Journal of embryology and experimental morphology最新文献_第9页

Basal laminar thinning in branching morphogenesis of the chick lung as demonstrated by lectin probes. 凝集素探针证实了鸡肺分支形态发生中的基底层变薄。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

B C Gallagher

引用次数: 0

Interdigital tissue chondrogenesis induced by surgical removal of the ectoderm in the embryonic chick leg bud. 手术切除鸡胚腿芽外胚层诱导指间组织软骨形成。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

J M Hurle, Y Gañan

{"title":"Interdigital tissue chondrogenesis induced by surgical removal of the ectoderm in the embryonic chick leg bud.","authors":"J M Hurle, Y Gañan","doi":"","DOIUrl":"","url":null,"abstract":"In the present work, we have analysed the possible involvement of ectodermal tissue in the control of interdigital mesenchymal cell death. Two types of experiments were performed in the stages previous to the onset of interdigital cell death: removal of the AER of the interdigit; removal of the dorsal ectoderm of the interdigit. After the operation embryos were sacrificed at 10-12 h intervals and the leg buds were studied by whole-mount cartilage staining, vital staining with neutral red and scanning electron microscopy. Between stages 27 and 30, ridge removal caused a local inhibition of the growth of the interdigit. In a high percentage of the cases, ridge removal at these stages was followed 30-40 h later by the formation of ectopic nodules of cartilage in the interdigit. The incidence of ectopic cartilage formation was maximum at stage 29 (60%). In all cases, cell death took place on schedule although the intensity and extent of necrosis appeared diminished in relation to the intensity of inhibition of interdigital growth and to the presence of interdigital cartilages. Ridge removal at stage 31 did not cause inhibition of the growth of the interdigit and ectopic chondrogenesis was only detected in 3 out of 35 operated embryos. Dorsal ectoderm removal from the proximal zone of the interdigit at stage 29 caused the chondrogenesis of the proximal interdigital mesenchyme in 6 out of 18 operated embryos. The pattern of neutral red vital staining was consistent with these results revealing a partial inhibition of interdigital cell death in the proximal zone of the interdigit. It is proposed that under the present experimental conditions the mesenchymal cells are diverted from the death programme by a primary transformation into cartilage.","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"94 ","pages":"231-44"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14879663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A physiological measure of shifting connections in the Rana pipiens retinotectal system. 一个转移连接的生理测量在蛙的视壁系统。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

S E Fraser, R K Hunt

{"title":"A physiological measure of shifting connections in the Rana pipiens retinotectal system.","authors":"S E Fraser, R K Hunt","doi":"","DOIUrl":"","url":null,"abstract":"The retinotectal connections of developing Rana tadpoles and froglets have been studied using light-pipe techniques to directly assay the pattern of the projection from the retina to the tectum. The projection site of the retina surrounding the optic nerve head was determined at two different stages of development (late larval and metamorphic frog) on the same animal. Small electrolytic marker lesions were used to mark the tectal sites to which the optic nerve head projected at these two times. Comparison of the positions of the two lesions gives a direct measure of the shift in the projection during the interlesion time interval of one week. The results indicate a shift in the projection of 275 micron week-1 in late larval life. Previous work in Xenopus using the light-pipe techniques indicated a qualitatively similar shift during equivalent stages of development, but significantly smaller in magnitude. In the present study, topographic postsynaptic units could be recorded at all stages investigated, indicating functional synapses between the optic nerve fibres and the tectum. Thus, these studies offer evidence of a significant shift in the functional connection pattern of the amphibian retinotectal map during development, in agreement with the recent anatomical data from other laboratories on the Rana and goldfish visual system.","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"94 ","pages":"149-61"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14613936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Some histochemical and ultrastructural observations on the early foetal pig testis. 早期胎猪睾丸的组织化学和超微结构观察。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

C J van Vorstenbosch, C M van Rossum-Kok, B Colenbrander, C J Wensing

引用次数: 0

Replacement of posterior by anterior endoderm reduces sterility in embryos from inverted eggs of Xenopus laevis. 用前内胚层代替后胚层可降低非洲爪蟾倒立卵胚胎的不育性。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

J H Cleine

{"title":"Replacement of posterior by anterior endoderm reduces sterility in embryos from inverted eggs of Xenopus laevis.","authors":"J H Cleine","doi":"","DOIUrl":"","url":null,"abstract":"The genital ridges of Xenopus laevis tadpoles reared from eggs kept in an inverted position contain less than 40% of the number of primordial germ cells (PGCs) of controls (Cleine & Dixon, 1985). It has been suggested that this reduction is caused by the germ cells' ectopic position in the anterior endoderm of larvae from inverted eggs, from where they may be unable to migrate into the genital ridges (Cleine & Dixon, 1985). This hypothesis is tested here by interchanging anterior and posterior endodermal grafts between pairs of inverted embryos at the early tailbud stage. Replacement of anterior by posterior endoderm has no effect but replacement of posterior by anterior endoderm increases the number of PGCs in the genital ridges and significantly reduces the proportion of sterile embryos. In a control series, in which the same type of grafting was done with normal embryos, replacement of posterior by anterior endoderm reduced the number of germ cells to almost zero, but replacement of anterior by posterior endoderm nearly doubled it. These findings are explained in terms of the distribution of the germ cells in the endoderm at the time of grafting. The results firstly show that the position of the germ cells is crucial to successful migration and secondly they support the notion that germ plasm has a determinative role during early germ cell differentiation.","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"94 ","pages":"83-93"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14879563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corneal epithelial-specific cell surface antigen recognized by a monoclonal antibody. 单克隆抗体识别的角膜上皮特异性细胞表面抗原。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

M G Langer, C V Sundarraj, N Sundarraj

{"title":"Corneal epithelial-specific cell surface antigen recognized by a monoclonal antibody.","authors":"M G Langer, C V Sundarraj, N Sundarraj","doi":"","DOIUrl":"","url":null,"abstract":"Monoclonal antibodies, specific against cell surface differentiation antigens of human corneal epithelial cells, were developed using epithelial cells resected from human corneas as the immunogens. One of these antibodies reacted specifically with corneal epithelial cells and not with epithelial cells of other tissues when tested by an indirect immunoperoxidase technique. Nonidet P-40 extracts of different subcellular fractions of human corneal epithelial cells were tested for their reactivity against this antibody using an enzyme-linked immunosorbent assay. The results indicated that the antigen recognized by this antibody is associated with the plasma membrane. This was further verified by immuno-electron-microscopic analysis using ferritin-conjugated anti-mouse IgG antibody. This antigen was not detectable in the corneal epithelial cells in primary cultures nor in the epithelial cells from early stages of developing cornea (12 to 18 weeks in utero) but was present in the epithelial cells in the corneas of an 8-month-old infant. Therefore, this surface-associated antigen identified in the present study is a developmentally regulated marker of human corneal epithelium.","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"94 ","pages":"163-72"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14879658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein requirements for germinal vesicle breakdown in ovine oocytes. 绵羊卵母细胞生发囊泡破裂所需的蛋白质。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

R M Moor, I M Crosby

{"title":"Protein requirements for germinal vesicle breakdown in ovine oocytes.","authors":"R M Moor, I M Crosby","doi":"","DOIUrl":"","url":null,"abstract":"The regulation of the cell cycle during the transition from prophase to metaphase I was studied by analysing protein changes and introducing protein blocks during the transition phase. The results show that the progression to metaphase in ovine oocytes is totally dependent on new protein synthesis. By delaying the addition of the inhibitor, cycloheximide, for progressively longer periods after the resumption of meiosis it was established that the required synthesis occurs in the 1-2 h immediately preceding germinal vesicle breakdown (GVBD). The action of cycloheximide was fully reversible: removal of the drug resulted in GVBD between 3 and 4 h later. The synthesis and modification of proteins during these first few hours of maturation were studied by short-term radiolabelling of oocytes with [35S]methionine and [32P]phosphate followed by rapid assessment of their precise nuclear configuration. Changes in phosphorylation of two polypeptides were detected 4-5 h after the beginning of culture, but these changes were not dependent upon protein synthesis. The earliest change in synthesis was the appearance of a new polypeptide 6-8 h after explantation, immediately before GVBD. This polypeptide (Mr 47 X 10(3), pI 5.8) was not significantly phosphorylated and was relatively stable. Oocytes released from cycloheximide treatment began to synthesize this molecule 3-4 h later, again coinciding with GVBD. Synthesis of the polypeptide was suppressed by inhibition of transcription with alpha-amanitin.","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"94 ","pages":"207-20"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14879661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Positional signalling by Hensen's node when grafted to the chick limb bud. 嫁接到鸡肢芽时，亨氏结的位置信号。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

A Hornbruch, L Wolpert

引用次数: 0

The timing of compaction: control of a major developmental transition in mouse early embryogenesis. 压实的时间:小鼠早期胚胎发生中一个主要发育转变的控制。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

J B Levy, M H Johnson, H Goodall, B Maro

引用次数: 0

Sensory nerve routes in chick wing buds deprived of motor innervation. 失去运动神经支配的鸡翅芽感觉神经通路。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

G J Swanson, J Lewis

引用次数: 0