Journal of Chemical Ecology最新文献

{"title":"Herbicide Stress Modifies Defensive, Nutritional, and Volatile Chemistry of Red Maple Cultivars Associated with Chrysobothris Attraction.","authors":"Cindy Perkovich, Melanie Eichler, Paul O'Neal, Karla M Addesso","doi":"10.1007/s10886-026-01743-1","DOIUrl":"10.1007/s10886-026-01743-1","url":null,"abstract":"<p><p>Herbicide exposure can alter plant secondary metabolism with consequences for insect host selection, yet its effects on volatile organic compound (VOC) signaling remain poorly understood. Red maple (Acer rubrum L.) is a widely planted ornamental tree frequently attacked by flatheaded borers (Chrysobothris spp.), and herbicide injury has been associated with increased borer activity. We investigated how trunk application of two commercial herbicides, one contact herbicide (pelargonic acid) and one systemic herbicide (glyphosate), influence VOC emissions and associated nutritional and defensive chemistry in two red maple cultivars differing in susceptibility to Chrysobothris. Across a four-week field experiment, foliar VOCs were quantified alongside sugars, polyphenols, and tannins, and related to borer oviposition attempts and larval success. Herbicide exposure induced compound-specific and cultivar-dependent VOC responses, with the susceptible cultivar ('Brandywine') exhibiting greater temporal variability and elevated emissions of select monoterpenes, including β-myrcene. These VOC shifts coincided with increased foliar polyphenols and trunk sugar concentrations. In contrast, the less susceptible cultivar ('Autumn Blaze') showed comparatively stable chemical profiles across treatments. Oviposition attempts and larval occurrence were concentrated on herbicide-treated 'Brandywine' trees, particularly following contact herbicide exposure. Together, these results suggest that herbicide-induced modulation of VOC signaling, coupled with changes in nutritional and defensive chemistry, may influence adult host selection, larval performance following oviposition, and ultimately enhance host suitability and apparency to Chrysobothris.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":"52 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148591742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spotted Lanternfly Feeding Induces Systemic Defense Responses in Grapevines.","authors":"Sukhman Singh, Michelle Peiffer, Gary W Felton, Flor E Acevedo","doi":"10.1007/s10886-026-01742-2","DOIUrl":"10.1007/s10886-026-01742-2","url":null,"abstract":"<p><p>The spotted lanternfly (Lycorma delicatula), an invasive planthopper in the United States, threatens vineyards by reducing yield and compromising plant health, yet grapevine immune responses to its feeding remain poorly understood. This study evaluated systemic grapevine responses to spotted lanternfly feeding under varying insect densities and feeding durations. Potted Vitis vinifera cultivar Cabernet Franc vines were infested with 0, 5, 10, or 15 adults per vine for 1, 3, 5, 7, 14, 21, or 29 days, using separate plants at each time point. Leaf tissue was analyzed for chlorophyll content, polyphenol oxidase (PPO) and peroxidase (POX) activity, protein content, total phenolics, and condensed tannins. Bioassays assessed spotted lanternfly survival and weight gain on previously infested plants. Chlorophyll content declined after 21-29 days in vines infested with 15 insects. PPO activity increased throughout the experiment (except on day 7) under infestations of 10-15 insects, whereas POX activity rose after 7 days but declined after 29 days of feeding. Leaf protein content increased in vines infested with 15 insects after 7 and 29 days, and phenolic concentrations increased consistently from 7 to 29 days in vines infested with 10-15 insects. Condensed tannin concentrations were unaffected by density treatments. Bioassays showed reduced survival and weight gain of lanternflies when fed on vines previously infested with 15 insects for 29 days. Collectively, these results suggest that prolonged, high-density spotted lanternfly feeding induces coordinated, time- and density-dependent systemic grapevine defenses associated with reduced host suitability. Future studies should identify the mechanisms underlying these responses and their persistence across grapevine cultivars.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":"52 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13400602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148584542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"(3E,5Z)-Octadien-2-ol is an Aggregation-Sex Pheromone Produced by Males of the Queensland Longhorned Beetle, Acalolepta aesthetica (Olliff 1890).","authors":"Matthew D Ginzel, Jorden Zarders, Kyeongnam Kim, Ellen J Dunkle, Kylle Roy, Dong H Cha, Jocelyn G Millar","doi":"10.1007/s10886-026-01735-1","DOIUrl":"10.1007/s10886-026-01735-1","url":null,"abstract":"<p><p>We describe the identification, synthesis, and field testing of an aggregation-sex pheromone produced by male Acalolepta aesthetica (Olliff 1890) (Coleoptera: Cerambycidae: Lamiinae), a cerambycid beetle native to Australia that has recently become established in Hawai'i. Comparing extracts of headspace volatiles from both sexes revealed that males emitted two sex-specific volatiles, (3E,5Z)-octadien-2-ol and (3E,5Z)-octadien-2-one. Both compounds were synthesized and their bioactivity was tested in field bioassays. Only (3E,5Z)-octadien-2-ol was attractive to beetles of both sexes in these trials, and the corresponding ketone appeared to antagonize attraction when combined with the alcohol. In a dose-response study, traps baited with all doses of (3E,5Z)-octadien-2-ol except 33.3 mg captured more beetles than solvent-control traps, with no significant differences among the effective doses. In a subsequent field trial, beetle capture did not differ between 100 mg of a racemic blend and the insect-produced (R)-enantiomer of this compound, indicating that the unnatural (S)-enantiomer is not antagonistic. No other cerambycid species were captured during our field trials. The structural motif of (3E,5Z)-octadien-2-ol is unique among pheromones identified from lamiines to date. Together, our findings provide a foundation for developing pheromone-based tools to detect, delimit and potentially manage A. aesthetica.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":"52 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diving Into the Chemical World of Cetaceans: A Review on the Chemical Senses in Dolphins and Whales.","authors":"Pablo Pezzino, Sylvia Campagna, Aurélie Célérier","doi":"10.1007/s10886-026-01737-z","DOIUrl":"10.1007/s10886-026-01737-z","url":null,"abstract":"<p><p>In evolutionary terms, chemical senses are the oldest sensory modality and play key roles in ecological functions such as orientation, foraging, and socio-sexual communication. Chemical perception encompasses olfaction, taste, and chemesthesis, and has been widely studied across vertebrates. However, these senses remain poorly documented in marine mammals, particularly cetaceans. The transition from land to water profoundly reshaped cetacean sensory systems, and for decades they were considered incapable of chemoreception, implying a complete regression. Recent studies challenge this view, suggesting a more complex and incomplete regression. In this review, we synthesize current knowledge from different perspectives, using an integrative framework. First, we examine the anatomical basis of chemoreception, identifying relevant structures enabling this process and their development across early life stages. Second, we explore genetic and molecular foundations, focusing on genes underlying the development and function of chemical receptors. Third, we review behavioural evidence, highlighting experimental studies on cetacean responses to chemical cues in ecological contexts such as predator-prey interactions, foraging, and social behaviour. This approach allows us to characterize and quantify chemosensory regression in cetaceans and confirms divergent evolutionary trajectories between odontocetes and mysticetes. We also identify key unresolved questions: (1) cetaceans appear to use social chemical signals, but the mechanisms remain unknown; (2) reliance on taste is still debated, with conflicting findings; (3) the ontogeny of chemical senses remains poorly understood; and (4) alternative chemical modalities such as chemesthesis have been largely overlooked despite hypothesis on their potential ecological importance.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":"52 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148470841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Behavioral and Exumbrellar Integumental Defenses in Jellyfish: The Hydromedusan Spirocodon saltatrix Releases Oxidized Derivatives of Unsaturated Fatty Acids Potentially to Avoid Predation and Bacterial Biofouling.","authors":"Nana Miura, Ryogo Suzuki, Koto Shimotori, Kazufumi Osako, Yudai Akimoto, Takeshi Terahara, Gaku Yamamoto, Aya Adachi, Haruto Ishii, Kazuya Fukuhara, Hiroshi Nagai, Michiya Kamio","doi":"10.1007/s10886-026-01740-4","DOIUrl":"10.1007/s10886-026-01740-4","url":null,"abstract":"<p><p>Cnidarian jellyfish-the medusa phase of medusozoans-are gaining recognition as important prey for a variety of predators in marine trophic webs. However, their traits as prey remain poorly understood. In this study, we examined the defensive traits of jellyfish, focusing specifically on cnidocyte-independent chemical defenses that serve as strategies against consumers, pathogens, and biofouling. To investigate these anti-consumer chemical defenses, we selected three sympatric jellyfish species: the hydromedusan Spirocodon saltatrix and the scyphomedusan moon jellyfish Aurelia coerulea as prey, and the scyphomedusan Japanese sea nettle Chrysaora pacifica as the predator. Using these species, we conducted feeding experiments, ablation of potential defensive organs, and analyses of compounds released by the prey. We found that: (1) in feeding experiments, S. saltatrix individuals exhibited an involution behavior, in which they strongly contracted and retracted their tentacles inside the umbrella when captured by C. pacifica, thereby escaping, whereas A. coerulea individuals were consumed without escaping; (2) a series of surgical ablation experiments indicated that the umbrella integument of S. saltatrix possesses a defensive function; (3) gas chromatography-mass spectrometry (GC-MS) analyses showed that S. saltatrix releases C8 to C9 alcohols and aldehydes when its integument is damaged; (4) behavioral experiments demonstrated that these compounds deter feeding by the predatory jellyfish; (5) these compounds inhibited biofilm formation by the sympatric bacterium Ectopseudomonas sp. isolated from sediment, indicating their antifouling role, though their effects on planktonic biofouling remain to be tested; and (6) the experimentally determined effective concentrations were higher than the bulk measured concentrations. Therefore, we hypothesize that the natural microscale concentrations of these compounds at the injured integument are sufficiently high to match the effective thresholds, suggesting that they function as defensive agents. Thus, our study demonstrates that S. saltatrix utilizes involution behavior and its integument to avoid predation. To our knowledge, this is the first report of exumbrellar integumental defense in a cnidarian jellyfish with suggestive evidence of chemical defense using oxidized derivatives of unsaturated fatty acids.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":"52 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13356075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148420762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucosinolate-Rich Floral Extracts Hinder Nosema (= Vairimorpha) ceranae Infection in Caged Apis mellifera Workers.","authors":"Matteo Carloni, Eleonora Pagnotta, Laura Righetti, Lorena Malaguti, Vittorio Capano, Antonio Nanetti, Nicola Pecchioni, Manuela Bagatta, Luisa Ugolini","doi":"10.1007/s10886-026-01736-0","DOIUrl":"10.1007/s10886-026-01736-0","url":null,"abstract":"<p><p>Colony losses in Apis mellifera are caused by multiple stressors, among which the parasitic microsporidian Nosema (= Vairimorpha) ceranae. As an alternative to the controversial fumagillin, sustainable control strategies based on natural bioactive compounds are increasingly being explored. This study evaluated the efficacy of glucosinolate (GSL)-based formulations derived from flowers of Eruca sativa and Reseda lutea in controlling N. ceranae infection in worker honey bees under laboratory conditions. Freeze-dried flowers were characterized for their GSL profile and myrosinase activity, and formulation studies were conducted to choose the best material for bioassays. Then, water-soluble GSL-enriched extracts were produced. Cage feeding assays were performed on uninfected newly emerged bees using sugar syrup supplemented with 2 and 4 µmol g⁻¹ of total GSL of either formulation. Extract administration reduced food consumption and bee survival in a concentration-dependent manner compared to the control group. The lower concentration was further tested on 6-day-old bees artificially infected with N. ceranae spores, showing consumption comparable to control, but still a slight decrease in survival. At this concentration, both E. sativa- and R. lutea-based extracts significantly inhibited N. ceranae abundance, by 23.8 and 44.6%, respectively, with no significant difference between plant species. Isothiocyanates-the GSL hydrolysis products-were detected in bee intestines, accumulating primarily in the hindgut and at higher levels in bees fed with R. lutea extracts. These findings highlight the potential of floral GSL-rich formulations as natural, environmentally sustainable tools to support colony health and reduce pathogen load.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":"52 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13350151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148412016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scents and Scent Ability: Can Snakes Identify Heterospecifics and Conspecifics Using Airborne Semiochemicals?","authors":"Rulon W Clark, Harshita Vallabhaneni, Hana M Koyama, Elisa J Paider, Donald M Walker, Jason W Dallas","doi":"10.1007/s10886-026-01738-y","DOIUrl":"10.1007/s10886-026-01738-y","url":null,"abstract":"<p><p>All animals produce semiochemicals (i.e., scent)-chemical cues that elicit physiological or behavioral responses in other individuals. Scent detection and discrimination is a fundamental aspect of the social system of many animal species, and is particularly important for snakes and other squamate reptiles. Snakes rely extensively on chemosensory information in most aspects of their life, and have a highly derived tongue-vomeronasal sensory system that is active when exploring novel chemical cues. While the role of non-volatile semiochemicals in snake behavior has received a great deal of attention, measuring how volatile, airborne semiochemicals influence behavior has been understudied due to methodological hurdles. To explore this knowledge gap, we developed a novel bioassay to test the ability of snakes to discriminate among airborne semiochemicals from other individuals. Initial experiments using this bioassay demonstrate that prairie rattlesnakes (Crotalus viridis) can use airborne semiochemicals to discriminate between conspecifics and heterospecifics (gopher snakes, Pituophis catenifer), but not between different conspecific individuals. We discuss the further implications of these findings, highlighting the utility of our experimental approach for addressing future questions related to the relationships between semiochemical production, perception, and social behavior.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":"52 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148404855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variable Secondary Metabolites for Defense Against Chilo Partellus (Swinhoe) and Sesamia Inferens (Walker) in Maize.","authors":"Mukesh Kumar Dhillon, Tanmaya Kumar Bhoi, Ashok Kumar Sau, Sengottayan Senthil-Nathan","doi":"10.1007/s10886-026-01739-x","DOIUrl":"10.1007/s10886-026-01739-x","url":null,"abstract":"<p><p>Inbuilt biochemical plant defense is the most reliable and sustainable solution to combat insect pest attacks. The stem borers, Chilo partellus (Swinhoe) and Sesamia inferens (Walker), are major threats to maize production in tropical countries of the world. Present study was carried out on host selection behaviour, antibiosis and induced biochemical defense against these borer species. The study revealed that CPM 15 and CML 345 were less preferred, took greater time in host establishments, and exhibited antibiosis against C. partellus and S. inferens in comparison to other test genotypes. Damage caused by Sesamia inferens resulted in higher levels of total proteins, sugars, starch, carotenoids, phenols, ferric ion reducing powder, antioxidants, catalase, ascorbate peroxidase, ascorbate oxidase, phenylalanine ammonia lyase (PAL), and tyrosine ammonia lyase (TAL) compared to C. partellus across the test genotypes. However, C. partellus damage increased, and S. inferens decreased total lipids in all the test genotypes. In the case of C. partellus, 97.6% variability in larval preference was explained by catalase and ascorbate oxidase; 93.4% variability in host establishment time by total proteins, sugars and phenols; and 93.5% variability in antibiosis by total proteins, sugars and ascorbate peroxidase. However, 96.7% variability in larval preference was explained by total proteins, sugars, phenols and PAL; 96.1% variability in time to establish by starch alone; and 91.9% variability in antibiosis by ascorbate oxidase and TAL in the case of S. inferens. Finally, although more than 90% of the variability in larval preference and antibiosis was explained by various induced biochemicals, no common biomolecule was identified among the test genotypes that could defend against C. partellus and S. inferens.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":"52 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148404833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Matador Bug and Several Related Leaf-footed Bugs (Hemiptera: Coreidae) Accumulate Cyanogens From their Host Plants.","authors":"Colin R Morrison, Brian E Sedio, Lawrence E Gilbert, Justin C Havird, Ummat Somjee","doi":"10.1007/s10886-026-01730-6","DOIUrl":"10.1007/s10886-026-01730-6","url":null,"abstract":"<p><p>Cyanogenic compounds provide potent defenses to herbivorous insects, yet few species are known to acquire them from their food. Matador bugs, Bitta alipes (Hemiptera: Coreidae), have brightly colored signals that deter avian predators and are specialists of cyanogenic Passiflora plants. It is unknown whether these insects accumulate cyanogenic compounds. We document the first evidence of cyanogen accumulation in matador bugs. However, the presence and concentration of cyanogens depended on the Passiflora species from which insects were feeding. Further, three related species of inconspicuous Coreidae, and their Cactaceae and Aquifoliaceae host plants, also contained cyanogenic compounds. In all coreid species, cyanogen concentrations detected would make them unpalatable, or even lethal, to vertebrate predators. We report the first evidence of cyanogenic compounds in the Coreidae, doubling the number of species known to accumulate cyanide in the Hemiptera. Cyanogen accumulation may be more common amongst hemipterans than previously thought, prompting studies to examine the mechanisms of secondary chemical accumulation and evolution of aposematism.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":"52 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13346313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148404788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating a Semiochemical-Based Mechanism for the Beneficial Activity of Sorghum Intercropped with Soybeans on Management of Popillia Japonica.","authors":"Nicole B Kucherov, Matthew C Hetherington, Alison R Gerken, William R Morrison, Tania N Kim","doi":"10.1007/s10886-026-01734-2","DOIUrl":"10.1007/s10886-026-01734-2","url":null,"abstract":"<p><p>Japanese beetle, Popillia japonica Newman, is an invasive species that attacks many field and ornamental crops. Intercropping soybean with sorghum is suggested as a strategy to reduce the abundance of P. japonica throughout soybean fields. The mechanism by which this affects P. japonica is unknown but may arise from a difference in the host plant compounds emitted by the intercropped plants because P. japonica responds to olfactory cues. In this study, we investigated a semiochemical-based mechanism for the behavioral response of P. japonica to intercropping soybean with sorghum by (1) evaluating P. japonica behavioral responses to solvent extracts from monocropped soy, intercropped soy, and a mixture of soy and sorghum extracts in no-choice video-tracking and preference in a release-recapture dual-choice assay to host stimuli from plants; and (2) applying solid-phase microextraction (SPME) in conjunction with gas chromatography coupled with mass spectrometry (GC-MS) to characterize the semiochemical profiles of each extract. We found unique semiochemical profiles among our treatments, with 1-octen-3-ol and 1-octanol characteristic of monocropped soybean and dodecane-1-iodo primarily in sorghum extracts. Nevertheless, our treatments did not significantly affect movement or orientation by P. japonica compared to controls, nor did conspecifics exhibit a significant preference for any of the treatments in a dual-choice assay at a local scale. Therefore, if intercropping soybean with sorghum significantly affects the behavior of P. japonica in the field, it may occur at a different scale, or in response to non-olfactory stimuli (e.g., visual, habitat, or landscape cues) rather than semiochemical cues.</p>","PeriodicalId":15346,"journal":{"name":"Journal of Chemical Ecology","volume":"52 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148387582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}