Journal of embryology and experimental morphology最新文献

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Genetic activity at the albino locus in Cattanach's insertion in the mouse. 卡塔纳赫在小鼠体内植入白化位点的遗传活性。
M S Deol, G M Truslove, A McLaren
{"title":"Genetic activity at the albino locus in Cattanach's insertion in the mouse.","authors":"M S Deol,&nbsp;G M Truslove,&nbsp;A McLaren","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cattanach's insertion (Is(In7;X)1Ct or XCt) includes the normal allele at the albino locus (c+), which is subject to inactivation of the X chromosome carrying it, so that XCtX; cc mice have albino and pigmented patches. The X-autosome translocation T(X;16)16H or XT16H leads to preferential inactivation of the other X chromosome in female cells, so that XCtXT16H; cc mice are almost entirely white. However, they grow darker with age, as if reversal of inactivation of the c+ allele were taking place in increasing numbers of melanocytes. To test whether this is dependent only on age or whether it is related to the number of times the animal has moulted, hair was repeatedly plucked from selected areas at the early telogen stage when the follicles are also removed, assuming that the melanocytes or melanoblasts in that region of the skin would be forced to undergo further divisions to colonize the new follicles. The plucked areas grew darker at the same rate as the rest of the coat, suggesting that the progressive reversal of inactivation is dependent only on age. As direct examination of melanocytes in the follicles is difficult, they were examined in the choroid and the retinal pigment epithelium (RPE) of the eye. The frequency of the pigmented cells was lower in the choroid than in the RPE. Since the melanocytes in these structures are different in origin as well as in physical characteristics, it appears that cell type influences either reversal of inactivation, or the frequency with which the influence of the X chromosome extends to the albino locus.</p>","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"96 ","pages":"295-302"},"PeriodicalIF":0.0,"publicationDate":"1986-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14160016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The clonal model of vertebral column development: a reinvestigation of vertebral shape using Fourier analysis. 脊柱发育的克隆模型:利用傅里叶分析对椎体形状的重新研究。
P O'Higgins, D R Johnson, T J McAndrew
{"title":"The clonal model of vertebral column development: a reinvestigation of vertebral shape using Fourier analysis.","authors":"P O'Higgins,&nbsp;D R Johnson,&nbsp;T J McAndrew","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Little work has been done on the skeletal system of allophenic mice (chimaeras). In this paper we re-examine the evidence presented for the clonal theory of vertebral development using quantitative methods which take account of variation. We conclude that the clonal theory is either an overly simplistic approximation of the real situation, or that the evidence so far given in its support is unsubstantiated.</p>","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"96 ","pages":"171-82"},"PeriodicalIF":0.0,"publicationDate":"1986-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14922542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The histogenetic potential of neural plate cells of early-somite-stage mouse embryos. 早体期小鼠胚胎神经板细胞的组织发生潜能。
W Y Chan, P P Tam
{"title":"The histogenetic potential of neural plate cells of early-somite-stage mouse embryos.","authors":"W Y Chan,&nbsp;P P Tam","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The mesencephalic neural plate of early-somite-stage mouse embryos differentiated underneath the renal capsule to form mostly neural tissues together with other tissues some of which were probably of neural crest cell origin. The capacity to form non-neural tissues such as skeletal tissues and melanocytes was lost at about the 5-somite stage. The lateral areas of the plate tended to form non-neural tissues more than the medial areas. The cephalic neural plate of presomite head-fold-stage embryos differentiated extensively to form both ectodermal and mesodermal tissues. However, upon completion of neurulation, the mesencephalic neuroepithelium of forelimb-bud-stage embryos gave rise to neural tissues only. Therefore there is a progressive restriction in the histogenetic capacity of the mesencephalic neural plate during neurulation and this could be attributed to the cellular commitment for neural differentiation and the loss of the neural crest cells.</p>","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"96 ","pages":"183-93"},"PeriodicalIF":0.0,"publicationDate":"1986-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14922543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Disruption of segmentation in a short germ insect embryo. I. The location of abnormalities induced by heat shock. 短胚芽昆虫胚胎的节段断裂。1、热休克引起的异常部位。
Journal of embryology and experimental morphology Pub Date : 1986-07-01 DOI: 10.1242/DEV.96.1.245
J. E. Mee, V. French
{"title":"Disruption of segmentation in a short germ insect embryo. I. The location of abnormalities induced by heat shock.","authors":"J. E. Mee, V. French","doi":"10.1242/DEV.96.1.245","DOIUrl":"https://doi.org/10.1242/DEV.96.1.245","url":null,"abstract":"The effect of heat shock (15 min at 48 degrees C) on segmentation has been investigated in the short germ embryo of the locust (Schistocerca gregaria). Prior to formation of the germ anlage and at the disc stage heat shock considerably reduced the survival of eggs but appeared to have little effect upon segmentation. At later stages heat shock had no effect on survival but resulted in disruptions of the segmental pattern. The location of abnormal segments depended upon the stage at heat shock and the number affected depended on its severity. A constant number of normal segments developed between the last segment visible at the time of heat shock and the first abnormal segment. These results are similar to the disruptions observed in amphibian somites following heat shock. However, different parts of the segment pattern varied in their response; the head segments were very rarely affected, and disrupted regions rarely started in the middle abdomen (segments A5 and A6). The results are discussed in relation to two models (the clock and wavefront and progress zone models) that have been proposed as an explanation for the specification of the somite pattern in amphibians.","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"18 1","pages":"245-66"},"PeriodicalIF":0.0,"publicationDate":"1986-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73467122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Induction of hair follicles in mouse skin by rat vibrissa dermal papillae. 大鼠触须真皮乳头诱导小鼠皮肤毛囊的研究。
P Pisansarakit, G P Moore
{"title":"Induction of hair follicles in mouse skin by rat vibrissa dermal papillae.","authors":"P Pisansarakit,&nbsp;G P Moore","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Rat vibrissa dermal papillae were transplanted between the epidermis and dermis of isolated embryonic mouse skin and then grafted onto nude mice. The papillae induced the formation of hair follicles which were larger than those of the host skin but smaller than vibrissa follicles. The potential of isolated dermal papillae to induce follicles with characteristics of those from which the papillae originated is discussed. One of the major factors affecting the sizes of induced follicles may have been related to the splitting of the papilla mass and dispersal of the cells by invading cords of epidermal cells from the host skin during induction.</p>","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"94 ","pages":"113-9"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14759314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Size regulation in the mouse embryo. I. The development of quadruple aggregates. 小鼠胚胎的大小调节。一、四重聚集体的发展。
G F Rands
{"title":"Size regulation in the mouse embryo. I. The development of quadruple aggregates.","authors":"G F Rands","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The development of mouse embryos formed by the aggregation of four 8-cell-stage eggs was analysed in comparison with control single embryos. The analysis revealed that: Quadruple aggregates undergo size regulation over several days, starting before implantation and being completed by 6.5 days post coitum. The attainment of recognizable postimplantation morphological stages is independent of size. Regulation is not brought about by disproportionate alterations in the size of the internal cavities. Regulation in both inner cell mass (ICM) and trophectoderm derivatives is completed between 5.5 and 6.5 days post coitum. Despite the abnormal proportions of ICM and trophectoderm in quadruple blastocysts, the proportions of the tissues derived from them are already normal by 5.5 days. The possibility that down regulation in size of aggregate embryos occurs as a consequence of limited nutrient supply is discussed.</p>","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"94 ","pages":"139-48"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14879657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Branching morphogenesis in the avian lung: electron microscopic studies using cationic dyes. 禽肺分支形态发生:阳离子染料电镜研究。
B C Gallagher
{"title":"Branching morphogenesis in the avian lung: electron microscopic studies using cationic dyes.","authors":"B C Gallagher","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The developing chick lung was examined in the electron microscope for intimate cell contacts between epithelium and mesenchyme, discontinuities in the basal lamina and substructure of the basement membrane. Cell filopodia were seen which crossed the basal lamina from both the epithelial and the mesenchymal cells. Ruthenium red and tannic acid staining of the basal lamina of the chick lung showed it to be thin and sometimes discontinuous at the tips compared to the more substantial basal lamina in the interbud areas. The bilaminar distribution of particles seen with ruthenium red is similar to those seen in the cornea and lens. With tannic acid staining, filaments could be seen which crossed the lamina lucida and connected with the lamina densa. Spikes perpendicular to the basal lamina were sometimes seen with a periodicity of approximately 110 nm. Alcian blue staining revealed structure similar to that seen by ruthenium red staining in the salivary and mammary glands, although the interparticle spacing was closer. Collagen was located in areas of morphogenetic stability, as has been seen by other investigators in different tissues. Collagen was coated with granules (probably proteoglycan) at periodic intervals when stained with ruthenium red. The fibrils were oriented circumferentially around the mesobronchus and were assumed to continue into the bud, but the fibres curve laterally at the middle of a bud. This orientation is opposite to that seen by another investigator in the mouse lung. In general, the observations made in the avian lung are similar to those seen in branching mammalian tissue. It is likely, therefore, that the chick lung uses strategies in its morphogenesis that are similar to those that have been elucidated previously in developing mammalian organs.</p>","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"94 ","pages":"189-205"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14879660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of the cytosolic proteins of chick embryo gonads by two-dimensional gel electrophoresis. 鸡胚性腺细胞质蛋白的双向凝胶电泳分析。
J Samsel, B Lorber, A Petit, J P Weniger
{"title":"Analysis of the cytosolic proteins of chick embryo gonads by two-dimensional gel electrophoresis.","authors":"J Samsel,&nbsp;B Lorber,&nbsp;A Petit,&nbsp;J P Weniger","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Gonads and mesonephros dissected from normal chick embryos, as well as a blood sample, were labelled in vitro with [35S]methionine and [14C]leucine. The patterns of cytosolic protein synthesis of the different tissues were analysed using two-dimensional (2-D) polyacrylamide gel electrophoresis. Three developmental stages, i.e. 8, 12 and 17 days of incubation, were investigated. Five sex-specific proteins were detected in the male and two in the female. In the testis, only one protein is already present at the 8-day stage. In the ovary, one protein exists since the 8-day stage, but it is also synthesized in the mesonephros. The second ovary-specific protein appeared only at the 12-day stage.</p>","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"94 ","pages":"221-30"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14879662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electric fields, contact guidance and the direction of nerve growth. 电场,接触引导和神经生长方向。
C D McCaig
{"title":"Electric fields, contact guidance and the direction of nerve growth.","authors":"C D McCaig","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Nerve orientation in response to electrical guidance cues in one direction and contact guidance cues in an orthogonal direction has been studied. Where neurites had a free choice between following contact guidance cues or electrical cues, the direction of nerve growth was determined predominantly by the vector of the applied electric field.</p>","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"94 ","pages":"245-55"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14879664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
What do dissociated embryonic cells of the starfish, Asterina pectinifera, do to reconstruct bipinnaria larvae? 海星(Asterina pectinifera)分离出的胚胎细胞如何重建双翅虫幼虫?
H Yamanaka, Y Tanaka-Ohmura, M Dan-Sohkawa
{"title":"What do dissociated embryonic cells of the starfish, Asterina pectinifera, do to reconstruct bipinnaria larvae?","authors":"H Yamanaka,&nbsp;Y Tanaka-Ohmura,&nbsp;M Dan-Sohkawa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The cellular events that take place during reconstruction of larval forms from dissociated embryonic cells of the starfish are investigated by thick and thin sections. Dissociated cells reaggregate, form an external epithelium (ectoderm), internal epithelial vesicles (endoderm), the blastocoel and the mesenchyme. The internal vesicles continue to fuse until there is only one large one suspended in the centre of the blastocoel. Eventually, the ectoderm invaginates at one or more sites and fuses with the endoderm to form blastopore(s). Special emphasis is placed on the differences in cell behaviour during endoderm-to-endoderm and endoderm-to-ectoderm fusion.</p>","PeriodicalId":15708,"journal":{"name":"Journal of embryology and experimental morphology","volume":"94 ","pages":"61-71"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14879562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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