Journal of embryology and experimental morphology最新文献_第10页

Changes in the transferrin requirement of cultured chick embryo mesoderm cells during early differentiation. 培养鸡胚中胚层细胞早期分化过程中转铁蛋白需求量的变化。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

E J Sanders

{"title":"Changes in the transferrin requirement of cultured chick embryo mesoderm cells during early differentiation.","authors":"E J Sanders","doi":"","DOIUrl":"","url":null,"abstract":"Mesodermal tissue from the chick embryo at various stages of early differentiation was cultured in hydrated gels of type I collagen in the presence and absence of transferrin. Primary mesoderm explants from primitive-streak-stage embryos responded to the presence of avian transferrin by significantly improved outgrowth which appeared to be related to the ability of the cells to attach to, and migrate in, the collagen. No evidence was obtained which suggested that this observation was dependent on increased cell proliferation. This outgrowth enhancement was not duplicated by transferrin of human origin. The avian transferrin did not produce this effect on cells cultured on plastic substrata, suggesting that the species-specific effect involves modulation by the extracellular matrix. Mesoderm explants from somite stages of development showed no increase in outgrowth in the presence of either avian or human transferrin as judged by counting the number of outwandering cells. Ultrastructural immunocytochemistry indicated surface binding of transferrin by cells in the gels, and the presence of endogenous transferrin on the surfaces of mesoderm cells in situ and in their extracellular environment. It is suggested that by binding to cell surface receptors, transferrin may be able to influence the strength of cellular adhesion to collagen and hence the capacity for cell locomotion.","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"95 ","pages":"81-93"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14913232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Positional signalling by Hensen's node when grafted to the chick limb bud. 嫁接到鸡肢芽时，亨氏结的位置信号。

Journal of embryology and experimental morphology Pub Date : 1986-06-01 DOI: 10.1242/DEV.94.1.257

A. Hornbruch, L. Wolpert

引用次数: 112

The pituitary adrenocorticotropes originate from neural ridge tissue in Xenopus laevis. 垂体促肾上腺皮质激素来源于非洲爪蟾的神经脊组织。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

G W Eagleson, B G Jenks, A P Van Overbeeke

{"title":"The pituitary adrenocorticotropes originate from neural ridge tissue in Xenopus laevis.","authors":"G W Eagleson, B G Jenks, A P Van Overbeeke","doi":"","DOIUrl":"","url":null,"abstract":"A series of grafting experiments was conducted to determine pituitary origins prior to brain tube closure in Xenopus laevis. Extirpation experiments indicated that the ventral neural ridge (VNR) tissue of stage-18+ embryos was essential for pituitary development. Bolton-Hunter reagent was used to label stage-18+ VNR tissue with 125I, and this tissue was then returned to the donor and its subsequent ontogenesis followed. Labelled tissue was ultimately found in the ventral hypothalamus, the ventral retina, and the anterior pituitary. Using immunocytochemical techniques with antisera to adrenocorticotropin (ACTH), it was found that some of the VNR-derived cells were corticotropes. A region of the nucleus infundibularis which was radioactive labelled also gave ACTH-positive immunoreaction. This might indicate that some ACTH-containing neurones of the hypothalamus are VNR in origin. We suggest that stage-18+ VNR is the site of attachment of brain and anterior pituitary ectoderm. Part of this adherence point is eventually incorporated into the anterior pituitary and will form corticotropes. It is concluded that the ventral retina, the preoptic region of the hypothalamus, some hypothalamic ACTH-immunoreactive cells, and the most anterior portion of the adenohypophysis are all ventral neural ridge in origin.","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"95 ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14157952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chimaerism of primordial germ cells in the early postimplantation mouse embryo following microsurgical grafting of posterior primitive streak cells in vitro. 后原始条纹细胞显微外科移植后早期小鼠胚胎中原始生殖细胞的嵌合。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

A J Copp, H M Roberts, P E Polani

{"title":"Chimaerism of primordial germ cells in the early postimplantation mouse embryo following microsurgical grafting of posterior primitive streak cells in vitro.","authors":"A J Copp, H M Roberts, P E Polani","doi":"","DOIUrl":"","url":null,"abstract":"A microsurgical grafting technique has been used to introduce primordial germ cell (PGC) precursors into intact primitive-streak-stage mouse embryos in vitro. Operated embryos were cultured for 36-40 h and then analysed by a combined histochemical and autoradiographic method. PGC chimaerism occurred in embryos that received grafts of caudal primitive streak cells but not adjacent embryonic endoderm or anterolateral ectoderm/mesoderm cells. Graft-derived PGCs were found to be migrating through the gut endoderm alongside host-derived PGCs in approximately half of the chimaeric embryos whereas in the other 50% of cases PGCs remained at the site of grafting in association with graft-derived somatic cells. A similar pattern of somatic chimaerism was produced by primitive streak and anterolateral ectoderm/mesoderm grafts: the allantois was colonized predominantly, with, in addition, formation of amnion, surface ectoderm and caudal mesoderm in a few embryos. The majority of embryonic endoderm grafts failed to incorporate into host embryos and formed yolk-sac-like vesicles. The findings of this study indicate that PGCs originate from the embryonic ectoderm via the primitive streak during development of the mouse embryo, and anterolateral ectoderm and mesoderm cells are unable to form PGCs after heterotopic grafting to the posterior primitive streak site. The combined microsurgical and embryo culture methods provide an experimental system for the analysis of PGC development in intact mouse embryos.","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"95 ","pages":"95-115"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14760532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rat embryonic ectoderm as renal isograft. 大鼠胚胎外胚层作为肾同种移植物。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

A Svajger, B Levak-Svajger, N Skreb

引用次数: 0

The regeneration of double dorsal and double ventral limbs in the axolotl. 美西螈双背、双腹肢的再生。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

R Burton, N Holder, P Jesani

引用次数: 0

The cellular origin of fibronectin in the basement membrane zone of developing tooth. 发育中的牙齿基膜区纤维连接蛋白的细胞起源。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

K Hurmerinta, P Kuusela, I Thesleff

{"title":"The cellular origin of fibronectin in the basement membrane zone of developing tooth.","authors":"K Hurmerinta, P Kuusela, I Thesleff","doi":"","DOIUrl":"","url":null,"abstract":"The cellular source of fibronectin in the dental epitheliomesenchymal interface was studied in interspecies combinations of mouse and quail tissue. Species-specific fibronectin antibodies were produced by immunizing rabbits with purified mouse or chicken fibronectin and by absorbing both antisera with purified heterologous fibronectin and insoluble tissue extract. The absorbed antisera to mouse and chicken fibronectin showed fluorescent staining only in mouse and chicken tissue sections, respectively, but not vice versa. When the mouse mesenchymal dental papilla was combined and cultured either with the mouse enamel organ or with the quail pharyngeal epithelium, mesenchymal cell differentiation was initiated and typical alignment of mesenchymal cells along the basement membrane was seen. Examination with transmission electron microscope revealed a typical bilaminar basal lamina with adherent fibrillar matrix on its mesenchymal aspect. Immunofluorescent localization of fibronectin with the mouse-specific fibronectin antiserum showed a brilliant staining in the mesenchymal tissue and in the basement membrane zone. When the chicken-specific fibronectin antiserum was used, no staining was detected in either tissue recombinations. We have suggested earlier that fibronectin in the dental basement membrane plays an important role during the differentiation of mesenchymal cells into odontoblasts. The present study demonstrates that fibronectin in the basement membrane of the developing tooth is produced exclusively by the differentiating mesenchymal cells.","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"95 ","pages":"73-80"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14662631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal and spatial aspects of the gradual migration of primordial germ cells from the epiblast into the germinal crescent in the avian embryo. 原始生殖细胞在鸟类胚胎中从外胚层逐渐迁移到生发月牙的时间和空间方面。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

M Ginsburg, H Eyal-Giladi

引用次数: 0

Fate of brachial muscles of the chick embryo innervated by inappropriate nerves: structural, functional and histochemical analyses. 不适当神经支配的鸡胚胎臂肌的命运:结构、功能和组织化学分析。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

J Butler, P Cauwenbergs, E Cosmos

引用次数: 0

The application of aqueous two-phase partition to the study of chick limb mesenchymal diversification. 水两相分割法在鸡肢体间质分化研究中的应用。

Journal of embryology and experimental morphology Pub Date : 1986-06-01

C P Cottrill, P T Sharpe, L Wolpert

引用次数: 0