B. Galligan, Ryan Riling, Jessica Stufflet, A. Swarup, C. Sunkari, David Alfego, A. Kriete
{"title":"Peptide-Based Delivery System used for Tagging Syk Tyrosine Kinase Biomarker in Skin Cancer and Development of a Hyperspectral Imaging Device to Detect the Biomarker","authors":"B. Galligan, Ryan Riling, Jessica Stufflet, A. Swarup, C. Sunkari, David Alfego, A. Kriete","doi":"10.1109/NEBEC.2013.125","DOIUrl":"https://doi.org/10.1109/NEBEC.2013.125","url":null,"abstract":"We propose to design a non-invasive diagnostic method for the early detection of SCC and BCC using a hyperspectral imaging device that will detect fluorescence. The technique used to deliver the fluorescent tag will be in the form of a topical formulation consisting of a Syk-specific peptide conjugated to a fluorescent dye encapsulated in liposomes.","PeriodicalId":153112,"journal":{"name":"2013 39th Annual Northeast Bioengineering Conference","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125821773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The HapticMaster: Rendering Haptics in MATLAB","authors":"K. Abbruzzese, R. Foulds","doi":"10.1109/NEBEC.2013.144","DOIUrl":"https://doi.org/10.1109/NEBEC.2013.144","url":null,"abstract":"The FCS HapticMaster is a 3 degree of freedom robot that incorporates an admittance control paradigm. This device is capable of rendering haptic objects through the use of a HapticAPI. However, the HapticAPI relies on C++ source code and OpenGL for graphical display. MATLAB executable (MEX) functions may offer an alternative approach for rendering haptics. These subroutines allow MATLAB to execute C++ code directly in MATLAB, as if they were MATLAB functions. These MEX functions grant the ability to use the HapticMaster in MATLAB, gaining access to all the positive features of the software. This has prompted the development of virtual reality therapies in MATLAB that incorporate haptic features.","PeriodicalId":153112,"journal":{"name":"2013 39th Annual Northeast Bioengineering Conference","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128482246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
X. Cai, Brian J. Roscoe, Z. Alamgir, S. Sani, R. Wang, S. Schuckers, Alexandru Stoian, Charles J. Robinson
{"title":"Latencies of Cardiac-Linked Seismic Pulses in Head Accelerometer Measurements","authors":"X. Cai, Brian J. Roscoe, Z. Alamgir, S. Sani, R. Wang, S. Schuckers, Alexandru Stoian, Charles J. Robinson","doi":"10.1109/NEBEC.2013.107","DOIUrl":"https://doi.org/10.1109/NEBEC.2013.107","url":null,"abstract":"A 60 s time-series record of AP, RL and Z-axes data is taken from a head-mounted 3-axis accelerometer, along with a 3-lead ECG signal. The R-wave peak is then used as a trigger to conduct spike-triggered averaging of the accelerometer data. As a result, a cardiac-linked acceleration waveform appears. The latencies of these resultant waves are compared to the latencies produced when an accelerometer is placed on the chest wall.","PeriodicalId":153112,"journal":{"name":"2013 39th Annual Northeast Bioengineering Conference","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130198594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Nano-BaSO4: A Novel Bacteriostatic Polymer Additive","authors":"G. Aninwene, T. Webster","doi":"10.1109/NEBEC.2013.117","DOIUrl":"https://doi.org/10.1109/NEBEC.2013.117","url":null,"abstract":"Hospital acquired infections remain a major costly problem. This study sought to understand how incorporating nano-barium sulfate into pellethane composites affect the physical properties and antimicrobial nature of the resulting polymers. The results of this study showed that the incorporation of nano-barium sulfate into pellethane polymers yielded polymers which had enhanced antimicrobial properties, yet had similar hydrodynamic properties compared to pellethane polymers with standard barium sulfate.","PeriodicalId":153112,"journal":{"name":"2013 39th Annual Northeast Bioengineering Conference","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130557912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bacteria Fighting Paper Towels: The Influence of Selenium Nanoparticles","authors":"Qi Wang, T. Webster","doi":"10.1109/NEBEC.2013.55","DOIUrl":"https://doi.org/10.1109/NEBEC.2013.55","url":null,"abstract":"Bacterial infections are commonly found on paper towels and other paper products leading to the potential spread of bacteria and consequent health concerns. The objective of this in vitro study was to introduce antibacterial properties to standard paper towel surfaces by coating them with selenium nanoparticles. Scanning electron microscopy was used to measure the size and distribution of the selenium coatings on the paper towels. The amount of selenium precipitated on the paper towels was measured by atomic absorption spectroscopy. In vitro bacterial studies with S. aureus were conducted to assess the effectiveness of the selenium coatings at inhibiting bacterial growth. Results showed that the selenium nanoparticles coated on the paper towel surfaces were well distributed and were semi spherical 50nm in diameter. Most importantly, the selenium nanoparticle coated paper towels inhibited S. aureus growth by 90% after 24, 48 and 72 hours compared with uncoated paper towels. Thus, this study showed that nano-selenium coated paper towels may lead to an increased eradication of bacteria to clean a more wide-range of clinical environments and for the food industry, thus, improving health.","PeriodicalId":153112,"journal":{"name":"2013 39th Annual Northeast Bioengineering Conference","volume":"418 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132442378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Williams, V. Douard, D. Bajaj, Y. Sabbagh, R. Ferraris, J. C. Fritton
{"title":"Low Calcium and High Fructose Diet Diminish the Quality of Circumferential Long-Bone Growth","authors":"E. Williams, V. Douard, D. Bajaj, Y. Sabbagh, R. Ferraris, J. C. Fritton","doi":"10.1109/NEBEC.2013.109","DOIUrl":"https://doi.org/10.1109/NEBEC.2013.109","url":null,"abstract":"High fructose (HF) intake is a public health problem. However, little is known about effects on bone quality during growth. Greater data exists for low calcium (Ca2+). Low-Ca (0.02%) or normal calcium and calorie-equivalent glucose or fructose diets (43%) were fed to male, 3-wk-old, B6 mice for 6 wks (4 groups; n=9/group). Weight and long-bone lengths were not affected. Low-Ca diet resulted in less stiff and strong (~40%; p<;0.01) long bone and increased toughness (energy absorption) of long-bone mid-shaft, measured by 3-point bending; post-yield displacement was increased in low Ca by >65% (p<;0.05). By histomorphometry, low Ca resulted in decreased (>20%, p<;0.05) cortical width, partially explaining the bending results. Only the glucose-fed group exhibited compensation to maintain outside circumference and diameter by increased periosteal bone formation rate (p.BFR); 3-fold greater vs. each of the other 3 groups (p<;0.05). One plausible mechanism for poor circumferential growth involves bone in a feedback loop and is suggested by decreased intestinal Ca transport and Ca transporter protein in mucosa. The fructose diet completely prevented both adaptive increases in Ca transport, and 1α-hydroxylase-mediated synthesis of 1,25(OH)2D3 levels.","PeriodicalId":153112,"journal":{"name":"2013 39th Annual Northeast Bioengineering Conference","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129266150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hayin Candiotti, Brian Karl, K. Knowles, D. Mathews, C. Hall, Kyle Mohen, M. Paliwal
{"title":"Fused Deposition Modeling BioPrinter","authors":"Hayin Candiotti, Brian Karl, K. Knowles, D. Mathews, C. Hall, Kyle Mohen, M. Paliwal","doi":"10.1109/NEBEC.2013.98","DOIUrl":"https://doi.org/10.1109/NEBEC.2013.98","url":null,"abstract":"Scaffolds with three-dimensional, customizable architectures will be fabricated and populated with fibroblasts via automated processes of a Fused Deposition Modeling BioPrinter. The success of the design will be dependent on the accuracy of fabrication and adhesion/proliferation of the seeded fibroblasts.","PeriodicalId":153112,"journal":{"name":"2013 39th Annual Northeast Bioengineering Conference","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125408262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of Low Oxygen Tension during Expansion on Chondrogenic Potential of Osteoarthritic Chondrocytes","authors":"Jing Wang, K. A. Davis, J. H. Henderson","doi":"10.1109/NEBEC.2013.157","DOIUrl":"https://doi.org/10.1109/NEBEC.2013.157","url":null,"abstract":"Primary human chondrocytes, although a promising cell source for cartilage tissue engineering, tend to dedifferentiate and lose their characteristic gene expression and protein production during monolayer expansion. Low O2 tension in vitro culture has been studied to examine its effect on preventing dedifferentiation, and results have been conflicting. In particular, low O2 expansion effects on human osteoarthritis (OA) chondrocytes are poorly understood. In this study, we expanded chondrocytes collected from patients with osteoarthritis (OA) under normal (21%) and low (5%) O2 tension then continued with micromass culture under 21% O2 for 3 weeks. Our results suggest that low O2 condition may promote chondrogenic characteristic gene expression in monolayer cells. But improved ECM production, which was seen earlier in animal models, may not be relevant to human OA chondrocytes. Further examination of the mechanical properties of the engineered pellets is needed to confirm the effects of low O2 expansion on human OA chondrocytes and their use in cartilage tissue engineering.","PeriodicalId":153112,"journal":{"name":"2013 39th Annual Northeast Bioengineering Conference","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116056649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cardiac Fibroblast-Formed Anisotropic Decellularized Engineered Cardiac Tissues","authors":"V. Vejseli, E. Lee","doi":"10.1109/NEBEC.2013.4","DOIUrl":"https://doi.org/10.1109/NEBEC.2013.4","url":null,"abstract":"Although structural anisotropy is important for proper function of normal myocardium, it has been a challenge to mimic structural architecture of native tissue in vitro. Previous studies have focused on using two-dimensional cultures utilizing micropatterning of substrates. Here, we present three-dimensional (3D) decellularized engineered tissues with a controlled varying degree of extracellular matrix alignment. Engineered tissues were initially created by embedding cardiac fibroblasts in 3D collagen tissues (rat tail type I collagen, BD sciences) and cultured either under biaxial or uniaxial constraints. Biaxially constrained tissues yielded a random orientation of both cell and collagen fibers, while uniaxially constrained tissues showed that the cell and collagen fibers aligned parallel to the constrained direction. To facilitate cardiac fibroblast deposition of extracellular matrix, culture medium was supplemented with 5 ng/ml ascorbic acid every 3 days. Once desired structural alignment was obtained, cells were removed by decellurization treatment using CHAPS buffer followed by SDS buffer for 1 hr at 37°C with gentle agitation. Removal of cells was confirmed by DNA quantification and intact collagen fiber orientation was confirmed by confocal reflected light microscopy. This study demonstrates the feasibility of creating cell-formed structural alignment combined with decellularization technique to establish decellularized anisotropic engineered tissue, which can be used to evaluate the effects of biophysical cues on stem cell or stem cell-derived cardiomyocyte differentiation and function in vitro.","PeriodicalId":153112,"journal":{"name":"2013 39th Annual Northeast Bioengineering Conference","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116148490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Infrared Spectroscopy to Measure Collagen and Elastin in Aorta Using Multivariate Analysis","authors":"R. Cheheltani, C. Mcgoverin, M. Kiani, N. Pleshko","doi":"10.1109/NEBEC.2013.103","DOIUrl":"https://doi.org/10.1109/NEBEC.2013.103","url":null,"abstract":"Pathological changes in extracellular matrix (ECM) occur in the context of abdominal aortic aneurysm disease. The objective of this study is to assess the capability of Fourier transform infrared spectroscopy (FTIR) to differentiate major aorta ECM components. Two partial least squares (PLS) models were developed using mixtures of matrix components in potassium bromide pellets. In the first model collagen and elastin powders were mixed in varying percentages. In the second model, pig aorta specimens were selectively degraded by enzymatic treatment of either collagen or elastin and used to prepare pellets. The results of the PLS model showed the ability of FTIR methodology for differentiation of elastin and collagen. These results lay the foundation for application of the methodology to assess the progression of AAA pathology.","PeriodicalId":153112,"journal":{"name":"2013 39th Annual Northeast Bioengineering Conference","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115779533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}