutrophil apoptosis and the levels of pro-resolving mediators TGF-β1 and PGE2.","PeriodicalId":15740,"journal":{"name":"Journal of Endocrinology","volume":"259 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10285061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recycling glucocorticoids: therapeutic implications of the 11β-HSD1 enzyme system. 循环糖皮质激素:11β-HSD1酶系统的治疗意义。

IF 4 3区医学

Journal of Endocrinology Pub Date : 2023-09-01 DOI: 10.1530/JOE-22-0289

Kevin H Tsai, Mark S Cooper

{"title":"Recycling glucocorticoids: therapeutic implications of the 11β-HSD1 enzyme system.","authors":"Kevin H Tsai, Mark S Cooper","doi":"10.1530/JOE-22-0289","DOIUrl":"https://doi.org/10.1530/JOE-22-0289","url":null,"abstract":"Endogenous glucocorticoids and commonly used oral glucocorticoids have the property of existing in an inactive and active form in vivo. The inactive form can be converted back to the active form, or 'recycled' in cells and tissues that express the 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) enzyme. This recycling provides an important contribution to the action of glucocorticoids. This review examines the literature relating to the importance of 11β-HSD1 activity during glucocorticoid treatment, with an emphasis on studies examining bone and joint disease and the ability of glucocorticoids to suppress inflammatory damage in models of arthritis. Animal models with global or selective deletion of 11β-HSD1 have determined the extent to which this recycling is important in normal physiology and during treatment with oral glucocorticoids. These studies demonstrate that 11β-HSD1-mediated recycling of inactive glucocorticoids has a substantial action and indeed is responsible for the majority of the effects of orally administered glucocorticoids on a range of tissues. Importantly, the anti-inflammatory actions of glucocorticoids appear largely through this mechanism such that mice that lack 11β-HSD1 are resistant to the anti-inflammatory actions of glucocorticoids. The recognition that to a large extent the circulating inactive counterpart of these glucocorticoids is more important to anti-inflammatory effects than the active glucocorticoid presents novel opportunities to more selectively target glucocorticoids to tissues or to reduce the likely side effects.","PeriodicalId":15740,"journal":{"name":"Journal of Endocrinology","volume":"258 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9914592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postmenopausal osteoporosis coexisting with sarcopenia: the role and mechanisms of estrogen. 绝经后骨质疏松伴骨骼肌减少症:雌激素的作用及机制。

IF 4 3区医学

Journal of Endocrinology Pub Date : 2023-09-01 DOI: 10.1530/JOE-23-0116

Lingyun Lu, Li Tian

引用次数: 0

Islet cells in human type 1 diabetes: from recent advances to novel therapies - a symposium-based roadmap for future research. 人类1型糖尿病中的胰岛细胞：从最新进展到新疗法——基于研讨会的未来研究路线图。

IF 4 3区医学

Journal of Endocrinology Pub Date : 2023-08-31 Print Date: 2023-09-01 DOI: 10.1530/JOE-23-0082

James Cantley, Decio Laks Eizirik, Esther Latres, Colin M Dayan

引用次数: 0

Mineralocorticoid receptor signalling in primary aldosteronism. 原发性醛固酮增多症中的盐皮质激素受体信号传导。

IF 4 3区医学

Journal of Endocrinology Pub Date : 2023-08-28 Print Date: 2023-09-01 DOI: 10.1530/JOE-22-0249

Jun Yang, Morag J Young, Timothy Cole, Peter J Fuller

引用次数: 0

RISING STARS: The heat is on: how does heat exposure cause pregnancy complications? 新星：高温：高温是如何导致妊娠并发症的？

IF 4 3区医学

Journal of Endocrinology Pub Date : 2023-08-25 Print Date: 2023-09-01 DOI: 10.1530/JOE-23-0030

Caitlin S Wyrwoll

{"title":"RISING STARS: The heat is on: how does heat exposure cause pregnancy complications?","authors":"Caitlin S Wyrwoll","doi":"10.1530/JOE-23-0030","DOIUrl":"10.1530/JOE-23-0030","url":null,"abstract":"The incidence and severity of heatwaves are increasing globally with concomitant health complications. Pregnancy is a critical time in the life course at risk of adverse health outcomes due to heat exposure. Dynamic physiological adaptations, which include altered thermoregulatory pathways, occur in pregnancy. If heat dissipation is ineffective, maternal and neonate health outcomes can be compromised. Indeed, epidemiological studies and animal models reveal that exposure to heat in pregnancy likely elicits an array of health complications including miscarriage, congenital anomalies, low birth weight, stillbirth, and preterm birth. Despite these associations, the reasons for why these complications occur are unclear. An array of physiological and endocrine changes in response to heat exposure in pregnancy likely underpin the adverse health outcomes, but currently, conclusive evidence is sparse. Accompanying these fundamental gaps in knowledge is a poor understanding of what exact climatic conditions challenge pregnant physiology. Moreover, the overlay of thermoregulatory-associated behaviours such as physical activity needs to be taken into consideration when assessing the risks to human health and identifying critical populations at risk. While the health impacts from heat are largely preventable through strategic interventions, for the related clinical practice, public health, and policy approaches to be effective, the gaps in basic science understanding urgently need to be addressed.","PeriodicalId":15740,"journal":{"name":"Journal of Endocrinology","volume":"259 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10646462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exercise prevents obesity by reducing gut-derived inflammatory signals to brown adipocytes in mice. 运动通过减少小鼠棕色脂肪细胞的肠道炎症信号来预防肥胖。

IF 4 3区医学

Journal of Endocrinology Pub Date : 2023-08-22 Print Date: 2023-09-01 DOI: 10.1530/JOE-23-0123

Daiana Araujo Santana-Oliveira, Henrique Souza-Tavares, Aline Fernandes-da-Silva, Flavia Maria Silva-Veiga, Gustavo Casimiro-Lopes, Patricia Cristina Lisboa, Carlos Alberto Mandarim-de-Lacerda, Vanessa Souza-Mello

{"title":"Exercise prevents obesity by reducing gut-derived inflammatory signals to brown adipocytes in mice.","authors":"Daiana Araujo Santana-Oliveira, Henrique Souza-Tavares, Aline Fernandes-da-Silva, Flavia Maria Silva-Veiga, Gustavo Casimiro-Lopes, Patricia Cristina Lisboa, Carlos Alberto Mandarim-de-Lacerda, Vanessa Souza-Mello","doi":"10.1530/JOE-23-0123","DOIUrl":"10.1530/JOE-23-0123","url":null,"abstract":"Gut dysbiosis impairs nonshivering thermogenesis (NST) in obesity. The antiobesogenic effects of exercise training might involve the modulation of gut microbiota and its inflammatory signals to the brown adipose tissue (BAT). This study evaluated whether high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) prevent overweight through reduced gut-derived inflammatory signals to BAT in high-fat-fed mice. Sixty male C57BL/6 mice (3 months old) comprised six experimental groups: control (C) diet group, C diet + HIIT (C-HIIT) group, C diet + MICT (C-MICT) group, high-fat (HF) diet group, HF diet + HIIT (HF-HIIT) group, and HF diet + MICT (HF-MICT) group. The protocols lasted for 10 weeks. HIIT and MICT restored body mass, mitigated glucose intolerance, and prevented hyperinsulinemia in HF-trained groups. A chronic HF diet caused dysbiosis, but HIIT and MICT prevented gut dysbiosis and preserved tight junction (TJ) gene expression. HF-HIIT and HF-MICT groups exhibited a similar pattern of goblet cell distribution, agreeing with the decreased plasma lipopolysaccharide concentrations and interscapular BAT (iBAT) Lbp-Cd14-Tlr4 expression. The lowered Nlrp3 and Il1β in the HF-HITT and HF-MICT groups complied with iBAT thermogenic capacity maintenance. This study shows reliable evidence that HIIT and MICT prevented overweight by restoring the diversity of the gut microbiota phyla and TJ gene expression, thereby reducing inflammatory signals to brown adipocytes with preserved thermogenic capacity. Both exercise modalities prevented overweight, but HIIT rescued Zo-1 and Jam-a gene expression, exerting more potent anti-inflammatory effects than MICT (reduced LPS concentrations), providing a sustained increase in thermogenesis with 78% less distance traveled.","PeriodicalId":15740,"journal":{"name":"Journal of Endocrinology","volume":"259 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10420161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human brown adipose tissue function: insights from current in vivo techniques. 人类棕色脂肪组织的功能：来自当前体内技术的见解。

IF 4 3区医学

Journal of Endocrinology Pub Date : 2023-08-18 Print Date: 2023-09-01 DOI: 10.1530/JOE-23-0017

T'ng Choong Kwok, Roland H Stimson

{"title":"Human brown adipose tissue function: insights from current in vivo techniques.","authors":"T'ng Choong Kwok, Roland H Stimson","doi":"10.1530/JOE-23-0017","DOIUrl":"10.1530/JOE-23-0017","url":null,"abstract":"The identification of brown adipose tissue (BAT) as a thermogenic organ in human adults approximately 20 years ago raised the exciting possibility of activating this tissue as a new treatment for obesity and cardiometabolic disease. [18F]Fluoro-2-deoxyglucose (18F-FDG) combined positron emission tomography and computed tomography (PET/CT) scanning is the most commonly used imaging modality to detect and quantify human BAT activity in vivo. This technique exploits the substantial glucose uptake by BAT during thermogenesis as a marker for BAT metabolism. 18F-FDG PET has provided substantial insights into human BAT physiology, including its regulatory pathways and the effect of obesity and cardiometabolic disease on BAT function. The use of alternative PET tracers and the development of novel techniques such as magnetic resonance imaging, supraclavicular skin temperature measurements, contrast-enhanced ultrasound, near-infrared spectroscopy and microdialysis have all added complementary information to improve our understanding of human BAT. However, many questions surrounding BAT physiology remain unanswered, highlighting the need for further research and novel approaches to investigate this tissue. This review critically discusses current techniques to assess human BAT function in vivo, the insights gained from these modalities and their limitations. We also discuss other promising techniques in development that will help dissect the pathways regulating human thermogenesis and determine the therapeutic potential of BAT activation.","PeriodicalId":15740,"journal":{"name":"Journal of Endocrinology","volume":"259 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10422659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The GLP-1 agonist, exendin-4, stimulates LH secretion in female sheep. GLP-1激动剂exendin-4刺激雌性绵羊的LH分泌。

IF 4 3区医学

Journal of Endocrinology Pub Date : 2023-08-17 Print Date: 2023-09-01 DOI: 10.1530/JOE-23-0105

Elizabeth M Simpson, Iain J Clarke, Christopher J Scott, Cyril P Stephen, Alexandra Rao, Allan J Gunn

{"title":"The GLP-1 agonist, exendin-4, stimulates LH secretion in female sheep.","authors":"Elizabeth M Simpson, Iain J Clarke, Christopher J Scott, Cyril P Stephen, Alexandra Rao, Allan J Gunn","doi":"10.1530/JOE-23-0105","DOIUrl":"10.1530/JOE-23-0105","url":null,"abstract":"Our previous studies showed that microinjection into the median eminence of the sheep of glucagon-like peptide- 1 (GLP-1) or its receptor agonist exendin-4 stimulates luteinising hormone (LH) secretion, but it is unknown whether the same effect may be obtained by systemic administration of the same. The present study measured the response in terms of plasma LH concentrations to intravenous (iv) infusion of exendin-4. A preliminary study showed that infusion of 2 mg exendin-4 into ewes produced a greater LH response in the follicular phase of the oestrous cycle than the luteal phase. Accordingly, the main study monitored plasma LH levels in response to either 0.5 mg or 2 mg exendin-4 or vehicle (normal saline) delivered by jugular infusion for 1 h in the follicular phase of the oestrous cycle. Blood samples were collected at 10 min intervals before, during and after infusion. Both doses of exendin-4 increased mean plasma LH concentrations and increased LH peripheral pulse amplitude. There was no effect on inter-pulse interval or timing of the preovulatory LH surge. These doses of exendin-4 did not alter plasma insulin or glucose concentrations. Quantitative PCR of the gastrointestinal tract samples from a population of ewes confirmed the expression of the preproglucagon gene (GCG). Expression increased aborally and was greatest in the rectum. It is concluded that endogenous GLP-1, most likely derived from the hindgut, may act systemically to stimulate LH secretion. The present data suggest that this effect may be obtained with levels of agonist that are lower than those functioning as an incretin.","PeriodicalId":15740,"journal":{"name":"Journal of Endocrinology","volume":"259 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10071076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of ACE2 on the susceptibility and vulnerability to COVID-19. ACE2对新冠肺炎易感性和易感性的影响。

IF 4 3区医学

Journal of Endocrinology Pub Date : 2023-08-14 Print Date: 2023-09-01 DOI: 10.1530/JOE-22-0262

Kirsty G Pringle, Lisa K Philp

{"title":"Impact of ACE2 on the susceptibility and vulnerability to COVID-19.","authors":"Kirsty G Pringle, Lisa K Philp","doi":"10.1530/JOE-22-0262","DOIUrl":"10.1530/JOE-22-0262","url":null,"abstract":"Angiotensin-converting enzyme 2 (ACE2) is not only the viral receptor for the novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) but is also classically known as a key carboxypeptidase, which through multiple interacting partners plays vital physiological roles in the heart, kidney, lung, and gastrointestinal tract. An accumulating body of evidence has implicated the dysregulation of ACE2 abundance and activity in the pathophysiology of multiple disease states. ACE2 has recently regained attention due to its evolving role in driving the susceptibility and disease severity of coronavirus disease 2019 (COVID-19). This narrative review outlines the current knowledge of the structure and tissue distribution of ACE2, its role in mediating SARS-CoV-2 cellular entry, its interacting partners, and functions. It also highlights how SARS-CoV-2-mediated dysregulation of membrane-bound and circulating soluble ACE2 during infection plays an important role in the pathogenesis of COVID-19. We explore contemporary evidence for the dysregulation of ACE2 in populations that have emerged as most vulnerable to COVID-19 morbidity and mortality, including the elderly, men, and pregnant women, and draw attention to ACE2 dynamics and discrepancies across the mRNA, protein (membrane-bound and circulating), and activity levels. This review highlights the need for improved understanding of the basic biology of ACE2 in populations vulnerable to COVID-19 to best ensure their clinical management and the appropriate prescription of targeted therapeutics.","PeriodicalId":15740,"journal":{"name":"Journal of Endocrinology","volume":"258 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10082031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0