PCR sensitivity for Mycoplasma pneumoniae detection in nasopharyngeal and oropharyngeal swabs: a comparative study.

IF 6.1 2区 医学 Q1 MICROBIOLOGY
Daisuke Kitagawa, Shin Nishihara, Masayuki Murata, Mai Onishi, Takahiro Mori, Soshi Hachisuka, Tenshin Okubo, Naohiro Yamamoto, Hiroki Nishikawa, Masayuki Onaka, Rika Suzuki, Soma Suzuki, Ayu Yamamoto, Ritsuki Uejima, Fumihiko Nakamura, Sayaka Yoshida, Taito Kitano
{"title":"PCR sensitivity for <i>Mycoplasma pneumoniae</i> detection in nasopharyngeal and oropharyngeal swabs: a comparative study.","authors":"Daisuke Kitagawa, Shin Nishihara, Masayuki Murata, Mai Onishi, Takahiro Mori, Soshi Hachisuka, Tenshin Okubo, Naohiro Yamamoto, Hiroki Nishikawa, Masayuki Onaka, Rika Suzuki, Soma Suzuki, Ayu Yamamoto, Ritsuki Uejima, Fumihiko Nakamura, Sayaka Yoshida, Taito Kitano","doi":"10.1128/jcm.00458-25","DOIUrl":null,"url":null,"abstract":"<p><p>The differential impact of sample type on polymerase chain reaction (PCR) detection of <i>Mycoplasma pneumoniae</i> (MP) has rarely been investigated. The study aimed to evaluate the diagnostic performance of PCR for the detection of MP and to measure MP DNA load between nasopharyngeal and oropharyngeal swabs. Nasopharyngeal and oropharyngeal samples were obtained simultaneously to evaluate their diagnostic performance in children with suspected MP. Two commercially available PCR tests, multiplex PCR and Smart Gene Myco, were used to analyze the nasopharyngeal and oropharyngeal samples, respectively. Furthermore, real-time PCR (RT-PCR) tests were conducted on both sample residues to validate the results. In total, 422 participants underwent simultaneous PCR testing using nasopharyngeal and oropharyngeal swabs; 139 samples (32.9%) from nasopharyngeal swabs and 176 samples (41.7%) from oropharyngeal samples that tested positive using commercially available tests. RT-PCR tests were positive for 136 (32.2%) nasopharyngeal and 183 (43.4%) oropharyngeal residual samples. With the RT-PCR test of the residual extract from oropharyngeal swabs as a reference, the sensitivity and specificity of detecting MP were 74.9% (95% confidence interval 67.9%-81.0%) and 99.2% (97.0%-99.9%) with the multiplex PCR test on nasopharyngeal swabs, and 96.2% (92.3%-98.4%) and 100.0% (98.5%-100.0%) with the Smart Gene Myco on oropharyngeal samples. A negative correlation was observed between fluoroquinolone use and oropharyngeal DNA loads (<i>P</i> = 0.004). The sensitivity of MP detection was significantly better in oropharyngeal samples than in nasopharyngeal samples. This study indicates that oropharyngeal samples should be used to detect MP rather than nasopharyngeal samples.IMPORTANCEObtaining the best sample is crucial for the accurate diagnosis of <i>Mycoplasma pneumoniae</i> (MP) and timely and appropriate treatment. This study aimed to assess the diagnostic performance of MP detection using polymerase chain reaction (PCR) tests between nasopharyngeal and oropharyngeal samples. This study showed that the sensitivity of detecting MP was 74.9% (95% confidence interval 67.9%-81.0%) with a commercially available PCR test on nasopharyngeal swabs, and 96.2% (92.3%-98.4%) with a commercially available PCR test on oropharyngeal samples. The sensitivity of MP detection was significantly better in oropharyngeal samples than in nasopharyngeal samples. This study supports the idea that oropharyngeal samples should be used to detect MP. The results contribute to guidance in the recommendation regarding sampling methods to detect MP. Accurate identification of MP is crucial not only for timely and appropriate antimicrobial treatment but also for efficient epidemiological surveillance.</p>","PeriodicalId":15511,"journal":{"name":"Journal of Clinical Microbiology","volume":" ","pages":"e0045825"},"PeriodicalIF":6.1000,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Clinical Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1128/jcm.00458-25","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The differential impact of sample type on polymerase chain reaction (PCR) detection of Mycoplasma pneumoniae (MP) has rarely been investigated. The study aimed to evaluate the diagnostic performance of PCR for the detection of MP and to measure MP DNA load between nasopharyngeal and oropharyngeal swabs. Nasopharyngeal and oropharyngeal samples were obtained simultaneously to evaluate their diagnostic performance in children with suspected MP. Two commercially available PCR tests, multiplex PCR and Smart Gene Myco, were used to analyze the nasopharyngeal and oropharyngeal samples, respectively. Furthermore, real-time PCR (RT-PCR) tests were conducted on both sample residues to validate the results. In total, 422 participants underwent simultaneous PCR testing using nasopharyngeal and oropharyngeal swabs; 139 samples (32.9%) from nasopharyngeal swabs and 176 samples (41.7%) from oropharyngeal samples that tested positive using commercially available tests. RT-PCR tests were positive for 136 (32.2%) nasopharyngeal and 183 (43.4%) oropharyngeal residual samples. With the RT-PCR test of the residual extract from oropharyngeal swabs as a reference, the sensitivity and specificity of detecting MP were 74.9% (95% confidence interval 67.9%-81.0%) and 99.2% (97.0%-99.9%) with the multiplex PCR test on nasopharyngeal swabs, and 96.2% (92.3%-98.4%) and 100.0% (98.5%-100.0%) with the Smart Gene Myco on oropharyngeal samples. A negative correlation was observed between fluoroquinolone use and oropharyngeal DNA loads (P = 0.004). The sensitivity of MP detection was significantly better in oropharyngeal samples than in nasopharyngeal samples. This study indicates that oropharyngeal samples should be used to detect MP rather than nasopharyngeal samples.IMPORTANCEObtaining the best sample is crucial for the accurate diagnosis of Mycoplasma pneumoniae (MP) and timely and appropriate treatment. This study aimed to assess the diagnostic performance of MP detection using polymerase chain reaction (PCR) tests between nasopharyngeal and oropharyngeal samples. This study showed that the sensitivity of detecting MP was 74.9% (95% confidence interval 67.9%-81.0%) with a commercially available PCR test on nasopharyngeal swabs, and 96.2% (92.3%-98.4%) with a commercially available PCR test on oropharyngeal samples. The sensitivity of MP detection was significantly better in oropharyngeal samples than in nasopharyngeal samples. This study supports the idea that oropharyngeal samples should be used to detect MP. The results contribute to guidance in the recommendation regarding sampling methods to detect MP. Accurate identification of MP is crucial not only for timely and appropriate antimicrobial treatment but also for efficient epidemiological surveillance.

鼻咽拭子和口咽拭子检测肺炎支原体的PCR敏感性比较研究。
不同样品类型对肺炎支原体(MP)聚合酶链反应(PCR)检测的差异影响很少被研究。本研究旨在评价PCR检测MP的诊断性能,并测定鼻咽拭子和口咽拭子之间的MP DNA载量。同时采集鼻咽和口咽标本,评价其对疑似MP患儿的诊断效果。多重PCR和Smart Gene Myco两种市售PCR检测方法分别用于分析鼻咽和口咽样本。此外,对两种样品残留物进行实时荧光定量PCR (RT-PCR)检测以验证结果。总共有422名参与者使用鼻咽和口咽拭子同时进行PCR检测;139个鼻咽拭子样本(32.9%)和176个口咽样本(41.7%)经市售检测呈阳性。鼻咽残留标本136份(32.2%)、口咽残留标本183份(43.4%)呈RT-PCR阳性。以鼻咽拭子残提物RT-PCR检测为参照,鼻咽拭子多重PCR检测MP的灵敏度和特异性分别为74.9%(95%置信区间67.9% ~ 81.0%)和99.2% (97.0% ~ 99.9%),Smart Gene Myco检测MP的灵敏度和特异性分别为96.2%(92.3% ~ 98.4%)和100.0%(98.5% ~ 100.0%)。氟喹诺酮类药物的使用与口咽DNA负荷呈负相关(P = 0.004)。口咽标本MP检测的敏感性明显优于鼻咽标本。本研究提示MP检测应采用口咽标本而非鼻咽标本。重要意义获得最佳标本对肺炎支原体(MP)的准确诊断和及时治疗至关重要。本研究旨在评估聚合酶链反应(PCR)检测鼻咽和口咽样本中MP的诊断性能。本研究表明,市售的鼻咽拭子PCR检测MP的灵敏度为74.9%(95%置信区间67.9% ~ 81.0%),口咽拭子PCR检测MP的灵敏度为96.2%(92.3% ~ 98.4%)。口咽标本MP检测的敏感性明显优于鼻咽标本。本研究支持口咽样本用于检测MP的观点。结果有助于指导建议的抽样方法,以检测MP。准确识别MP不仅对及时和适当的抗微生物治疗至关重要,而且对有效的流行病学监测也至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Clinical Microbiology
Journal of Clinical Microbiology 医学-微生物学
CiteScore
17.10
自引率
4.30%
发文量
347
审稿时长
3 months
期刊介绍: The Journal of Clinical Microbiology® disseminates the latest research concerning the laboratory diagnosis of human and animal infections, along with the laboratory's role in epidemiology and the management of infectious diseases.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信