Journal of Clinical Microbiology最新文献

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Genomic epidemiology and antimicrobial resistance of Corynebacterium macclintockiae, the predominant species of human pathogens within the Corynebacterium jeikeium complex. 杰氏棒状杆菌复合体中人致病菌的优势种——麦克林托基棒状杆菌的基因组流行病学和耐药性。
IF 5.4 2区 医学
Journal of Clinical Microbiology Pub Date : 2025-08-13 Epub Date: 2025-07-24 DOI: 10.1128/jcm.00500-25
Sohei Harada, Kohji Komori, Kenya Yukawa, Brian Hayama, Kazumi Takehana, Kageto Yamada, Asako Doi, Tomoo Saga, Masakazu Sasaki, Yoshiro Hadano, Masahiro Suzuki, Kyoko Yokota, Jun Suzuki, Koki Kikuchi, Yohei Doi, Kazuhiro Tateda
{"title":"Genomic epidemiology and antimicrobial resistance of <i>Corynebacterium macclintockiae</i>, the predominant species of human pathogens within the <i>Corynebacterium jeikeium</i> complex.","authors":"Sohei Harada, Kohji Komori, Kenya Yukawa, Brian Hayama, Kazumi Takehana, Kageto Yamada, Asako Doi, Tomoo Saga, Masakazu Sasaki, Yoshiro Hadano, Masahiro Suzuki, Kyoko Yokota, Jun Suzuki, Koki Kikuchi, Yohei Doi, Kazuhiro Tateda","doi":"10.1128/jcm.00500-25","DOIUrl":"10.1128/jcm.00500-25","url":null,"abstract":"<p><p>Genomic characteristics and optimal treatment of <i>Corynebacterium jeikeium</i> remain largely unknown. We collected clinical information and performed whole-genome sequencing analysis of the causative strains of six cases of <i>C. jeikeium</i> infection at a single hospital over a 9-year period. Additionally, whole-genome sequencing analysis was performed on 33 <i>C</i>. <i>jeikeium</i> strains from cases of bloodstream infection at eight hospitals. Antimicrobial susceptibility testing was performed, and the results were compared to the resistance genes identified. Publicly available genome data of strains of <i>C. jeikeium</i> complex, consisting of <i>C. jeikeium sensu stricto</i>, <i>Corynebacterium macclintockiae</i>, and <i>Corynebacterium evansiae</i>, worldwide, were combined with the data from this study to determine the distribution of genomic species. In the single-center study, cases of prosthetic osteoarticular infection, postoperative intra-abdominal infection, and catheter-related bloodstream infection were identified, and the causative strains were genomically identified as <i>C. macclintockiae</i>. All but one isolate (32/33, 97.0%) in the eight-center study identified as <i>C. jeikeium</i> by matrix-assisted laser desorption ionization-time of flight mass spectrometry were also genomically identified as <i>C. macclintockiae</i>. Nosocomial transmission was suggested in three strain pairs by core-genome single nucleotide polymorphism analysis. <i>C. macclintockiae</i> strains were generally multidrug-resistant, but all anti-methicillin-resistant <i>Staphylococcus aureus</i> agents, including teicoplanin, had favorable activity, and the strains without the <i>tet</i>(W) gene (22/38, 57.9%) were susceptible to tetracyclines. Genome analysis of 66 <i>C</i>. <i>jeikeium</i> complex strains collected worldwide, consisting mainly of clinical strains, re-identified 51 strains (77.3%) as <i>C. macclintockiae</i>. This study demonstrates that <i>C. macclintockiae</i> is the major genomic species of the <i>C. jeikeium</i> complex causing human infections.IMPORTANCERecent widespread use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has facilitated the identification of <i>Corynebacterium</i> spp. in microbiology laboratories, thereby raising awareness of the clinical importance of these organisms. Nevertheless, the accumulation of information on genomic characteristics of <i>Corynebacterium jeikeium</i> has been significantly limited compared to other pathogenic organisms thus far. In this study, we analyzed causative strains of infections identified as <i>C. jeikeium</i> by MALDI-TOF MS, collected from multiple institutions throughout Japan, and found that most of these strains were genomically identified as <i>Corynebacterium macclintockiae</i>, a species that has been newly described recently. Collection of clinical information on selected cases showed that <i>C. macclintockiae</i> ","PeriodicalId":15511,"journal":{"name":"Journal of Clinical Microbiology","volume":" ","pages":"e0050025"},"PeriodicalIF":5.4,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12345161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An ELISA-like sensitive and visual detection system targeting Yersinia pestis based on CRISPR/Cas12a and DNAzyme. 基于CRISPR/Cas12a和DNAzyme的鼠疫耶尔森氏菌elisa样灵敏视觉检测系统
IF 5.4 2区 医学
Journal of Clinical Microbiology Pub Date : 2025-08-13 Epub Date: 2025-07-24 DOI: 10.1128/jcm.00274-25
Yingqing Mao, Ruichen Lv, Hao Shao, Yong Zhao, Junhu Wang, Qiong Chen, Haiming Yi, Yixin Ge, Hongming Wang, Yuexi Li, Yong Qi
{"title":"An ELISA-like sensitive and visual detection system targeting <i>Yersinia pestis</i> based on CRISPR/Cas12a and DNAzyme.","authors":"Yingqing Mao, Ruichen Lv, Hao Shao, Yong Zhao, Junhu Wang, Qiong Chen, Haiming Yi, Yixin Ge, Hongming Wang, Yuexi Li, Yong Qi","doi":"10.1128/jcm.00274-25","DOIUrl":"10.1128/jcm.00274-25","url":null,"abstract":"<p><p><i>Yersinia pestis</i> is the causative agent of plague, a human disease with potentially devastating consequences. Here, we developed an enzyme-linked immunosorbent assay-like visual detection method based on clustered regularly interspaced short palindromic repeats (CRISPR) detection and DNAzyme for the cost-effective and highly sensitive detection of <i>Y. pestis</i>. A novel specific gene sequence (CH57_3927) was screened for the detection target of <i>Y. pestis</i>. The recombinase-aided amplification (RAA) assay, CRISPR/Cas12a detection assay, and G-quadruplex (G4) DNAzyme-based color development assay were separately established and optimized. These three optimized assays were integrated into an advanced ELISA-like visual detection method-RAA-CRISPR/Cas12a-DNAzyme (RCCD)-by further optimization of their components to improve the compatibility between them. The amplified target sequence binds to crRNA and activates the Cas12a nucleases for trans-cleave G4. As a result, the cleaved G4 is unable to bind with hemin to exert peroxidase activity, thus impeding the catalysis of the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS<sup>2-</sup>) colorimetric reaction. Consequently, negative samples exhibit a dark green coloration, while the positive products appear nearly colorless, facilitating visual differentiation with the naked eye. In addition, the RCCD detection platform effectively distinguished <i>Y. pestis</i> from all other closely related species, with a detection limit of 1 copy/reaction. Evaluated using <i>Y. pestis</i> DNA-spiked blood samples and uninfected samples, both sensitivity and specificity were 100%. The method shows significant potential for detecting targets in clinical samples and is well-suited for use in resource-limited environments. It offers advantages such as visual detection, batch detection, and low cost.IMPORTANCEWe utilized Mauve software to screen <i>Yersinia pestis</i> specific genes and integrated CRISPR-Cas12a, RAA amplification, and G-quadruplex DNAzyme technology to establish an advanced ELISA-like visual detection method. The visual detection method offers a more cost-effective alternative compared to the conventional CRISPR detection method that relies on fluorescence-labeled ssDNA reporter or lateral flow (LF) test strips. With only one thermostatic device required, it enhances the convenience of rapid on-site screening of <i>Y. pestis</i> outbreaks, providing effective support for plague detection, prevention, and control within primary medical and health institutions.</p>","PeriodicalId":15511,"journal":{"name":"Journal of Clinical Microbiology","volume":" ","pages":"e0027425"},"PeriodicalIF":5.4,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12345247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of BIOBALL and EZ-Accu Shot for accurate quantification as certified quality control reference material. BIOBALL和EZ-Accu Shot作为认证的质量控制参考物质进行精确定量的比较。
IF 5.4 2区 医学
Journal of Clinical Microbiology Pub Date : 2025-08-13 Epub Date: 2025-07-01 DOI: 10.1128/jcm.00520-25
Dustin A Baird, James E T Gebo, Anna F Lau
{"title":"Comparison of BIOBALL and EZ-Accu Shot for accurate quantification as certified quality control reference material.","authors":"Dustin A Baird, James E T Gebo, Anna F Lau","doi":"10.1128/jcm.00520-25","DOIUrl":"10.1128/jcm.00520-25","url":null,"abstract":"","PeriodicalId":15511,"journal":{"name":"Journal of Clinical Microbiology","volume":" ","pages":"e0052025"},"PeriodicalIF":5.4,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12345222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid tests as a practical alternative to slide agglutination for the confirmation of V. cholerae O1. 快速检测作为一种实际替代玻片凝集法用于确认霍乱弧菌O1。
IF 5.4 2区 医学
Journal of Clinical Microbiology Pub Date : 2025-08-13 Epub Date: 2025-07-17 DOI: 10.1128/jcm.00433-25
Piyash Bhattacharjee, Sonia T Hegde, Ashraful Islam Khan, Imrul Kayes Nabil, Md Naiem Hossain, Tahira Ahmed Rashmi, Mokibul Hassan Afrad, Md Taufiqul Islam, Mohammad Ashraful Amin, Zahid Hasan Khan, Taufiqur Rahman Bhuiyan, Andrew S Azman, Firdausi Qadri
{"title":"Rapid tests as a practical alternative to slide agglutination for the confirmation of <i>V. cholerae</i> O1.","authors":"Piyash Bhattacharjee, Sonia T Hegde, Ashraful Islam Khan, Imrul Kayes Nabil, Md Naiem Hossain, Tahira Ahmed Rashmi, Mokibul Hassan Afrad, Md Taufiqul Islam, Mohammad Ashraful Amin, Zahid Hasan Khan, Taufiqur Rahman Bhuiyan, Andrew S Azman, Firdausi Qadri","doi":"10.1128/jcm.00433-25","DOIUrl":"10.1128/jcm.00433-25","url":null,"abstract":"<p><p>Slide agglutination for serogroup and/or serotype identification is a crucial step for confirming cholera by culture. Rapid diagnostic tests (RDTs) are typically used directly on stool but are not considered sufficient for cholera confirmation. However, they may provide a practical alternative to the slide agglutination step of culture<i>,</i> as they are easy to use, store, and require minimal training. This study evaluates the concordance of <i>Vibrio cholerae</i> O1/O139 detection from presumptive colonies by slide agglutination with RDTs. Patients (≥1 year) with acute watery diarrhea at the icddr,b Dhaka hospital were enrolled. Stool samples were cultured on thiosulfate-citrate-bile salts-sucrose medium, and presumptive colonies were subcultured on Gelatin Agar medium. Isolates were then tested by slide agglutination and four commercial cholera RDTs. From 4 February 2024 through 24 May 2025, 1,638 patients with acute watery diarrhea were enrolled, 1,140 (70%) had presumptive <i>V. cholerae</i> colonies, and 482 (29%) were culture-confirmed as <i>V. cholerae</i> O1. We tested suspected <i>V. cholerae</i> colonies using Cholkit (n=1,140), SD Bioline (n=693), Crystal VC O1/O139 (n=693), and Crystal VC O1 (n=655) RDT kits. RDTs showed near-perfect concordance with slide agglutination. Using slide agglutination as the reference, the sensitivity of the RDT kits ranged from 99.3% to 99.6% and the specificity from 99.3% to 99.7%. RDTs offer a practical and potentially easier alternative to slide agglutination of presumptive <i>V. cholerae</i> colonies within typical cholera culture protocols. This may help to provide a pathway to quick confirmation of outbreaks in settings where lab facilities and reagents may be limited.IMPORTANCEThis study demonstrated that rapid diagnostic tests (RDTs) can effectively replace traditional slide agglutination methods to confirm suspected <i>Vibrio cholerae</i> colonies belonging to the O1 serogroup, which are responsible for the current seventh cholera pandemic. Comparing four RDTs to slide agglutination, we found almost perfect agreement, with sensitivity and specificity exceeding 99%. Since RDTs are cheaper, easier to use and store, and require less technical training, adopting them could significantly speed up cholera outbreak confirmation, especially in areas with limited laboratory resources. This practical shift could lead to faster responses to cholera outbreaks, ultimately improving public health surveillance and disease control globally.</p>","PeriodicalId":15511,"journal":{"name":"Journal of Clinical Microbiology","volume":" ","pages":"e0043325"},"PeriodicalIF":5.4,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12345242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multicenter evaluation of blood culture contamination and blood cultures practices in US acute care hospitals: time for standardization. 美国急症医院血液培养污染和血液培养实践的多中心评估:标准化的时间。
IF 5.4 2区 医学
Journal of Clinical Microbiology Pub Date : 2025-08-13 Epub Date: 2025-07-11 DOI: 10.1128/jcm.00530-25
Valeria Fabre, Yea-Jen Hsu, Karen C Carroll, Aaron M Milstone, Alejandra B Salinas, Lilian M Abbo, Chris Bower, Jennifer Berry, Sarah Boyd, Kathleen O Degnan, Pragya Dhaubhadel, Daniel J Diekema, Marci Dress, Baevin Feeser, Mark Fisher, Cynthia Flynn, Bradley A Ford, Erin B Gettler, Laurel J Glasser, Jessica Howard-Anderson, J Kristie Johnson, Sara M Karaba, Justin J Kim, Alyssa Kubischta, Benjamin M Landrum, Marvin Martinez, Amy J Mathers, Leonard Mermel, Rebekah W Moehring, John C O'Horo, Dana E Pepe, S Sonia Qasba, Barry Rittmann, Evan D Robinson, Guillermo Rodríguez-Nava, Rossana Rosa, Jonathan H Ryder, Jorge L Salinas, Aditya Shah, Gregory M Schrank, Mark Shelly, Emily S Spivak, Kathleen O Stewart, Thomas R Talbot, Trevor C Van Schooneveld, Anastasia Wasylyshyn, Avinash Gadala, Zunaira Virk, Sara E Cosgrove
{"title":"Multicenter evaluation of blood culture contamination and blood cultures practices in US acute care hospitals: time for standardization.","authors":"Valeria Fabre, Yea-Jen Hsu, Karen C Carroll, Aaron M Milstone, Alejandra B Salinas, Lilian M Abbo, Chris Bower, Jennifer Berry, Sarah Boyd, Kathleen O Degnan, Pragya Dhaubhadel, Daniel J Diekema, Marci Dress, Baevin Feeser, Mark Fisher, Cynthia Flynn, Bradley A Ford, Erin B Gettler, Laurel J Glasser, Jessica Howard-Anderson, J Kristie Johnson, Sara M Karaba, Justin J Kim, Alyssa Kubischta, Benjamin M Landrum, Marvin Martinez, Amy J Mathers, Leonard Mermel, Rebekah W Moehring, John C O'Horo, Dana E Pepe, S Sonia Qasba, Barry Rittmann, Evan D Robinson, Guillermo Rodríguez-Nava, Rossana Rosa, Jonathan H Ryder, Jorge L Salinas, Aditya Shah, Gregory M Schrank, Mark Shelly, Emily S Spivak, Kathleen O Stewart, Thomas R Talbot, Trevor C Van Schooneveld, Anastasia Wasylyshyn, Avinash Gadala, Zunaira Virk, Sara E Cosgrove","doi":"10.1128/jcm.00530-25","DOIUrl":"10.1128/jcm.00530-25","url":null,"abstract":"<p><p>Clinical and Laboratory Standards Institute (CLSI) recommends a blood culture contamination (BCC) threshold of <3%, with ≤1% considered optimal. However, there is not a standardized definition of BCC, and the effect of multiple definitions on BCC rates or what definitions laboratories use remain unknown. We surveyed 52 hospitals and analyzed 362,078 blood cultures (BCx) collected 1 September 2019 to 31 August 2021 from 62 intensive care units (ICUs) and 231 wards from 48 of these hospitals. We calculated and compared BCC rates using the College of American Pathologists (CAP) or CLSI criteria (both utilize a limited number of skin commensals to define BCC) and the comprehensive National Healthcare Safety Network (NHSN) commensal list. We characterized factors associated with BCC and related outcomes (central-line associated bloodstream infection [CLABSI] and vancomycin use). BCC, BCx positivity, and single BCx rates were monitored by 100%, 39%, and 21% of hospitals, respectively. Hospitals used CAP (65%), CLSI (17%), and NHSN (17%) criteria to define BCC. Mean BCC rate by CAP (CAP-BCC) was 1.38% for ICUs and 0.96% for wards. BCC rates remained similar by CLSI criteria but increased when using NHSN list. Sharing BCC data outside of the laboratory, measuring additional BCx quality indicators, and limiting central catheter-drawn BCx were associated with lower BCC rates. BCC was associated with higher CLABSI rates in ICUs. This study demonstrated variability in laboratory practices and opportunities to optimize BCx stewardship.IMPORTANCEBlood culture contamination (BCC) is associated with patient harm and unnecessary use of healthcare resources. BCC thresholds have been established; however, multiple BCC definitions exist. There is limited data on how BCC rates differ depending on the BCC definition used, what definitions laboratories most commonly use, or their approach to other blood cultures (BCx) quality indicators such as single rates or BCx positivity. A cross-sectional multicenter survey and analysis of BCx data from intensive care unit and wards revealed that most laboratories did not track single BCx or BCx positivity rates and that there was variability in how BCC was defined. Additionally, BCC rates were influenced by the definition used. BCC was associated with increased central-line associated bloodstream infection rates.</p>","PeriodicalId":15511,"journal":{"name":"Journal of Clinical Microbiology","volume":" ","pages":"e0053025"},"PeriodicalIF":5.4,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12345234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A multicenter study to assess the performance of the point-of-care RT-PCR Cobas SARS-CoV-2 & Influenza A/B nucleic acid test for use on the Cobas Liat system in comparison with centralized assays across healthcare facilities in the United States. 一项多中心研究,旨在评估在Cobas Liat系统上使用的即时即时RT-PCR Cobas SARS-CoV-2和流感A/B核酸检测的性能,并与美国各医疗机构的集中检测进行比较。
IF 5.4 2区 医学
Journal of Clinical Microbiology Pub Date : 2025-08-13 Epub Date: 2025-07-02 DOI: 10.1128/jcm.01459-24
Elissa M Robbins, Rasa Bertuzis, Ho-Chen Chiu, Lupe Miller, Christopher Noutsios
{"title":"A multicenter study to assess the performance of the point-of-care RT-PCR Cobas SARS-CoV-2 & Influenza A/B nucleic acid test for use on the Cobas Liat system in comparison with centralized assays across healthcare facilities in the United States.","authors":"Elissa M Robbins, Rasa Bertuzis, Ho-Chen Chiu, Lupe Miller, Christopher Noutsios","doi":"10.1128/jcm.01459-24","DOIUrl":"10.1128/jcm.01459-24","url":null,"abstract":"<p><p>Respiratory diseases can share many of the same symptoms, highlighting the need for timely and accurate differentiation to facilitate effective clinical management and reduce transmission. Compared with centralized testing, molecular point-of-care tests (POCTs) can provide a faster time to result. We evaluated the RT-PCR POCT Cobas® SARS-CoV-2 & Influenza A/B qualitative assay for use on the Cobas Liat system (the POC SARS-CoV-2 & Influenza A/B test) in nasal and nasopharyngeal swab samples from 10 diverse healthcare facilities in the United States. A composite comparator design consisting of three centralized tests was used to analyze severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), while performance vs a single centralized test was used for analysis of influenza A and B. Evaluations included performance stratified by sample type (prospective/retrospective and nasal/nasopharyngeal [paired by subject]), collection method (self/healthcare worker-collected [alternated and approximately balanced]), symptom status (symptomatic/asymptomatic), and SARS-CoV-2 vaccination status, as well as assay inclusivity and system ease of use. A total of 2,247 samples were tested. For SARS-CoV-2, the overall percent agreement (OPA) was 98.8% (95% confidence interval [CI]: 97.9, 99.3) in nasal swab samples and 99.0% (95% CI: 98.2, 99.4) in nasopharyngeal swab samples. Regression analysis showed that cycle threshold values from paired nasal and nasopharyngeal swab samples were highly correlated (correlation coefficient 0.83). The OPA was ≥99.5% (sample type dependent) and 100.0% for influenza A and B, respectively. The POC SARS-CoV-2 & Influenza A/B test was easy to use. These results support the use of the POCT in various sample types and by various operators in the intended-use setting.</p><p><strong>Importance: </strong>This study highlights the benefits of RT-PCR point-of-care tests, namely comparable performance to centralized testing in multiple sample types and ease of use. Utilizing assays such as the POC Cobas SARS-CoV-2 & Influenza A/B test may improve the timely differentiation of respiratory diseases that share similar symptoms.</p>","PeriodicalId":15511,"journal":{"name":"Journal of Clinical Microbiology","volume":" ","pages":"e0145924"},"PeriodicalIF":5.4,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12345166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Staphylococcus aureus complex: implications for the clinical microbiology laboratory. 金黄色葡萄球菌复合体:对临床微生物实验室的启示。
IF 6.1 2区 医学
Journal of Clinical Microbiology Pub Date : 2025-07-09 Epub Date: 2025-06-04 DOI: 10.1128/jcm.01276-24
Austin Yan, Julianne V Kus, Nadia Sant
{"title":"The <i>Staphylococcus aureus</i> complex: implications for the clinical microbiology laboratory.","authors":"Austin Yan, Julianne V Kus, Nadia Sant","doi":"10.1128/jcm.01276-24","DOIUrl":"10.1128/jcm.01276-24","url":null,"abstract":"<p><p>In the last 15 years, advances in diagnostic microbiology have enabled more detailed characterization of human bacterial pathogens. These changes have led to the description of new species within the <i>Staphylococcus aureus</i> complex, including <i>S. aureus</i> subspecies <i>anaerobius</i>, <i>Staphylococcus argenteus</i>, and <i>Staphylococcus schweitzeri</i>. In this minireview, we discuss the history, epidemiology, microbiology, genomics, and clinical significance of the <i>S. aureus</i> complex and provide a guide for laboratories to reliably detect and differentiate these novel species.</p>","PeriodicalId":15511,"journal":{"name":"Journal of Clinical Microbiology","volume":" ","pages":"e0127624"},"PeriodicalIF":6.1,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12239731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Non-fungal pathogens detected by broad-range fungal polymerase chain reaction. 广谱真菌聚合酶链反应检测非真菌病原体。
IF 6.1 2区 医学
Journal of Clinical Microbiology Pub Date : 2025-07-09 Epub Date: 2025-06-10 DOI: 10.1128/jcm.00087-25
Sonya Ahuja, Joshua A Lieberman
{"title":"Non-fungal pathogens detected by broad-range fungal polymerase chain reaction.","authors":"Sonya Ahuja, Joshua A Lieberman","doi":"10.1128/jcm.00087-25","DOIUrl":"10.1128/jcm.00087-25","url":null,"abstract":"","PeriodicalId":15511,"journal":{"name":"Journal of Clinical Microbiology","volume":" ","pages":"e0008725"},"PeriodicalIF":6.1,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12239715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Performance of the LIAISON PLEX yeast blood culture assay for identifying 16 invasive fungal pathogens in blood cultures. 联络PLEX酵母菌血培养试验在血液培养中鉴定16种侵袭性真菌病原体的性能。
IF 5.4 2区 医学
Journal of Clinical Microbiology Pub Date : 2025-07-09 Epub Date: 2025-06-02 DOI: 10.1128/jcm.00362-25
Christopher L Emery, Neelam Dhiman, Siu-Kei Chow, Gwyn Peterson, Paul Granato, Brian Bernier, Janet Farhang
{"title":"Performance of the LIAISON PLEX yeast blood culture assay for identifying 16 invasive fungal pathogens in blood cultures.","authors":"Christopher L Emery, Neelam Dhiman, Siu-Kei Chow, Gwyn Peterson, Paul Granato, Brian Bernier, Janet Farhang","doi":"10.1128/jcm.00362-25","DOIUrl":"10.1128/jcm.00362-25","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Fungi frequently cause potentially life-threatening bloodstream infections, particularly in immunocompromised and hospitalized individuals. Molecular methods can allow earlier pathogen identification for faster optimization of appropriate therapy. This multisite study evaluated the analytical and clinical performance of the automated multiplex LIAISON PLEX Yeast Blood Culture nucleic acid assay (Luminex Corporation, Northbrook, IL), which detects and identifies 14 &lt;i&gt;Candida&lt;/i&gt; and 2 &lt;i&gt;Cryptococcus&lt;/i&gt; pathogens from positive blood cultures. Samples included 69 prospectively collected specimens, 63 pre-selected samples, and 829 contrived specimens. The assay demonstrated 100% positivity in detecting target fungal pathogens and 0% positivity in negative blood cultures. All 13 tested bottle/matrix types gave 100% detection of six evaluated pathogens. Limits of detection ranged from 7.77 × 10&lt;sup&gt;2&lt;/sup&gt; CFU/mL (&lt;i&gt;Candida famata&lt;/i&gt;) to 2.83 × 10&lt;sup&gt;5&lt;/sup&gt; CFU/mL (&lt;i&gt;Candida albicans&lt;/i&gt;). The assay identified five strains tested for each of the 16 target species, indicating good inclusivity. No cross-reactivity occurred with 40 bacterial strains. Cross-reactivity with 2 of 37 off-target fungi (&lt;i&gt;Candida&lt;/i&gt; [&lt;i&gt;Yarrowia&lt;/i&gt;] &lt;i&gt;deformans&lt;/i&gt; and &lt;i&gt;Candida pseudohaemulonii&lt;/i&gt;) was predicted by &lt;i&gt;in silico&lt;/i&gt; sequence analyses. Low concentrations of on-panel fungi were detectable alongside high concentrations of potential fungal and bacterial confounders. Reproducibility was 100% within-lab and 99.7% across sites. For combined prospective plus pre-selected specimens, sensitivity/positive percent agreement (PPA) was 100% for detected fungal pathogens, and specificity/negative percent agreement (NPA) was 97.6%‒100.0%. Common pathogens in prospective samples were &lt;i&gt;Candida glabrata&lt;/i&gt; (36%) and &lt;i&gt;C. albicans&lt;/i&gt; (25%). Contrived specimen PPA was 100%, and NPA was ≥99.6%. The LIAISON PLEX Yeast Blood Culture assay provides outstanding sensitivity and specificity for rapidly identifying and differentiating fungal pathogens in positive blood culture specimens.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Importance: &lt;/strong&gt;Bloodstream fungal infections have serious risks of illness and death. Optimal treatment requires early pathogen identification so appropriate antifungal therapy can be immediately started. Blood culture is standard for confirming fungal infection but takes several days for results and may not provide detailed pathogen information. The LIAISON PLEX Yeast Blood Culture assay quickly analyzes fungus-positive blood cultures to identify 16 pathogenic fungi, including multiple &lt;i&gt;Candida&lt;/i&gt; spp. that are common causes of bloodstream infection. This study demonstrated a high degree of test accuracy and reliability in rapidly identifying the infective pathogen in blood cultures from 132 patients with demonstrated culture-positive fungal infections. Assay performance was confirmed in 829 contrived samples, whereby samples were spiked with different funga","PeriodicalId":15511,"journal":{"name":"Journal of Clinical Microbiology","volume":" ","pages":"e0036225"},"PeriodicalIF":5.4,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12239718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Performance evaluation of a commercial multiplex pathogen panel for the diagnosis of pediatric joint infections. 一种商用复合病原体检测试剂盒用于小儿关节感染诊断的性能评价。
IF 6.1 2区 医学
Journal of Clinical Microbiology Pub Date : 2025-07-09 Epub Date: 2025-06-02 DOI: 10.1128/jcm.00278-25
Lawson Copley, Gina Lee, Mary Villani, Naureen Tareen, Laura M Filkins
{"title":"Performance evaluation of a commercial multiplex pathogen panel for the diagnosis of pediatric joint infections.","authors":"Lawson Copley, Gina Lee, Mary Villani, Naureen Tareen, Laura M Filkins","doi":"10.1128/jcm.00278-25","DOIUrl":"10.1128/jcm.00278-25","url":null,"abstract":"<p><p>This study evaluates the performance of the BIOFIRE Joint Infection (JI) Panel compared to joint fluid culture and/or 16S rRNA PCR, followed by Sanger sequencing (16S PCR/S) for the diagnosis of joint infections in pediatric patients. An on-panel organism was detected by standard-of-care joint (SOCj) studies (joint fluid culture with or without 16S PCR/S, as ordered by the treating physician) in 29/65 samples (44.6%), and the same organism was detected by the BIOFIRE JI panel in all samples. The cumulative positive percent agreement in the detection of on-panel genus or species-level targets by the BIOFIRE JI panel was 100% (36/36), 100% (25/25), and 100% (27/27), and the negative percent agreement was 99.7% (1,974/1,979), 99.2% (1,974/1,990), and 99.7% (1,303/1,307) compared to detection by SOCj, joint fluid culture only, or 16S PCR/S only, respectively. The potential clinical impact of employing the BIOFIRE JI panel was predicted by retrospective adjudication using a study-specific rubric. We predicted that 27.7% (18/65) of BIOFIRE JI panel results could have had a positive impact on patient care. One case (1.5%) was predicted to potentially have a negative impact, and three cases (4.6%) were adjudicated to have an unknown impact. All organisms detected by 16S PCR/S, but not by culture, were also detected by the BIOFIRE JI panel during this study.</p><p><strong>Importance: </strong>The BIOFIRE JI Panel has been limitedly evaluated in pediatric patients. Our study shows a strong agreement between the BIOFIRE JI panel and culture and/or 16S rRNA PCR with Sanger sequencing for the detection of the most common pathogenic causes of joint infection in children. The faster time to results of the BIOFIRE JI panel has the potential to guide optimal treatment faster than conventional methods.</p>","PeriodicalId":15511,"journal":{"name":"Journal of Clinical Microbiology","volume":" ","pages":"e0027825"},"PeriodicalIF":6.1,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12239719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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