Journal of Complementary and Integrative Medicine最新文献

{"title":"Antibacterial activity of Macrosciadium alatum (M.Bieb.) plant extract","authors":"H. Mammadova","doi":"10.1515/jcim-2023-0306","DOIUrl":"https://doi.org/10.1515/jcim-2023-0306","url":null,"abstract":"Abstract Objectives The flora of Azerbaijan is represented by one species of the Macrosciadium genus: Macrosciadium alatum, belonging to the Apiaceae family. It is commonly found in the Greater and Lesser Caucasus regions of Azerbaijan, as part of subalpine meadow plant communities. M. alatum is characterized by its robust, thick, tuberous roots, long-petioled and several times pinnately divided leaves, numerous (30–50) white umbels, and oval-shaped fruits. The primary objective of this research is to determine the antimicrobial potential of the aqueous extract obtained from M. alatum against both Gram-negative and Gram-positive bacteria. The plant preparations utilized in in vitro experiments were in the form of maceration, infusion, and hydrodistillation as aqueous extracts. M. alatum extract exhibited maximum (measuring 22.3 ± 1.4 mm) inhibition zones against bacteria (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, and Salmonella enteritidis) strains. Following exposure to the M. alatum plant extract, a significant reduction in bacterial cell cytoplasmic pH was observed (p≤0.04). Methods In order to investigate the antimicrobial effects of the plant extract, commonly accepted procedures were followed using well-known bacterial strains, including S. aureus, B. cereus, E. coli, S. enteritis and P. aeruginosa, which are principal causative agents of purulent-inflammatory processes. The 20 % aqueous extract was used. Results The conducted experiment to determine the impact of the plant extract on microorganisms revealed that the extract significantly affects the bacterial cell membrane. Specifically, there is a decrease in pH, and hyperpolarization of the cell membrane occurs. The efficacy of the preservative effect is highly dependent on the environmental pH. 1. The 20 % aqueous extract from exhibited antimicrobial activity and effectively preventing the development of foodborne pathogens and putrefactive microorganisms. 2. A 20 % aqueous extract of M. alatum exhibits antimicrobial activity, effectively inhibiting the growth of foodborne pathogens and spoilage microorganisms. 3. Extract led to an increase in H+ concentration within bacterial cell cytoplasm, surpassing the OH− concentration. 4. M. alatum species has a significant inhibitory effect on the growth of microorganisms such as S. aureus, E. coli, P. aeruginosa, and S. enteritidis. Conclusions The results suggest that the extract from M. alatum possesses antimicrobial properties, making it a potential candidate for use as a natural food preservative. The observed hyperpolarization of the cell membrane and pH reduction further support its potential as an effective antibacterial agent.","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141334915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of anti-nociceptive and anti-inflammatory activities of solvent fraction of the roots of <i>Echinops kebericho</i> Mesfin (Asteraceae) in mice model.","authors":"Tesfaye Yimer Tadesse, Samuel Berihun Dagnew, Tesfagegn Gobezie Yiblet, Getu Tesfaw Addis, Zemene Demelash Kiflie","doi":"10.1515/jcim-2024-0133","DOIUrl":"10.1515/jcim-2024-0133","url":null,"abstract":"<p><strong>Objective: </strong>The present study was aimed at investigating the antinociceptive and anti-inflammatory activities of the solvent fractions of the roots of <i>Echinops kebericho</i> Mesfin in rodent models of pain and inflammation.</p><p><strong>Methods: </strong>Successive maceration was used as a method of extraction using solvents of increasing polarity: methanol and water. Ethyl acetate, chloroform and distilled water were used as solvents of the fraction process. Swiss albino mice models were used in acetic acid induced writhing, hot plate, carrageenan induced paw edema and cotton pellet granuloma to assess the analgesic and anti-inflammatory activities. The test groups received different doses (100 mg/kg, 200 mg/kg and 400 mg/kg) of the three fractions (chloroform, ethyl acetate and aqueous). The positive control groups received ASA (150 mg/kg) for the writing test, morphine (10 mg/kg) for the hot plate method, diclofenac Na for carrageenan-induced paw edema, and dexamethasone (10 mg/kg) for granuloma, while the negative control group received distilled water.</p><p><strong>Results: </strong>EA fraction at all test doses employed (100 mg/kg, 200 mg/kg, and 400 mg/kg) showed statistically significant (p<0.05, p<0.01, p<0.001 respectively) analgesic and anti-inflammatory activities in a dose-dependent manner. The AQ fraction on the other hand produced statistically significant (p<0.05, p<0.012) analgesic and anti-inflammatory activities at the doses of 200 mg/kg and 400 mg/kg, while the CH fraction exhibited statistically significant (p<0.05) analgesic and anti-inflammatory activity at the dose of 400 mg/kg.</p><p><strong>Conclusions: </strong>In general, the data obtained from the present study elucidated that the solvent fractions of the study plant possessed significant analgesic and anti-inflammatory activities and were recommended for further investigations.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"314-324"},"PeriodicalIF":0.0,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141419406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A spectrometric analysis of variedly purified cinnabar in a siddha drug - <i>linga chendhooram</i>.","authors":"Shivani Gopalakrishnan, Baskaran Sivaraj, Hariharan Pattabiraman","doi":"10.1515/jcim-2024-0052","DOIUrl":"10.1515/jcim-2024-0052","url":null,"abstract":"<p><strong>Objectives: </strong>A siddha drug, <i>linga chendhooram</i> was prepared from cinnabar (<i>lingam</i>) and <i>Citrullus colocynthis</i> L. The transition of heavy metals concentration throughout the process were analysed to comprehend the significance of drug preparatory methods. In addition to that the main constituent cinnabar was purified from two different methods to comparatively analyse the significance of purification methods.</p><p><strong>Methods: </strong>Atomic absorption spectrometric studies were employed to obtain the concentration of class I heavy metals (lead, arsenic, cadmium and mercury) in all five samples - raw cinnabar (R), cinnabar purified (P1 and P2) from purification method 1 and 2, <i>linga chendhooram</i> (D1 and D2) prepared from P1 and P2.</p><p><strong>Results: </strong>Based on the permissible limits given by The Ayurvedic Pharmacopoeia of India, except cadmium the concentrations of all other three heavy metals were found to be above the limit in P1, P2, D1 and D2. According to the oral Permitted Daily Exposure values for elemental impurities given in ICH Q3D(R1) Guidelines, D1 was within the permissible limits for both single and double oral doses whereas D2 showed a slight increase in the concentration of lead in a single oral dose itself.</p><p><strong>Conclusions: </strong>The drug preparatory method showed a reduction of the heavy metals concentration and comparative analysis of the purification methods revealed a substantial reduction of heavy metals concentration in the end drug D1 when compared with D2 showing that the methods of purification and drug preparation in siddha system plays a vital role in reducing the concentrations of heavy metals accordingly.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"325-330"},"PeriodicalIF":0.0,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141161464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory, anti-oxidant and anti-ulcer activities of aqueous lyophilizate of <i>Markhamia lutea</i> (Bignoniaceae).","authors":"Noungoua Mbeugangkeng Chrétien, Fagni Njoya Zenab Linda, Nzeumo Nziid Brice Junior, Feigni Youyi Marcelle Olga, Matah Marthe Vanessa Mba, Djuichou Nguemnang Stephanie Flore, Madjo Kouam Yacine Karelle, Ngoufack Azanze Elvira, Mbiantcha Marius, Ateufack Gilbert","doi":"10.1515/jcim-2024-0059","DOIUrl":"10.1515/jcim-2024-0059","url":null,"abstract":"<p><strong>Objectives: </strong>This work was carried out with a view to determining the antioxidant, anti-inflammatory and anti-ulcer properties of the aqueous lyophilized extract of <i>Markhamia lutea</i>.</p><p><strong>Methods: </strong><i>In vitro</i> proteinases inhibition, albumin denaturation, hemolysis of red blood cells by heat, inhibition of the proton pump H⁺/K⁺ATPase, FRAP (Ferric reducing antioxidant power) and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays were performed. <i>In vivo</i>, cold water immersion-induced ulceration and methylene blue-induced ulceration was used to determine the anti-ulcer properties of the lyophilizate (100, 200 and 300 mg/kg).</p><p><strong>Results: </strong><i>In vitro</i>, the lyophilizate (400 μg/mL) significantly inhibited protein denaturation (66.65 %), hemolysis of red blood cells (56.54 %), proteinase activity (69.22 %); then the IC₅₀ was 26.31 μg/mL on proton pump activity. It has also developed a strong ferric reducing antioxidant power (EC₅₀=52.96 mmol FeSO₄/g) as well as free radicals scavenging activity (EC₅₀=22.38 μg/mL). <i>In vivo</i>, the aqueous lyophilizate (200 and 300 mg/kg) protected the gastric mucosa (70.68 and 79.00 % protection respectively) and reduced (p<0.05) acetylcholine, calcium and corticosterone concentrations. A decrease in malondialdehyde level, an increased glutathione level and an increased in catalase and SOD activities were recorded. In the methylene blue test, it significantly increased gastric fluid pH, while reducing gastric volume and improving hematological parameters in ulcer animals. In addition, the histological sections show that the aqueous lyophilizate of <i>M. lutea</i> protected the gastric mucosa from the deleterious effects of stress.</p><p><strong>Conclusions: </strong>The aqueous lyophilizate of <i>M. lutea</i> has anti-ulcer properties thanks to its anti-inflammatory, antioxidant and anti-secretory properties.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"248-257"},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the phyto-restorative potential of ethereal distillates for atopic dermatitis: an advanced therapeutic approach.","authors":"Ritchu Babbar, Parth Sharma, Rashmi Arora, Twinkle Sharma, Madhukar Garg, Sumitra Singh, Satyender Kumar, Rakesh K Sindhu","doi":"10.1515/jcim-2024-0103","DOIUrl":"10.1515/jcim-2024-0103","url":null,"abstract":"<p><p>Atopic dermatitis is acknowledged as a vital inflammatory disorder associated with the integumentary system of the body and is characterized by the formation of thick reddish-grey scars and erythema formation on skin, prevalent amidst the populace. Numerous synthetic drugs are available for treatment like antihistamines, immunosuppressants, glucocorticoids etc., but contrarily, essential oil therapy is exclusively lime lighted to favour the purpose. The utilization of available engineered drugs, possess the marked adverse effects owing to prolonged duration of therapy and therefore, essential oils are explored well and proved to exhibit the anti-eczematic, anti-inflammatory and antipruritic properties. Ethereal distillates own the assorted and selective therapeutic properties attributable to presence of bioactive compounds liable to treat this torturous and integumentary disorder, likely lavender oil, patchouli oil, frankincense oil etc., have been found to exert their pharmacological actions by impeding the liberation and action of inflammatory mediators and immunological hyperactivities that are engaged in exacerbating this idiopathic illness. The current attempt provided the update with the aim to bring forth the naturally originated treatment that is pertinent to provide the invulnerable therapy by circumventing the noxious symptoms i.e. erythema formation and inflamed lesions.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"213-227"},"PeriodicalIF":0.0,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140856446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of <i>Laghoo Shankaprakshalana</i> (yogic colon cleansing) on anthropometric measurements in obese individuals.","authors":"Gayathri Annamalai, Kumaresan Poornachandran, Naveena Arumugam, Prabu Poornachandran, Velan Arumugam, Sankaralingam Thirupathy Venkateswaran","doi":"10.1515/jcim-2024-0048","DOIUrl":"10.1515/jcim-2024-0048","url":null,"abstract":"<p><strong>Objectives: </strong>Obesity is considered as one of the major public health burden and it is predictor of cardiovascular risk factor. Yoga is a mind-body medicine helps to promote health. This study was aimed to explore whether <i>Laghoo Shankaprashalana</i> (LS) reduce the body weight in obese individuals.</p><p><strong>Methods: </strong>This was a single group pre-post study. A total of 40 subjects (9 Male, 31 Female) aged between 18 and 40 years were recruited. This study was conducted in government yoga and naturopathy medical college, Chennai. Subjects were practiced weekly once LS for eight weeks (eight sessions). Subjects were assessed pre and post on body weight, body mass index, waist circumference, hip circumference, waist hip ratio, pulse rate and blood pressure. Variables were analyzed using Paired sample t test except waist hip ratio, which were analyzed using Wilcoxon's signed ranks test.</p><p><strong>Results: </strong>There was significant reduction in the body weight (p<0.001, 5.81 %), Body Mass Index (p<0.001, 5.88 %), Waist Circumference (p<0.001, 7.20 %), Hip Circumference (p<0.001, 5.04 %), and Waist Hip Circumference (p<0.001, 3.19 %) after LS. However, there was no statistical significance difference in pulse rate, blood pressure.</p><p><strong>Conclusions: </strong>Eight sessions of Laghoo Shankaprakshalana helps to reduces body weight, body mass index, waist circumference, hip circumference and waist hip ratio. Reduction in the waist circumference might helps to reduce the cardiovascular risk. LS may be one of the cost effective intervention that can be incorporated in the weight loss regimen.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"258-263"},"PeriodicalIF":0.0,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140865110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Palm oil amends serum female hormones, ovarian antioxidants, inflammatory markers, and DNA fragmentation in favism-induced female rats.","authors":"Khaled M M Koriem, Mahmoud S S Arbid","doi":"10.1515/jcim-2024-0082","DOIUrl":"10.1515/jcim-2024-0082","url":null,"abstract":"<p><strong>Background: </strong>Favism is a metabolic disease and this study evaluates the effectiveness of palm oil and its triacylglycerol constituent in favism-induced female rats to restore serum female hormones, ovarian antioxidants, inflammatory markers, and DNA fragmentation.</p><p><strong>Methods: </strong>Animals were 36 female albino rats. They classified to two equal (normal and favism) groups. The normal group was divided into three equal subgroups: the control, palm oil, and triacylglycerol subgroups. The normal rats were given 1 mL of saline, 1 mL of palm oil, and 1 mL of triacylglycerol orally, respectively. The Favism group was classified also into three equal subgroups: the favism group, the favism + palm oil, the Favism + triacylglycerol. The favism rats were given 1 mL of saline, 1 mL of palm oil, and 1 mL of triacylglycerol orally. For four weeks, all treatments were administered orally via oral gavage once daily.</p><p><strong>Results: </strong>The hemoglobin, hematocrite, the blood cells, glucose and glucose-6-phosphate dehydrogenase, and liver function were decreased in favism. Female hormones such as serum luteinizing hormone, follicle stimulating hormone, Estrone, Estriol, 17α-Estradiol, 17β-Estradiol, and Estradiol-17-β-stearate were decreased in favism. Ovarian antioxidants were decreased while ovarian inflammatory markers were increased in favism. Favism induced ovarian DNA apoptosis. Furthermore, oral administration with palm oil or its triacylglycerol constituent in favism-induced female rats restored all these parameters to be approached the control levels.</p><p><strong>Conclusions: </strong>Palm oil restored serum female hormones, ovarian antioxidants, inflammatory markers, and DNA fragmentation in favism-induced female rats and this effect related to oil triacylglycerol constituent.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"331-339"},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140863009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A short review on deciphering <i>Vyadhikshamatva</i>: insights from HLA-B27-ankylosing spondylitis relation.","authors":"Athul T P, Meena S Deogade, Tanuja M Nesari, Athira Ramachandran","doi":"10.1515/jcim-2024-0083","DOIUrl":"10.1515/jcim-2024-0083","url":null,"abstract":"<p><strong>Objectives: </strong>Various aspects of the concept of Vyadhikshamatva have been thoroughly explored, highlighting its profound significance in resisting disease manifestation, particularly in the context of Ankylosing spondylitis. Investigated the relationship between HLA-B27 and Ankylosing spondylitis (AS) by examining current knowledge and hypotheses Furthermore, efforts were made to portray the influence of <i>prakruti</i> (constitution) and <i>balam</i> (strength) on disease manifestation and progression.</p><p><strong>Methods: </strong>Ayurvedic literature along with contemporary research works was analyzed for correlating various aspects like <i>vyadhikshamatva,oja</i> (The final essence of all body elements), and <i>balam</i> along with their influence on the defensive mechanism of the body. A thorough literature search was conducted to explore the strong association between HLA-B27 and AS by examining various hypotheses like the Arthritogenic peptide hypothesis, the Misfolding hypothesis, the Surface Homodimer hypothesis, and the β2 microglobulin hypothesis that attempts to explain the pathogenic role of HLA-B27 in AS. Alongside classical Ayurvedic texts, databases like PubMed and Scopus were searched using keywords such as Immunity, Ankylosing spondylitis, Vyadhikshamatva, HLA-B27, <i>Balam</i>, and Autoimmune disorder with the help of Boolean operators.</p><p><strong>Results: </strong>The review highlighted the critical role of <i>Vyadhikshamatva</i> in disease prevention, particularly in influencing the manifestation of conditions like AS despite genetic predisposition (HLA-B27). Further, the understanding of the Ayurvedic concepts can clearly explain the conflict that has arisen in the determination of the positive HLAB27 gene in Ankylosing Spondylitis as a definite diagnosing criteria.</p><p><strong>Conclusions: </strong>This comprehensive understanding will uplift the need for personalized medicine in disease management. Further research must be needed to understand the interaction between genetic factors (HLAb27), individual constitution, and their <i>vyadikshamatva</i>.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"264-267"},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140859365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A preclinical study to determine the anti-epileptic effect of biotin on maximal electroshock (MES) and pentylenetetrazole (PTZ) models in albino rats.","authors":"Vrinda Vashishtha, Amrita Parida, Mohan Babu Amberkar, Mohandas Rao K G, Geethika Y, Arpita Roy","doi":"10.1515/jcim-2024-0021","DOIUrl":"10.1515/jcim-2024-0021","url":null,"abstract":"<p><strong>Objectives: </strong>Vitamin B7(biotin) is not synthesized in our body and is retrieved from some food products like eggs, liver, pork and leafy vegetables and as well as microbes of gut. Deficiency of biotin majorly leads to loss of hair, rashes over skin, lethargy and seizures. It is noted that biotin is an anti-oxidant and negates free radical effects. Biotin is also involved in carbon dioxide metabolism and it might alter seizure threshold. Studies also suggest its effect on lipid metabolism as well. So, the primary objective of this study was to assess the efficacy of biotin in maximal electric shock (MES) induced generalized tonic-clonic seizures (GTCS) and pentylenetetrazole (PTZ) induced absence seizures. The secondary objective is to study the effect of combined treatment of biotin and sodium valproate on seizures as well as plasma lipid profile in rats.</p><p><strong>Methods: </strong>In our study 30 albino Wistar rats each were used in MES and PTZ model respectively. 30 rats were divided equally into following groups: I - distilled water (negative control) II - distilled water (positive control) III - sodium valproate (300 mg/kg) IV - biotin (10 mg/kg/day) V - biotin (10 mg/kg) + sodium valproate (150 mg/kg).</p><p><strong>Results: </strong>We observed that the tonic hind limb extension was significantly reduced in the treatment group in MES model. Nitric oxide levels were also seen raised in combination group in MES model and all the treated groups in PTZ model. Biotin treated group showed increased high-density lipoproteins and reduced low density lipoproteins and triglycerides.</p><p><strong>Conclusions: </strong>Biotin had an additive effect to sodium valproate in both the models of epilepsy in rats. Further, it was also able to counteract hyperlipidemia cause by sodium valproate.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"222-229"},"PeriodicalIF":0.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140866470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethanolic extract of <i>Persea americana</i> Mill. (Lauraceae) seeds induced antiestrogenic effects in young female Wistar rats.","authors":"Stéphane Minko Essono, Marie Alfrede Mvondo, Marius Trésor Kamgaing Wego, François Xavier Kemka Nguimatio, Aimé Césaire Momo Tetsatsi, Pierre Watcho","doi":"10.1515/jcim-2023-0218","DOIUrl":"10.1515/jcim-2023-0218","url":null,"abstract":"<p><strong>Objectives: </strong>The ethanol extract of <i>Persea americana</i> seeds was found to inhibit the development of estrogen-dependent conditions in female Wistar rats, suggesting the ability of its secondary metabolites to interact with estrogen receptors (ERs), either as partial agonists or as antagonists. To test this hypothesis, the abovementioned extract was assessed for its ability to mimic and/or antagonize estradiol effects.</p><p><strong>Methods: </strong>Two experiments were conducted in ovariectomized (OVX) rats: (1) animals were treated with estradiol valerate (E₂V; 1 mg/kg) or <i>P. americana</i> at doses of 25 and 50 mg/kg; (2) animals were treated with E₂V alone (0.75 mg/kg) or in combination with <i>P. americana</i> at the abovementioned doses. Treatments were given orally for 3 days and animals were sacrificed for biochemical and histological analyses of the uterus and vagina.</p><p><strong>Results: </strong>When administered alone, <i>P. americana</i> did not change the histomorphology of both organs (uterus and vagina). In combination with E₂V, <i>P. americana</i> decreased uterine weight [30 % decrease (p<0.001) at 25 mg/kg and 24 % (p<0.01) at 50 mg/kg] and epithelium height (37 % decrease). This was associated with decreased estradiol levels (at least 86 % decrease, p<0.001) in the uterus. Similarly, vagina epithelium height decreased by at least 34 % (p<0.05) when E₂V was co-administered with <i>P. americana</i>.</p><p><strong>Conclusions: </strong>The seed extract of <i>P. americana</i> contains ER antagonist secondary metabolites accounting for its ability to inhibit the development of estrogen-dependent conditions in female rats.</p>","PeriodicalId":15556,"journal":{"name":"Journal of Complementary and Integrative Medicine","volume":" ","pages":"175-183"},"PeriodicalIF":0.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140874666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}