Journal of cyclic nucleotide and protein phosphorylation research最新文献_第3页

The escape of cyclic AMP from dog thyroid slices exposed to positive and negative regulators. 暴露于正、负调节剂下的狗甲状腺片环AMP的逃逸。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

P Cochaux, J Van Sande, S Swillens, J E Dumont

{"title":"The escape of cyclic AMP from dog thyroid slices exposed to positive and negative regulators.","authors":"P Cochaux, J Van Sande, S Swillens, J E Dumont","doi":"","DOIUrl":"","url":null,"abstract":"Incubation of dog thyroid slices with 1 mU/ml TSH resulted in enhanced intracellular and extracellular cAMP accumulation. In the absence of TSH, the intra- and extracellular cAMP concentrations remained at a constant low level. The release of cAMP from TSH-stimulated slices was inhibited by 10 microM PGA1, 1 mM probenecid or 1 mM IBMX, which are known inhibitors of cAMP escape in several tissues. Negative controls of intracellular cAMP levels are exerted in the dog thyroid by 10 microM carbamylcholine (shown to activate a Ca++- calmodulin dependent phosphodiesterase), 100 microM norepinephrine and 100 microM iodide (both inhibiting adenylate cyclase activity). The purpose of the present study was to demonstrate that these three agents do not enhance cAMP escape. The results presented here show that these agents decrease both intracellular accumulation and escape in parallel. Moreover, the escape constants obtained by numerical simulation were not greater in the presence of inhibiting concentrations of carbamylcholine, norepinephrine or iodide. Thus the inhibition by these agents of cAMP accumulation in TSH-stimulated dog thyroid slices cannot be explained by a stimulation of cAMP escape from these cells.","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":"11 2","pages":"75-85"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13574985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cholinergic attenuation of the electrophysiological effects of forskolin. 福斯克林电生理效应的胆碱能衰减。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

G M Wahler, N Sperelakis

引用次数: 0

Kinetic evidence indicating separate stimulatory and inhibitory prostaglandin receptors on platelet membranes. 动力学证据表明血小板膜上有单独的刺激和抑制前列腺素受体。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

B Ashby

{"title":"Kinetic evidence indicating separate stimulatory and inhibitory prostaglandin receptors on platelet membranes.","authors":"B Ashby","doi":"","DOIUrl":"","url":null,"abstract":"Progress curves of cyclic AMP production by human platelet lysates in the presence of 10 microM GppNp were upward curving, reaching a steady-state rate after about 20 min. Increasing concentrations of prostaglandin E1 reduced the lag time required to reach steady-state and increased the steady-state rate in a dose-dependent manner. At 0.3 microM PGE1 the progress curve was linear for at least 40 min. At higher concentrations of PGE1 the initial rate of cyclic AMP formation was linear and similar to that obtained at 1.0 microM PGE, however, the progress curve showed a downward curvature after several minutes, reaching a new steady-state rate after about 10 min. The extent of downward curvature was dose-dependent, and at 10 microM PGE1 the final steady-state rate had almost returned to that observed in the presence of GppNp alone. Analysis of initial and final steady-state rates as a function of prostaglandin concentration revealed two apparently saturable processes that were interpreted as binding to a stimulatory receptor (EC50 = 130 nM), followed by binding to a lower affinity inhibitory receptor (EC50 = 1200 nM) that showed a slow response to receptor occupancy. Qualitatively similar results were obtained with PGD2 and the stable PGI2 analog ZK36374.","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":"11 4","pages":"291-300"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13578303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of binding of 125I-iodopindolol to control and desensitized cells at 37 degrees and on ice. 碘碘多洛在37度和冰上与对照和脱敏细胞结合的比较。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

M L Toews, G L Waldo, T K Harden, J P Perkins

{"title":"Comparison of binding of 125I-iodopindolol to control and desensitized cells at 37 degrees and on ice.","authors":"M L Toews, G L Waldo, T K Harden, J P Perkins","doi":"","DOIUrl":"","url":null,"abstract":"Binding of 125I-iodopindolol (IPIN) to intact 1321N1 human astrocytoma cell B-adrenergic receptors was measured at 37 degrees and on ice. Control cells showed a single component of IPIN binding on ice with the same total number of receptors as measured at 37 degrees. In desensitized cells (pretreated for 20 min with 1 microM isoproterenol) approximately 40% of IPIN binding on ice exhibited kinetics similar to those observed in control cells. The remaining 60% of receptors were labelled by IPIN at a much slower rate requiring the use of very high concentrations of IPIN. Sucrose density gradient fractionation was used to separately study the labelling of plasma membrane receptors and those associated with a light vesicle fraction. Labelling by IPIN on ice of the plasma membrane receptors of control cells was rapid, labelling of the light vesicle receptors of desensitized cells was slow, and labelling of the plasma membrane receptors of desensitized cells appeared to occur with both rapid and slow components. Selective labelling of the plasma membrane receptors of intact cells thus could be obtained by incubation with IPIN on ice under selected conditions. Similar results were obtained when broken cell preparations from control and desensitized cells were used. The decreased binding of IPIN on ice to B-adrenergic receptors in the light vesicle fraction not only provides further evidence consistent with sequestration of B-adrenergic receptors during desensitization, it also provides a convenient and inexpensive means to assay the sequestration reaction.","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":"11 1","pages":"47-62"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14143197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adenylate cyclase in sarcoplasmic reticulum of skeletal muscle: distribution, orientation, and regulation. 骨骼肌肌浆网腺苷酸环化酶:分布、定位和调控。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

M Nakagawa, J H Willner

引用次数: 0

Do porins inhibit the macrophage phagocyting activity by stimulating the adenylate cyclase? 孔蛋白是否通过刺激腺苷酸环化酶抑制巨噬细胞的吞噬活性?

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

A Di Donato, G F Draetta, G Illiano, M A Tufano, L Sommese, F Galdiero

引用次数: 0

Mitogenic signalling and protein phosphorylation in Xenopus oocytes. 非洲爪蟾卵母细胞的有丝分裂信号传导和蛋白磷酸化。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

J L Maller

{"title":"Mitogenic signalling and protein phosphorylation in Xenopus oocytes.","authors":"J L Maller","doi":"","DOIUrl":"","url":null,"abstract":"Xenopus oocytes are stimulated to undergo meiotic cell division in response to several types of mitogenic stimuli. Agents that reduce cAMP levels induce cell division in oocytes, and this occurs due to inhibition of adenylate cyclase with progesterone as well as by activation of phosphodiesterase with insulin. Phorbol esters and microinjected protein kinase C also promote cell division, implicating phospholipid breakdown as another signalling pathway competent to induce proliferation in this system. A third signalling pathway is via the tyrosine kinase activity of the insulin receptor. A proximal activation of a ribosomal protein S6 kinase by insulin has provided insight into the regulation of this pathway. All three of these signal transduction pathways lead to the activation of a cytoplasmic protein able to induce nuclear breakdown, chromosome condensation and spindle formation in vivo and in vitro. This protein, known as maturation-promoting factor, is associated with changes in protein phosphorylation on both serine and tyrosine residues. These results support a model in which signal transduction by different pathways activates a common cell cycle control element that regulates the G2----M transition via changes in protein phosphorylation.","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":"11 7","pages":"543-55"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13969694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Action spectra of photoactivated cyclic GMP metabolism and relaxation in bovine mesenteric artery. 牛肠系膜动脉光激活环GMP代谢和松弛的作用谱。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

J O Karlsson, K L Axelsson, H Elwing, R G Andersson

{"title":"Action spectra of photoactivated cyclic GMP metabolism and relaxation in bovine mesenteric artery.","authors":"J O Karlsson, K L Axelsson, H Elwing, R G Andersson","doi":"","DOIUrl":"","url":null,"abstract":"Strips of bovine mesenteric arteries, brought to sustained contraction by addition of 3.0 microM phenylephrine, relaxed during exposure to shortwave light. The action spectrum of the photorelaxation was characterized by most effectiveness of relaxation in the range of 360 to 400 nm; on the contrary, shortwave light of 280 to 300 nm increased the tension. The photo-induced relaxation was accompanied by an increase in the cGMP level, measured 30 sec after the onset of radiation, and the action spectrum of the increase in cGMP seemed to coincide fairly well with the action spectrum of relaxation. Both the increase in cGMP and relaxation in response to increasing light intensity at 400 nm fitted the rectangular hyperbolic equation. The K values (the intensity which gave half maximal response) for cGMP increase and relaxation, respectively, obtained by non-linear least square regression analysis, were found to assume very close values (1.3 mW/cm2 for cGMP increase and 1.5 mW/cm2 for relaxation). The action spectrum of the crude soluble guanylate cyclase (GC) activity displayed a peak around 400 nm. Our results suggest that there is a close association between photo-induced increase in cGMP and relaxation, probably as a result of interaction between shortwave light and the heme moiety of GC.","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":"11 3","pages":"155-66"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14013846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of thyrotropin-stimulated adenosine 3',5'-monophosphate formation in rat thyroid cells by an adenosine analog. Evidence that the inhibition is mediated by the putative inhibitory guanine nucleotide regulatory protein. 腺苷类似物抑制促甲状腺素刺激的大鼠甲状腺细胞中3'，5'-单磷酸腺苷的形成。证据表明抑制是由假定的抑制性鸟嘌呤核苷酸调节蛋白介导的。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

M I Berman, C G Thomas, S N Nayfeh

{"title":"Inhibition of thyrotropin-stimulated adenosine 3',5'-monophosphate formation in rat thyroid cells by an adenosine analog. Evidence that the inhibition is mediated by the putative inhibitory guanine nucleotide regulatory protein.","authors":"M I Berman, C G Thomas, S N Nayfeh","doi":"","DOIUrl":"","url":null,"abstract":"Addition of N6-(L-2-phenylisopropyl)-adenosine (PIA) to cultured FRTL-5 rat thyroid cells led to a concentration-dependent inhibition of TSH-stimulated cAMP formation. Half-maximal inhibition was attained with approximately 0.5 nM PIA. Forskolin and cholera toxin-stimulated cAMP production were also inhibited by PIA. 3-Isobutyl-methylxanthine inhibited the effect of PIA. These results are consistent with the presence of inhibitory adenosine receptors (Ri). Ri-sites were further demonstrated by the binding of 3H-cyclohexyl-adenosine to FRTL-5 plasma membranes. High (Kd = 0.50 +/- 0.07 nM) and low affinity (Kd = 5.95 +/- 2.33 nM) binding sites were observed. Pretreatment of FRTL-5 cells with pertussis, but not cholera, toxin effectively antagonized the inhibitory effects of PIA on cAMP production. ADP-ribosylation of FRTL-5 membranes with [32P]-NAD in the presence of cholera or pertussis toxin specifically labeled a 45,000 and 41,000 Mr species, respectively, which correspond to the alpha subunit of the stimulatory and inhibitory guanine nucleotide regulatory proteins. These results demonstrate that PIA inhibits TSH-stimulated cAMP production via Ri-sites on FRTL-5 thyroid cells. PIA appears to exert its inhibitory effects through the inhibitory guanine nucleotide regulatory protein.","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":"11 2","pages":"99-111"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14148797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Second phosphorylation site on alpha subunit of eIF-2 in rabbit reticulocyte lysate. 兔网织细胞裂解液中eIF-2 α亚基的第二个磷酸化位点。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

R Jagus, B Safer

{"title":"Second phosphorylation site on alpha subunit of eIF-2 in rabbit reticulocyte lysate.","authors":"R Jagus, B Safer","doi":"","DOIUrl":"","url":null,"abstract":"Eukaryotic protein synthesis initiation factor 2, eIF-2, was purified from either hemin-supplemented (translationally active) or hemin-deficient (translationally inactive) rabbit reticulocyte lysate under conditions chosen and demonstrated to preserve the in situ phosphorylation state. Direct analysis of the phosphate content of the alpha-subunit of eIF-2 was determined by chemical analysis of the isolated alpha-subunit or by a combination of vertical slab gel isoelectric focusing and immunoblotting. These results were compared with those obtained from an indirect analysis utilising the incorporation of [gamma-32P]ATP into eIF-2 by the heme-sensitive eIF-2 alpha-kinase. All three analyses demonstrate that the phosphorylation site specific for the heme-sensitive kinase is unoccupied in translationally active lysate and 25-30% occupied in translationally inhibited lysate. In addition, both direct analyses support the existence of a second phosphorylation site on the alpha-subunit, not regulated by hemin and distinct from that phosphorylated by the heme-sensitive kinase. Different reticulocyte lysate batches vary with respect to the activity of the kinase responsible for phosphorylation of the second site. Further investigations demonstrated that this kinase is a membrane-associated protein.","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":"11 7","pages":"557-70"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14563728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0