Journal of Clinical and Experimental Dentistry最新文献

{"title":"An Exuberant Calcifying Epithelial Odontogenic Tumor in the Posterior Maxilla: A Case Report.","authors":"Rodrigo Silva, Carolina Ruppel, Ramon Cesar Godoy Gonçalves, Roberto de Oliveira Jabur, Ana Luiza Oliveira Corrêa Roza, Pablo Agustin Vargas, Marcelo Carlos Bortoluzzi","doi":"10.4317/jced.62701","DOIUrl":"10.4317/jced.62701","url":null,"abstract":"<p><p>This case report presents a rare instance of a Calcifying Epithelial Odontogenic Tumor (CEOT) in the maxilla of a 52-year-old male, characterized by a painless, growing mass and facial asymmetry. Imaging revealed extensive involvement of the maxillary sinus, nasal cavity, pterygoid plate, and infraorbital margin. Histopathological analysis confirmed the diagnosis, showing polyhedral odontogenic epithelium with amyloid-like material. The patient underwent conservative surgical excision, preserving function and aesthetics, with no recurrence observed at 16 months postoperatively. This case underscores the importance of a multidisciplinary approach in managing CEOT, particularly in complex anatomical regions, to achieve favorable outcomes. Long-term follow-up remains essential. <b>Key words:</b>Calcifying Epithelial Odontogenic; Pindborg tumor; CEOT; maxilla; odontogenic tumors.</p>","PeriodicalId":15376,"journal":{"name":"Journal of Clinical and Experimental Dentistry","volume":"17 5","pages":"e599-e603"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12142358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imaging features of a brown tumor with extensive skull involvement: Relevance for dental radiology.","authors":"Artemisa Fernanda Moura Ferreira, Francisco de Assis Limeira-Júnior, José Jhenikártery Maia de Oliveira, Paulo Rogerio Ferreti Bonan, Marcelo Augusto Oliveira de Sales","doi":"10.4317/jced.62709","DOIUrl":"10.4317/jced.62709","url":null,"abstract":"<p><p>This case report describes a rare, asymptomatic brown tumor in a patient with end-stage renal disease. The lesion was incidentally detected during a computed tomography (CT) scan of the paranasal sinuses, performed upon a dentist's recommendation to investigate maxillary sinusitis. CT imaging revealed an expansive osteolytic lesion with irregular margins and a ground-glass appearance involving the left side of the sphenoid and frontal sinuses. Subsequent magnetic resonance imaging (MRI) with multiplanar T1 and T2-weighted sequences without contrast demonstrated a solid tissue-like expansive lesion affecting the left frontal and sphenoid bones, mildly compressing adjacent cerebral parenchyma. Despite these findings, the patient remained asymptomatic. Conservative management, including pharmacological therapy with calcimimetics to control parathyroid hormone levels, was initiated. A follow-up MRI after five years showed lesion stability without significant changes. The patient later underwent a renal transplant, which effectively stabilized the bone disease and improved his quality of life. This case underscores the pivotal role of computed tomography (CT) in detecting incidental systemic skeletal changes and the indispensable importance of interdisciplinary collaboration in managing complex conditions in systemically compromised patients, where each professional's expertise is crucial for the patient's well-being. <b>Key words:</b>Sinusitis, Brown Tumor, Hyperparathyroidism, Multidetector Computed Tomography, Magnetic Resonance Imaging, Multidisciplinary Care Teams.</p>","PeriodicalId":15376,"journal":{"name":"Journal of Clinical and Experimental Dentistry","volume":"17 5","pages":"e604-e607"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12142367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of cone-beam computed tomography and endodontic practitioners' proficiency level on diagnosis and treatment planning of root resorption.","authors":"Emel Olga Onay, Derin Bugu Yuzer, Eda Cakmak, Kamran Gulsahi","doi":"10.4317/jced.62382","DOIUrl":"10.4317/jced.62382","url":null,"abstract":"<p><strong>Background: </strong>A precise diagnosis is essential for formulating an effective treatment plan for both internal and external resorptions. Cone-beam computed tomography (CBCT) offers a three-dimensional view of the maxillofacial area, capturing images from coronal, axial, and sagittal angles. This method overcomes the limitations of conventional intraoral radiography (IR), especially when it comes to detecting and identifying defects related to internal and external resorption. Thus, the aim of this study was to assess whether CBCT imaging affects the accuracy of diagnosing and planning treatment for internal and external resorption defects differently between endodontic residents (ERs) and specialists (ESs).</p><p><strong>Material and methods: </strong>Thirty-five clinicians reviewed 3 internal and 3 external resorption cases using clinical histories and intraoral radiographs (IRs), then answered questions about their diagnosis and treatment decisions. One month later, they re-evaluated the cases with CBCT and answered similar questions. Data analyzed using Mc-Nemar chi-square test and Prevalence Adjusted Bias Adjusted Kappa statistic. The level of statistical significance was set off <i>p</i>< 0.05 in all data.</p><p><strong>Results: </strong>CBCT significantly improved diagnostic accuracy in 2 out of 6 cases (<i>p</i>< 0.001) and altered the treatment plan in 4 out of 6 cases (<i>p</i>< 0.05). There was no significant difference between ERs and ESs regarding diagnosis and treatment planning using the same imaging technique (<i>p</i>> 0.05).</p><p><strong>Conclusions: </strong>This study suggests that CBCT provides more detailed information compared to IR, with both imaging techniques allowing ERs and ESs to achieve similar diagnostic and treatment planning accuracy. <b>Key words:</b>Clinical decision making, cone-beam computed tomography, dental radiography, diagnosis, root resorption.</p>","PeriodicalId":15376,"journal":{"name":"Journal of Clinical and Experimental Dentistry","volume":"17 5","pages":"e515-e520"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12142370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral viral, fungal, and bacterial infections linked to comorbidities: A case series from a Brazilian referral center.","authors":"Daniella Estanho, Lucas Fellipe do Amaral-Sobrinho, Fernanda Silva de Lima, João Pedro Silva Contreiras, Michelle Agostini, Natália Silva Andrade, José Alcides Almeida de Arruda, Sandra R Torres, Sílvia Paula de Oliveira, Bruno Augusto Benevenuto de Andrade, Jefferson R Tenório","doi":"10.4317/jced.62619","DOIUrl":"https://doi.org/10.4317/jced.62619","url":null,"abstract":"<p><strong>Background: </strong>Oral infections exhibit variability in their causative agents and clinical presentations, underscoring the necessity of accurate diagnosis for effective management. Despite extensive documentation globally, data on these infections from Brazil remain scarce. This study aimed to assess the occurrence, clinical features, and differential diagnosis of oral viral, fungal, and bacterial infections at a single center in southern Brazil.</p><p><strong>Material and methods: </strong>A retrospective analysis was conducted between 2010 and 2023. Clinicodemographic data, comorbidities, and routine medication use were analyzed descriptively and statistically.</p><p><strong>Results: </strong>A total of 462 cases were included. The median age was 49.5 years (range: 2-100). Viral infections were the most frequent (65.8%), with squamous papilloma accounting for 49.4% of cases. Fungal infections comprised 29.4% of cases, predominantly erythematous candidiasis (20.8%) and pseudomembranous candidiasis (5.6%). These infections were more common in women, older adults (<i>p</i><0.001), and individuals with comorbidities such as systemic arterial hypertension (<i>p</i>=0.006) and diabetes mellitus (<i>p</i>=0.028). Bacterial infections were rare (4.8%), with actinomycosis being the most frequent (2.2%).</p><p><strong>Conclusions: </strong>Data from our series on oral viral, fungal, and bacterial infections align with the literature. The results emphasize the importance of tailored diagnostic approaches, particularly for at-risk patient populations. <b>Key words:</b>Bacterial infections, Communicable diseases, Mycoses, Oral manifestations, Virus diseases.</p>","PeriodicalId":15376,"journal":{"name":"Journal of Clinical and Experimental Dentistry","volume":"17 4","pages":"e382-e392"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077827/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single cone and bioceramic sealer in oval canals: Efficiency endodontic obturation.","authors":"Nicolás Collado-Castellanos, Pedro Micó-Muñoz, Alberto Albero-Monteagudo, Annayancy Castañeda-Argueta, Mahnoor Aamir, Vicente Faus-Llácer","doi":"10.4317/jced.62633","DOIUrl":"https://doi.org/10.4317/jced.62633","url":null,"abstract":"<p><strong>Background: </strong>To compare the volume of voids in oval canals obturated with single cone and bioceramic sealer (SCBC), single cone with lateral condensation or Guttacore by means of micro- computed tomography analysis. To measure the obturation time used for each technique.</p><p><strong>Material and methods: </strong>65 uni-radicular teeth with an oval canal were selected. Canals were shaped with Protaper Next and divided into three groups according to the tested obturation technique. The time taken to obturate the canals was recorded. Each specimen was scanned using micro-CT at a voxel resolution of 25 µm. The volume of detected voids and volume of obturated canal was calculated in relation to the total volume. Voids were classified according to their location in the radicular thirds. 2D images obtained were used to calculate the percentage of filled area and percentage of voids. ANOVA test was used to assess significant differences in voids detected in the root thirds and obturation time. Multiple comparisons were made with the Bonferroni test. To analyze the root thirds Kruskal-Wallis was used and for pairwise comparisons Mann-Whitney with Bonferroni modification.</p><p><strong>Results: </strong>The highest percentage of void volume and void area was found in the single cone group with lateral condensation technique. These differences were significant in the apical third (<i>p</i><0,01). SCBC was significantly faster (<i>p</i><0.001).</p><p><strong>Conclusions: </strong>The three-obturation techniques achieved low void rates. SCBC had the least number of voids and was the fastest obturation technique. Clinical Relevance: In oval canals the quality of obturation carried out with these techniques were suitable and clinically acceptable. SCBC was the most efficient obturation technique. <b>Key words:</b>Bioceramic sealer, Oval canals, Single cone technique, Root canal filling, Micro-CT analysis, Guttacore Obturator.</p>","PeriodicalId":15376,"journal":{"name":"Journal of Clinical and Experimental Dentistry","volume":"17 4","pages":"e399-e406"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bond Strength of Silane-Containing Universal Bonding Agents to Lithium Disilicate.","authors":"Daniel L Tannen, Adam J Wallum, Terrell M Mitchell, James J Renda, Kraig S Vandewalle","doi":"10.4317/jced.62011","DOIUrl":"https://doi.org/10.4317/jced.62011","url":null,"abstract":"<p><strong>Background: </strong>The purpose of this study was to evaluate the microshear bond strength to lithium disilicate of four silane-containing universal adhesives (SUAs): Scotchbond Universal Adhesive (3M), Scotchbond Universal Adhesive Plus (3M), Clearfil Universal Bond Quick (Kuraray), and Universal Bond II (Tokuyama), with or without the use of a separate silane primer.</p><p><strong>Material and methods: </strong>Lithium disilicate blocks (IPS e.max CAD, Ivoclar) were sectioned to create 4 equal partitions. Blocks were then crystallized and mounted in polyvinyl chloride (PVC) pipes. The blocks were steam cleaned, subjected to 5% hydrofluoric acid etch, rinsed, and prepared with or without silane application. Resin cement was applied to prepared blocks within standardized silicone tubing matrices and light-cured forming four resin cement specimens per block and twelve specimens per group (n=12). The dual-cure resin cement with and without the use of silane served as a positive and negative control, respectively. The tubing was removed and then the specimens were subjected to 500 thermocycles. The specimens were loaded perpendicularly in a universal testing machine with a shear force until bonding failure. The mean microshear bond strength was calculated per group and analyzed with ANOVA and Tukey's post hoc tests (alpha=0.05). Following testing, each specimen was examined to determine failure mode.</p><p><strong>Results: </strong>Except for Universal Bond II, an application of silane resulted in a significant increase in bond strength (<i>p</i><0.05) when using the SUAs. Without the use of a silane, Universal Bond II had significantly greater bond strength (<i>p</i><0.0001) than all other SUAs. All the SUAs without the use of silane resulted in lower bond strength (except UB) compared to the typical clinical practice of the use of silane alone. Greater mixed and cohesive failures were observed with groups that used silane.</p><p><strong>Conclusions: </strong>Silane-containing universal bonding agents are being marketed in lieu of silane application on the intaglio surface of lithium disilicate prior to cementation. The results from this study indicate that using SUAs (except Universal Bond II) in this manner may decrease bond strength of the interface between lithium disilicate and resin cement. <b>Key words:</b>Universal bonding agent, silane, lithium disilicate, bond strength.</p>","PeriodicalId":15376,"journal":{"name":"Journal of Clinical and Experimental Dentistry","volume":"17 4","pages":"e358-e365"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological effect of fluoride varnishes on teeth with caries in the white spot phase: An <i>in vitro</i> study.","authors":"Margot Margarita Gutiérrez-Ilave, Rocío Del Pilar Ríos-León, Antonia Castro-Rodríguez, María Rosario Calixto-Cotos","doi":"10.4317/jced.62010","DOIUrl":"https://doi.org/10.4317/jced.62010","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to compare the histological remineralization effects of two fluoride varnishes on artificially produced caries lesions in young permanent teeth.</p><p><strong>Material and methods: </strong>Twenty longitudinal sections of premolars with artificially produced white spot lesions and evaluated with a polarised light microscope. The sample consisted of 20 tooth fragments, which were divided and assigned to two groups: The first group with application of Clinpro TM White Varnish (20 tooth sections) and the second, with MI Varnish TM (20 tooth sections) subjected for 6 days to conditions similar to the oral cavity. The sections were then re-evaluated by calculating the lesion depth and remineralization area (Image J software). Student' s t-test was used to analyse the data.</p><p><strong>Results: </strong>MI Varnish TM fluoride varnish achieved a greater remineralization area than Clinpro TM White Varnish (838042.6±140359.3 μm2 and 678313.8±137265.7μm2, respectively), with a statistically significant difference (<i>p</i><0.05).</p><p><strong>Conclusions: </strong>MI Varnish TM had a better effect than Clinpro TM White Varnish in remineralizing lesions, in vitro, in the enamel of young permanent teeth with caries in the white-stained phase. <b>Key words:</b>Fluorides varnishes, histological effect, dental remineralization, polarised light microscope.</p>","PeriodicalId":15376,"journal":{"name":"Journal of Clinical and Experimental Dentistry","volume":"17 4","pages":"e416-e421"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myofunctional Responses in Obstructive Sleep Apnea Syndrome in Children Following the Use of Two Oral Orthopedic Devices.","authors":"Ana Beatriz Bueno Carlini Bittencourt, Clóvis Lamartine de Moraes Melo-Neto, Gabriela Aparecida Dos Santos, Emily Vivianne Freitas da Silva, Claúdia Sanae Akita Shimoide Muraoka, André Pinheiro de Magalhães Bertoz, Daniela Micheline Dos Santos, Marcelo Coelho Goiato","doi":"10.4317/jced.62603","DOIUrl":"https://doi.org/10.4317/jced.62603","url":null,"abstract":"<p><strong>Background: </strong>Rapid maxillary expansion (RME) is less invasive and an efficient method of treatment for obstructive sleep apnea syndrome (OSAS). Objective: To assess the therapeutic impact of oral orthopedic appliances in the treatment of obstructive sleep apnea syndrome with polysomnography (PSG), electromyography (EMG), bite force measurement (BFM), questionnaires, and cephalometric analysis.</p><p><strong>Material and methods: </strong>Eleven children (aged seven to eleven years) underwent three months of treatment with the Hyrax device, followed by the installation of the Balter´s Bionator appliance. Quality analysis, type III polysomnography (PSG), electromyography (EMG), and bite force measurement (BFM) were conducted. The analysis were performed through eleven months of treatment. The distributional normality were verified by the Kolmogorov-Smirnov, For these data, the Student test was performed. For data with non-normal distribution, the Wilcoxon test was performed. All analyzes were performed with a significance level of 5%.</p><p><strong>Results: </strong>Polysomnography data showed that severe scores became moderate or mild with the use of the devices. Electromyography, during rest periods, demonstrated that only the right temporal muscle exhibited increased electrical activity, while both muscles showed increased activity during dental clenching. There was an increase in the masseter and right temporalis during grape chewing. Bite Force Measurement (BFM) did not reveal any statistical difference before and after treatment. Questionnaire responses showed a statistically significant difference in the Sleep Disturbance Scale and OSA-18-PV.</p><p><strong>Conclusions: </strong>It can be concluded that the use of the Balters Hyrax and Bionator devices in class II children and children with OSAS is a safe and effective treatment alternative. <b>Key words:</b>Sleep apnea obstructive, bite force, polysomnography, electromyography, oral health, children.</p>","PeriodicalId":15376,"journal":{"name":"Journal of Clinical and Experimental Dentistry","volume":"17 4","pages":"e393-e398"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Evaluation of Autologous Platelet Aggregates Versus Blood Clot On The Outcome Of Regenerative Endodontic Therapy: A Systematic Review and Meta-Analysis.","authors":"Saumya Verma, Alpa Gupta, Mrinalini Mrinalini, Dax Abraham, Unnati Soma","doi":"10.4317/jced.62508","DOIUrl":"https://doi.org/10.4317/jced.62508","url":null,"abstract":"<p><strong>Background: </strong>Regenerative endodontics represents a transformative approach to dental care, revitalizing necrotic teeth. This systematic review and meta-analysis evaluated the role of autologous platelet aggregates compared to the traditional blood clot method in regenerative endodontics.</p><p><strong>Material and methods: </strong>A systematic search was conducted in PubMed, Scopus, EBSCO, Open Grey, and Google Scholar between 1st-12th August 2024. Case series, RCTs, retrospective studies, and case reports were included. Meta-analysis on RCTs and case series utilized RevMan 5.4 software, with <i>p</i>=0.05 as the significance level. The JBI risk of bias tool and GRADE system assessed study quality.</p><p><strong>Results: </strong>Nineteen studies met inclusion criteria, of which 13 were evaluated for risk of bias-11 showed low risk, and 2 were moderate. Rates of complete apical closure using PRF, BC, PRP, and CGF scaffolds ranged from 61.76% to 100%. Statistical analysis revealed no significant differences between autologous platelet aggregates and BC for outcomes such as complete apical closure (BC vs. PRP: <i>p</i>=0.28; BC vs. PRF: <i>p</i>=0.36), positive vitality (BC vs. PRP: <i>p</i>=0.70; BC vs. PRF: <i>p</i>=0.36), healing response (BC vs. PRF: <i>p</i>=0.23), and overall success score (BC vs. PRP: <i>p</i>=0.62).</p><p><strong>Conclusions: </strong>BC remains an effective primary scaffold for non-vital teeth with open apexes. PRP and PRF are viable alternatives when intracanal blood induction is challenging. Overall, platelet aggregates and BC showed comparable clinical and radiographic outcomes. <b>Key words:</b>Apexogenesis, Autologous Platelet Aggregates, Blood Clot, Immature Tooth, Regenerative Endodontics.</p>","PeriodicalId":15376,"journal":{"name":"Journal of Clinical and Experimental Dentistry","volume":"17 4","pages":"e447-e460"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of solutions and storage time on the chemical and mechanical properties of human dentin.","authors":"Fernanda Castelo Branco Santos Bettero, Camila de Carvalho Almança Lopes, Gabriel Júlio Guerra, Veridiana Resende Novais","doi":"10.4317/jced.62433","DOIUrl":"https://doi.org/10.4317/jced.62433","url":null,"abstract":"<p><strong>Background: </strong>Considering the importance of standardization of the pH of control solutions and its impact on the chemical composition and mechanical properties of dentin during storage over a specified period, this study aimed to analyze the pH of control solutions and how it affects the chemical composition and mechanical properties of dentin stored over a given period.</p><p><strong>Material and methods: </strong>Six control solutions-coconut water, mineral water, distilled water, deionized water, artificial tears, and saline-were kept in a bacteriological incubator at 37°C, with their pH measured using a pH meter (mPA-210 from MS Tecnopon ®) for seven days: T0 (initial), T1 (2 hours), T2 (24 hours), T3 (48 hours), and T4 (7 days). In the second phase, the two solutions with the most stable pH in the first phase were selected and aligned with the critical pH of dentin (pH ≥ 6.5). Human third molars were sectioned and divided into two groups (n=13): distilled water and deionized water. The pH of the solutions, the chemical composition, and the microhardness of the dentin were evaluated by pH meter, Fourier transform infrared spectroscopy (FTIR), and Knoop microhardness (KH), respectively, at the aforementioned time points. The values were analyzed by two-way ANOVA (storage time and solution), followed by the Tukey test.</p><p><strong>Results: </strong>Both solutions presented pH incompatible with the dentin until T2, and the samples presented a 40% reduction in microhardness at T4. Additionally, a reduction in carbonate and an increase in amides were observed in the dentin, indicating changes in the mineral and organic phases.</p><p><strong>Conclusions: </strong>It is concluded that both the solutions and the storage time negatively affect the chemical composition and microhardness of the dentin, highlighting the importance of carefully selecting the control solutions and the storage time in in vitro studies. <b>Key words:</b>Dentin, hardness, FTIR, pH, storage solution.</p>","PeriodicalId":15376,"journal":{"name":"Journal of Clinical and Experimental Dentistry","volume":"17 4","pages":"e374-e381"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}