Journal of Chromatography & Separation Techniques最新文献_第3页

Adsorption of Phenolic Compounds from an Aqueous By-product of Sunflower Protein Extraction/Purification by Macroporous Resins 大孔树脂对向日葵蛋白萃取纯化副产物中酚类化合物的吸附

Journal of Chromatography & Separation Techniques Pub Date : 2020-01-01 DOI: 10.35248/2157-7064.20.11.435

Tuong Vy. Le Thi, Arnaud Aymes, X. Framboisier, I. Ioannou, R. Kapel

{"title":"Adsorption of Phenolic Compounds from an Aqueous By-product of Sunflower Protein Extraction/Purification by Macroporous Resins","authors":"Tuong Vy. Le Thi, Arnaud Aymes, X. Framboisier, I. Ioannou, R. Kapel","doi":"10.35248/2157-7064.20.11.435","DOIUrl":"https://doi.org/10.35248/2157-7064.20.11.435","url":null,"abstract":"In this study, adsorption of phenolic compounds from an aqueous by-product of sunflower protein isolate production was investigated. Phenolic compounds in this by-product (ultrafiltration permeate of protein purification step) were almost exclusively CGA (mainly 5-CQA isomer). Five different macroporous resins including XAD4, XAD7, XAD16, XAD1180, and HP20 were screened for CGA capture. XAD16 had the best massic adsorption capacities (15.32 ± 0.04 mg/g), while XAD7 had a better surface adsorption capacity (0.027 ± 0.00146 mg/m²). CGA adsorption on both resins followed the pseudo-second-order kinetic model with a similar intra diffusional pattern. Adsorption isotherms of the two resins better fitted the Langmuir model with Qmax for XAD7 and XAD16 of 0.054 and 0.040 (mg/m²), respectively. The adsorption process of phenolic compounds revealed to be exothermic, physical adsorption, and spontaneous. Better adsorption results were observed at 25°C. Maximal CGA desorption ratio was observed from 70% (v/v) ethanol. The high values were reached with both the resins (88.09 ± 0.13 and 86.16 ± 0.32% for XAD7 and XAD16, respectively). The CGA purity in the desorption phase was surprisingly high (77.56 ± 0.99% and 74.59 ± 0.12% for XAD7 and XAD16, respectively).","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"4 1","pages":"435"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81662293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

A Rapid LC-MS/MS Method for Quantification of Acetaminophen in Human Whole Blood LC-MS/MS快速定量测定人全血中对乙酰氨基酚含量

Journal of Chromatography & Separation Techniques Pub Date : 2020-01-01 DOI: 10.35248/2157-7064.20.11.432

K. DivyaVilas, C. eepReddy, R. Prashanthi, C. Ankit

{"title":"A Rapid LC-MS/MS Method for Quantification of Acetaminophen in Human Whole Blood","authors":"K. DivyaVilas, C. eepReddy, R. Prashanthi, C. Ankit","doi":"10.35248/2157-7064.20.11.432","DOIUrl":"https://doi.org/10.35248/2157-7064.20.11.432","url":null,"abstract":"Acetaminophen (paracetamol, N-(4-hydroxyphenyl) acetamide) is one of the most prescribed drugs for the pain management, minor aches, and treatment of fever. Quantification of acetaminophen in pre-term and term neonates and small children requires the availability of highly sensitive assays in small volume blood samples. We developed and validated an LC-MS/MS assay for the quantification of acetaminophen in human whole blood. The drug molecule was extracted from whole blood samples followed by protein precipitation extraction technique followed by LC-MS/MS analysis. An excellent analyte separation was achieved by using a phenomenex Gemini® C18 column (50× 3.0 mm, 3 μm) using mobile phase A-Water:Formic Acid (100:0.1) and Acetonitrile:Formic acid (100:0.1) as mobile phase B. With 3.40 minutes as analytical run time, this analytical method demonstrated linearity between 50.0 to 50,000 ng/mL and the achieved correlation coefficient (r2) was greater than 0.9996. Accuracy, precision, matrix effect and robustness were all acceptable according to FDA guidelines. In addition, acetaminophen was stable up to 179 days at both -20°C and -70°C temperatures. Hence, the acquired results proved that the LC-MS/MS was precise and accurate for quantification of acetaminophen in whole blood, and eventually this method can be applied to micro-sampling technique which is the recent trend towards sample miniaturization, collection of the sample from home and sending the devices for laboratory testing. Keywords: Acetaminophen; Paracetamol; LC-MS/MS; Protein precipitation; Human Whole Blood","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"104 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80862054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development of Validation and Stability Indicating Method of Anti-HIV Dolutegravir Drug and its Related Impurities by Using RP-HPLC 用反相高效液相色谱法建立抗hiv多替格拉韦药物及其相关杂质的验证及稳定性指示方法

Journal of Chromatography & Separation Techniques Pub Date : 2020-01-01 DOI: 10.35248/2157-7064.20.11.426

V. M, Siva Jyothi N

{"title":"Development of Validation and Stability Indicating Method of Anti-HIV Dolutegravir Drug and its Related Impurities by Using RP-HPLC","authors":"V. M, Siva Jyothi N","doi":"10.35248/2157-7064.20.11.426","DOIUrl":"https://doi.org/10.35248/2157-7064.20.11.426","url":null,"abstract":"A simple, rapid and robust reverse phase HPLC method was developed and validated for the determination of impurities in Dolutegravir drug substance. The main aim of this study is to reduce the time consumption and to develop and validate a less expensive method by using HPLC. The chromatographic separation of Dolutegravir and its related impurities is carried out by using C8 column (150 × 4.6 mm), 5µm with 0.1% trifluoroacetic acid in water as mobile phase A, methanol as mobile phase B. The flow rate is 1.0 mL/min with gradient elution mode and the wave length for detection is 240 nm (UV detector). The developed method was validated and proved that the method was specific, accurate and precise as per ICH. The system suitability criteria found to be within the limits. The limit of detection and limit of quantification demonstrate that the method is sensitive. The linearity curve was found to be linear and the correlation coefficient obtained is not less than 0.998. The average percentage recoveries of impurities were in the range of 97 to 101%. The proposed method was found to be suitable and accurate for quantitative determination of impurities in Dolutegravir drug substance.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"25 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89838744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Simultaneous Estimation of Four Preservatives in Pharmaceutical Ointment by RP-HPLC 反相高效液相色谱法同时测定药膏中4种防腐剂的含量

Journal of Chromatography & Separation Techniques Pub Date : 2020-01-01 DOI: 10.35248/2157-7064.20.11.434

L. Alagić-Džambić, M. Dacić, M. Stanić, Omerdic Eo, M. Džambić

引用次数: 1

Quantitation of Paroxetine in Human Plasma by LC-MS/MS: OvercomingSignificant Column-to-Column Retention Time Variation LC-MS/MS定量人血浆中帕罗西汀:克服柱间保留时间的显著变化

Journal of Chromatography & Separation Techniques Pub Date : 2019-04-04 DOI: 10.4172/2157-7064.1000419

J. Chi, Moo-Young Kim, Fumin Li

引用次数: 0

Removal of Cesium from Simulated Spent Fuel Dissolver Liquor 模拟乏燃料溶解液中铯的去除

Journal of Chromatography & Separation Techniques Pub Date : 2019-01-21 DOI: 10.4172/2157-7064.1000417

G. Bond, H. Eccles, Parthiv C. Kavi, Alistair F. Holdsworth, D. Rowbotham, R. Mao

{"title":"Removal of Cesium from Simulated Spent Fuel Dissolver Liquor","authors":"G. Bond, H. Eccles, Parthiv C. Kavi, Alistair F. Holdsworth, D. Rowbotham, R. Mao","doi":"10.4172/2157-7064.1000417","DOIUrl":"https://doi.org/10.4172/2157-7064.1000417","url":null,"abstract":"With a resurgence of the nuclear industry’s fortunes, waste management will be an even greater consideration; \u0000reducing wastes, better segregation and treatment that lower the impact on waste storage facilities and repositories, \u0000in particular geological repositories will help the sustainability of the industry. We at the University of Central \u0000Lancashire (UCLan) proposed in a previous publication a sequential chromatographic separation process, Alternative \u0000Reprocessing Technology (ART) for Fission Products (FPs) and Minor Actinides (MAs) separation from spent \u0000fuel dissolver liquor, as an improvement to/replacement for PUREX (Plutonium Uranium Redox Extraction). This \u0000publication addresses the removal of one particular fission product, cesium, and its impact on waste management, \u0000and down-stream PUREX operations. Although our proposed process is still in its infancy, its impact on the PUREX \u0000process could be significant, with major gains in the separation circuit, waste management of High Level Waste \u0000(HLW) and subsequently waste disposal to and design of a geological repository. This paper briefly describes; our \u0000concept, some preliminary experimental data and why re-classification of the bulk of HLW (High Level Waste) to \u0000Intermediate Level Waste (ILW) would be possible.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"7 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2019-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89757954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Validation of a Rapid Test Method for the Quantification of Priority Anions inDrinking Water by Ion Chromatography-Suppressed Conductivity Detection 离子色谱抑制电导率检测法定量饮用水中优先阴离子的快速试验方法的验证

Journal of Chromatography & Separation Techniques Pub Date : 2019-01-01 DOI: 10.4172/2157-7064.1000418

T. Manickum, Khumalo Tp, S. Qegu

{"title":"Validation of a Rapid Test Method for the Quantification of Priority Anions inDrinking Water by Ion Chromatography-Suppressed Conductivity Detection","authors":"T. Manickum, Khumalo Tp, S. Qegu","doi":"10.4172/2157-7064.1000418","DOIUrl":"https://doi.org/10.4172/2157-7064.1000418","url":null,"abstract":"A simple, rapid (8 min) ion chromatographic test method, using post-column electrolytic suppression, with conductivity detection, is validated for the quantification of Cl-, NO2 -, NO3 -, SO4 2- in river, dam, potable, waste water and in trade effluent, over the linear range: 1-100 mg/L for chloride, 0.05-5 mg/L for nitrite and nitrate, and 0.5-50 mg/L for sulphate. The ion chromatographic separation was carried out using a Dionex Ion PacTM AS22-fast 4 μm column (4 × 150 mm), with a flow rate of 1.2 mL/min, using Na2CO3/NaHCO3 (4.5 mM/1.4 mM) as eluent. The LOQ was in the range 0.025-0.50 mg/L, with an injection volume of 25 μL. The %RSD for retention time and peak area responses was <1%. Spiked recoveries were 87-107% for all 4 target anions. The method was suitable for routine drinking water quality assessment in line with the national regulations. The method was fully validated for accuracy, precision, recovery, LOQ, bias/trueness, selectivity, Measurement Uncertainty (MU), etc. The method validation data complied with the international requirements for testing laboratories for ISO/IEC 17025 accreditation.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89915835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of COL3A1-Specific Real-Time PCR Assay for Clinical and Sport Detection Applications col3a1特异性实时荧光定量PCR在临床和运动检测中的应用

Journal of Chromatography & Separation Techniques Pub Date : 2018-04-25 DOI: 10.4172/2157-7064.1000403

B. Martínez, Á. Jiménez, T. Díaz, J. Segura, P. Ferro

{"title":"Development of COL3A1-Specific Real-Time PCR Assay for Clinical and Sport Detection Applications","authors":"B. Martínez, Á. Jiménez, T. Díaz, J. Segura, P. Ferro","doi":"10.4172/2157-7064.1000403","DOIUrl":"https://doi.org/10.4172/2157-7064.1000403","url":null,"abstract":"PIIINP, whose gene is COL3A1, is a protein used as a biomarker, which is altered in various pathologies such as fatty liver of non-alcoholic origin and is also used to detect abuse of human recombinant growth hormone (rhGH) by elite athletes to improve their performance. Therefore, the study of the levels of this protein or its gene could be useful for the diagnosis of these pathologies, in addition to other uses. The objective of this work was the development of a new detection method of the COL3A1 gene based on the quantification of mRNA in real time. 10 healthy men volunteers were injected intravenously for 3 consecutive days with 0.026 mg-1kg-1day-1person-1 of rhGH, and serum and blood samples were collected for 7 days at different times. mRNA extraction was performed after lymphocyte purification following standard protocols, and samples were quantified by quantitative real-time PCR using TaqMan probes designed specifically for this gene. The results showed an increase in COL3A1 expression that was maintained during 7 days of sampling after the last administration, observing more sensitive and robust results when compared to quantification of the PIIINP protein in serum from the same individuals. After the trial set-up, a partial validation was carried out in which parameters such as linearity, inter and intra-assay variation, influence of sex and age, stability of blood samples storage at 4°C and amount of blood sample, were studied.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"41 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2018-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78972510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Fatty Acid Composition of Muscles Tissues of Longdong Goat (Black and White Cashmere) 陇东山羊(黑白羊绒)肌肉组织脂肪酸组成的研究

Journal of Chromatography & Separation Techniques Pub Date : 2018-04-18 DOI: 10.4172/2157-7064.1000402

Babour Aj, Wei Jm, Lu Yz

{"title":"Fatty Acid Composition of Muscles Tissues of Longdong Goat (Black and White Cashmere)","authors":"Babour Aj, Wei Jm, Lu Yz","doi":"10.4172/2157-7064.1000402","DOIUrl":"https://doi.org/10.4172/2157-7064.1000402","url":null,"abstract":"This study examined fatty acid (FA) composition of muscle tissue of Longdong black goat and Longdong white Cashmere goat breed under reared systems. Approximately 86.31% of fatty acids were observed. Oleic acid was extensively the most abundant ranged from 22.98% to 26.02% of Longdong black and white Cashmere goat respectively. The mean amount of SFA and MUFA were about 21.46% to 34.82% respectively. PUFA were 30.02% of total FA composition. C20:4 were founded 12.14% in all muscles tissues. Compare to C20:5, C22:6 16.43%, 4.88% respectively. The amount of SFA: MUFA ratio was low in Longdong goat muscles tissues. PUFA and MUFA had higher amount female black muscles tissues. Females and males of both had great amount of C16:0, C18:0, ranged from 0.86% to 29.16%. C18:2 were found in 15.01%. The study found fatty acids composition in both Longdong goat breed (black and white cashmere) has some significant effect (P<0:05) in fatty acids proportions. The greater ratios of unsaturated fatty acids MUFA and PUFA high amount than saturated fatty acids SFA in goat muscle tissue and this indicate that Longdong goats breed are healthier and have the same type of fatty acids composition.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"16 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2018-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84754179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enantio Separation of HMB (1-Hydroxy Methylthio Butanoic Acid) through Chymotrypsin Catalysed Peptide Synthesis and Chiral LC-MS Assessment 胰凝乳酶催化合成1-羟基甲基硫丁酸对映体分离及手性LC-MS评价

Journal of Chromatography & Separation Techniques Pub Date : 2018-04-04 DOI: 10.4172/2157-7064.1000400

Santhana Srinivasan, S. Kapila, D. Forciniti, P. Nam

{"title":"Enantio Separation of HMB (1-Hydroxy Methylthio Butanoic Acid) through Chymotrypsin Catalysed Peptide Synthesis and Chiral LC-MS Assessment","authors":"Santhana Srinivasan, S. Kapila, D. Forciniti, P. Nam","doi":"10.4172/2157-7064.1000400","DOIUrl":"https://doi.org/10.4172/2157-7064.1000400","url":null,"abstract":"Alpha hydroxy acids (AHAs) have received wide attention in cosmetic and animal-feed industries. Lactic acid, malic acid and the hydroxy analog of methionine (HMB) are important members of the AHA group. The chemically synthesized formulations of alpha hydroxy acids are a racemic mixture comprising of 50% L and 50% D forms. It is a well-documented fact that enantiomeric forms of chemicals can possess different activities. Enantio purity of chemicals is important in their use in pharmaceutical applications and it may play a significant role in nutritional and cosmetic applications as well. However, this role has not been fully documented because of the lack of enantio pure HMB and other AHAs. In this work, papain and chymotrypsin catalyzed peptide capping was evaluated for obtaining enantio pure alpha hydroxy acids. Chymotrypsin catalyzed peptide capping was found to be a very fast and efficient tool to obtain enantiopure HMB. The enantio purity of products was evaluated with Reverse Phase liquid Chromatography and Chiral Liquid Chromatography-Mass Spectrometry. The results indicate that enantio-purity of greater than 95% was obtained in less than ~30 minutes with chymotrypsin catalyzed capping HMB capping reaction.","PeriodicalId":15534,"journal":{"name":"Journal of Chromatography & Separation Techniques","volume":"27 1","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2018-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81751790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0